Review
Biotechnology & Applied Microbiology
Shomita S. Mathew-Steiner, Sashwati Roy, Chandan K. Sen
Summary: Normal wound healing progresses through inflammatory, proliferative and remodeling phases, with collagen playing a key role in regulating these phases. Impairments in wound healing can lead to chronic non-healing wounds, where collagen has been utilized as an adjunct therapy to promote healing.
BIOENGINEERING-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Collins N. Elangwe, Svetlana N. Morozkina, Roman O. Olekhnovich, Victoria O. Polyakova, Alexander Krasichkov, Piotr K. Yablonskiy, Mayya V. Uspenskaya
Summary: Wound healing is a complex process aimed at creating new tissues and restoring anatomical functions. Wound dressings, made from natural or synthetic biomaterials, play a crucial role in protecting the wound and accelerating healing. Polysaccharide polymers, particularly pullulan, have gained attention for their non-toxic, antibacterial, biocompatible, hemostatic, and nonimmunogenic properties. Hydrogels based on pullulan have shown promise as wound dressings due to their high water retention capacity, which creates a moist environment and promotes wound healing. The incorporation of pullulan with other biocompatible polymers, such as chitosan and gelatin, has been explored to enhance its properties for wound dressing applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Punnida Nonsuwan, Preeyaporn Plaimee Phiboonchaiyanan, Namon Hirun, Pakorn Kraisit
Summary: A Pul-derivative hydrogel was developed by introducing methacrylate groups and beta-cyclodextrin to improve mechanical properties and increase curcumin solubility. The hydrogel exhibited enhanced curcumin solubility, biocompatibility, and accelerated wound healing.
CARBOHYDRATE POLYMERS
(2023)
Review
Chemistry, Multidisciplinary
Yongping Liang, Jiahui He, Baolin Guo
Summary: Hydrogels have shown promising advantages as wound dressings due to their excellent biochemical and mechanical properties. This review summarizes the advanced functions of hydrogel dressings, such as antimicrobial, adhesion, hemostasis, anti-inflammatory, substance delivery, self-healing, stimulus response, and conductivity, as well as the emerging wound monitoring feature. The potential applications for different types of wounds, including chronic wounds and focus on infected, burn, and diabetic wounds, are discussed, along with proposed future directions for hydrogel wound dressings in wound healing.
Article
Biochemistry & Molecular Biology
Lanju Xu, Yufei Liu, Lizong Tang, Hui Xiao, Zhuo Yang, Shufang Wang
Summary: The study demonstrates that the rhCol III-EVs hydrogel enhances skin wound healing by promoting cell type transformation, cell migration, and angiogenesis, showing promising results in a diabetic rat skin injury model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Andreu Miquel Amengual-Tugores, Carmen Raez-Meseguer, Maria Antonia Forteza-Genestra, Marta Monjo, Joana M. Ramis
Summary: Hydrogels and extracellular vesicle-based therapies have shown promise in wound closure, particularly in managing chronic and acute wounds. The unique properties of hydrogels enable sustained and controlled release of EVs and optimal pH maintenance. Despite the potential benefits, challenges such as producing functional EV-loaded hydrogels and identifying suitable long-term storage conditions for EVs need to be addressed to facilitate the translation of this therapy into clinical practice. This review aims to discuss reported EV-based hydrogel combinations, their outcomes, and future prospects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Engineering, Biomedical
Renata Sklenarova, Naoufal Akla, Meagan Jade Latorre, Jitka Ulrichova, Jana Frankova
Summary: During wound healing, various cells are activated and the deposition of collagen, one of the most abundant structural proteins, plays a crucial role. This review discusses the impact of different collagen implants on corneal and skin wound healing.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Dermatology
Mohammadreza (Sam) Pakyari, Reza B. Jalili, Ruhangiz Taghi Kilani, Nafise Amiri, Erin Brown, Aziz Ghahary
Summary: The study confirmed that the liquid dermal scaffold MeshFill has a high efficacy in the healing process of chronic wounds, effectively filling tunnels and cavities, accelerating wound closure, and being similar to normal skin anatomy, supporting cell growth.
EXPERIMENTAL DERMATOLOGY
(2022)
Review
Cell & Tissue Engineering
Linda Vriend, Viktor Sinkunas, Cristina P. Camargo, Berend van der Lei, Martin C. Harmsen, Joris A. van Dongen
Summary: ECM hydrogels show promising therapeutic efficacy in enhancing dermal wound healing by reducing wound size and promoting angiogenesis, with no serious complications reported. However, further clinical studies are needed to draw firm conclusions on their efficacy and mechanisms of action.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Review
Polymer Science
Elena Iulia Oprita, Andreea Iosageanu, Oana Craciunescu, Zhiyuan Jia, Holger Schoenherr, Nowsheen Goonoo
Summary: Diabetic chronic wounds are characterized by prolonged and disorganized stages compared to normal wound healing. Natural polymer hydrogels, with their high water content and biocompatibility, show great potential as wound dressings. Natural bioactive hydrogels loaded with compounds such as antibacterials and antioxidants can modulate inflammation and adhere to wounded tissue, providing promising applications for diabetic wound healing. Natural bioactive compounds like polyphenols, polysaccharides, and proteins have multiple advantages in promoting chronic wound healing in diabetes, including their antioxidant, anti-inflammatory, antimicrobial, anti-allergic, and wound healing properties.
Review
Biotechnology & Applied Microbiology
Victor Alfonso Solarte David, Viviana Raquel Guiza-Argueello, Martha L. Arango-Rodriguez, Claudia L. Sossa, Silvia M. Becerra-Bayona
Summary: The absence or damage of tissue is a major cause of diseases, and novel therapeutic alternatives aim to recover lost functions through tissue regeneration. Chronic cutaneous lesions are common wounds that require regenerative medicine and tissue engineering interventions to develop bioactive medical products for proper healing and prevention of complications. The coordination of cells, extracellular matrix (ECM), and biomolecules is crucial in tissue repair and regeneration, with ECM acting as a biological platform for their interactions. Tissue engineering utilizes synthetic polymers and decellularized tissues to mimic native ECM and provide necessary functionalities for cell differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yanling Song, Yuchan You, Xinyi Xu, Jingyi Lu, Xiajie Huang, Jucong Zhang, Luwen Zhu, Jiahao Hu, Xiaochuan Wu, Xiaoling Xu, Weiqiang Tan, Yongzhong Du
Summary: The ECM@exo treatment, which incorporates ADSC-exos into an ECM hydrogel, shows great potential in promoting wound healing by reducing inflammation, enhancing cell proliferation and migration, as well as promoting angiogenesis and collagen deposition.
Review
Nanoscience & Nanotechnology
Jia-Ying Ji, Dan-Yang Ren, Ying-Zheng Weng
Summary: This review article discusses the recent developments and innovations of antibacterial hydrogels for diabetic chronic wound healing.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Engineering, Biomedical
Huimin Xiao, Xin Chen, Xuanzhe Liu, Gen Wen, Yaling Yu
Summary: The skin is an important organ that protects the body and interacts with the external environment. Decellularized biomaterials derived from the extracellular matrix have shown potential in promoting wound healing and skin regeneration. This article reviews the wound healing process, the role of extracellular matrix constituents in wound healing, and the various types of decellularized materials used in clinical practice. The challenges and future research directions in utilizing decellularized biomaterials for wound treatment are also discussed.
MATERIALS TODAY BIO
(2023)
Article
Cell & Tissue Engineering
Janos A. Barrera, Artem A. Trotsyuk, Zeshaan N. Maan, Clark A. Bonham, Madelyn R. Larson, Paul A. Mittermiller, Dominic Henn, Kellen Chen, Chyna J. Mays, Smiti Mittal, Alana M. Mermin-Bunnell, Dharshan Sivaraj, Serena Jing, Melanie Rodrigues, Sun Hyung Kwon, Chikage Noishiki, Jagannath Padmanabhan, Yuanwen Jiang, Simiao Niu, Mohammed Inayathullah, Jayakumar Rajadas, Michael Januszyk, Geoffrey C. Gurtner
Summary: In this study, adipose-derived stem cell (ASC)-seeded hydrogels were found to improve wound healing following thermal injury in a murine model. The ASC hydrogel treatment accelerated reepithelialization, increased vascularity, and reduced scar formation compared to control groups. Furthermore, enriching ASCs for specific markers resulted in improved tissue architecture and collagen remodeling postburn injury.
TISSUE ENGINEERING PART A
(2021)
Article
Surgery
Ashish R. Chowdary, Tristan Maerz, Dominic Henn, Kurt D. Hankenson, Chase A. Pagani, Simone Marini, Katherine Gallagher, Carlos A. Aguilar, Robert J. Tower, Benjamin Levi
Summary: The objective of this study was to identify macrophage subpopulations and gene signatures associated with different types of musculoskeletal injury. Through single-cell RNA sequencing data analysis, distinct subpopulations of macrophages were identified in regenerative and fibrotic healing, and it was found that the transcriptional profiles of these subpopulations had similarities in cell composition. Macrophage polarization state was not correlated with healing outcomes, but the analysis of receptor/ligand interaction between macrophages and mesenchymal progenitor cells showed potential therapeutic targets for predicting fibrotic responses and promoting regenerative conditions.
Article
Biotechnology & Applied Microbiology
Yuanwen Jiang, Artem A. Trotsyuk, Simiao Niu, Dominic Henn, Kellen Chen, Chien-Chung Shih, Madelyn R. Larson, Alana M. Mermin-Bunnell, Smiti Mittal, Jian-Cheng Lai, Aref Saberi, Ethan Beard, Serena Jing, Donglai Zhong, Sydney R. Steele, Kefan Sun, Tanish Jain, Eric Zhao, Christopher R. Neimeth, Willian G. Viana, Jing Tang, Dharshan Sivaraj, Jagannath Padmanabhan, Melanie Rodrigues, David P. Perrault, Arhana Chattopadhyay, Zeshaan N. Maan, Melissa C. Leeolou, Clark A. Bonham, Sun Hyung Kwon, Hudson C. Kussie, Katharina S. Fischer, Gurupranav Gurusankar, Kui Liang, Kailiang Zhang, Ronjon Nag, Michael P. Snyder, Michael Januszyk, Geoffrey C. Gurtner, Zhenan Bao
Summary: Smart bandages based on multimodal wearable devices have the potential to monitor and intervene in the healing process of chronic wounds. However, the integration of sensors and stimulators in current smart bandage technologies is limited. In this study, a flexible bioelectronic system with wireless power, closed-loop sensing and stimulation circuits, and skin-interfacing hydrogel electrodes with switchable adhesion was developed to address these challenges. The wound care system showed improved healing and tissue regeneration in preclinical wound models.
NATURE BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Alexander Stahl, Dake Hao, Janos Barrera, Dominic Henn, Sien Lin, Seyedsina Moeinzadeh, Sungwoo Kim, William Maloney, Geoffrey Gurtner, Aijun Wang, Yunzhi Peter Yang
Summary: Conventional synthetic vascular grafts have high failure rates due to mismatched mechanical properties with native vessels and poor regenerative potential. In this study, we developed a new bioactive synthetic vascular graft with tailored pore sizes to promote pro-regenerative macrophage populations and improve vascular regeneration. The grafts showed similar mechanical properties to native blood vessels and maintained patency and vascular regeneration in animal models.
BIOACTIVE MATERIALS
(2023)
Article
Surgery
Janos A. Barrera, Artem A. Trotsyuk, Dominic Henn, Dharshan Sivaraj, Kellen Chen, Smiti Mittal, Alana M. Mermin-Bunnell, Madelyn R. Larson, Jagannath Padmanabhan, Brian Kinney, James Nachbar, Justin Sacks, Sarvam P. Terkonda, Lynn Jeffers, Geoffrey C. Gurtner
Summary: Blockchain technology has gained significant attention globally and has been explored in various applications in the healthcare domain. However, its integration in plastic surgery is still in its early stages and requires further research and development.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Medicine, General & Internal
Dharshan Sivaraj, Chikage Noishiki, Nina Kosaric, Harriet Kiwanuka, Hudson C. Kussie, Dominic Henn, Katharina S. Fischer, Artem A. Trotsyuk, Autumn H. Greco, Britta A. Kuehlmann, Filiberto Quintero, Melissa C. Leeolou, Maia B. Granoski, Andrew C. Hostler, William W. Hahn, Michael Januszyk, Ferid Murad, Kellen Chen, Geoffrey C. Gurtner
Summary: According to the American Diabetes Association, there is a significant unmet need for new therapies to accelerate the closure of non-healing wounds. In this study, the effects of NO-releasing gel on diabetic wound healing in mice were investigated. The results showed that topical administration of NO-gel significantly accelerated the rate of wound healing and promoted a more regenerative ECM architecture.
FRONTIERS IN MEDICINE
(2023)
Editorial Material
Ethics
Lea Goetz, Markus Trengove, Artem Trotsyuk, Carole A. Federico
AMERICAN JOURNAL OF BIOETHICS
(2023)
Article
Surgery
Dominic Henn, Artem A. Trotsyuk, Janos A. Barrera, Dharshan Sivaraj, Kellen Chen, Smiti Mittal, Alana M. Mermin-Bunnell, Arhana Chattopadhyay, Madelyn R. Larson, Brian M. Kinney, James Nachbar, Sarvam P. TerKonda, Sashank Reddy, Lynn Jeffers, Justin M. Sacks, Geoffrey C. Gurtner
Summary: Although robotic surgery has been widely adopted in other surgical disciplines, it has been less utilized in plastic surgery. Recent advances in robotics and AI are gaining momentum and have significant potential to improve patient care in plastic surgery.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Multidisciplinary Sciences
Dominic Henn, Dehua Zhao, Dharshan Sivaraj, Artem Trotsyuk, Clark Andrew Bonham, Katharina S. Fischer, Tim Kehl, Tobias Fehlmann, Autumn H. Greco, Hudson C. Kussie, Sylvia E. Moortgat Illouz, Jagannath Padmanabhan, Janos A. Barrera, Ulrich Kneser, Hans-Peter Lenhof, Michael Januszyk, Benjamin Levi, Andreas Keller, Michael T. Longaker, Kellen Chen, Lei S. Qi, Geoffrey C. Gurtner
Summary: Chronic wounds pose a significant healthcare burden, but cell-based therapies have not been widely adopted. Researchers applied CRISPR/Cas9 to edit murine dendritic cells and enhance their therapeutic potential for healing chronic wounds.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Jagannath Padmanabhan, Kellen Chen, Dharshan Sivaraj, Dominic Henn, Britta A. Kuehlmann, Hudson C. Kussie, Eric T. Zhao, Anum Kahn, Clark A. Bonham, Teruyuki Dohi, Thomas C. Beck, Artem A. Trotsyuk, Zachary A. Stern-Buchbinder, Peter A. Than, Hadi S. Hosseini, Janos A. Barrera, Noah J. Magbual, Melissa C. Leeolou, Katharina S. Fischer, Seth S. Tigchelaar, John Q. Lin, David P. Perrault, Mimi R. Borrelli, Sun Hyung Kwon, Zeshaan N. Maan, James C. Y. Dunn, Rahim Nazerali, Michael Januszyk, Lukas Prantl, Geoffrey C. Gurtner
Summary: Small animals do not replicate the severity of the human foreign-body response (FBR) to implants. However, the FBR can be induced in mice to mimic human-like FBR through specific immune cell signaling and can be mitigated by inhibiting the signaling molecule Rac2.
NATURE BIOMEDICAL ENGINEERING
(2023)
Meeting Abstract
Cell Biology
Artem A. Trotsyuk, Serena Jing, Kellen Chen, Dominic Henn, Yuanwen Jiang, Simiao Niu, Dharshan Sivaraj, Ronjon Nag, Michael Snyder, Zhenan bao, Geoffrey C. Gurtner
WOUND REPAIR AND REGENERATION
(2023)
Meeting Abstract
Cell Biology
Artem A. Trotsyuk, Yuanwen Jiang, Simiao Niu, Kellen Chen, Dominic Henn, Dharshan Sivaraj, Zhenan Bao, Geoffrey C. Gurtner
WOUND REPAIR AND REGENERATION
(2023)
Article
Biology
Katharina S. Fischer, Ben Litmanovich, Dharshan Sivaraj, Hudson C. Kussie, William W. Hahn, Andrew C. Hostler, Kellen Chen, Geoffrey C. Gurtner
Summary: This article presents a method for studying wound healing using a rodent experimental model, specifically the splinted excisional wound model. This model is simple to use and provides a simulation of human wound healing process.