Article
Agronomy
Hanna Kronfeld, Nicole Kemper, Christina S. Hoelzel
Summary: Knowledge of vaginal and uterine microbiomes is crucial for preventing uterine diseases and understanding their development. A study on dairy cows found that cows with pathological puerperium had reduced bacterial diversity and higher occurrence of certain bacterial genera (such as Fusobacteria spp., Bacteroides spp., and Helcococcus spp.). The study also observed correlation between bacterial occurrences in the vagina and uterus, and a lower incidence of coagulase-negative staphylococci in cows with puerperal disorders.
Article
Agriculture, Dairy & Animal Science
Josiane C. C. Silva, Leonardo Bringhenti, Lucas C. Siqueira, Marjory X. Rodrigues, Martin Zinicola, Brianna Pomeroy, Rodrigo C. Bicalho
Summary: This study investigated whether a metritis induction model would induce disease in postpartum primiparous cows. The results showed that the incidence of metritis did not differ among groups, but the high-dose group had a greater hazard of being diagnosed with metritis and had reduced milk production. The bacterial challenge affected milk production and was associated with an increase in bacterial counts of Fusobacterium necrophorum.
Article
Agriculture, Dairy & Animal Science
J. C. C. Silva, L. C. Siqueira, M. X. Rodrigues, M. Zinicola, P. Wolkmer, B. Pomeroy, R. C. Bicalho
Summary: Metritis is a common disorder in dairy cattle caused by bacterial infection, with a higher incidence in the early postpartum period. An in vivo model of clinical metritis was developed using a pathogenic cocktail of bacteria in Holstein multiparous cows. A lower dose of bacteria resulted in a greater incidence of metritis, lesser dry matter intake, and an acute phase protein profile typifying metritis. These findings create a relevant model for the study of uterine diseases in postpartum cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Immunology
Xin Shen, Lin Xu, Zhiquan Zhang, Yitong Yang, Pengxian Li, Teng Ma, Shuai Guo, Lai-Yu Kwok, Zhihong Sun
Summary: Vaginitis is a common gynecological disease caused by vaginal dysbiosis. Clinical treatment using antibiotics often results in adverse reactions and relapse, while probiotics intervention can restore vaginal microbiota and alleviate bacterial vaginitis (BV). In this study, a clinical trial involving 50 BV patients was conducted to evaluate the efficacy of a postbiotic gel applied for one week. The results showed that the postbiotic gel improved symptoms of BV and increased the abundance of vaginal lactobacilli, which were negatively correlated with potential vaginal pathogens and abnormal vaginal secretions. Overall, this study provides valuable clinical data for managing BV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Microbiology
Yiwen Han, Zhaoxia Liu, Tingtao Chen
Summary: Vaginal microbiota dysbiosis plays a significant role in gynecological infectious and non-infectious diseases, and intervention on microbiota composition is crucial for the treatment of gynecological diseases.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Kwan Young Oh, Sunghee Lee, Myung-Shin Lee, Myung-Ju Lee, Eunjung Shim, Yun Ha Hwang, Joong Gyu Ha, Yun Seok Yang, In Taek Hwang, Jun Sook Park
Summary: This study investigated the vaginal microbiota of pregnant women with aerobic vaginitis (AV), finding lower levels of Lactobacillus crispatus in the AV groups compared to normal flora, while other species were higher. The decrease in L. crispatus relative abundance was common across multiple criteria of the AV scoring system, suggesting a potential influence on AV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Reproductive Biology
Edoardo Lillo, Marco Cordisco, Adriana Trotta, Grazia Greco, Alice Carbonari, Annalisa Rizzo, Raffaele Luigi Sciorsci, Marialaura Corrente
Summary: This study aimed to test the bactericidal action of an Ozone/Oxygen gas mixture on bacteria isolated from the cervico-vaginal mucus of cows affected by acute metritis. The results showed that the Ozone/Oxygen gas mixture can effectively inhibit and kill the pathogenic bacteria isolated from the cervico-vaginal mucus of cows with acute metritis.
Article
Immunology
David Pacha-Herrera, Maria P. Erazo-Garcia, Dario F. Cueva, Miguel Orellana, Pamela Borja-Serrano, Camila Arboleda, Eduardo Tejera, Antonio Machado
Summary: The vaginal microbiota plays a crucial role in protecting women, with the probiotic activity provided by individual lactobacillus species and their multi-microbial interactions. This study analyzed the prevalence of five vaginal lactobacilli among healthy women and those with bacterial vaginosis (BV) or aerobic vaginitis (AV). The findings showed that Lactobacillus acidophilus, Lactobacillus jensenii, and Lactobacillus crispatus had the highest normalized importance values in protecting against vaginal dysbiosis, with L. acidophilus and L. jensenii exhibiting statistical significance. Additionally, a strong multi-microbial consortium of L. iners, L. jensenii, L. gasseri, and L. acidophilus was found to protect against AV and BV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Giuseppina Campisciano, Nunzia Zanotta, Vincenzo Petix, Manuela Giangreco, Giuseppe Ricci, Gianpaolo Maso, Manola Comar, Francesco De Seta
Summary: The translation discusses the diagnosis of bacterial vaginosis using Nugent score and qPCR, with qPCR proving to be more informative and accurate. Results showed discordance between Gram staining and qPCR in cases of partial BV, with a significant association between STIs and partial BV.
Article
Microbiology
Qian Wang, Ang Dong, Jinshuai Zhao, Chen Wang, Christipher Griffin, Claudia Gragnoli, Fengxia Xue, Rongling Wu
Summary: This study uses functional graph theory, combining allometry scaling law and evolutionary game theory, to reveal the bidirectional, signed, and weighted interactions between microbes in microbial communities, and finds that the role of Lactobacillus in maintaining vaginal microbial symbiosis is enabled by upregulation from other microbes.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Adriana Garzon, Gregory Habing, Fabio Lima, Noelia Silva-Del-Rio, Festus Samah, Richard Pereira
Summary: Puerperal metritis (PM) is a common infectious disease in dairy cattle, but there are discrepancies in clinical case definitions and practices. The lack of consensus in terminology and standards for defining PM cases can result in differences in recommendations for treating cows and the use of antimicrobials.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
T. V. Silva, E. B. de Oliveira, J. Perez-Baez, C. A. Risco, R. C. Chebel, F. Cunha, R. Daetz, J. E. P. Santos, F. S. Lima, K. C. Jeong, K. N. Galvao
Summary: Treating dairy cows with metritis using ceftiofur-free acid resulted in higher treatment costs but increased pregnancy rates and decreased culling, leading to improved profitability compared to leaving them untreated. Metritis caused economic losses, but treating with ceftiofur reduced losses by improving fertility, reducing culling and replacement costs, and minimizing milk yield losses.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
P. R. Menta, R. C. Neves, V. S. Machado
Summary: The association of early and late metritis diagnosis with energy metabolites, minerals, and haptoglobin concentrations in postpartum Jersey cows was investigated. Blood samples were collected from 379 cows and analyzed for various biomarkers. Cows diagnosed with early and late metritis had higher concentrations of albumin, & beta;-hydroxybutyrate, and haptoglobin compared to cows without metritis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Rositsa Mileva, Manol Karadaev, Ivan Fasulkov, Nikolina Rusenova, Nasko Vasilev, Aneliya Milanova
Summary: This study evaluated the changes in oxytetracycline levels in uterine secretion over time in cows with metritis, and its association with the sensitivity of pathogenic bacteria. The results showed that the intrauterine infusion of oxytetracycline for three consecutive days successfully treated metritis and restored the estrus cycle in cows.
Article
Microbiology
Samat Amat, Devin B. Holman, Kaycie Schmidt, Ana Clara B. Menezes, Friederike Baumgaertner, Thomas Winders, James D. Kirsch, Tingting Liu, Timothy D. Schwinghamer, Kevin K. Sedivec, Carl R. Dahlen
Summary: The study evaluated the impact of maternal restricted gain and VTM supplementation on the nasopharyngeal, ruminal, and vaginal microbiota of beef heifers. The results showed that there were no significant differences in microbiota diversity due to maternal weight gain during the first trimester or VTM supplementation during pregnancy. However, core taxa were identified across all three microbiota, including methanogenic archaea.
Article
Biotechnology & Applied Microbiology
Klibs N. Galvao, Eduardo B. de Oliveira, Federico Cunha, Rodolfo Daetz, Kristi Jones, Zhengxin Ma, Kwangcheol C. Jeong, Rodrigo C. Bicalho, Catherine H. Higgins, Marjory X. Rodrigues, Candelaria Gonzalez Moreno, Soojin Jeon
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Engineering, Environmental
F. Bandini, A. Frache, A. Ferrarini, E. Taskin, P. S. Cocconcelli, Edoardo Puglisi
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2020)
Article
Food Science & Technology
Kostas Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan J. Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis N. Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto, Amparo Querol, Juan Evaristo Suarez, Ingvar Sundh, Just M. Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman
Article
Biotechnology & Applied Microbiology
Vania Patrone, Tahani Al-Surrayai, Francesco Romaniello, Alessandra Fontana, Giovanni Milani, Valeria Sagheddu, Edoardo Puglisi, Maria Luisa Callegari, Hamad Al-Mansour, Mohamed Waheed Kishk, Lorenzo Morelli
Summary: Probiotics, especially the Weissella genus, show potential for controlling intestinal infections in livestock. Two Weissella cibaria strains isolated from cows in Kuwait exhibited strong growth under acidic conditions and bile salts, along with adhesion properties to host cells and inhibitory effects on E. coli and S. enterica due to their low pH supernatants. Genetic analysis revealed genes related to metabolism and stress responses, indicating adaptation to the gastrointestinal environment. Strain SP19 showed promising probiotic properties and genetic markers, making it a candidate for further investigation in livestock use.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Multidisciplinary Sciences
Francesco Miragoli, Vania Patrone, Aldo Prandini, Samantha Sigolo, Matteo Dell'Anno, Luciana Rossi, Mario Barbato, Alice Senizza, Lorenzo Morelli, Maria Luisa Callegari
Summary: The study demonstrates that supplementing tannins can modulate the gut microbiota of piglets, promoting the growth of butyrate-producing bacteria and altering the composition of gut microbiota.
Article
Microbiology
Francesco Miragoli, Vania Patrone, Aldo Prandini, Samantha Sigolo, Matteo Dell'Anno, Luciana Rossi, Alice Senizza, Lorenzo Morelli, Maria Luisa Callegari
Summary: The addition of tributyrin to piglets' basal diet at weaning can significantly alter the composition of their gut microbiota, increasing the abundance of beneficial bacterial genera. This can improve animal growth performance and influence the functional potential of the gut microbiome.
Article
Food Science & Technology
Elisabetta Margiotta, Lara Caldiroli, Maria Luisa Callegari, Francesco Miragoli, Francesca Zanoni, Silvia Armelloni, Vittoria Rizzo, Piergiorgio Messa, Simone Vettoretti
Summary: In elderly CKD patients, sarcopenic individuals had differences in gut microbiota composition compared to non-sarcopenic ones. While several bacterial families and genera were associated with uremic toxins and inflammatory cytokines, these factors did not substantially differ between sarcopenic and non-sarcopenic patients.
Article
Agriculture, Dairy & Animal Science
Matteo Dell'Anno, Serena Reggi, Valentina Caprarulo, Monika Hejna, Carlo Angelo Sgoifo Rossi, Maria Luisa Callegari, Antonella Baldi, Luciana Rossi
Summary: The study assessed the effects of feeding a combination of tannin extracts, leonardite, and tributyrin on weaned piglets, showing that the mixture could reduce diarrhoea incidence, improve faecal consistency, and modulate serum metabolism.
Article
Agriculture, Dairy & Animal Science
Matteo Dell'Anno, Maria Luisa Callegari, Serena Reggi, Valentina Caprarulo, Carlotta Giromini, Ambra Spalletta, Simona Coranelli, Carlo Angelo Sgoifo Rossi, Luciana Rossi
Summary: This study assessed the effects of Lactobacillus plantarum and Lactobacillus reuteri on in-feed supplementation in weaned piglets, showing that these probiotics can effectively reduce diarrhoea occurrence and serve as interesting functional additives to prevent antibiotic overuse.
Article
Microbiology
Paolo Bellassi, Alessandra Fontana, Maria Luisa Callegari, Fabrizio Cappa, Lorenzo Morelli
Summary: The novel bacterium 2CT exhibits characteristics of a newly proposed Microbacterium species, showing optimal growth conditions in terms of salt tolerance, temperature, and pH range. Phylogenetic analysis based on concatenated 16S rRNA and four housekeeping genes confirmed 2CT to be a new species within the Microbacterium genus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Andrea Torres Luque, Cecilia Fontana, Sergio E. Pasteris, Daniela Bassi, Pier S. Cocconcelli, Maria C. Otero
Summary: The study identified differences in vaginal microbiota composition and diversity between gilts and pregnant sows, showing lower richness and higher relative abundance of Proteobacteria in pregnant sows. Additionally, the microbial populations differed between sows based on breeding method, with artificial insemination leading to lower levels of Firmicutes compared to natural mating sows.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Environmental Sciences
Laura Pellegrinelli, Sara Castiglioni, Clementina Cocuzza, Barbara Bertasi, Valeria Primache, Silvia Schiarea, Giulia Salmoiraghi, Andrea Franzetti, Rosario Musumeci, Michela Tilola, Elisa Galuppini, Giorgio Bertanza, Marialuisa Callegari, Fabrizio Stefani, Andrea Turolla, Emanuela Ammoni, Danilo Cereda, Elena Pariani, Sandro Binda
Summary: This study evaluated the performance of different pre-analytical and analytical methods for identifying the presence of SARS-CoV-2 in untreated municipal wastewater samples. It concluded that PEG-8000 precipitation combined with real-time RT-PCR targeting the N gene was the best workflow for detecting SARS-CoV-2 RNA in wastewater.
Article
Food Science & Technology
Sara Zambianchi, Vania Patrone, Pier Paolo Becchi, Maria Luisa Callegari, Lorenzo Stagnati, Luigi Lucini, Lorenzo Morelli, Matteo Busconi
Summary: Buttafuoco dell'Oltrepo' Pavese is an important and renowned red wine protected by a Denominazione di Origine Controllata (DOC) designation. The study investigates microbial populations and metabolomic composition of the wine, revealing the impact of sampling time on microbial terroir and the presence of discriminating molecules related to different edaphic conditions.
Article
Food Science & Technology
Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Birgit Dusemund, Mojca Kos Durjava, Maryline Kouba, Marta Lopez-Alonso, Secundino Lopez Puente, Francesca Marcon, Baltasar Mayo, Alena Pechova, Mariana Petkova, Fernando Ramos, Yolanda Sanz, Roberto Edoardo Villa, Ruud Woutersen, Pier Sandro Cocconcelli, Boet Glandorf, Lieve Herman, Miguel Prieto Maradona, Maria Saarela, Montserrat Anguita, Jaume Galobart, Orsolya Holczknecht, Paola Manini, Elisa Pettenati, Fabiola Pizzo, Jordi Tarres-Call
Article
Food Science & Technology
Kostas Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman