Article
Biochemistry & Molecular Biology
Georgios Vasilopoulos, Roman Moser, Jonas Petersen, Meriyem Aktas, Franz Narberhaus
Summary: Bacterial membranes are primarily composed of phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CL). Some bacteria also produce phosphatidylcholine (PC), the hallmark of eukaryotic membranes. The biosynthesis pathways for PC and CL in bacteria have been studied, revealing enzyme activities involved in these processes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Physics, Fluids & Plasmas
Ion Santra, Urna Basu, Sanjib Sabhapandit
Summary: This study investigates four distinct dynamical regimes of active Brownian motion in two dimensions, characterized by the presence of two timescales set by the rotational diffusion constant and the reversal rate. Analytical computation of position distribution and persistence exponents reveals that in regime II, the position distribution orthogonal to the initial orientation can be described using a scaled variable, showing nontrivial scaling behavior with a special scaling function. The exact first-passage time distribution computation demonstrates the emergence of a persistence exponent of 1 due to direction reversal in this regime.
Article
Microbiology
Rim Werheni Ammeri, Yassine Hidri, Wafa Hassen, Ines Mehri, Nesrine Khlifi, Abdennaceur Hassen
Summary: The study evaluated the effect of three surfactants on the removal of PCP by Pseudomonas putida. Results showed that SDS had the most significant impact on bacterial growth and PCP removal, with 95% degradation in mineral salt medium. Additionally, chloride concentration was found to have a significant relation with PCP removal.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Environmental Sciences
Hua Li, Arnaud Dechesne, Zhiming He, Marlene Mark Jensen, Hai Liang Song, Barth F. Smets
Summary: Current in the milliampere range can enhance conjugal plasmid transfer between bacteria, including antibiotic-resistant bacteria. The exposure to sublethal current intensities during electrochemical disinfection can lead to increased transconjugant frequency and expression of conjugation-related genes. It is crucial to carefully control the conditions of electrochemical disinfection to prevent the transmission of antibiotic resistance genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Shreya Ghosh, Anam Ejaz, Lucas Repeta, Stewart Shuman
Summary: The study identifies Pseudomonas putida MPE as a novel DNA endonuclease capable of sensing and cutting double-stranded DNA, and hydrolyzing the single-stranded DNA backbone in different orientations to generate distinct cleavage products.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Ecology
Xinzhu Yi, Jie-Liang Liang, Jian-Qiang Su, Pu Jia, Jing-li Lu, Jin Zheng, Zhang Wang, Shi-wei Feng, Zhen-hao Luo, Hong-xia Ai, Bin Liao, Wen-sheng Shu, Jin-tian Li, Yong-Guan Zhu
Summary: Mining activities have significant environmental impacts and can co-select for antibiotic resistance genes (ARGs) in microorganisms. However, ARGs in mining-impacted environments are poorly understood. In this study, a comprehensive investigation of ARGs in globally distributed mining sites was conducted. The results showed that the total abundance of ARGs in these sites was comparable to urban sewage but higher than freshwater sediments. Multidrug resistance genes accounted for a substantial portion of the total ARG abundance and tended to co-occur with multimetal resistance genes. Additionally, high-quality ARG-carrying genomes were identified, expanding the phylogenetic diversity of ARG hosts and revealing potential pathogens. These findings suggest that mining-impacted environments are important reservoirs of multidrug resistance genes.
Article
Biotechnology & Applied Microbiology
Hemanoel Passarelli-Araujo, Sarah H. Jacobs, Gloria R. Franco, Thiago M. Venancio
Summary: P. alloputida was proposed as a new species and its population structure includes at least 7 clonal complexes. Clinical isolates are mainly found in one of the complexes, and horizontal gene transfer plays an important role in shaping its ability to bioremediate aromatic compounds.
Review
Biochemical Research Methods
Shawn Kulakowski, Deepanwita Banerjee, Corinne D. Scown, Aindrila Mukhopadhyay
Summary: Microbial bioconversion is an important approach to produce sustainable chemicals and commodities. However, in industrial-scale bioproduction, anaerobic conditions are preferred due to the high cost of oxygen transfer. Anaerobic bioconversion usually has limited substrate utilization, lower product yields, and reduced final product diversity compared to aerobic processes. This study introduces advances in microbial strain engineering, such as redox cofactor engineering, genome-scale metabolic modeling, and functional genomics, to improve bioproduction processes under low oxygen conditions and enable industrial-scale bioproduction systems.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sergi Burguera, Antonio Frontera, Antonio Bauza
Summary: By combining computations and X-ray structure analysis, we have demonstrated that [Pt(CN4)](2-) can act as a Lewis acid inside an enzyme's cavity. The nature of an unexpected contact between a catalytically active GLN residue in a mitochondrial synthase and the Pt(ii) center was investigated using molecular dynamics and quantum mechanics calculations. The results confirm the electron acceptor role of [Pt(CN4)](2-), which serves as inspiration for designing biomolecular cages that can modify the nucleophilic/electrophilic character of an organometallic compound.
CHEMICAL COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Alexandra Stoll, Ricardo Salvatierra-Martinez, Maximo Gonzalez, Jonathan Cisternas, Angela Rodriguez, Antonio Vega-Galvez, Jaime Bravo
Summary: Incorporating beneficial microorganisms into agriculture crop management has become common practice in recent decades. Through experiments on different application strategies, it has been shown that early application of microbial inoculants during crop phenological stages like nurseries can significantly improve plant survival rate and productivity. Timing of PGPR inoculants application plays a crucial role in sustainable agriculture and product development.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Francisco Rios, Manuela Lechuga, Ismael Lobato-Guarnido, Mercedes Fernandez-Serrano
Summary: The aim of this study is to assess the toxicological effects of three anionic surfactants and three amphoteric surfactants, individually and in binary mixtures, on bacteria and marine microalgae. The results showed that the marine microalgae were more sensitive to the surfactants and mixtures, and the toxic effects of the mixtures were lower than expected.
Article
Biotechnology & Applied Microbiology
Cholpisit Kiattisewee, Chen Dong, Jason Fontana, Widianti Sugianto, Pamela Peralta-Yahya, James M. Carothers, Jesse G. Zalatan
Summary: This study successfully transferred the CRISPRa system to P. putida by optimizing the expression levels of CRISPRa system components and applying rules based on systematic characterization of promoter features, enabling regulation of biosynthesis in the biopterin and mevalonate pathways. Multiple genes can be activated simultaneously by targeting multiple promoters or a single promoter in a multi-gene operon, paving the way for new metabolic engineering strategies in P. putida and other bacterial species.
METABOLIC ENGINEERING
(2021)
Article
Engineering, Environmental
Xiaoqing Meng, Hua Li, Su Yan, Zengping Ning, Quan Yuan, Chengshuai Liu, Lixiang Zhou, Guanyu Zheng
Summary: The present study found that pre-acidification greatly promoted the inactivation of a multi-drug resistant bacterium and the degradation of carried antibiotic resistance genes (ARGs) in wastewater. Pre-acidification led to cell lysis and the release of ARGs, which were then degraded by the generated radicals. This study highlights the importance of efficiently lysing ARB cells to promote the degradation of carried ARGs and reduce antibiotic resistance in wastewater.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Emmanuel Oliver Fenibo, Ramganesh Selvarajan, Akebe Luther King Abia, Tonderayi Matambo
Summary: Hydrocarbon footprints in the environment affect the ecosystem and result in imbalances that lead to human diseases and reduced biodiversity. Microbial bioremediation using functional genes, such as the bacterial alkB gene, is a sustainable option for cleaning hydrocarbon-impacted environments. However, relying solely on alkB detection as a monitoring parameter for hydrocarbon degradation is erroneous due to its distribution among bacteria and limitations in linking copy number to contamination concentration levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Chemistry, Physical
Carolina F. Jesus, Andreia A. S. Alves, Sonia M. Fiuza, Dina Murtinho, Filipe E. Antunes
Summary: Surfactants play a vital role in daily life, but the environmental and health concerns associated with increased production have led to a search for sustainable alternatives. Sugar-based surfactants, especially cationic ones, show promising properties and applications, making them a potential solution to the current challenges.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biotechnology & Applied Microbiology
Maria F. Bergero, Gloria I. Lucchesi
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2015)
Article
Microbiology
Romina Marisa Heredia, Paola Sabrina Boeris, Andres Sebastian Liffourrena, Maria Fernanda Bergero, Gaston Alberto Lopez, Gloria Ines Lucchesi
Article
Microbiology
Mariana L. Puente, Jose L. Gualpa, Gaston A. Lopez, Romina M. Molina, Susana M. Carletti, Fabricio D. Cassan
Article
Microbiology
Romina Molina, Diego Rivera, Veronica Mora, Gaston Lopez, Susana Rosas, Stijn Spaepen, Jos Vanderleyden, Fabricio Cassan
CURRENT MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
D. Rivera, V. Mora, G. Lopez, S. Rosas, S. Spaepen, J. Vanderleyden, F. Cassan
JOURNAL OF APPLIED MICROBIOLOGY
(2018)
Article
Microbiology
Romina Marisa Heredia, Paola Sabrina Boeris, Maria Alicia Biasutti, Gaston Alberto Lopez, Natalia Soledad Paulucci, Gloria Ines Lucchesi
Article
Microbiology
Molina Romina, Lopez Gaston, Rodriguez Belen, Rosas Susana, Mora Veronica, Cassan Fabricio
ARCHIVES OF MICROBIOLOGY
(2020)
Review
Soil Science
Fabricio Cassan, Anahi Coniglio, Gaston Lopez, Romina Molina, Sofia Nievas, Coline Le Noir de Carlan, Florencia Donadio, Daniela Torres, Susana Rosas, Fabio Olivera Pedrosa, Emanuel de Souza, Martin Diaz Zorita, Luz de-Bashan, Veronica Mora
BIOLOGY AND FERTILITY OF SOILS
(2020)
Article
Ecology
Fabricio Cassan, Gaston Lopez, Sofia Nievas, Anahi Coniglio, Daniela Torres, Florencia Donadio, Romina Molina, Veronica Mora
Summary: Azospirillum is a successful genus of plant growth-promoting bacteria and a study model for plant-bacteria interactions. A wide range of topics have been discussed in publications over the past four decades, with researchers analyzing details about the work done with Azospirillum since its discovery in 1925. This has led to a linear increase in the number of published works, particularly focusing on the mechanisms involved in promoting growth in various plant species.
Article
Biotechnology & Applied Microbiology
R. Molina, G. Lopez, A. Coniglio, A. Furlan, V Mora, S. Rosas, F. Cassan
Summary: The study evaluated the effects of light on growth, cell physiology, and stress response of non-photosynthetic rhizobacteria Azospirillum brasilense Az39 under planktonic growth conditions. Blue light and daylight were found to be stress effectors, affecting biomass production, IAA biosynthesis, bacterial response to stress, cell aggregation, and biofilm formation differently. These findings could be useful in improving the production and functionality of biofertilizers.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Daniela Torres, Elias Mongiardini, Florencia Donadio, Raul Donoso, Gonzalo Recabarren-Gajardo, Jose Gualpa, Stijn Spaepen, Roberto Defez, Gaston Lopez, Carmen Bianco, Fabricio Cassan
Summary: The bacterium Bradyrhizobium japonicum E109 is known for its ability to degrade indole-3-acetic acid (IAA) and the genome analysis revealed genes related to IAA synthesis. Two sequences responsible for IAA degradation were identified, along with the identification of two degradation products.
RESEARCH IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Daniela Torres, Florencia Donadio, Gaston Lopez, Romina Molina, Melissa Obando, Sofia Nievas, Susana Rosas, Sanja Cavar Zeljkovic, Martin Diaz-Zorita, Nuria De Diego, Fabricio Cassan
Summary: This study evaluated the previous incubation between BjE109 and AaAz39 and its impact on Bradyrhizobium-soybean symbiosis and plant growth. The results showed that the previous incubation improved the survival of BjE109 on soybean seeds and increased nodule number, size, biomass, and nodulation percentage. The grain yield under field conditions also significantly increased compared to single inoculation or immediate combination of both strains.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)