Article
Cell Biology
Alexia Tasca, Martin Helmstaedter, Magdalena Maria Brislinger, Maximilian Haas, Brian Mitchell, Peter Walentek
Summary: The study found that multiciliated cells can undergo apoptosis or trans-differentiate into goblet secretory cells during developmental tissue remodeling, both processes dependent on Notch signaling and modulated by other signaling pathways.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Carlos F. H. Serra, Helu Liu, Jun Qian, Munemasa Mori, Jining Lu, Wellington Cardoso
Summary: Differences in ciliary morphology and dynamics among multiciliated cells of the respiratory tract contribute to efficient mucociliary clearance. This study reveals that Prom1 plays a crucial role in establishing these phenotypic differences. Prom1 is induced by Notch in multiciliated cells, and its expression shows a gradient along the proximal-distal axis of the airways. Prom1 is not required for multiciliated formation, but its inactivation leads to longer cilia that beat at a lower frequency. Notch fine-tunes Prom1 levels to regulate the multiciliated cell phenotype and generate diversity among these cells, which could help protect the lung and prevent pulmonary disease.
Article
Cell Biology
Michael Lewis, Berta Terre, Philip A. Knobel, Tao Cheng, Hao Lu, Camille Stephan-Otto Attolini, Jordann Smak, Etienne Coyaud, Isabel Garcia-Cao, Shalu Sharma, Chithran Vineethakumari, Jessica Querol, Gabriel Gil-Gomez, Gabriele Piergiovanni, Vincenzo Costanzo, Sandra Peiro, Brian Raught, Haotian Zhao, Xavier Salvatella, Sudipto Roy, Moe R. Mahjoub, Travis H. Stracker
Summary: Multiciliated cells (MCCs) promote fluid or gamete movement in mammalian organs through the projection of motile cilia from their apical surface. The transcriptional activators GEMC1 and MCIDAS interact with E2F4/5-DP1 and play a crucial role in MCC differentiation. The engagement of distinct SWI/SNF subcomplexes by GEMC1 and MCIDAS is necessary for MCC-specific transcriptional regulation.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Guilherme Ventura, Aboutaleb Amiri, Raghavan Thiagarajan, Mari Tolonen, Amin Doostmohammadi, Jakub Sedzinski
Summary: During embryonic development, cells migrate and integrate into target tissues to execute their function. This research reveals that multiciliated cell precursors in Xenopus embryo use filopodia to sense vertex stiffness and remodel epithelial junctions of neighboring cells, facilitating integration.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Cait E. Hamele, Alistair B. Russell, Nicholas S. Heaton
Summary: Influenza A virus is a significant threat to public health, but why some individuals clear the infection asymptomatically while others become severely ill remains unclear. This study used single-cell RNA sequencing to explore the range of responses in susceptible epithelial cells during an acute influenza A virus infection. It was found that different preexisting gene expression patterns in the initial target cells may ultimately affect the establishment of viral disease.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Chanjae Lee, Yun Ma, Fan Tu, John B. Wallingford
Summary: The coordinated action of various machineries, including axonemal dynein arms, central apparatus, radial spokes, and microtubule inner proteins, is crucial for the beating of motile cilia. However, little is known about their interplay during motile ciliogenesis.
Article
Orthopedics
Cara E. Felter, Jonathan Cicone, Lindsey Mathis, Deanna L. Smith
Summary: The COVID-19 pandemic has exacerbated health disparities for communities of color and those with limited socioeconomic means due to social determinants of health. These disparities also affect students' learning environments and abilities, leading to higher academic risks during the educational process.
Article
Biology
Gina M. LoMastro, Chelsea G. Drown, Aubrey L. Maryniak, Cayla E. Jewett, Margaret A. Strong, Andrew Jon Holland
Summary: Multiciliated cells assemble multiple motile cilia through centriole amplification and surface area expansion. Recent research has shown that Polo-like kinase 4 plays a critical role in centriole amplification in multiciliated cells.
Article
Multidisciplinary Sciences
Ansley S. Conchola, Tristan Frum, Zhiwei Xiao, Peggy P. Hsu, Kamika Kaur, Michael S. Downey, Renee F. C. Hein, Alyssa J. Miller, Yu- Hwai Tsai, Angeline Wu, Emily M. Holloway, Abhinav Anand, Preetish Kadur Lakshminarasimha Murthy, Ian Glass, Purushothama R. Tata, Jason R. Spence
Summary: Using scRNA-seq and microscopy, a cell enriched in the lower airways of developing human lung was identified by the coexpression of SCGB3A2/SFTPB/CFTR. By applying the CellTagging single-cell barcode-based lineage tracing method, the fate of SCGB3A2/SFTPB/CFTR cells during airway organoid differentiation was tracked, revealing their distinct differentiation potential and contribution to epithelial diversity in this region. These findings were supported by studies using organoids and isolated cells from the lower noncartilaginous airway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Saurabh Kulkarni, Jonathan Marquez, Priya Date, Rosa Ventrella, Brian J. Mitchell, Mustafa K. Khokha
Summary: Cells regulate the number of cilia and centrioles in multiciliated cells (MCCs) through mechanical forces, centrioles, and the mechanosensitive ion channel Piezo1, ensuring optimal cell function. This study provides new insights into organelle number control in cells.
Article
Multidisciplinary Sciences
Remy Robinot, Mathieu Hubert, Guilherme Dias de Melo, Francoise Lazarini, Timothee Bruel, Nikaia Smith, Sylvain Levallois, Florence Larrous, Julien Fernandes, Stacy Gellenoncourt, Stephane Rigaud, Olivier Gorgette, Catherine Thouvenot, Celine Trebeau, Adeline Mallet, Guillaume Dumenil, Samy Gobaa, Raphael Etournay, Pierre-Marie Lledo, Marc Lecuit, Herve Bourhy, Darragh Duffy, Vincent Michel, Olivier Schwartz, Lisa A. Chakrabarti
Summary: Understanding how SARS-CoV-2 spreads within the respiratory tract is crucial in determining the severity of COVID-19. This study shows that SARS-CoV-2 infection leads to rapid loss of airway motile cilia, affecting cilia clearance function, with reduced expression of the ciliogenesis regulator Foxj1 preceding cilia loss. Further analysis in Syrian hamsters confirms the loss of motile cilia in vivo.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Clara Maria Mateos-Quiros, Sergio Garrido-Jimenez, Guadalupe Alvarez-Hernan, Selene Diaz-Chamorro, Juan Francisco Barrera-Lopez, Javier Francisco-Morcillo, Angel Carlos Roman, Francisco Centeno, Jose Maria Carvajal-Gonzalez
Summary: The expression of junctional adhesion molecule 3 (Jam3) in multiciliated cells (MCCs) in the airway epithelium varies during differentiation of airway basal stem cells (BSCs) and in response to certain treatments, but it is not essential for BSC differentiation into specific cell types. Jam3 in MCC is mostly localized to subapical organelles, suggesting a role unrelated to its known barrier function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ani Kardashian, Julius Wilder, Norah A. Terrault, Jennifer C. Price
Summary: The COVID-19 pandemic has revealed healthcare inequities in the USA and how social determinants of health impact disparities in liver disease. Closing the gap in health equity in hepatology involves addressing social determinants at the individual, community, and societal levels through interventions such as systematic screening, partnerships with public health workers, and advocacy for policy reform.
Article
Developmental Biology
Sun K. Kim, Eva Brotslaw, Virginie Thome, Jen Mitchell, Rosa Ventrella, Caitlin Collins, Brian Mitchell
Summary: Centriole amplification in multiciliated cells is regulated by a process involving the deuterosome, with the centrosomal protein Cep70 identified as a novel regulator. Cep70 associates with deuterosomes during centriole biogenesis, and depletion of Cep70 leads to a decrease in centriole formation and defects in radial intercalation in multiciliated cells. This suggests that Cep70 plays a crucial role in regulating centriole biogenesis in multiciliated cells.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Microscopy
Keishi Narita, Sen Takeda
Summary: Researchers found unusual cilia-related structures in the ependyma of wild-type mouse, revealing some unique features during multiciliogenesis. Five percent of cilia existed as bundles, and apical protrusions containing multiple sets of axonemes were occasionally observed.
Article
Cell Biology
Neophytos Christodoulou, Paris A. Skourides
Article
Multidisciplinary Sciences
Sarah Ellys Harrison, Berna Sozen, Neophytos Christodoulou, Christos Kyprianou, Magdalena Zernicka-Goetz
Article
Developmental Biology
Sara Zanardelli, Neophytos Christodoulou, Paris A. Skourides
DEVELOPMENTAL BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Nicoletta I. Petridou, Panayiota Stylianou, Neophytos Christodoulou, Daniel Rhoads, Jun-Lin Guan, Paris A. Skourides
Article
Biotechnology & Applied Microbiology
Anna Charalambous, Ioanna Antoniades, Neophytos Christodoulou, Paris A. Skourides
JOURNAL OF NANOBIOTECHNOLOGY
(2011)
Correction
Cell Biology
Neophytos Christodoulou, Christos Kyprianou, Antonia Weberling, Ran Wang, Guizhong Cui, Guangdun Peng, Naihe Jing, Magdalena Zernicka-Goetz
NATURE CELL BIOLOGY
(2018)
Article
Cell Biology
Neophytos Christodoulou, Christos Kyprianou, Antonia Weberling, Ran Wang, Guizhong Cui, Guangdum Peng, Naihe Jing, Magdalena Zernicka-Goetz
NATURE CELL BIOLOGY
(2018)
Article
Biology
K. Kaouri, P. K. Maini, P. A. Skourides, N. Christodoulou, S. J. Chapman
JOURNAL OF MATHEMATICAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Neophytos Christodoulou, Antonia Weberling, Douglas Strathdee, Kurt Anderson, Paul Timpson, Magdalena Zernicka-Goetz
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Christos Kyprianou, Neophytos Christodoulou, Russell S. Hamilton, Wallis Nahaboo, Diana Suarez Boomgaard, Gianluca Amadei, Isabelle Migeotte, Magdalena Zernicka-Goetz
Article
Developmental Biology
Neophytos Christodoulou, Paris A. Skourides
Summary: Research has found that neural tube closure is a crucial step in vertebrate development, involving convergent extension and apical constriction. This process occurs in two stages, with each stage having different spatiotemporal contributions. Additionally, the movement of the surface ectoderm is found to be associated with neural plate morphogenesis, and excessive resistive forces from the surface ectoderm negatively impact neural plate morphogenesis.
Article
Cell Biology
Neophytos Christodoulou, Paris A. Skourides
Summary: Neural tube closure is a crucial process in vertebrate embryogenesis, and defects in this process can lead to common birth defects. This study investigates the mechanical communication between the neural plate and the somitic mesoderm and its impact on tissue morphogenesis using Xenopus laevis embryos.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)