Article
Plant Sciences
Jian Bao, Gentu Ge, Zhijun Wang, Yanzi Xiao, Muqier Zhao, Lin Sun, Yu Wang, Jiawei Zhang, Yushan Jia, Shuai Du
Summary: The objective of this study was to isolate LAB from native grasses and naturally fermented silages, determine their identity, and assess their effects on silage quality and bacterial communities. It was found that L. fermentum and L. graminis improved the quality of native grass silage.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Meiling Hou, Zhijun Wang, Lin Sun, Yushan Jia, Shicong Wang, Yimin Cai
Summary: This study investigated the chemical composition, fermentation, and characteristics of lactic acid bacteria (LAB) in silage made from native grass on the Inner Mongolian Plateau. The results showed that the native grass contained abundant LAB species and could be used as high-quality silage. The dominant LAB species isolated were P. acidilactici and E. faecium, which could potentially serve as excellent inoculants for native grass silage.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jiawei Zhang, Yichao Liu, Zhijun Wang, Jian Bao, Muqier Zhao, Qiang Si, Pengbo Sun, Gentu Ge, Yushan Jia
Summary: The addition of lactic acid bacteria can improve the quality of native grass silage and change the bacterial community structure. Lactobacillus plantarum benefits fermentation quality during ensiling, while Lactobacillus buchneri inhibits silage deterioration during aerobic exposure. Compound LAB addition is more beneficial for native grass silage.
Article
Microbiology
Hao Guan, Haiping Li, Li Gan, Shiyong Chen, Yanhong Yan, Zhifeng Jia, Wenhui Liu, Xiaoxing Wei, Xiang Ma, Qingping Zhou
Summary: Mixing grass with legumes before ensiling can improve dry matter and crude protein yield, but more information is needed to balance nutrient content and fermentation quality. This study evaluated the microbial community, fermentation characteristics, and nutrient content of Napier grass mixed with alfalfa at different ratios. Increasing the proportion of alfalfa increased dry matter and crude protein content, and decreased neutral detergent fiber and acid detergent fiber. Inoculation with lactic acid bacteria improved fermentation quality and increased the abundance of Lactiplantibacillus. The M3 and M5 groups achieved the optimal balance of nutrients and fermentation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kathy G. Arriola, Diwakar Vyas, Donghyeon Kim, Mariele C. N. Agarussi, Vanessa P. Silva, Milton Flores, Yun Jiang, Xue Yanlin, Andres A. Pech-Cervantes, Luiz F. Ferraretto, Adegbola T. Adesogan
Summary: The study aimed to determine the effect of inoculation with Lactobacillus hilgardii and Lactobacillus buchneri on sorghum and corn silage. Results showed that inoculating with LB or LH separately improved aerobic stability, while the combination of LB and LH prevented increases in DM losses caused by their separate use.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Microbiology
Natali Hernandez-Parada, Oscar Gonzalez-Rios, Mirna Leonor Suarez-Quiroz, Zorba Josue Hernandez-Estrada, Claudia Yuritzi Figueroa-Hernandez, Juan de Dios Figueroa-Cardenas, Patricia Rayas-Duarte, Maria Cruz Figueroa-Espinoza
Summary: The use of sourdough in bread production involves fermentation dominated by lactic acid bacteria (LAB) and yeast. Different starter cultures from various food sources can be used to initiate sourdough fermentation, enhancing the aroma and flavor profile of bread. However, the influence of microorganisms from different food products and by-products on sourdough fermentation is not well documented. This review focuses on exploring the use of various starter cultures in sourdough fermentation, discussing sourdough types, the fermentation process, and biochemical transformations.
Article
Agriculture, Dairy & Animal Science
Mao Li, Xuejuan Zi, Hanlin Zhou, Renlong Lv, Jun Tang, Yimin Cai
Summary: This study found that LAB and molasses have significant effects on the microbial community and fermentation quality of cassava foliage silage, with the combination showing the most effective results. The addition of LAB and molasses can increase the abundance of Lactobacillus, reduce harmful bacteria, and improve fermentation quality. The bacterial community structure is closely related to fermentation indexes, indicating the importance of microbial diversity in silage fermentation.
ANIMAL SCIENCE JOURNAL
(2021)
Review
Microbiology
Charles Obinwanne Okoye, Yongli Wang, Lu Gao, Yanfang Wu, Xia Li, Jianzhong Sun, Jianxiong Jiang
Summary: Ensiling is a microbial-driven process used to preserve fresh forage in bio-refinery and animal production. Lactic acid bacteria (LAB) species are widely recognized as additives in silage production. However, there is a lack of information on gene expression and molecular mechanisms of microbiota involved in silage production, leading to inconsistent silage quality. Modern research focuses on improving LAB inoculants through advanced biotechnological approaches. This review highlights the role of LAB inoculants in silage production and explores the use of metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation for developing high-performance LAB strains. It also discusses the trends and future perspectives of LAB development for silage improvement in sustainable agriculture.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Microbiology
Charles Obinwanne Okoye, Yongli Wang, Lu Gao, Yanfang Wu, Xia Li, Jianzhong Sun, Jianxiong Jiang
Summary: This study discusses the role of LAB inoculants in forage production and modern biotechnology approaches such as metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation as powerful tools for identifying, improving, and developing high-performance LAB strains. Furthermore, the review highlights the trends and future perspectives of LAB development for forage improvement, essential for breakthroughs in sustainable agriculture.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Wei Wang, Yangyi Hao, Chenglong Luo, Qianqian Wang, Zhonghan Wang, Dong Li, Jing Yuan, Zhijun Cao, Hongjian Yang, Shengli Li
Summary: This study aimed to explore the effects of different silage additives on the chemical composition, fermentation profile, as well as the in vitro and in situ digestibility of paper mulberry (PM) silage. The results showed that the addition of LAB, MOL, and their combination can improve PM silage fermentation and improve the in situ digestibility of dry matter and acid detergent fiber.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Shuai Du, Sihan You, Xiaowei Jiang, Yuyu Li, Ruifeng Wang, Gentu Ge, Yushan Jia
Summary: Bioaugmentation with different lactic acid bacteria improved the ensiling performance of native grass silage by reducing water-soluble carbohydrate content, increasing crude protein content, and decreasing fiber contents. LAB inoculation also led to a decrease in pH and an increase in lactic acid and acetic acid contents. The bacterial community composition changed after ensiling. These findings suggest the potential of LAB inoculation in improving the quality of native grass silage.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Z. Du, L. Sun, Y. Lin, C. Chen, F. Yang, Y. Cai
Summary: This study investigates the preparation and effects of PM silages using locally available woody forage and hay resources. The results show that PM silages prepared with different proportions of hay have improved fermentation quality, with Lactobacillus plantarum becoming the dominant bacterial community and changes in the proportions of related metabolic pathways.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Agronomy
Ekaterina Anokhina, Svetlana Obraztsova, Anna Tolkacheva, Dmitrii Cherenkov, Tatyana Sviridova, Olga Korneeva
Summary: The use of amaranth green mass as an alternative source of protein and fiber for canned feed for ruminants is very promising. A developed silage starter from lactic acid bacteria significantly improves the quality of amaranth silage by promoting rapid fermentation and increasing crude protein retention.
Review
Food Science & Technology
Dharmendra Kumar, Milan Kumar Lal, Som Dutt, Pinky Raigond, Sushil Sudhakar Changan, Rahul Kumar Tiwari, Kumar Nishant Chourasia, Vikas Mangal, Brajesh Singh
Summary: The current trend of health-conscious consumers and healthy food habits has led researchers to explore the development of food products with synbiotic benefits. This review article explores the alternative sources of probiotics and highlights various methods for making non-dairy synbiotics based on dietary fibers.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Thomas Eliasson, Li Sun, Ase Lundh, Annika Hojer, Karin Hallin Saeden, Marten Hetta, Horacio Gonda
Summary: This study aims to investigate the epiphytic microbiota in grass-clover herbage harvested at different sites and occasions, as well as explore the effects of different silage additives on the resulting microbiota. The study found that the microbiota in the herbage was random and not dependent on site or harvesting time. Silage additives had a major impact on the ensiling process, with inoculation resulting in well-fermented silages dominated by Lactobacillus and Pediococcus genera. Acid-treatment surprisingly resulted in a less preferable silage outcome. Silage making without additives is not recommended based on the study's results.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Junfeng Hao, Yuyu Li, Yushan Jia, Zhijun Wang, Rong Rong, Jian Bao, Muqier Zhao, Zhihui Fu, Gentu Ge
Summary: The major flavonoids in L. indica L. cv. Mengzao include rutin, luteolin, and others, with the highest content found in flowers and leaves. The flowering period is the most suitable time for harvesting this medicinal plant.
Article
Agronomy
Ning Yuan, Lin Sun, Shuai Du, Gentu Ge, Zhijun Wang, Yuyu Li, Jian Bao, Muqier Zhao, Qiang Si, Junfeng Hao, Yushan Jia
Summary: Harvest time and storage time have significant effects on nutrient quality and Volatile Organic Compounds (VOCs) of alfalfa. The best harvest time for alfalfa is during the budding stage, as it has the highest nutrient quality and desired VOC composition. Long-term storage can lead to increased levels of certain compounds and changes in the smell of fresh alfalfa.
Article
Microbiology
Zhihui Fu, Lin Sun, Meiling Hou, Junfeng Hao, Qiang Lu, Tingyu Liu, Xiuzhen Ren, Yushan Jia, ZhiJun Wang, Gentu Ge
Summary: This study investigated the effects of different harvest frequencies on the fermentation quality, microbial community, and metabolites of ryegrass silage. The results showed that harvest frequency significantly influenced the pH and lactic acid content of the silage, as well as the composition of the microbial community. Various differential metabolites were identified, with peptides being the most annotated metabolites.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Zhihui Fu, Lin Sun, Zhijun Wang, Jingyi Liu, Meiling Hou, Qiang Lu, Junfeng Hao, Yushan Jia, Gentu Ge
Summary: This study investigated the effects of different growth stages on the fermentation quality, microbial community, metabolic pathways, and metabolomic characteristics of Italian ryegrass silage. The results showed that the booting period had the lowest pH and the highest lactic acid content. Lactiplantibacillus plantarum, Weissella minor, and Levilactobacillus brevis were the dominant bacteria in each group. The study also identified the enriched metabolic pathways and correlations between bacteria and metabolites. Overall, this study provides insights into the interaction mechanisms during ensiling fermentation and lays a foundation for producing safe and high-quality silage.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Muqier Zhao, Zhijun Wang, Shuai Du, Lin Sun, Jian Bao, Junfeng Hao, Gentu Ge
Summary: The objective of this study was to investigate the effects of Lactobacillus plantarum and propionic acid on the microbial community and fermentation performance of high-moisture amaranth silage. The results showed that the addition of L. plantarum or propionic acid improved the fermentation quality by increasing the content of lactic acid and lactic acid bacteria, and reducing the pH and the counts of undesirable microorganisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Zhihui Fu, Lin Sun, ZhiJun Wang, Yichao Liu, Junfeng Hao, Cuiping Gao, Gentu Ge
Summary: This study aimed to evaluate the effects of different regions in northern China on the fermentative products, bacterial community compositions, and metabolic pathways and metabolites of Italian ryegrass silage. The SYK group had the lowest pH, highest lactic acid contents, and largest lactic acid bacteria populations among the treatment groups. Lactiplantibacillus plantarum was found to be positively correlated with various metabolites.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Junfeng Hao, Qiang Si, Zhijun Wang, Yushan Jia, Zhihui Fu, Muqier Zhao, Andreas Wilkes, Gentu Ge
Summary: The triterpenoids in the leaves of Lactuca indica L.cv. Mengzao were extracted using microwave-assisted ethanol extraction. The optimal extraction conditions were determined and the effects of various factors on the triterpenoids content were evaluated. The study found that the leaves had the highest triterpenoids content, with the flowering stage being the best time for extraction. The triterpenoids exhibited strong antioxidant activity against DPPH and ABTS free radicals. The results provide a valuable reference for the development of intensive processing methods for L. indica.