Article
Biotechnology & Applied Microbiology
Catherine Hennechart-Collette, Gaelle Gonzalez, Lisa Fourniol, Audrey Fraisse, Cecile Beck, Sara Moutailler, Laure Bournez, Nolwenn M. Dheilly, Sandrine A. Lacour, Sylvie Lecollinet, Sandra Martin-Latil, Sylvie Perelle
Summary: A molecular method based on the international standard was developed to detect TBEV in raw milk products. The recovery rates of TBEV varied under different inoculation levels and conditions. The sensitivity and reliability of the method were confirmed, making it suitable for routine diagnosis and assessment of potential health risks.
Article
Food Science & Technology
Chenang Lyu, Feifeng Lu, Zhentao Shi, Dapeng Wang
Summary: The study developed an ISC-RT-qPCR assay for detecting encapsulated RVA in oysters with higher sensitivity and accuracy than RT-qPCR. When applied to oysters collected from four different farms, ISC-RT-qPCR showed a higher detection rate of RVA compared to RT-qPCR, indicating its potential as a better method for disease monitoring and control.
Article
Agriculture, Dairy & Animal Science
Jose G. Olveira, Sandra Souto, Isabel Bandin, Carlos P. Dopazo
Summary: In this study, a reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) was developed for the detection and quantification of the fish virus NNV with high sensitivity and reliability, allowing for accurate diagnosis and quantification at extremely low viral loads.
Article
Plant Sciences
Shaonpius Mondal, Laura Jenkins Hladky, William M. Wintermantel
Summary: Viruses transmitted by the whitefly pose a growing threat to cucurbit production worldwide. Researchers have developed a multiplex RT-PCR method to identify and quantify the prevalence and distribution of these viruses. The study reveals differences in the prevalence of different viruses during different seasons and competitive accumulation of viruses within melon plants. This study also provides the first report of SqVYV in Arizona and offers an efficient method for virus detection and quantification.
Article
Plant Sciences
Shaonpius Mondal, Laura Jenkins Hladky, William M. Wintermantel
Summary: Viruses transmitted by whiteflies pose a growing threat to cucurbit production worldwide. A study developed a multiplex RT-PCR method and TaqMan probes for the detection and quantification of these viruses in melon samples from California and Arizona. The study found differences in the prevalence and distribution of the viruses during different seasons and within different parts of the plants.
Article
Agronomy
Melina Simeone, Claudio Gomez, Ana Bertalmio, Esperanza Ruiz, Claudia Hauteville, Laura Godoy Suarez, Blas Tito, Maria L. Garcia
Summary: Citrus psorosis virus (CPsV) is the main causal agent of citrus psorosis, and it is commonly diagnosed using biological indexing. This study showed that RT-qPCR is equivalent to biological indexing for diagnosing citrus psorosis, providing confidence in the quick diagnostic procedure for monitoring the health status of citrus trees.
Review
Environmental Sciences
Vu Duc Canh, Miaomiao Liu, Jatuwat Sangsanont, Hiroyuki Katayama
Summary: Waterborne diseases caused by pathogenic human viruses are a major public health concern. An advanced approach called capsid integrity (RT-)qPCR has been developed to assess the infectivity of pathogenic viruses. This study reviews the development and application of this approach and discusses its efficiency and potential future applications.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Joanne Hewitt, Sam Trowsdale, Bridget A. Armstrong, Joanne R. Chapman, Kirsten M. Carter, Dawn M. Croucher, Cassandra R. Trent, Rosemary E. Sim, Brent J. Gilpin
Summary: Wastewater-based epidemiology (WBE) is utilized to monitor COVID-19 infections in communities. This study estimates the required number of COVID-19 cases for detecting SARS-CoV-2 RNA in wastewater, demonstrating that WBE is a reliable and sensitive method even in low prevalence settings.
Article
Plant Sciences
Stefano Panno, Andrea Giovanni Caruso, Sofia Bertacca, Slavica Matic, Salvatore Davino, Giuseppe Parrella
Summary: Parietaria mottle virus (PMoV) is an emerging virus in the Mediterranean basin, causing confusion with other viruses on tomato and pepper crops. A sensitive RT-qPCR assay has been developed for early detection of PMoV, showing the virus is increasingly present in tomato crops in Sicily, Italy, affecting the horticultural sector negatively.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Hyuna Park, Wonjong Jung, Hyeongseok Jang, Kak Namkoong, Kwon-Young Choi
Summary: This study developed a simple and rapid thermolysis method that can bypass the need for viral RNA extraction and purification. The method integrates thermolysis, RT, and digital PCR into a single chip for large-scale viral analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Erika Corrales-Cabra, Monica Higuita, Rodrigo Hoyos, Yuliana Gallo, Mauricio Marin, Pablo Gutierrez
Summary: This study found a wide prevalence of multiple viruses in Cape gooseberry, including co-infections, and a high diversity of viruses. Urgent measures should be taken to certify the health status of planting material and crops.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Medicine, General & Internal
Huijun Zong, Shengwei Zhang, Xueyi Shang, Hua Jiang, Zhongpeng Zhao, Shaolong Chen, Xin Wang, Ye Wang, Yongqiang Jiang, Xinyu Li, Lingyun Tan, Peng Liu, Qingyu Lv, Yan Li
Summary: After optimizing the conditions, AlphaLISA showed higher sensitivity and throughput in the detection of influenza B virus, and can be used for virus diagnosis and epidemic control.
FRONTIERS IN MEDICINE
(2023)
Article
Parasitology
Jyoti S. Kumar, Manmohan Parida, Anita M. Shete, Triparna Majumdar, Savita Patil, Pragya D. Yadav, Paban Kumar Dash
Summary: A rapid and sensitive RT-LAMP assay for detection of CCHFV has been developed in this study, which can be completed in a short time, providing crucial information for outbreak control and clinical management.
Article
Veterinary Sciences
Yi-Lun Xie, Dian-Hong Lv, Xiao-Hui Wen, Qi Zhai, Man-Lin Luo, Wen-Kang Wei, Qin-Ling Chen, Shao-Lun Zhai
Summary: This study successfully developed a TaqMan-based real-time quantitative RT-PCR assay for efficient detection of bovine rhinitis B virus (BRBV), with high specificity and reproducibility. Testing of samples from cattle in China in recent years revealed a certain percentage of BRBV-positive animals, providing valuable data for epidemiological investigations of this virus.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Plant Sciences
Madalyn K. Shires, Alice A. Wright, Scott J. Harper
Summary: Little cherry virus-2 (LChV-2) is a viral pathogen that is causing an epidemic in Washington State, with significant impacts on sweet cherry production. Researchers have developed a new diagnostic assay to detect the virus and identified two distinct genotypes of LChV-2 in Washington State. The new assay has higher sensitivity and can detect the virus in different tissue types with minimal inhibition.
Correction
Virology
Enrique Rivadulla, Jesus L. Romalde
Review
Infectious Diseases
David Polo, Alberto Lema, Enia Gandara, Jesus L. Romalde
Summary: Through a systematic review and meta-analysis of 35 studies in Europe, it was found that HBoV has a moderate prevalence among European countries, with no significant differences in prevalence by country. The study also analyzed the prevalence of HBoV as a single infection or co-infection with other viruses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Chunqi Jiang, Mami Tanaka, Sayo Nishikawa, Sayaka Mino, Jesus L. Romalde, Fabiano L. Thompson, Bruno Gomez-Gil, Tomoo Sawabe
Summary: This study presents the second update of molecular phylogenetic analysis of Vibrionaceae, adding new insights into the diversity and evolutionary history of this bacterial family. The results show that the classification based on nucleotide sequences of eight housekeeping genes is reliable, and the set of 11 ribosomal protein genes is useful for identifying uncultured species among metagenome-assembled genome pools. This research contributes to taxonomic applications in classifying not-yet-cultured taxa and expanding our understanding of Vibrionaceae.
CURRENT MICROBIOLOGY
(2022)
Article
Virology
Maria Soderlund-Venermo, Anupam Varma, Deyin Guo, Douglas P. Gladue, Emma Poole, Flor H. Pujol, Hanu Pappu, Jesus L. Romalde, Laura Kramer, Mariana Baz, Marietjie Venter, Matthew D. Moore, Michael M. Nevels, Sayeh Ezzikouri, Vikram N. Vakharia, William C. Wilson, Yashpal S. Malik, Zhengli Shi, Ahmed S. Abdel-Moneim
Summary: This article summarizes the presentations given at the 1st international conference of the World Society for Virology (WSV). The conference focused on tackling global viral epidemics and aimed to foster international collaborations. The conference consisted of multiple sessions and attracted 270 scientists from 49 countries.
Article
Public, Environmental & Occupational Health
Nasreen Hassoun-Kheir, Yoav Stabholz, Jan-Ulrich Kreft, Roberto de la Cruz, Arnaud Dechesne, Barth F. Smets, Jesus L. Romalde, Alberto Lema, Sabela Balboa, Carlos Garcia-Riestra, Eva Torres-Sangiao, Ami Neuberger, David Graham, Marcos Quintela-Baluja, Dov J. Stekel, Jay Graham, Amy Pruden, Joseph Nesme, Soren Johannes Sorensen, Rupert Hough, Mical Paul
Summary: By utilizing a systematic review and Delphi consensus method, the EMBRACE-WATERS statement was developed as a checklist of recommendations for reporting studies of AMR in wastewater and related aquatic environments. The consensus among a multidisciplinary panel of experts resulted in 21 items comprising essential elements for high-quality reporting, with explanations and reporting examples provided for each item. The statement aims to improve reporting quality, facilitate communication among researchers, and guide peer-reviewers and editors in evaluating manuscripts in the field of AMR research in aquatic environments.
Article
Food Science & Technology
Soren Saxmose Nielsen, Dominique Joseph Bicout, Paolo Calistri, Elisabetta Canali, Julian Ashley Drewe, Bruno Garin-Bastuji, Jose Luis Gonzales Rojas, Christian Gortazar Schmidt, Mette Herskin, Virginie Michel, Miguel Angel Miranda Chueca, Barbara Padalino, Paolo Pasquali, Helen Clare Roberts, Hans Spoolder, Karl Stahl, Antonio Velarde, Arvo Viltrop, Christoph Winckler, Jeroen Dewulf, Luca Guardabassi, Friederike Hilbert, Rodolphe Mader, Jesus L. Romalde, Peter Smith, Francesca Baldinelli, Lisa Kohnle, Julio Alvarez
Summary: This article evaluates antimicrobial-resistant bacteria that pose a threat to the health of kept fish species and provides an overview of the global status of resistance in certain bacteria. Based on limited scientific evidence, no specific bacteria were identified as the most relevant antimicrobial-resistant species in the EU's kept fish species.
Article
Microbiology
Pablo Santibanez, Jesus Romalde, Derie Fuentes, Antonio Figueras, Jaime Figueroa
Summary: This study focused on the health status of Mytilus chilensis in Chile, revealing a good health status with no significant lesions or presence of mandatory notification pathogens. The importance of an integrated vision of different disciplines for the sustainability of the mussel industry in Chile was highlighted.
Article
Marine & Freshwater Biology
Diego Gerpe, Aide Lasa, Alberto Lema, Sabela Balboa, Jesus L. Romalde
Summary: The study of the microbiota associated with clams is important for understanding their health status and preventing pathobiology events. This study used various microbiological techniques to analyze two clam species in northwest Spain, revealing a rich bacterial diversity and isolating new bacterial taxa.
Article
Microbiology
Chunqi Jiang, Hisae Kasai, Sayaka Mino, Jesus L. Romalde, Tomoo Sawabe
Summary: In this study, the complete genome sequences of all type strains in the Splendidus clade were obtained and analyzed. The results showed that these strains have different genome sizes and plasmid numbers, which may be related to their environmental adaptation and pathogenicity. Furthermore, a broad prediction of virulence factors revealed core and species unique VF genes in the Splendidus clade.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Marcos Quintela-Baluja, Kelly Jobling, David W. Graham, Mariano Gomez-Lopez, Jesus L. Romalde, Jorge Barros-Velazquez, Pilar Calo-Mata
Summary: Raoultella ornithinolytica, an increasingly important pathogen in human diseases, was isolated from the effluent of a domestic wastewater treatment plant in Spain. The nearly complete genome sequence of a multidrug-resistant strain, R. ornithinolytica MQB_Silv_108, was reported. Its release into the environment poses a possible exposure risk for humans and animals.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Fisheries
Muhammed Duman, Noemi Bujan, Soner Altun, Jesus L. Romalde, Izzet Burcin Saticioglu
Summary: This study characterized Vibrio isolates from Turkish fish farms, PubMLST database, and GenBank using multilocus sequence analysis and typing. They found high diversity and genetic interactions within the Vibrio population. 17 new sequence types were discovered, indicating frequent genetic recombination. These findings help understand differences in Vibrio infections in fish and provide insights for effective measures in aquaculture.
Article
Fisheries
Carla Fernandez-Espinel, Marco Medina-Morillo, Rute Irgang, Giovanna Sotil, Henry Araya-Leon, Violeta Flores-Dominick, Jesus L. Romalde, Ruben Avendano-Herrera, Jefferson Yunis-Aguinaga
Summary: This study aimed to identify and classify Yersinia ruckeri isolates from the Puno Region in Peru. Two biotypes were found among the isolates, but they shared identical fingerprints and serotypes. This information is important for the epidemiological research and vaccine development against Y. ruckeri.
JOURNAL OF FISH DISEASES
(2023)
Article
Multidisciplinary Sciences
Ryota Yamano, Juanwen Yu, Alfabetian Harjuno Condro Haditomo, Chunqi Jiang, Sayaka Mino, Jesus L. Romalde, Kyuhee Kang, Yuichi Sakai, Tomoo Sawabe
Summary: This study isolated a Gram-negative, aerobic bacterium named PTE2(T) from laboratory-reared Japanese sea cucumber larvae. Phylogenetic analysis revealed that PTE2(T) was closely related to Thalassotalea sediminis N211(T), but had some differences, indicating the novelty of this strain. Based on genome-based taxonomic approaches, PTE2(T) is proposed as a new species, Thalassotalea hakodatensis sp. nov.
Article
Multidisciplinary Sciences
Rika Kudo, Ryota Yamano, Juanwen Yu, Shotaro Koike, Alfabetian Harjuno Condro Haditomo, Mayanne A. M. de Freitas, Jiro Tsuchiya, Sayaka Mino, Fabiano Thompson, Jesus L. Romalde, Hisae Kasai, Yuichi Sakai, Tomoo Sawabe
Summary: Strain PT1(T), a rod-shaped bacterium isolated from laboratory-reared larvae of sea cucumber Apostichopus japonicus, demonstrated similarities to Neptuniibacter marinus ATR 1.1(T) and Neptuniibacter caesariensis MED92(T) with sequence similarities of 98.2% and 98.1%, respectively, based on the analysis of 16S rRNA gene nucleotide sequences. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were below 95% and 70%, confirming the novel status of PT1(T). Based on the genomic approach, a new species, Neptuniibacter victor sp. nov., is proposed for PT1(T).
Article
Fisheries
Jose R. Lopez, Jesus L. Romalde, Montserrat Lopez-Sanmartin, Jose I. Navas
Summary: In this study, a disease outbreak in meagre caused by Vibrio tapetis was investigated. The mortality rate reached 45% within one week. The bacteria were recovered from the liver and kidney of the affected fish, and further characterization confirmed their pathogenicity in sea bass.
