Article
Agriculture, Dairy & Animal Science
Shenyan Gao, Huayuan Chen, Xiaozhan Zhang, Jun Zhao, Zeng Wang
Summary: This study identified cellular proteins that interact with the 100K protein of hypervirulent FAdV-4 and found that they positively regulate viral replication. These findings are important for understanding the pathogenic mechanisms of FAdV-4.
Review
Virology
Shiyu Dai, Fei Deng, Hualin Wang, Yunjia Ning
Summary: Crimean-Congo hemorrhagic fever virus is a tick-borne pathogen that causes severe illness and has limited treatment options without FDA-approved vaccines or specific antiviral medications. The risk of transmission has increased due to climate change and globalization, highlighting the need for greater efforts in finding therapeutic approaches.
Article
Microbiology
Fuli Ren, Shu Shen, Yun-Jia Ning, Qiongya Wang, Shiyu Dai, Junming Shi, Min Zhou, Hualin Wang, Chaolin Huang, Ding-Yu Zhang, Fei Deng
Summary: SFTSV NSs are involved in regulating virus-like or viral RNA synthesis by interacting with N, inhibiting virus-like RNA or virus replication; the negative effect may be attributed to the interaction between NSs and N, which is closely correlated with the IB structure formed by NSs.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Taif Shah, Qian Li, Binghui Wang, Zulqarnain Baloch, Xueshan Xia
Summary: Ticks are arthropods that transmit infectious pathogens to humans and animals. Certain tick species can transmit viruses such as Bourbon virus, Dhori virus, Powassan virus, Omsk hemorrhagic fever virus, Colorado tick fever virus, Crimean-Congo hemorrhagic fever virus, Heartland virus, and Kyasanur forest disease virus. Understanding the eco-epidemiology and pathogenesis of these tick-borne viruses is vital for effective prevention. This review provides an overview of medically important ticks and tick-borne viruses, including their epidemiology, pathogenesis, and disease manifestations.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jing Wu, Yuxue Zhao, Qiaoqiao Chen, Yiwen Chen, Jiaqi Gu, Lingxiang Mao
Summary: This study found that EV-A71 infection can increase exosome secretion, and the nonstructural protein 3A can promote exosome secretion. The interaction between 3A and the small GTPase protein Rab27a can protect Rab27a from ubiquitination, resulting in increased exosome release.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
David W. Hawman, Kimberly Meade-White, Shanna Leventhal, Friederike Feldmann, Atsushi Okumura, Brian Smith, Dana Scott, Heinz Feldmann
Summary: Crimean-Congo hemorrhagic fever (CCHF) is a severe disease caused by the CCHFV virus with a fatality rate as high as 30%. Our understanding of the pathogenesis is limited due to the lack of suitable animal models. A mouse-adapted variant of CCHFV has been developed, allowing for better study of host and viral determinants in a mouse model.
Article
Biochemistry & Molecular Biology
Elizaveta Starodubova, Ksenia Tuchynskaya, Yulia Kuzmenko, Anastasia Latanova, Vera Tutyaeva, Vadim Karpov, Galina Karganova
Summary: Tick-borne encephalitis (TBE) is a zoonotic disease that can cause severe neurological complications or death. In this study, the nonstructural protein 1 (NS1) of the tick-borne encephalitis virus (TBEV) was investigated as a genetic determinant of TBE pathogenicity. The NS1 proteins of three TBEV strains belonging to different subtypes were found to have varying levels of accumulation and secretion in mouse cells. The NS1 proteins were also able to induce cytokine production and the profile of cytokines differed between the strains. These findings highlight the role of NS1 diversity in TBEV pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Jielin Tang, Chonghui Xu, Muqing Fu, Canyu Liu, Xianwen Zhang, Wei Zhang, Rongjuan Pei, Yun Wang, Yuan Zhou, Jizheng Chen, Zhichao Miao, Guangjin Pan, Qi Yang, Xinwen Chen
Summary: This study reveals that mammalian ste20-like kinase 3 (MST3) is involved in the regulation of tick-borne encephalitis virus (TBEV) infection. Knockdown or knockout of MST3 inhibits TBEV replication, while other mammalian ste20-like kinase family members do not have this effect. MST3 interacts with the viral proteins NS2A and prM, enhancing the interaction of NS2A-NS4A and mediating virion assembly.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Nidhi Chaudhary, Shikha Srivastava, Upma Dave, Amrita Ojha, Prasenjit Guchhait, Anmol Chandele, Ashok Kumar Patel
Summary: This study reveals that Dengue virus type 2 (DENV-2) induces cytoplasmic translocation and secretion of HMGB1, which further stimulates the production of proinflammatory cytokines. Silencing of HMGB1 inhibits viral replication in DENV-2 infected cells, while inhibition of HMGB1 secretion enhances viral propagation. These findings highlight the importance of HMGB1 in DENV infection and suggest it as a potential target for antiviral therapy.
Article
Biochemistry & Molecular Biology
Sambor Grygorczuk, Justyna Dunaj-Malyszko, Piotr Czupryna, Artur Sulik, Kacper Toczylowski, Agnieszka Siemieniako-Werszko, Agnieszka Zebrowska, Slawomir Pancewicz, Anna Moniuszko-Malinowska
Summary: This study found that the detection of TBEV RNA in plasma may have diagnostic significance in the peripheral phase of TBE. The absence of TBEV RNA in the cerebrospinal fluid suggests a relatively low intrathecal viral burden.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Chunhui Song, Hanze Liu, Zhi Cao, Hu Shan, Qiaoya Zhang
Summary: Heat shock protein 27 (HSP27) is induced and phosphorylated during porcine reproductive and respiratory syndrome virus (PRRSV) infection. Overexpression of HSP27 promotes PRRSV replication, while its knockdown reduces PRRSV proliferation. The phosphorylation of HSP27 is involved in its interaction with viral nonstructural proteins (nsps). In conclusion, HSP27 plays a crucial role in PRRSV replication.
Editorial Material
Medicine, General & Internal
Carmen Adella Sirbu, Constantin Stefani, Marian Mitrica, Gabriela Simona Toma, Aurelian Emil Ranetti, Any Docu-Axelerad, Aida Mihaela Manole, Ion Stefan
Summary: Some neurotropic viruses can cause specific lesions in deep structures, such as the basal ganglia and thalamus, leading to different types of epileptic seizures. This case report describes a 25-year-old male who presented with altered consciousness, agitation, and seizures after traveling to Turkey and Egypt. The diagnosis of tick-borne encephalitis was supported by cerebrospinal fluid analysis, electroencephalography, magnetic resonance imaging showing hyper signal in the basal ganglia, and IgM antibodies. The patient's seizures improved over time and the MRI images returned to almost normal, without seizure recurrence after reducing antiepileptic doses.
Article
Plant Sciences
Jianbin Chen, Limin Zheng, Xiaobin Shi, Songbai Zhang, Xinqiu Tan, Xingyue Zhao, Bingxin Lu, Qian Ye, Shuyue Miao, Yong Liu, Deyong Zhang
Summary: In this study, the NSs protein encoded by tomato zonate spot virus (TZSV) was identified as a potent viral suppressor of RNA silencing (VSR) and was shown to enhance the pathogenicity of potato virus X (PVX) symptoms in Nicotiana benthamiana. The NSs protein was found to interact with the suppressor of gene silencing 3 protein (NbSGS3), a component of the plant RNA silencing pathway, and this interaction was associated with the VSR activity of TZSV NSs. The study reveals a novel molecular mechanism of NSs-mediated suppression of plant host antiviral defense.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Virology
Kuan Feng, Huijiao Zhang, Zhenyu Jiang, Min Zhou, Yuan-Qin Min, Fei Deng, Peiqing Li, Hualin Wang, Yun-Jia Ning
Summary: In addition to its role in maintaining cellular homeostasis, autophagy is now recognized as a crucial player in host defense against various infections. This study reveals a new viral mechanism of manipulating autophagy for its own benefit, using SFTSV as a model. The nonstructural protein of SFTSV interacts with mTOR, the key regulator of autophagy, and impairs its negative regulation on ULK1, leading to autophagy induction.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Veronika J. M. Breitkopf, Gerhard Dobler, Peter Claus, Hassan Y. Naim, Imke Steffen
Summary: Tick-borne flaviviruses (TBFV) can enhance protein and lipid biosynthesis by activating the IRE1 pathway, with the neurotropic TBEV strain Neudoerfl showing strong upregulation of XBP1 in all cell types, but with faster kinetics in central nervous system cells.
Article
Immunology
Ye Hong, Maosheng Bai, Xian Qi, Chuan Li, Mifang Liang, Dexin Li, Carol J. Cardona, Zheng Xing
JOURNAL OF IMMUNOLOGY
(2019)
Article
Infectious Diseases
Zhifeng Li, Changjun Bao, Jianli Hu, Chengfeng Gao, Nan Zhang, Huo Xiang, Carol J. Cardona, Zheng Xing
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Biochemistry & Molecular Biology
Jing Wu, Weiwei Tao, Dandan Bu, Yue Zhao, Tongyu Zhang, Danyang Chong, Bin Xue, Zheng Xing, Chaojun Li
Article
Multidisciplinary Sciences
Bingqian Qu, Xue Li, Carol J. Cardona, Zheng Xing
Article
Multidisciplinary Sciences
Jianguang Fu, Changjun Bao, Xiang Huo, Jianli Hu, Chao Shi, Qin Lin, Jun Zhang, Jing Ai, Zheng Xing
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Menghuai Sun, Kunlong Yan, Chunyang Wang, Jiao Xing, Zhaojun Duan, Yu Jin, Carol J. Cardona, Zheng Xing
Summary: The study revealed that human tonsillar epithelial cells infected with EV-A71 undergo apoptosis through the mitochondria-mediated intrinsic apoptotic pathway. In addition, the infection induces the production of interferon, pro-inflammatory cytokines, and chemokines. These findings contribute to a better understanding of the mechanism of EV-A71 infection and host response.
Article
Virology
Chengfeng Gao, Chunxia Wen, Zhifeng Li, Shuhan Lin, Shu Gao, Haida Ding, Peng Zou, Zheng Xing, Yufeng Yu
Summary: This study demonstrates that fludarabine effectively inhibits RNA virus infections with low cytotoxicity, suggesting its potential development as a broad-spectrum anti-RNA virus therapeutic agent.
Article
Immunology
Chunxia Wen, Yufeng Yu, Chengfeng Gao, Xian Qi, Carol J. Cardona, Zheng Xing
Summary: Apoptosis, pyroptosis, and necroptosis are regulated cell death processes that can impact the outcome of viral diseases in hosts. This study found that human astrocytes infected with ZIKV underwent necroptosis, as evidenced by increased phosphorylation of RIPK1, RIPK3, and MLKL proteins. Inhibition of RIPK3, but not RIPK1, suppressed cell death, indicating RIPK1-independence in ZIKV-induced necroptosis. The findings suggest that necroptosis may play a protective role against viral replication in astrocytes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Jianguang Fu, Jing Ai, Changjun Bao, Jun Zhang, Qingbin Wu, Liguo Zhu, Jianli Hu, Zheng Xing
Summary: This study conducted a molecular epidemiological analysis on the prevalent GII.3[P12] noroviruses in Jiangsu between 2010 and 2019, revealing the evolutionary characteristics of this virus strain in China. The results suggested that evolution may be driven by intergenic recombination and mutations in key amino acid sites.
Article
Pharmacology & Pharmacy
Chengfeng Gao, Yufeng Yu, Chunxia Wen, Zhifeng Li, Menghuai Sun, Shu Gao, Shuhan Lin, Shenjiao Wang, Peng Zou, Zheng Xing
Summary: Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by a novel phlebovirus (SFTSV) with high mortality. Peptides SGc1 and SGc8 derived from the SFTSV glycoprotein Gc have shown potential as specific antiviral therapeutics by inhibiting SFTSV replication effectively.
ANTIVIRAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Zhifeng Li, Jianli Hu, Changjun Bao, Chengfeng Gao, Nan Zhang, Carol J. Cardona, Zheng Xing
Summary: The activation of the NLRP3 inflammasome and induction of pyroptosis, leading to increased secretion of IL-1 beta/IL-18, play important roles in viral pathogenesis and host protection during severe fever with thrombocytopenia syndrome virus (SFTSV) infection. Pyroptosis, as part of innate immunity, might be essential in proinflammatory responses and pathogenicity in humans infected with this novel phlebovirus.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Menghuai Sun, Qian Lin, Chunyang Wang, Jiao Xing, Kunlong Yan, Zhifeng Liu, Yu Jin, Carol J. Cardona, Zheng Xing
Summary: The EV-A71 2B protein plays a crucial role in dysregulating JAK-STAT signaling by promoting caspase-3 dependent degradation of KPNA1, representing a novel strategy for EV-A71 to evade host antiviral innate immunity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chunxia Wen, Yufeng Yu, Chengfeng Gao, Xian Qi, Carol J. Cardona, Zheng Xing
Summary: Zika virus can cause serious neurological disorders and induce cell death in macrophages, potentially impacting viral pathogenesis.
Article
Infectious Diseases
Yufeng Yu, Chengfeng Gao, Chunxia Wen, Peng Zou, Xian Qi, Carol J. Cardona, Zheng Xing
Summary: ZIKV NS4A inhibits viral RNA replication by activating the ISGF3 signaling pathway and inducing the production of ISGs, which may help ZIKV maintain its persistent infection in fetal neural tissues.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Virology
Chengfeng Gao, Yufeng Yu, Chunxia Wen, Zhifeng Li, Haida Ding, Xian Qi, Carol J. Cardona, Zheng Xing
Summary: Infection with SFTS virus can activate the NLRP3 inflammasome and induce IL-1 beta secretion in the brains of infected newborn mice. This study reveals a novel mechanism by which SFTSV dysregulates host responses through its nonstructural protein, providing insights into viral neuropathogenesis in SFTS patients.
JOURNAL OF VIROLOGY
(2022)