Article
Nanoscience & Nanotechnology
Shruthi Polla Ravi, Yasmeen Shamiya, Aishik Chakraborty, Ali Coyle, Alap Ali Zahid, Jin Wang, Michael S. H. Boutilier, Emmanuel A. Ho, Arghya Paul
Summary: In this study, a biomimetic cell-derived nanoparticle (CDN) loaded with Dexamethasone was developed, which showed efficient cellular uptake and stem cell differentiation. These CDNs exhibited good cytocompatibility and stability, making them suitable for bone regeneration applications.
ACS APPLIED NANO MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Liren Wang, Jia Jiang, Hai Lin, Tonghe Zhu, Jiangyu Cai, Wei Su, Jiebo Chen, Junjie Xu, Yamin Li, Jing Wang, Kai Zhang, Jinzhong Zhao
Summary: Regenerative sports medicine aims to treat sports and aging-related conditions using tissue regeneration techniques. This review summarizes the optimal regeneration techniques for each area and assesses the time required for feasible clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Fatima Garcia-Villen, Idoia Gallego, Patricia Galvez-Martin, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This article reviews the advantages of using 3D bioprinting techniques for osteochondral regeneration, discusses the commonly used biomaterials, cell types, and active molecules, and considers the most recent promising results.
Review
Nanoscience & Nanotechnology
Xu Li, Bingyang Dai, Jiaxin Guo, Lizhen Zheng, Quanyi Guo, Jiang Peng, Jiankun Xu, Ling Qin
Summary: The review discusses the pathological features of osteoarthritis, the design concept for accurate drug localization within cartilage, interactions of nanoparticles with cartilage microenvironment, and advances in therapeutic schemes. The emphasis is on developing smart bioresponsive and multiple modality nanoparticles to interact with pathological signals.
NANO-MICRO LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Fernando Canonici, Cristiano Cocumelli, Antonella Cersini, Daniele Marcoccia, Alessia Zepparoni, Annalisa Altigeri, Daniela Caciolo, Cristina Roncoroni, Valentina Monteleone, Elisa Innocenzi, Cristian Alimonti, Paola Ghisellini, Cristina Rando, Eugenia Pechkova, Roberto Eggenhoeffner, Maria Teresa Scicluna, Katia Barbaro
Summary: Cartilage injuries in animals and humans can lead to the progression of osteoarthritis and joint deterioration. A new experimental protocol for treating osteochondral lesions involves isolating chondrocytes from equine hyaline cartilage and using them to repair equine joint cartilage injuries. The chondrocytes, taken from autologous tracheal cartilage, are grown in a laboratory and implanted arthroscopically into the injured joint. Histological and immunohistochemical evaluation of the treated cartilage after 8 and 13 months shows progressive maturation, with tissue comparable to native articular cartilage detected after 24 months.
Review
Chemistry, Multidisciplinary
Constance Lesage, Marianne Lafont, Pierre Guihard, Pierre Weiss, Jerome Guicheux, Vianney Delplace
Summary: The osteochondral (OC) unit is essential for joint lubrication and bone constraint transmission. Currently, there is no curative treatment for OC defects, making OC regeneration a medical challenge. Over the past two decades, tissue engineering strategies have been proposed, but successful clinical translation has been limited. This systematic review provides insights into material-assisted strategies for OC regeneration, including therapeutic potential and evaluation methods.
Article
Pharmacology & Pharmacy
Rik Oude Egberink, Helen M. Zegelaar, Najoua El Boujnouni, Elly M. M. Versteeg, Willeke F. Daamen, Roland Brock
Summary: In this study, we developed porous collagen scaffolds incorporating peptide-mRNA nanoparticles as off-the-shelf functional biomaterials for regenerative medicine applications. The peptide-mRNA complexes showed uniform distribution in the scaffolds, and the matrices maintained cell attachment and viability. Importantly, the mRNA formulation retained activity after lyophilization and storage at 4 degrees C over two weeks. This research provides a viable alternative to lipid-based mRNA formulations, with better control and lower costs.
Article
Engineering, Biomedical
Steven Vermeulen, Zeinab Tahmasebi Birgani, Pamela Habibovic
Summary: Embryogenic developmental processes involve a tightly controlled regulation between mechanical forces and biochemical cues. Understanding the pathways that govern bone regeneration is crucial for the development of effective regenerative therapies. However, current biochemical stimuli are often insufficient due to the lack of an appropriate mechanical environment. Therefore, further research on biomaterials and their effects on bone regeneration pathways is needed to inspire the development of more effective therapies.
Review
Engineering, Biomedical
Zhi Peng, Heng Sun, Varitsara Bunpetch, Yiwen Koh, Ya Wen, Dongmei Wu, Hongwei Ouyang
Summary: Osteoarthritis (OA) is a leading cause of disability and socioeconomic loss globally, with current pharmacological treatments mainly palliative. Focus is shifting towards targeting cartilage extracellular matrix (ECM) destruction and abnormal homeostasis in OA for regenerative therapies, using potential biomarkers and preclinical studies to identify pathological stages.
Review
Pharmacology & Pharmacy
Noelia Nieto Gonzalez, Antonella Obinu, Giovanna Rassu, Paolo Giunchedi, Elisabetta Gavini
Summary: There is a current lack of formulations suitable for pediatric patients for several drugs, leading to the need for new treatments in pediatric diseases. The application of nanotechnology in pediatric drug formulations has been suggested and studied, primarily focusing on pediatric cancer treatment. While nanomedicine shows promise in addressing pediatric needs, the development of new pediatric medicines based on nanoparticles is hindered by the lack of data on pharmacokinetics, safety, and efficacy.
Review
Medicine, General & Internal
Dan Li, Puneet Gupta, Nicholas A. Sgaglione, Daniel A. Grande
Summary: Osteoarthritis of the knee is a common chronic musculoskeletal condition, and current conservative treatments are unsatisfactory, prompting the need for alternative options such as exosomes. In vivo studies have shown the potential clinical use of exosomes derived from non-classical sources for knee osteoarthritis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
Marco Marcarelli, Marcello Zappia, Lorenzo Rissolio, Chiara Baroni, Carlo Astarita, Letizia Trovato, Antonio Graziano
Summary: Autologous cartilage micrografts can effectively alleviate pain and improve daily activities in patients with knee chondropathy. The treatment leads to significant increases in Oxford Knee Score and visual analogue scale at 6 months post-treatment, with sustained improvement at 3 years. Magnetic resonance imaging shows a noticeable thickening of cartilage after 3 years, indicating the formation of new cartilage.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Chemistry, Multidisciplinary
Yuwen Wang, Andy Tay
Summary: This review discusses the nano-scale properties of nanomaterials, focusing on nanoparticles, and their impact on nanomedicine. It explores the potential of manipulating the chirality of nanomaterials to enhance biomolecular interactions and improve nanotherapeutics. The review also examines the synthesis, characterization, and applications of chiral nanoparticles, particularly in cancer, immunomodulation, and neurodegenerative diseases, and proposes possible mechanisms for the interaction between chiral nanomaterials and cells for biological manipulation. This timely reminder emphasizes the importance of nanoscale modifications in advancing nanotherapeutics research.
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Oystein Ovrebo, Giuseppe Perale, Jonathan P. Wojciechowski, Cecile Echalier, Jonathan R. T. Jeffers, Molly M. Stevens, Havard J. Haugen, Filippo Rossi
Summary: Musculoskeletal defects are a significant healthcare burden, particularly as the population ages. Hydrogels have emerged as a promising solution, with their high biocompatibility and potential for minimally invasive delivery. This review explores the design and clinical translation of hydrogels, with a focus on treatments for cartilage, bone, and the nucleus pulposus. The importance of acquiring high-quality pre-clinical and clinical data for successful translation is emphasized.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Editorial Material
Engineering, Biomedical
Khoon S. Lim, Hala Zreiqat, Debby Gawlitta
ACTA BIOMATERIALIA
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Andrew C. Daly, Khoon S. Lim
Summary: Lithography bioprinting enables the fabrication of high-resolution constructs for potential use in tissue engineering. The seminal work by Grigoryan and colleagues developed bioresins that allow precise control over the x, y, and z-planes in lithography bioprinting, and applied this technique to fabricate physiologically biomimetic alveolar lung models.
TRENDS IN BIOTECHNOLOGY
(2023)
Editorial Material
Engineering, Biomedical
Riccardo Levato, Khoon S. Lim
Summary: The integration of light-driven technologies into biofabrication has revolutionized the field of tissue engineering and regenerative medicine, with great breakthroughs in recent years. Light-based bioprinting approaches have shown the potential to fabricate high-resolution tissue constructs, addressing key challenges in regenerative medicine. Light has also been proven as a powerful tool, leading to novel photo-chemistry and spatial-temporal control over cellular functions. This Special Issue aims to discuss the latest trends and strategies in using light-based technologies in biofabrication, including in vitro models, novel biofabrication technologies, and photo-responsive bioinks.
Article
Engineering, Biomedical
Naveen Vijayan Mekhileri, Gretel Major, Khoon Lim, Isha Mutreja, Kenny Chitcholtan, Elisabeth Phillips, Gary Hooper, Tim Woodfield
Summary: In order to improve the drug discovery process, there is a need for preclinical models that can mimic the complexity and scale of native tumors. This study compares two methods for creating 3D tumor microenvironments, hydrogel microspheres and cell-dense spheroids, which are then assembled into 3D printed scaffolds to form tumor-scale models. The efficiency, cell architecture, and drug response profiles of these models are characterized, and it is found that only cocultured spheroids are suitable for bioassembly. The bioassembly system demonstrates high efficiency and customization, making it valuable for studying tumorigenesis and advancing drug discovery.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Zhen Zhang, Yi Liu, Xuelian Tao, Ping Du, Myagmartsend Enkhbat, Khoon S. Lim, Huaiyu Wang, Peng-Yuan Wang
Summary: This study utilized colloidal self-assembled patterns (cSAPs) to prepare cell co-spheroids of human bone mesenchymal stem cells/human umbilical vein endothelial cells (HBMSCs/HUVECs), which were encapsulated in phenol-modified gelatin/hyaluronan (Gel-Ph/HA-Ph) hydrogels and photo-crosslinked using blue light. The Gel-Ph/HA-Ph hydrogels with a 5%-to-0.3% ratio exhibited the best properties. The HBMSC/HUVEC co-spheroids showed enhanced osteogenic differentiation and vascular network formation, and performed better in angiogenesis and blood vessel development in a subcutaneous nude mouse model compared to HBMSC spheroids.
Article
Chemistry, Multidisciplinary
Dina Abdelmoneim, Gemma Porter, Warwick Duncan, Khoon Lim, Richard Easingwood, Tim Woodfield, Dawn Coates
Summary: This study evaluates the encapsulation and stabilization of silver nanoparticles (AgNPs) in HyStem(R)-C and photo-cross-linked GelMA hydrogels. The results show that even distribution of AgNPs in GelMA significantly decreases cell viability, while AgNP aggregates in HyStem((R))-C do not induce cytotoxicity. The release rate of AgNPs from GelMA after 48 hours is 20% w/w for 0.1μg/mL and 51% for 100μg/mL. GelMA-incorporated AgNPs exhibit antibacterial effects against Escherichia coli and Staphylococcus aureus.
Review
Chemistry, Multidisciplinary
Haoran Wang, Qiyao Li, Parvej Alam, Haotian Bai, Vandana Bhalla, Martin R. Bryce, Mingyue Cao, Chao Chen, Sijie Chen, Xirui Chen, Yuncong Chen, Zhijun Chen, Dongfeng Dang, Dan Ding, Siyang Ding, Yanhong Duo, Meng Gao, Wei He, Xuewen He, Xuechuan Hong, Yuning Hong, Jing-Jing Hu, Rong Hu, Xiaolin Huang, Tony D. James, Xingyu Jiang, Gen-ichi Konishi, Ryan T. K. Kwok, Jacky W. Y. Lam, Chunbin Li, Haidong Li, Kai Li, Nan Li, Wei-Jian Li, Ying Li, Xing-Jie Liang, Yongye Liang, Bin Liu, Guozhen Liu, Xingang Liu, Xiaoding Lou, Xin-Yue Lou, Liang Luo, Paul R. McGonigal, Zong-Wan Mao, Guangle Niu, Tze Cin Owyong, Andrea Pucci, Jun Qian, Anjun Qin, Zijie Qiu, Andrey L. Rogach, Bo Situ, Kazuo Tanaka, Youhong Tang, Bingnan Wang, Dong Wang, Jianguo Wang, Wei Wang, Wen-Xiong Wang, Wen-Jin Wang, Xinyuan Wang, Yi-Feng Wang, Shuizhu Wu, Yifan Wu, Yonghua Xiong, Ruohan Xu, Chenxu Yan, Saisai Yan, Hai-Bo Yang, Lin-Lin Yang, Mingwang Yang, Ying-Wei Yang, Juyoung Yoon, Shuang-Quan Zang, Jiangjiang Zhang, Pengfei Zhang, Tianfu Zhang, Xin Zhang, Xin Zhang, Na Zhao, Zheng Zhao, Jie Zheng, Lei Zheng, Zheng Zheng, Ming-Qiang Zhu, Wei-Hong Zhu, Hang Zou, Ben Zhong Tang
Summary: This review provides a comprehensive summary of the applications of aggregation-induced emission (AIE) luminogens in biological imaging, dynamic physiological processes, disease diagnosis and treatment, and specific analyte detection and monitoring. The review also discusses critical issues and future directions, aiming to promote the development of AIE in the fields of life and health.
Article
Engineering, Biomedical
Alessandro Cianciosi, Sabrina Stecher, Maxi Loeffler, Petra Bauer-Kreisel, Khoon S. Lim, Tim B. F. Woodfield, Juergen Groll, Torsten Blunk, Tomasz Jungst
Summary: This study introduces a soft photoclick resin that enables the fast production of complex cell-laden structures through volumetric bioprinting. The resin is suitable for the high-throughput fabrication of constructs containing adipose-derived stromal cells and differentiated adipocytes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Gretel Major, Alessia Longoni, Jeremy Simcock, Nicholas J. Magon, Jessica Harte, Boushra Bathish, Roslyn Kemp, Tim Woodfield, Khoon S. Lim
Summary: Visible light-mediated cross-linking is a useful method for enhancing the structural control of laboratory-based polymers. This study evaluated the utility of a ruthenium/sodium persulfate photocross-linking system for improving structural control in patient-derived lipoaspirate for soft tissue reconstruction. The photocross-linking strategy demonstrated the capability of improving structural control and achieving better patient outcomes with minimal manipulation in surgical procedures.
Article
Chemistry, Multidisciplinary
Shixian Liu, Yuting Hou, Zirui Li, Chenyu Yang, Guozhen Liu
Summary: A fully integrated device for salivary detection, which operates in a sample-in-answer-out manner, is crucial for noninvasive point-of-care testing and screening of contagious diseases. This study developed a universal platform by integrating a centrifugal microfluidic disc with microfluidic paper-based analytical devices (mu PADs) for salivary diagnostics. The integrated device demonstrated high efficiency in saliva treatment and was able to detect the SARS-CoV-2 N protein with high specificity and comparable performance to commercial paper lateral flow assay test strips.
Article
Materials Science, Biomaterials
Gretel Major, Jeremy Simcock, Abhishek Kumar, Torsten Kleffmann, Tim B. F. Woodfield, Khoon S. Lim
Summary: This study provides a comprehensive proteomic evaluation of the adipose tissue matrisome, revealing considerable differences in the expression of matrisome proteins across different individuals and factors such as age and lipoaspirate collection technique. The study also highlights the high abundance of basement membrane proteins and fibrillar collagens in adipose tissue, reflecting its hierarchical structure. These findings contribute to a better understanding of adipose-related pathologies and can be further utilized for the development of adipose-based biomaterials.
Article
Materials Science, Biomaterials
Alessia Longoni, Gretel S. Major, Shaoyuan Jiang, Brooke L. Farrugia, David C. Kieser, Tim B. F. Woodfield, Jelena Rnjak-Kovacina, Khoon S. Lim
Summary: Synthetic polymers are widely used in tissue engineering and regenerative medicine, but their bioinert nature limits their interaction with cells and host tissue. This study explores the use of gelatin to enhance the biofunctionality of synthetic hydrogels by promoting cell infiltration and blood vessel recruitment. The results show that gelatin can enhance the angiogenic potential of hydrogels, making them promising for a variety of applications.
BIOMATERIALS SCIENCE
(2023)
Correction
Chemistry, Multidisciplinary
Yan Liu, Guo-Zhen Liu, Xiao-Mao Li, Peng Jiang, Yong-Gang Xia, Hai-Xue Kuang, Bing-You Yang
CHINESE JOURNAL OF CHEMISTRY
(2023)
