Article
Microbiology
Laura Elpers, Lena Lueken, Fabio Lange, Michael Hensel
Summary: The number of outbreaks caused by gastrointestinal pathogens associated with produce is increasing. The mechanisms involved in the colonization, persistence, and transmission of these pathogens on fresh produce are poorly understood. This study focused on the adhesive factors of S. enterica serovar Typhimurium on butterhead lettuce (Lactuca sativa) and identified several factors that contribute to its adhesion to lettuce leaves.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Xin Zheng, Hongjuan Bai, Ye Tao, Mounia Achak, Yannick Rossez, Edvina Lamy
Summary: This study analyzed the transport and deposition behavior of Salmonella enterica serovar Typhimurium with different flagellar phenotypes in porous media. The results showed that flagellar motility promotes bacterial transport and deposition, and non-motile flagella enhance bacterial adhesion. Flagella phases and methylation also affect bacterial movement and attachment capabilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ixchell Y. Sedillo-Torres, alvaro O. Hernandez-Rangel, Yolanda Gomez-y-Gomez, Daniel Cortes-Avalos, Blanca Estela Garcia-Perez, Juan C. Villalobos-Rocha, Cesar H. Hernandez-Rodriguez, Luis Gerardo Zepeda-Vallejo, Paulina Estrada-de los Santos, Maria Elena Vargas-Diaz, Jose Antonio Ibarra
Summary: An organic extract of Hibiscus sabdariffa L. containing hibiscus acid was found to inhibit motility and reduce the secretion of virulence factors in Salmonella enterica serovars Typhi and Typhimurium. The compound was not toxic to eukaryotic cells and could reduce the invasion of Salmonella Typhimurium in epithelial cells. Initial studies suggested that the mode of action of hibiscus acid might involve the disruption of membrane proton balance.
Article
Immunology
Fei Lou, Kunli Wang, Yunfeng Hou, Xiaolei Shang, Fayin Tang
Summary: Resveratrol was found to inhibit the flagella-mediated virulence and adhesion of S. typhimurium to cells. Although it did not significantly improve the survival rate of infected mice, resveratrol reduced the bacterial load in the liver and spleen and showed anti-inflammatory activity.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Yunjie Chang, Hui Xu, Md A. Motaleb, Jun Liu
Summary: Researchers have discovered a multiprotein collar complex in Lyme disease spirochetes, which exhibits remarkable structural plasticity to accommodate the highly curved membrane and generate the high torque necessary for spirochete motility.
Article
Infectious Diseases
Martin Zermeno-Ruiz, Itzia A. Rangel-Castaneda, Daniel Osmar Suarez-Rico, Leonardo Hernandez-Hernandez, Rafael Cortes-Zarate, Jose M. Hernandez-Hernandez, Gabriela Camargo-Hernandez, Araceli Castillo-Romero
Summary: The study found that curcumin had no antibacterial activity against Salmonella enterica serovar Typhimurium, even at higher concentrations. Moreover, curcumin caused overgrowth of the bacteria and increased their pathogenicity. It also shortened the lifespan and survival time of Caenorhabditis elegans when they were fed curcumin-treated bacteria. These findings suggest that the indiscriminate use of curcumin should be reconsidered, especially in outbreaks of pathogenic Gram-negative bacteria.
Article
Microbiology
Julian Schwanbeck, Ines Oehmig, Uwe Gross, Wolfgang Bohne
Summary: This study systematically investigated the effect of various amino acids and carbohydrates on the swimming velocity of Clostridioides difficile. It was found that proline and cysteine are the most important amino acids for powering swimming motility, while glycine is not critical. Additionally, a maximal swimming motility can be achieved with only four compounds, including specific amino acids and interchangeable carbohydrate sources.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Microbiology
Yan Li, Pei Zhang, Pengcheng Du, Yujiao Mu, Shenghui Cui, Seamus Fanning, Zhiqiang Wang, Ruichao Li, Li Bai
Summary: Hybrid plasmids formed by fusion events in Salmonella enterica serovar 4,[5],12:i:- possess high plasticity and can recombine with other plasmids to form fusion plasmids of different sizes. The structural polymorphisms of plasmids are mainly mediated by insertion sequences and can rearrange the internal structures of the plasmids. This study provides insights into the transmission risk and evolution of multidrug resistance plasmids.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Manal Mohammed, Beata Orzechowska
Summary: The study aimed to characterize phage resistance mechanisms and genomic differences in S. Typhimurium DT104 and DT104b that may explain divergent phage reaction patterns, but found no unique genetic determinants. Experimental assessment of phage-specific receptors on the bacterial cell surface and analysis of bacterial transcriptome using RNA sequencing were suggested to further understand bacterial susceptibility to phages. The use of Anderson phage typing scheme of Salmonella Typhimurium can help improve the understanding of host-phage interactions and lead to the development of phage-based technologies for infection control.
Article
Microbiology
Claudia A. Cox, Marek Bogacz, Faiha M. El Abbar, Darren D. Browning, Brian Y. Hsueh, Chris M. Waters, Vincent T. Lee, Stuart A. Thompson
Summary: Campylobacter jejuni is a leading cause of bacterial gastroenteritis and is associated with various extragastrointestinal conditions. The study found that CbrR is a negative regulator that controls motility and the expression of FlaA, a critical virulence factor in C. jejuni pathogenesis.
Article
Multidisciplinary Sciences
Danielle J. Ingle, Rebecca L. Ambrose, Sarah L. Baines, Sebastian Duchene, Anders Goncalves da Silva, Darren Y. J. Lee, Miriam Jones, Mary Valcanis, George Taiaroa, Susan A. Ballard, Martyn D. Kirk, Benjamin P. Howden, Jaclyn S. Pearson, Deborah A. Williamson
Summary: Three distinct lineages of Salmonella 4,[5],12:i:- have emerged in Australia, likely reflecting differential antimicrobial selection pressures, with two lineages associated with travel to South-East Asia and the third lineage endemic to Australia. These antimicrobial-resistant lineages efficiently infect and survive in host cells, suggesting a suppression of host immune response facilitating their persistence.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Yu Han, Yu Li, Zhiwei Zeng, Wenjing Li, Saixiang Feng, Weisheng Cao
Summary: This study explores the resistance mechanism and activity of the antimicrobial peptide MccY against Salmonella strains. The deletion of Ton system genes resulted in resistance to MccY and mutants showed increased susceptibility to MccY under a low-iron condition. MccY may decrease the virulence of S. Typhimurium by altering multiple physiological properties.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Chong Ling, Shujie Liang, Yan Li, Qingyun Cao, Hui Ye, Changming Zhang, Zemin Dong, Dingyuan Feng, Weiwei Wang, Jianjun Zuo
Summary: This study reveals that STM0306 is an important adhesin/invasin in Salmonella enterica serovar typhimurium (S. Typhimurium), contributing to the pathogenicity and cellular inflammation of the host.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Swarupa Mallick, Neha Mishra, Bedanta Kumar Barik, Vidya Devi Negi
Summary: This study aims to explore the development of C. elegans dauer larvae upon Salmonella Typhimurium infection and investigate the role of bacterial genes in regulating the worm's behavioral plasticity. The results revealed that the deletion of the fepB gene in the Salmonella strain reduced pathogenicity and bacterial burden in the worm's gut without damaging the pharynx. The fepB mutant strain enhanced the worm's immune responses and activated dauer signaling via the TGF-beta pathway, leading to increased dauer formation.
JOURNAL OF INFECTION
(2022)
Article
Immunology
Neha Mishra, Swarupa Mallick, Vidya Devi Negi
Summary: This study reveals that Salmonella infection causes a 50% reduction in the lifespan of Caenorhabditis elegans. The infection leads to egg retention in the worm, resulting in developmental and morphological defects as well as disruption of developmental timing regulation. The infected eggs show various abnormalities, including over-folding of the developing embryo, increased size, and reduced osmotic stress resistance. The infection also delays and reduces hatching of the eggs, while accelerating the development of the L3, L4, and adult stages. These findings shed light on the mechanisms of Salmonella infection and its impact on the lifespan of the worm.
MICROBES AND INFECTION
(2022)
Article
Physics, Applied
N. Punith, Ashish K. Singh, J. Ananthanarasimhan, Bhavadharini Boopathy, Ritika Chatterjee, M. Hemanth, Dipshikha Chakravortty, Lakshminarayana Rao
Summary: This study demonstrates the successful generation of neutral pH high-strength plasma-activated water through pin to water discharge, which exhibits excellent bactericidal activity against hypervirulent multidrug resistance pathogens.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Chemistry, Physical
Abdur Rasheed, Omkar Hegde, Ritika Chatterjee, Srinivas Rao Sampathirao, Dipshikha Chakravortty, Saptarshi Basu
Summary: In naturally evaporating droplets, bacteria self-assemble into unique patterns based on their spatial location, resulting in varying physical forces and affecting their viability and infectivity. Self-assembly favors bacteria at the edge of the droplet, which has significant implications for understanding bacterial infection through droplets.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Immunology
Dipasree Hajra, Abhilash Vijay Nair, Atish Roy Chowdhury, Soumyadeep Mukherjee, Ritika Chatterjee, Dipshikha Chakravortty
Summary: This study reveals a novel role of U32 peptidase YdcP in Salmonella Typhimurium by protecting the bacteria from oxidative stress. The knockout of YdcP leads to attenuated intracellular proliferation of Salmonella and increased susceptibility to in vitro oxidative stress.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Jayantika Bhowmick, Manish Nag, Pritha Ghosh, Raju S. Rajmani, Ritika Chatterjee, Kapudeep Karmakar, Kasturi Chandra, Jayanta Chatterjee, Dipshikha Chakravortty, Raghavan Varadarajan
Summary: This study tests the effect of a 24-mer peptide derived from CcdB (CP1-WT) on bacterial growth in Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus, and a carbapenem- and tigecycline-resistant strain of Acinetobacter baumannii. The CP1-WT peptide shows significant improvement over ciprofloxacin in treating established infections of S. Typhimurium, S. aureus, and A. baumannii. The study validates the CcdB binding site on bacterial DNA Gyrase as a viable alternative target to the fluoroquinolone binding site.
Article
Immunology
Ritika Chatterjee, Debalina Chaudhuri, Subba Rao Gangi Setty, Dipshikha Chakravortty
Summary: Salmonella is a stealthy intracellular pathogen that evades host immune responses and establishes a replicative niche in hostile environments like macrophages. It escapes autophagy by inhibiting the fusion of Salmonella-containing vacuoles with lysosomes/autophagosomes and downregulates lysosomal biogenesis, facilitating its survival in macrophages and systemic dissemination.
MICROBES AND INFECTION
(2023)
Article
Cell Biology
Ritika Chatterjee, Abhilash Vijay Nair, Anmol Singh, Nishi Mehta, Subba Rao Gangi Setty, Dipshikha Chakravortty
Summary: Intracellular membrane fusion is mediated by SNARE complexes. Salmonella modulates host SNARE machinery to escape lysosomal fusion and maintain its division in the Salmonella-containing vacuole (SCV). Syntaxin 3 (STX3) is critical for bacterial proliferation and fusion of SCV with intracellular vesicles. The interaction of STX3 with SCV is impaired in SPI-2 encoded T3SS mutant. These findings highlight the importance of SPI-2 encoded effectors in the interaction with host SNAREs for Salmonella pathogenesis.
Article
Multidisciplinary Sciences
Vishnu Hariharan, Atish Roy Chowdhury, S. Srinivas Rao, Dipshikha Chakravortty, Saptarshi Basu
Summary: This study reveals the impact of bacteria-laden droplets on solid surfaces and subsequent desiccation on the virulence of Salmonella typhimurium. The research explores the deformation, cell-cell interactions, adhesion energy, and roughness in bacteria induced by impact velocity and low moisture. The findings show that mechanical stress induced by impact velocity decreases the in vitro viability of Salmonella while promoting bacterial proliferation in macrophages. The role of phoP in sensing mechanical stress and maintaining the virulence of Salmonella is also highlighted.
Article
Mathematical & Computational Biology
Ranjitha Guttapadu, Kalyani Korla, Safnaz Uk, Vamseedhar Annam, Purnima Ashok, Nagasuma Chandra
Summary: Type 2 Diabetes (T2D) is commonly treated with metformin, but many patients still develop complications. By analyzing transcriptomic data of T2D subjects, we constructed a protein-protein interaction network that captures the global perturbations in diabetes. We identified possible drug targets related to oxidative stress and hypercholesterolemia and proposed Probucol as a potential co-drug with metformin. In a rat model of diabetes, the combination of metformin and Probucol at 5:0.5 mg/kg effectively restored near-normal levels of glucose, lipids, and cholesterol.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Chemistry, Medicinal
Ushashi Banerjee, Adithya Chedere, Raksha Padaki, Abhilash Mohan, Narmada Sambaturu, Amit Singh, Nagasuma Chandra
Summary: In this study, a new tool called PathTracer was developed to identify biologically relevant information flows by mining genome-wide protein-protein interaction networks integrated with gene expression data. The utility of this tool was demonstrated by identifying adaptation mechanisms of hypoxia-induced dormancy in Mycobacterium tuberculosis (Mtb).
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Microbiology
Kasturi Chandra, Abhilash Vijay Nair, Ritika Chatterjee, Prerana Muralidhara, Anmol Singh, Sathisha Kamanna, Utpal S. Tatu, Dipshikha Chakravortty
Summary: Salmonella is a widespread Gram negative bacteria that causes a significant proportion of global diarrheal morbidity and mortality. It can cause typhoid fever and gastroenteritis by entering the host gut through contaminated food and water. Salmonella's biofilm lifestyle allows it to resist antibiotics and persist in the host, and inhibiting biofilm initiation is challenging.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Ritika Chatterjee, Atish Roy Chowdhury, Debapriya Mukherjee, Dipshikha Chakravortty
Summary: Salmonella Typhi is a serious threat to global health, causing typhoid fever in humans and resulting in high morbidity and mortality in developing countries. It can be transmitted to healthy individuals through contaminated food and water. This Review focuses on a comparative analysis of the virulence and pathogenesis of typhoidal and nontyphoidal serovars of Salmonella enterica.
Article
Biochemistry & Molecular Biology
Santhosh Sankar, Preeti Preeti, Kavya Ravikumar, Amrendra Kumar, Yedu Prasad, Sukriti Pal, Desirazu N. Rao, Handanahal S. Savithri, Nagasuma Chandra
Summary: This study comprehensively analyzed the structures of SAM binding sites and identified four different types of binding sites. The common minimum principle and structural motifs involved in SAM recognition were deciphered for each site type. The study also found high similarity between SAM binding sites and ATP binding sites, providing evidence for convergent evolution. These findings offer molecular-level insights into how diverse proteins recognize and bind SAM.
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Physical
Siddhant Jain, Anmol Singh, Nivedita Tiwari, Aparna Naik, Ritika Chatterjee, Dipshikha Chakravortty, Saptarshi Basu
Summary: This study experimentally investigates the effects of stress generated by flowing fluid on bacterial morphology and virulence. The results show that increased stress conditions lead to significant changes in bacterial structure and decreased viability. Importantly, stressed bacteria proliferate faster inside macrophages. These findings shed light on the complex relationship between flow stresses and bacterial pathogenicity.