Article
Multidisciplinary Sciences
Hanli Guo, Weifeng Yin, Ziling Zou, Chao Zhang, Minghui Sun, Lingtian Min, Lei Yang, Lingyi Kong
Summary: The study found that quercitrin promoted cell proliferation and delayed extracellular matrix degradation by regulating MMP13 and collagen II gene and protein expressions. Additionally, quercitrin activated the Phosphatidylinositol 3-kinase p110a (p110a)/AKT/mTOR signaling pathway by targeting p110a.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Biology
Ana Bura, Antonija Jurak Begonja
Summary: Phosphoinositides play crucial roles in cells with limited understanding of their localization and functions in platelets. This study investigated the localization of PI(4,5)P-2 and PI4P in resting and activated platelets through antibody staining. The intracellular pools of PI(4,5)P-2 and PI4P in platelets can be modulated by inhibitors of OCRL phosphatase and PI4KIII alpha kinase, with a more sensitive response in activated platelets.
Review
Cell Biology
Ana Bura, Sara Cabrijan, Iris Duric, Tea Bruketa, Antonija Jurak Begonja
Summary: Phosphoinositides (PIs) are phosphorylated lipids that have diverse cellular functions including regulation of trafficking, actin reorganization, and cell signaling. The two most abundant PIs in cells are phosphatidylinositol4-monophosphate (PI4P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], which localize to different cellular compartments and regulate various processes. The levels of PIs are controlled by multiple kinases and phosphatases, and understanding their localization and function is important for studying cellular processes.
Article
Cell Biology
Martin Sztacho, Barbora Salovska, Jakub Cervenka, Can Balaban, Peter Hoboth, Pavel Hozak
Summary: Analysis of nuclear protein interactions with phosphoinositides reveals the role of PIP2 in regulating gene expression, RNA splicing, and cell cycle processes. The identified proteins are involved in various functions within the nucleus, with different PIP2-binding motifs influencing their localization and activity. This study provides insights into the molecular mechanism of nuclear PIP2 protein interaction and offers a methodology for further research on PIPs and other protein ligands.
Article
Immunology
Yulan Huang, Shitong He, Yitian Chen, Junli Sheng, Yuling Fu, Xialin Du, Yalong Yang, Honglin Liu, Zhenyu Han, Yingqi Huang, Qian Wen, Chaoying Zhou, Xinying Zhou, Shengfeng Hu, Li Ma
Summary: In this study, we identified UCHL1 as a positive regulator of M1 macrophage polarization. Our findings may help in developing therapeutic interventions for the treatment of inflammatory diseases and pathogenic infections.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Meredith L. Jenkins, Harish Ranga-Prasad, Matthew A. H. Parson, Noah J. Harris, Manoj K. Rathinaswamy, John E. Burke
Summary: The PIK3CA gene is frequently mutated in human cancer, and this study reveals the molecular mechanisms underlying the increased activity mediated by different oncogenic mutations in PIK3CA. Through a combination of biochemical assays and HDX-MS, the authors uncover unique regulatory mechanisms and explain how these mutations activate PI3K. This work has important implications for the development of mutant selective inhibitors.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Katerina Cizkova, Katerina Koubova, Zdenek Tauber
Summary: This study revealed that activators and inhibitors of PPAR alpha affect the PI3K/Akt/PTEN pathway in intestinal cell differentiation, highlighting their impact on brush border formation.
Article
Biochemistry & Molecular Biology
Yong Shi, Anne Berking, Timo Baade, Kyle R. Legate, Reinhard Fassler, Christof R. Hauck
Summary: Staphylococcus aureus invades host cells mainly through an integrin-dependent manner, with phosphatidylinositol-4,5-bisphosphate (PI-4,5-P-2) playing a significant role in regulating integrin-associated proteins. Local synthesis of PI-4,5-P-2 by a focal adhesion-associated lipid kinase is crucial for integrin-mediated internalization of S. aureus. Targeting active PI-4,5-P-2 phosphatase to the plasma membrane and specific ablation of talin and FAK-binding motif in PIP5KI gamma 90 can reduce bacterial invasion.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Multidisciplinary Sciences
Veronika Thallmair, Lea Schultz, Wencai Zhao, Siewert J. Marrink, Dominik Oliver, Sebastian Thallmair
Summary: Phosphoinositides (PIs), especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2], play a crucial role in targeting proteins to cilia. This study discovered that the tubby domain, which is essential for this targeting process, binds to PI(4,5)P-2 through two binding sites.
Article
Physiology
Junlan Zhou, Neha Singh, Chloe Monnier, William Marszalec, Li Gao, Jing Jin, Michael Frisk, William E. Louch, Suresh Verma, Prasanna Krishnamurthy, Elsa Nico, Maaz Mulla, Gary L. Aistrup, Raj Kishore, J. Andrew Wasserstrom
Summary: The study demonstrated that PIP2-mediated targeting of BIN1 to the sarcolemma may be compromised during heart failure development, leading to T-tubule remodeling. Additionally, the interaction between PIP2 and cardiac BIN1 is essential for T-tubule maintenance and function, with depletion of PIP2 potentially causing T-tubule disruption.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Oncology
Tangliang Zhao, Yulin Zhou, Qingyun Wang, Xiaoming Yi, Silun Ge, Haowei He, Song Xue, Bowen Du, Jingping Ge, Jie Dong, Le Qu, Linhui Wang, Wenquan Zhou
Summary: This study collected RCC tissue and plasma samples and conducted experiments to identify QPCT, CTCF, and PIK3CA as potential targets for predicting, reversing, and treating sunitinib-resistant RCC.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Elizabeth M. Davies, Rajendra Gurung, Kai Qin Le, Katherine T. T. Roan, Richard P. Harvey, Geraldine M. Mitchell, Quenten Schwarz, Christina A. Mitchell
Summary: Dynamic positioning of endothelial tip and stalk cells is essential for angiogenesis, which is regulated by the interplay between VEGFR2 and NOTCH signaling. PI(4,5)P2 hydrolysis by INPP5K contributes to angiogenesis by regulating tip cell specification and DLL4/NOTCH signaling. The inhibition of INPP5K increases PI(4,5)P2 levels, leading to the release of II-catenin from the plasma membrane and activation of AKT signaling, which enhances DLL4/NOTCH transcription.
Article
Biochemistry & Molecular Biology
Maiwase Tembo, Rachel E. Bainbridge, Crystal Lara-Santos, Kayla M. Komondor, Grant J. Daskivich, Jacob D. Durrant, Joel C. Rosenbaum, Anne E. Carlson
Summary: This study investigates the interaction between TMEM16A channel and the membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2). The findings show that only lipids containing a phosphate at the 40 position effectively recover TMEM16A currents. These findings improve our understanding of how PI(4,5)P-2 binds to and potentiates TMEM16A channels.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yujun Hao, Baoyu He, Liping Wu, Yamu Li, Chao Wang, Ting Wang, Longci Sun, Yanhua Zhang, Yangyang Zhan, Yiqing Zhao, Sanford Markowitz, Martina Veigl, Ronald A. Conlon, Zhenghe Wang
Summary: This study reveals the oncogenic mechanism of PIK3CA helical domain mutations, showing that the release of p85 beta from mutated p110 alpha and its translocation to the nucleus enhance the activity of EZH proteins, promoting tumor growth.
NATURE COMMUNICATIONS
(2022)
Article
Veterinary Sciences
Mitsuki Uemura, Kazuhiko Ochiai, Masami Morimatsu, Masaki Michishita, Eri Onozawa, Daigo Azakami, Yumiko Uno, Yasunaga Yoshikawa, Takanori Sasaki, Masami Watanabe, Toshinori Omi
VETERINARY AND COMPARATIVE ONCOLOGY
(2020)
Article
Veterinary Sciences
Hiroyuki Tani, Sena Kurita, Ryo Miyamoto, Harumi Sawada, Aki Fujiwara-Igarashi, Masaki Michishita, Daigo Azakami, Daisuke Hasegawa, Kyoichi Tamura, Makoto Bonkobara
JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION
(2020)
Article
Pathology
Syunya Noguchi, Yoshiaki Kubo, Mami Araki, Miki Koh, Yuji Hamamoto, Kyoichi Tamura, Hirotada Otsuka, Akiko Yasuda, Daigo Azakami, Masaki Michishita, Satoshi Soeta
VETERINARY PATHOLOGY
(2020)
Article
Pathology
M. Michishita, Y. Ishizaki, M. Konnai, Y. Machida, R. Nakahira, H. Hatakeyama, H. Yoshimura, M. Yamamoto, S. Soeta, K. Ochiai, K. Misawa, N. Yugeta, D. Azakami
JOURNAL OF COMPARATIVE PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Amira Abugomaa, Mohamed Elbadawy, Megumi Yamanaka, Yuta Goto, Kimika Hayashi, Takashi Mori, Tsuyoshi Uchide, Daigo Azakami, Ryuji Fukushima, Toshinori Yoshida, Makoto Shibutani, Risako Yamashita, Mio Kobayashi, Hideyuki Yamawaki, Yuta Shinohara, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Shota Kawakami, Masaki Michishita, Motoharu Sakaue, Masami Morimatsu, Mitsuki Uemura, Nobuaki Kashiwagi, Marika Maeda, Yukino Machida, Daigo Azakami, Ai S. Egusa, Eri Onozawa, Katsumi Ishioka, Masami Watanabe, Yoshikazu Tanaka, Toshinori Omi, Kazuhiko Ochiai
Article
Oncology
Mohamed Elbadawy, Yomogi Sato, Takashi Mori, Yuta Goto, Kimika Hayashi, Megumi Yamanaka, Daigo Azakami, Tsuyoshi Uchide, Ryuji Fukushima, Toshinori Yoshida, Makoto Shibutani, Mio Kobayashi, Yuta Shinohara, Amira Abugomaa, Masahiro Kaneda, Hideyuki Yamawaki, Tatsuya Usui, Kazuaki Sasaki
Summary: The study revealed that trametinib has anti-tumor effects on bladder cancer organoids, inhibiting cell viability even at low concentrations. Trametinib also induces differentiation of organoid cells from basal to luminal, suppressing tumor growth, and shows enhanced sensitivity in combination with YAP inhibitor or carboplatin.
CANCER BIOLOGY & THERAPY
(2021)
Article
Pathology
Naoki Hanari, Tomokazu Nagashima, Yukino Machida, Yoshiaki Kubo, Yuji Hamamoto, Junichi Kamiie, Kazuhiko Ochiai, Daigo Azakami, Masaki Michishita
Summary: This case report describes a 12-year-old castrated male Jack Russell Terrier with gastric plasmacytoma. Ultrasonographic and computed tomography examinations revealed a thickened stomach wall with a solitary mass composed of neoplastic cells arranged in a diffuse pattern. Immunostaining of the neoplastic cells showed positive reactions for MUM1, CD79a and Ig lambda light chain.
JOURNAL OF COMPARATIVE PATHOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Haru Yamamoto, Mohamed Elbadawy, Koudai Fujisaka, Yomogi Sato, Takahiro Ohmori, Yuta Shinohara, Yui Hatano, Daichi Kobayashi, Ayana Gomyo, Yuji Sudo, Daigo Azakami, Tsuyoshi Uchide, Ryuji Fukushima, Shohei Morita, Amira Abugomaa, Hideyuki Yamawaki, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki
Summary: In cases of cancer metastasis, circulating tumor cells (CTCs) in blood play a key role. Efforts to study CTCs have been hindered by their rarity and difficulty in isolation. By using dogs as a model, this study demonstrated the safety and feasibility of apheresis to isolate CTCs, providing valuable insights for metastatic cancer research.
Article
Agriculture, Dairy & Animal Science
Eri Onozawa, Daigo Azakami, Seri Seki, Yuji Hamamoto, Katsumi Ishioka
Summary: A study investigated the effectiveness of a heat insulating device to prevent hypothermia in dogs and cats during magnetic resonance imaging examinations. The device significantly prevented hypothermia and heat loss from the body surface during the examinations, highlighting its potential benefits in minimizing temperature decreases during anesthesia.
Article
Pathology
Masaki Michishita, Ritsu Shibata, Yukino Machida, Manami Matsumoto, Kazuhiko Ochiai, Daigo Azakami
Summary: This study reported a case of subleukaemic T-lymphoblastic leukaemia in a 20-year-old Dutch Warmblood gelding, which is the first report of this neoplasm in horses. Clinical signs included anorexia, weight loss, fever, cough, etc.
JOURNAL OF COMPARATIVE PATHOLOGY
(2021)
Article
Medicine, Research & Experimental
Mohamed Elbadawy, Kodai Fujisaka, Haru Yamamoto, Ryouichi Tsunedomi, Hiroaki Nagano, Hiromi Ayame, Yusuke Ishihara, Takashi Mori, Daigo Azakami, Tsuyoshi Uchide, Ryuji Fukushima, Amira Abugomaa, Masahiro Kaneda, Hideyuki Yamawaki, Yuta Shinohara, Tsutomu Omatsu, Tetsuya Mizutani, Tatsuya Usui, Kazuaki Sasaki
Summary: By studying dog bladder cancer organoids, we have established organoids for normal dog bladder. This method can be used to investigate the pathogenesis of dog bladder cancer and human MIBC, and provides information on drug sensitivity and gene expression.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Veterinary Sciences
Tomohiro Oyamada, Rina Nabeta, Daigo Azakami
Summary: This clinical report describes a case of splenic hemangiosarcoma concurrent with splenic abscess. The dog developed symptoms of intra-abdominal hemorrhage several months after successful surgical management.
VETERINARY RESEARCH FORUM
(2022)
Article
Veterinary Sciences
Kento Ishikawa, Tomokazu Nagashima, Yukino Machida, Akiko Yasuda, Karin Yoshida, Michio Fujita, Kazuhiko Ochiai, Daigo Azakami, Masaki Michishita
Summary: This study presents a case of primary pulmonary diffuse large B-cell lymphoma associated with FeLV infection in a young cat. The diagnosis was confirmed through surgery, histopathology, and immunohistochemistry examinations.
JOURNAL OF FELINE MEDICINE AND SURGERY OPEN REPORTS
(2022)