Article
Plant Sciences
Juanjuan Yu, Juan M. Gonzalez, Zhiping Dong, Qianru Shan, Bowen Tan, Jin Koh, Tong Zhang, Ning Zhu, Craig Dufresne, Gregory B. Martin, Sixue Chen
Summary: Plants have developed a two-layered immune system consisting of pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). Through integrative proteomic and phosphoproteomic analyses of the tomato-Pseudomonas syringae (Pst) pathosystem, it was found that ETI response is stronger than PTI response. Protein abundance and phosphorylation changes revealed key regulators involved in signaling, oxidative stress, transport, cell remodeling, hormone signaling, and implicated potential markers for PTI and ETI.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Julien Lang, Baptiste Genot, Jean Bigeard, Jean Colcombet
Summary: This study reveals that ETI-mediating NLRs and NLR signaling components NDR1 and EDS1 can promote the salicylic acid sector of defense downstream of MPK3 activity. It also demonstrates that both sustained and transient MPK3/6 activities positively control the expression of several NLR genes. The findings highlight the intricate interplay of PTI and ETI pathways.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Dominique Arnaud, Michael J. Deeks, Nicholas Smirnoff
Summary: Stomatal defences, mediated by apoplastic ROS, are vital for plants to prevent pathogen entry. This study sheds light on the role of NADPH oxidase RBOHF in the interplay between apoplastic and cytosolic ROS dynamics during stomatal immune response. Surprisingly, the rbohF mutant showed unexpected over-oxidation of the H2O2 sensor roGFP2-Orp1, while being impaired in PAMP-triggered stomatal closure and apoplastic alkalinisation.
Article
Plant Sciences
Marta Bjornson, Priya Pimprikar, Thorsten Nuernberger, Cyril Zipfel
Summary: Plants adjust their metabolism based on environmental conditions, recognizing self and non-self molecules through pattern recognition receptors. Research suggests that plants exhibit a similar immune response to patterns of different biological origins or biochemical natures, indicating a general stress response. This initial danger response plays a crucial role in plant immunity and highlights the importance of early immune transcriptional reprogramming.
Article
Plant Sciences
Agnes Szatmari, Agnes M. Moricz, Ildiko Schwarczinger, Judit Kolozsvarine Nagy, Agnes Alberti, Miklos Pogany, Zoltan Bozso
Summary: The levels of free acetosyringone in tobacco leaves were found to increase during pattern-triggered immunity responses, exhibiting antibacterial effects. When combined with hydrogen peroxide and peroxidase, acetosyringone synergistically inhibits bacterial metabolism and proliferation rapidly in a wide range of plant pathogens, possibly through depolarization of the bacterial cell membrane.
Article
Plant Sciences
Ranran Wang, Jiaojiao Bai, Guofu Yan, Zejun Xiao, Kexin Chen, Kaikai Li, Jie Tang, Dongping Lu
Summary: This study found that enzymatic hydrolysate of fucoidan (FEH), derived from Sargassum hemiphyllum, can trigger various immune responses in plants, including ROS production, MAPK activation, gene expression reprogramming, callose deposition, stomatal closure, and plant resistance to Pseudomonas syringae pv. tomato (Pst) DC3000. Therefore, FEH has the potential to be used as a non-microbial elicitor to protect plants from pathogen infection in agricultural practices.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Keran Zhai, Di Liang, Helin Li, Fangyuan Jiao, Bingxiao Yan, Jing Liu, Ziyao Lei, Li Huang, Xiangyu Gong, Xin Wang, Jiashun Miao, Yichuan Wang, Ji-Yun Liu, Lin Zhang, Ertao Wang, Yiwen Deng, Chi-Kuang Wen, Hongwei Guo, Bin Han, Zuhua He
Summary: This study identifies the deubiquitinase PICI1 as a key player in regulating PTI and ETI in rice, promoting the production of defense metabolites by activating methionine synthetase, and ensuring coordination between PTI and ETI. NLRB receptors such as PigmR protect PICI1 from effector-mediated degradation in the plant immune system, contributing to broad-spectrum resistance.
Article
Plant Sciences
Nathan Diplock, Mael Baudin, Leslie Harden, Christopher J. Silva, Melissa L. Erickson-Beltran, Jana A. Hassan, Jennifer D. Lewis
Summary: The highly conserved angiosperm immune receptor ZAR1 recognizes diverse pathogen effector proteins by monitoring the ZRK family. The study found that ZAR1 can interact with most ZRKs, except ZRK7, in Arabidopsis thaliana. Alternative splicing of ZRK7 generates a protein that can still interact with ZAR1. The findings suggest a greater diversity of ZAR1 interactions with kinases and the potential for expanding ZAR1 immunodiversity.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Margot Raffeiner, Suayib Uestuen, Tiziana Guerra, Daniela Spinti, Maria Fitzner, Sophia Sonnewald, Susanne Baldermann, Frederik Boernke
Summary: Xanthomonas effector protein XopS enhances disease susceptibility by repressing defense gene expression and suppressing stomatal immunity through interaction with and inhibition of WRKY40, a transcriptional regulator. XopS manipulation of WRKY40 stability alters downstream gene expression and phytohormone crosstalk to promote pathogen proliferation.
Article
Biochemistry & Molecular Biology
Yingying Xing, Ning Xu, Deepak D. Bhandari, Dmitry Lapin, Xinhua Sun, Xuming Luo, Yeqiong Wang, Jidong Cao, Hongbin Wang, Gitta Coaker, Jane E. Parker, Jun Liu
Summary: The pathogen targets the plant iron sensor protein BRUTUS through the effector protein AvrRps4 to facilitate iron uptake and pathogen proliferation in Arabidopsis thaliana. AvrRps4 inhibits the degradation of iron regulatory proteins and enhances the accumulation of immune proteins, contributing to immune responses mediated by RPS4/EDS1.
Article
Plant Sciences
Jose S. Rufian, Javier Rueda-Blanco, Diego Lopez-Marquez, Alberto P. Macho, Carmen R. Beuzon, Javier Ruiz-Albert
Summary: The effector protein HopZ1a from Pseudomonas syringae suppresses plant basal immunity by targeting the Arabidopsis thaliana mitogen-activated protein kinase kinase AtMKK7 through acetylation, leading to inhibition of immune signaling pathways. In vitro and in planta assays demonstrate the interaction between HopZ1a and AtMKK7, with acetylation of a lysine residue crucial for AtMKK7 kinase activity.
Article
Biochemistry & Molecular Biology
Ayumi Matsumoto, Titus Schlueter, Katharina Melkonian, Atsushi Takeda, Hirofumi Nakagami, Akira Mine
Summary: This study developed a bioluminescence-based tool for quantitative and spatial detection of bacteria in plants. By introducing the luxCDABE luciferase operon, bacterial titers in plants can be accurately reported, and it can be applied to various plant pathogenic bacteria. Additionally, these tools can be used to study the effects of plant immunity and bacterial effectors on bacterial growth, and the spatial distribution of bacteria in plant tissues can be observed using bioluminescence imaging.
PLANT COMMUNICATIONS
(2022)
Article
Plant Sciences
Sachi Kashihara, Takafumi Nishimura, Yoshiteru Noutoshi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui
Summary: Pseudomonas amygdali pv. tabaci uses effector proteins to infect tobacco cells, but tobacco has evolved resistance genes and mechanisms. HopAZ1 is identified as a key factor that induces disease resistance in tobacco.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Clare Breit-McNally, Darrell Desveaux, David S. Guttman
Summary: The study found that the immune response of Arabidopsis thaliana is qualitatively conserved in oilseed crops, but quantitatively distinct. The level of immune conservation decreases with evolutionary distance from Arabidopsis thaliana.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Debarati Basu, Jennette M. Codjoe, Kira M. Veley, Elizabeth S. Haswell
Summary: Plants are able to sense and respond to molecular signals associated with the presence of pathogens and their virulence factors. This study investigates the potential role of a mechanosensitive ion channel, MscS-like (MSL)10, in defense against the bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. The results show that MSL10 modulates pathogen responses and suggests that mechanical signals may be utilized by plants for pathogen perception.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Review
Plant Sciences
Anurag Kashyap, Marc Planas-Marques, Montserrat Capellades, Marc Valls, Nuria S. Coll
Summary: Xylem vascular wilt pathogens cause devastating diseases in plants by disrupting water and mineral transport, but plants develop physico-chemical barriers in xylem tissue to restrict the spread of these pathogens. These barriers compartmentalize and eliminate the pathogen at the infection site, contributing to plant resistance against xylem colonizers.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Alejandro Alonso-Diaz, Santosh B. Satbhai, Roger de Pedro-Jove, Hannah M. Berry, Christian Goschl, Cristiana T. Argueso, Ondrej Novak, Wolfgang Busch, Marc Valls, Nuria S. Coll
Summary: This study investigated the genetic determinants of root immunity against the soil-borne pathogen Ralstonia solanacearum in Arabidopsis, highlighting the role of cytokinin signaling in early defense responses. The researchers used a high-throughput in vitro infection system to identify allelic variation in genes related to cytokinin metabolism, providing insights into the mechanisms underlying root defenses against the devastating bacterial wilt disease.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Hayoung Moon, Ankita Pandey, Hayeon Yoon, Sera Choi, Hyelim Jeon, Maxim Prokchorchik, Gayoung Jung, Kamil Witek, Marc Valls, Honour C. McCann, Min-Sung Kim, Jonathan D. G. Jones, Cecile Segonzac, Kee Hoon Sohn
Summary: This study identified a T3E protein RipAZ1 in Ralstonia solanacearum as an avirulence determinant in Solanum americanum, inducing cell death response and leading to acquired virulence when lost. The naturally occurring C-terminal truncation of RipAZ1 results in loss of cell death induction, while the central region of RipAZ1 is sufficient to induce cell death. RipAZ1 may activate defense in host cell cytoplasm, conferring avirulence in S. americanum. This work opens the way for the identification of disease resistance genes specific to RipAZ1 recognition against bacterial wilt disease.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
R. de Pedro-Jove, M. Puigvert, P. Sebastia, A. P. Macho, J. S. Monteiro, N. S. Coll, J. C. Setubal, M. Valls
Summary: This study is the first to describe the dynamic transcriptional changes of Ralstonia solanacearum within the plant during infection, defining four main genetic programs that determine pathogen physiology during plant colonization and providing key information on bacterial states in different infection stages, shedding light on the potato infection process by R. solanacearum.
Article
Plant Sciences
Yang Niu, Shouyang Fu, Gong Chen, Huijuan Wang, Yisa Wang, JinXue Hu, Xin Jin, Mancang Zhang, Mingxia Lu, Yizhe He, Dongdong Wang, Yue Chen, Yong Zhang, Nuria S. Coll, Marc Valls, Cuizhu Zhao, Qin Chen, Haibin Lu
Summary: This study demonstrated that the RipAW effector triggers effector-triggered immunity (ETI) in two evolutionally related species, Nicotiana benthamiana and Nicotiana tabacum. The research showed that there are commonalities and specificities in RipAW-activated ETI between these two species, suggesting Nicotiana species have acquired different abilities to perceive RipAW and activate plant defenses during plant-pathogen co-evolution.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Anurag Kashyap, Alvaro Luis Jimenez-Jimenez, Weiqi Zhang, Montserrat Capellades, Sumithra Srinivasan, Anna Laromaine, Olga Serra, Merce Figueras, Jorge Rencoret, Ana Gutierrez, Marc Valls, Nuria S. Coll
Summary: Tomato varieties resistant to the bacterial wilt pathogen can restrict bacterial movement by forming vascular coatings composed of ligno-suberin and tyramine-derived hydroxycinnamic acid amides. Overexpressing genes of the ligno-suberin pathway in susceptible tomato varieties enhances resistance to the pathogen.
Article
Plant Sciences
Liang Yang, Zhouling Wei, Marc Valls, Wei Ding
Summary: Metabolic analyses were used to identify metabolite differences in tobacco xylem sap in response to Ralstonia pseudosolanacearum infection, revealing metabolites that can promote bacterial growth.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Jierui Zhao, Mai Thu Bui, Juncai Ma, Fabian Kuenzl, Lorenzo Picchianti, Juan Carlos De la Concepcion, Yixuan Chen, Sofia Petsangouraki, Azadeh Mohseni, Marta Garcia-Leon, Marta Salas Gomez, Caterina Giannini, Dubois Gwennogan, Roksolana Kobylinska, Marion Clavel, Swen Schellmann, Yvon Jaillais, Jiri Friml, Byung-Ho Kang, Yasin Dagdas
Summary: The research reveals the presence of amphisome compartments in plant cells, which play a crucial role in the maturation and fusion of autophagosomes with vacuoles.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Plant Sciences
Nuria Real, Irene Villar, Irene Serrano, Celia Guiu-Aragones, Ana Montserrat Martin-Hernandez
Summary: Resistance to cucumber mosaic virus in melon is controlled by a recessive resistance gene encoding a vacuolar protein sorting 41, which restricts the virus to bundle sheath cells. The resistant accessions show specific intravacuolar structures that colocalize with late endosomes.
Article
Plant Sciences
Anurag Kashyap, Alvaro Jimenez-Jimenez, Merce Figueras, Olga Serra, Marc Valls, Nuria S. Coll
Summary: Suberin, a wall polymer, plays various roles in plant development and stress responses. It is deposited between the plasma membrane and the primary cell wall in specialized tissues. The deposition of suberin is preceded by cell wall lignification, and the role of ferulates in coupling both polymers is not fully understood. A study using the promoter of tomato suberin feruloyl transferase (FHT/ASFT) and GUS fusion demonstrated that ferulate deposition coincides with FHT promoter activation, providing a means to identify early suberizing cells rich in ferulic acid. This finding can be used as a marker for early suberization events and potentially aid in varietal improvement programs.
Article
Plant Sciences
Gloria Escola, Victor M. Gonzalez-Miguel, Sonia Campo, Mar Catala-Forner, Concha Domingo, Luis Marques, Blanca San Segundo
Summary: In this study, a blast-resistant rice breeding line, COPSEMAR9, was obtained through hybridization and a high-quality genome sequence was obtained. The presence of rice blast resistance genes and defense-related genes, as well as miRNAs potentially contributing to blast resistance, were identified in COPSEMAR9.