Article
Microbiology
Jodi M. Corley, Peter Intile, Timothy L. Yahr
Summary: The Gac/Rsm system is a global regulator of Pseudomonas aeruginosa gene expression, mediated by RsmA and RsmF RNA-binding proteins. The activity of RsmA/RsmF is controlled by small non-coding RNAs, and RsmA stimulates rsmY/rsmZ transcription by inhibiting RetS synthesis.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Menghao Yu, Youfu Zhao
Summary: Deletion of the CsrA gene in Lysobacter enzymogenes strain C3 resulted in slow growth and reduced resistance to multiple antibiotics. The deletion also affected its ability to inhibit hypha growth and its extracellular enzyme activities. Additionally, two putative small noncoding regulatory RNAs (sRNAs), csrB and csrC, were identified in the genome of LeC3. Double deletion of csrB and csrC increased resistance to antibiotics but did not affect biocontrol activity.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kotaro Chihara, Lars Barquist, Kenichi Takasugi, Naohiro Noda, Satoshi Tsuneda
Summary: This study detected over 500 binding sites of RsmA/N in Pseudomonas aeruginosa and verified a consensus motif for their binding. Genetic analysis combined with CLIP-seq results suggested previously unrecognized targets of RsmA/N involved in LPS modification, and proposed a possible mechanism for RsmA/N-mediated regulation of RsmY/RsmZ.
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Biochemistry & Molecular Biology
Chuan-min Zhou, Qun Wu, Biao Wang, Ping Lin, Min Wu, Xue-jie Yu
Summary: Calcium positively regulates the transcription of CRISPR-Cas system via LadS, while RsmA functions as a post-transcriptional regulator; the modulation of CRISPR-Cas system by calcium depends on LadS and RsmA, highlighting their previously unrecognized role in sensing calcium.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Lei Gao, Xinhua Qiao, Lixia Zhang, Yan Wang, Yi Wan, Chang Chen
Summary: The study found that nitric oxide can significantly reduce the expression of algU in Pseudomonas aeruginosa, thereby inhibiting the synthesis of alginate. This inhibitory effect was observed in nine clinical PA isolates, especially in mucoid strains.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Microbiology
Silvia Santoro, Costanza Paganin, Sara Gilardi, Tarcisio Brignoli, Giovanni Bertoni, Silvia Ferrara
Summary: The pathogenic lifestyle of Pseudomonas aeruginosa is based on the fine regulation of virulence-associated factors, which involves regulatory small RNAs and the RNA-binding protein Hfq. In this study, a novel sRNA called GssA was characterized and its multifaceted interplay with Hfq was investigated. GssA was found to be responsive to environmental and physiological signals and acted as a repressor of key bacterial functions in the human host.
Article
Microbiology
Qiuling Dong, Qing Yan, Bo Zhang, Li-qun Zhang, Xiaogang Wu
Summary: Pseudomonas fluorescens 2P24, a plant root-associated bacterium, produces the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) to suppress soilborne plant diseases. In this study, a new genetic element grxD was identified, which plays a crucial role in the production of 2,4-DAPG. Mutation of grxD significantly reduced 2,4-DAPG production and affected the function of other regulatory factors.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Anmin Ren, Minlu Jia, Jihong Liu, Tian Zhou, Liwen Wu, Tao Dong, Zhao Cai, Jiuxin Qu, Yang Liu, Liang Yang, Yingdan Zhang
Summary: This study characterized a P. aeruginosa clinical strain LYSZa7 and identified a T6SS lipase effector TseL(PA) that promotes bacterial pathogenesis by facilitating bacterial internalization into host cells. The findings suggest that clinical bacterial strains employ a diversified group of T6SS effectors for interbacterial competition and might contribute to the emergence of new epidemic clonal lineages.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jingyi Zhou, Shouyi Li, Haozhou Li, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Summary: A newly discovered TA system in Pseudomonas aeruginosa contributes to persister formation by reducing intracellular NAD(+) level, and this system is repressed by PA14_51020.
Article
Critical Care Medicine
Jill Zupetic, Hernan F. Penaloza, William Bain, Mei Hulver, Roberta Mettus, Peter Jorth, Yohei Doi, Jennifer Bomberger, Joseph Pilewski, Mehdi Nouraie, Janet S. Lee
Summary: The study found that elastase activity of Pseudomonas aeruginosa (PA) was associated with increased 30-day mortality in ICU patients. Mice infected with a high elastase producer showed increased lung bacterial burden and inflammatory profile compared to those infected with a low elastase producer.
Article
Agricultural Engineering
Binhan Zhao, Xianhe Cao, Zhanjun Cai, Long Zhang, Dajun Li, Haoyue Zhang, Shaojie Li, Xianyun Sun
Summary: Fifty microbial strains with antifungal activity were isolated and identified from composting cow manure. Two bacterial strains, B282 and F288, were found to be antagonistic against both phytopathogenic fungi and bacteria. Inoculation of these strains significantly changed the microbial community structure of compost and increased the antimicrobial activity and disease suppressive effects of compost.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Xu Yanhua, Lou Haiwei, Zhao Renyong
Summary: This study successfully obtained recombinant catalase through gene engineering and demonstrated its effectiveness in degrading AFB(1), providing a new paradigm for degrading aflatoxins in food and feed using recombinant catalases.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Infectious Diseases
Xavier Vuillemin, Maelle Da Silvab, Maxime Bour, Celine Landond, Patrick Plesiat, Katy Jeannot
Summary: This study evaluated the impact of certain acquired extended-spectrum oxacillinases (ES-OXA) on the susceptibility of Pseudomonas aeruginosa to cefiderocol. It was found that certain variant ES-OXA significantly decreased the susceptibility of Pseudomonas aeruginosa to cefiderocol.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)