☆ 4.4 Article

Silencing a Myzus persicae Macrophage Inhibitory Factor by Plant-Mediated RNAi Induces Enhanced Aphid Mortality Coupled with Boosted RNAi Efficacy in Transgenic Potato Lines

MOLECULAR BIOTECHNOLOGY (2022)

Journal

MOLECULAR BIOTECHNOLOGY

Volume 64, Issue 10, Pages 1152-1163

Publisher

SPRINGERNATURE

DOI: 10.1007/s12033-022-00498-w

Keywords

Plant-mediated RNAi; Potato aphid; Macrophage inhibitory factor 1; Myzus persicae bioassay

Funding

Higher Education Commission (HEC)

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Abstract

Using RNA interference technique and transgenic potato lines, successful gene silencing and aphid mortality of Myzus persicae were induced, demonstrating the great potential of plant-mediated RNA interference in protecting crops from aphid pests.

Myzus persicae causes considerable losses to crops as a major pest. The damage is direct by feeding and also partly indirect because it vectors plant viruses. The currently available control strategies rely on unsafe and nonecofriendly chemical pesticide applications. Plant-mediated RNA interference (RNAi) has emerged as a powerful tool in crop protection from insect pests. Aphid salivary proteins are essential for phloem feeding and act as mediators of the complex interactions between aphids and their host plants. We documented the efficacy of dsRNA directed against macrophage inhibitory factor (MIF1) of M. persicae to induce aphid mortality and gene silencing through the generation of transgenic potato lines. A binary construct harbouring dsMIF1 driven by the CaMV35S promoter was introduced into the local potato variety 'AGB-white' by Agrobacterium-mediated transformation. PCR and Southern blotting validated the transgene presence and genomic integration in seven transgenic potato lines. An in vitro detached leaf assay revealed a significantly high aphid mortality of 65% in the transgenic potato line sDW-2, while the aphid mortality was 77% in the sDW-2 transgenic line during the in planta bioassay in comparison with 19% aphid mortality in the control nontransgenic potato line. A significantly high silencing effect was observed in the mRNA expression of MIF1, which was reduced to 21% in aphids fed on the transgenic potato line sDW-2. However, variable knockdown effects were found among six other transgenic potato lines, ranging from 30 to 62%. The study concluded that plant-mediated silencing of aphid RNA induces significant RNAi in M. persicae, along with enhanced aphid mortality.

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Ladybird-Mediated Indirect Interactions between Two Aphid Species When Using a Banker Plant System

Yajie Yang, Jie Wang, Yingying Mi, Junjie Gu, Giovanni Benelli, Nicolas Desneux, Su Wang, Shu Li, Yanli Yue

Summary: Banker plant systems can control pests sustainably by introducing natural enemies and providing alternative prey. This study investigated the dynamic relationship among the elements of a banker plant system and its impact on aphid pest control. The research system used in the study was the Coccinella septempunctata-Megoura japonica-Vicia faba banker plant system for controlling Myzus persicae on Capsicum annum. The study tested the effects of different release time of predators, different initial numbers of alternative prey, and different initial ratios of target pests/alternative prey on the indirect interaction of two aphids and the biological control effect of shared predators.

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