Article
Plant Sciences
Juri Saikia, Rajkumari Mazumdar, Debajit Thakur
Summary: This study investigated the cultivable endophytic actinobacteria associated with ten epiphytic orchid species in Assam, India. The isolates showed plant growth promotion (PGP) characteristics and antifungal activity, making them potential alternatives to chemical fertilizers and pesticides in agriculture.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Xue Zhang, Wenli Tian, Bijun Xie, Zhida Sun
Summary: This study evaluated the influence of fermentation on the levels of free amino acids and variations of volatile odorants in chickpea milk. The results showed that fermentation decreased the levels of free amino acids and led to remarkable changes in the types and contents of volatile odor substances.
Article
Food Science & Technology
Laura E. C. Noordraven, Carolien Buve, Tara Grauwet, Ann M. Van Loey
Summary: This study investigated the impact of flour preparation and thermal processing on the volatile profile of chickpea flour suspensions. Non-gelatinised flour suspensions contained higher levels of aldehydes, alcohols, and hydrocarbons, while pre-gelatinised open cell flour had higher levels of other compounds. Cell intactness had a limited effect on the volatile profile, with pre-gelatinised closed cell flour having the lowest number of discriminant compounds. Even after thermal processing, the volatile profiles of the three flour types remained distinct.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Plant Sciences
Yongzhou Li, Liangliang He, Yinhua Song, Peng Zhang, Doudou Chen, Liping Guan, Sanjun Liu
Summary: In this study, volatile compounds in the pulp and skin of five table grape cultivars with different aroma types were qualitatively analyzed. The analysis identified 215 aroma compounds, including esters, terpenes, and alcohols. The transcriptomic analysis and terpene profiling data revealed several candidate genes and transcription factors involved in aroma biosynthetic regulation. The results provide new insights into the regulation of different aromas in grapes.
Article
Plant Sciences
Renu Yadav, Rashmi Saini, Arindam Adhikary, Sanjeev Kumar
Summary: Drought and high temperature stress have an impact on chickpea growth and productivity. This study investigates the molecular and metabolic adaptations in two chickpea varieties with different heat tolerance under 35°C heat stress. The findings suggest that different genes and metabolites are involved in heat response and heat tolerance in these two varieties.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Horticulture
Baiqi Cui, Xue Zhao, Guoshuang Chen, Shijie Qin, Yitong Liu, Zhilin Han, Qian Yan, Luwen Han, Sijia Liu, Shuying Liu, Hongzhang Liu
Summary: In this study, two terpene synthase genes of Hosta flowers were cloned and characterized, and their function in the biosynthesis of terpenes was studied through analysis of volatile terpenes and gene expression levels. The findings provide insights into the terpene metabolic pathways in Hosta species.
Article
Agriculture, Multidisciplinary
Suzana Tiemi Ivamoto-Suzuki, Jose Miguel Celedon, Macaire M. S. Yuen, Ci Kitzberger, Douglas Silva Domingues, Jorg Bohlmann, Luiz Filipe Protasio Pereira
Summary: The biochemical profile of coffee beans determines the quality characteristics and health benefits of the coffee beverage. Ent-kaurene, a precursor of gibberellin synthesis in plants, also serves as an intermediate in specialized diterpenoid metabolism in coffee, leading to the production of more than 1,000 different metabolites. Diterpenes in coffee, including cafestol and kahweol, have nutraceutical effects such as antioxidant, anticarcinogenic, and anti-inflammatory properties. This study identified and characterized genes involved in the production of ent-kaurene in Coffea arabica, providing insights into the biosynthesis of cafestol and kahweol.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Yibo An, Xinyu Wang, Shida Ji, Jing Han, Yucheng Wang, Ling Ma, Zhihua Liu
Summary: The new Trichoderma asperellum strain TasT1 showed strong antifungal activity against Fusarium oxysporum Fox68 and induced the accumulation of defense-related enzymes in Arabidopsis thaliana seedlings. The fermentation liquor of TasT1 contained antibacterial substances and promoted the growth of A. thaliana seedlings. TasT1 has the potential to control F. oxysporum and boost disease resistance in plants.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Chemistry, Applied
Bing Lyu, Xiaoyan Zhang, Jingguang Li, Lei Zhang, Yuxin Zhong, Yongning Wu
Summary: The present study evaluated two GC-MS/MS instruments with different ion sources for detecting trace levels of PCDD/Fs in various foods. The GC-MS/MS methods showed good sensitivity, repeatability, and reproducibility, and the results were comparable to those obtained with GC-HRMS.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Applied
Mu Zhao, Ting Li, Fan Yang, Xinyue Cui, Tingting Zou, Huanlu Song, Ye Liu
Summary: This study characterized the composition of volatile aroma compounds and key aroma-active compounds in dried Hanyuan Zanthoxylum bungeanum. The results identified several key aroma-active compounds such as myrcene and limonene.
Article
Chemistry, Analytical
Chao Han, Chaoqun Huang, Qinting Jiang, Li Chen, Yi Li, Chengjie Lou, Hailing Lu, Yan Shen
Summary: A novel and accurate method for the determination of folpet and phthalimide residues in tea has been developed, using solid-phase extraction combined with gas chromatography-tandem mass spectrometry. The method proposes converting all folpet to phthalimide prior to sample extraction and purification, and utilizes an isotopic internal standard for quantification. The study confirms the accurate determination of phthalimide content and its conversion to folpet content.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Xuejie Li, Xiangquan Zeng, Huanlu Song, Yu Xi, Yan Li, Bowen Hui, He Li, Jian Li
Summary: GC x GC-O-TOF-MS and GC-IMS have been increasingly used in complex food flavor analysis, but few studies have applied these techniques to identify the aroma components of tomato pastes. This study comprehensively characterized the aroma profiles of cold and hot break tomato pastes using SAFE-GC-O-MS, SAFE-GC x GC-O-TOF-MS, and HS-GC-IMS for the first time. A total of 274 volatile compounds were identified, which is far more than previously reported.
Article
Biochemistry & Molecular Biology
Chen Chen, Hong Chen, Chao Han, Zemao Liu, Fangyuan Yu, Qikui Wu
Summary: This study investigated the effect of 24-epibrassinolide (EBL) on the concentration and composition of fatty acids (FA) in Styrax tonkinensis seed oil. Transcriptome sequencing and GC-MS were used to identify candidate genes involved in FA biosynthesis. The results showed that EBL5 treatment increased FA concentration and enhanced enzyme activity by promoting the expression of FA biosynthesis-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Yanqin Yang, Jialing Xie, Qiwei Wang, Yuliang Deng, Li Zhu, Jiayi Zhu, Haibo Yuan, Yongwen Jiang
Summary: This study comprehensively characterized the volatile and non-volatile compounds during black tea processing using volatolomics and UHPLC-Q-Exactive/MS analysis. The results showed that these metabolites changed continuously throughout the processing process, with significant changes during the withering stage. A total of 178 volatile metabolites and 103 non-volatile metabolites were identified, and 11 volatile components were identified as key aroma active components responsible for sweet aroma in finished black tea.
Article
Environmental Sciences
Zi-Feng Zhang, Jia-Cheng Chen, Ya-Xian Zhao, Lu Wang, Yu-Qian Teng, Ming-Hong Cai, Yan-Hui Zhao, Anatoly Nikolaev, Yi-Fan Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are toxic organic pollutants that have been widely detected in various regions and environmental matrices. However, not all PAHs are usually detected, and there is no established method to systematically analyze hundreds of PAHs. In this study, a gas chromatography-tandem triple-quadrupole mass spectrometry (GC-MS/MS) method was developed and successfully applied to identify and quantify 123 PAHs in marine samples.
News Item
Plant Sciences
A. Sarwar, Z. Latif, C. Cabaleiro
Article
Microbiology
Arslan Sarwar, Zakia Latif, Songya Zhang, Jing Zhu, David L. Zechel, Andreas Bechthold
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Parasitology
Muhammad Sohail, Zakia Latif
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2018)
Article
Biotechnology & Applied Microbiology
Aatif Amin, Arslan Sarwar, Mushtaq A. Saleem, Zakia Latif, Stanley J. Opella
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Microbiology
Arslan Sarwar, Zakia Latif, Songya Zhang, Jianjun Hao, Andreas Bechthold
FRONTIERS IN MICROBIOLOGY
(2019)
News Item
Plant Sciences
A. Sarwar, Z. Latif, C. Cabaleiro, A. Amin, M. A. Saleem
Article
Multidisciplinary Sciences
Tomas Adomavicius, Margherita Guaita, Yu Zhou, Martin D. Jennings, Zakia Latif, Alan M. Roseman, Graham D. Pavitt
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Dalaq Aiysha, Zakia Latif
Article
Biotechnology & Applied Microbiology
A. Sarwar, I. Ahmad, A. Amin, M. A. Saleem
Summary: This study analyzed the prevalence of antibiotic-resistant coliform bacteria isolated from paper currency in circulation in Pakistan, finding the highest bacterial population on currency from street vendors and butcher shops. Escherichia coli was the most prevalent coliform bacteria, with PCR amplification showing the presence of various antimicrobial resistance genes among coliform isolates. The study warns of the potential threat of severe bacterial infections due to improper hand hygiene and community sanitation when dealing with currency notes.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Iqra Zahid, Arslan Sarwar, Abid Hussain, Muhammad Sohail, Aatif Amin
Summary: This study aimed to evaluate the prevalence of XDR Salmonella among the local population of Lahore and genotype isolates for antibiotic-resistant genes. The study found a large number of clinical XDR S. Typhi-resistant against third generation cephalosporins have been reported, highlighting the possible emergence of clinical XDR S. Typhi cases in Lahore, Pakistan.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Zoology
Aatif Amin, Zakia Latif, Arslan Sarwar, Basit Zeshan, Mushtaq A. Saleem
Summary: Mercury resistant (Hg-R) bacteria were isolated from heavy metal polluted wastewater and soil near tanneries in Kasur, Pakistan. Phylogenetic analysis confirmed Bacillus sp. AZ-1, B. cereus AZ-2, B. cereus AZ-3, and Enterobacter cloacae ZA-15 as the resistant strains, with B. cereus strains showing the highest detoxification ability.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)