Integrated analysis of fish intestine biomarkers: Complementary tools for pollution assessment

The gastrointestinal tract and its enteric nervous system are the first routes of food and xenobiotics uptake. Considering the importance of this organ, this study evaluated intestinal biomarkers of Sphoeroides testudineus integrating the data to generate tools for pollution assessment. The fish were collected in three sites of Sao Paulo Coast and their intestines were analyzed for biochemical, histology, and neuronal density and morphometry biomarkers. To evaluate the differences among the data, a PERMANOVA was applied, followed by a FA/PCA. The PERMANOVA indicated differences (P < 0.001) between the regions (RA, A1, and A2). Four factors were extracted from the FA/PCA (62% cumulative), showing that the animals from A2 presented severe alterations, mainly in intestinal morphometry and neuronal density. A1 alterations refer mainly to the increase of neuronal metabolism. Our results also evidence a gradient of environmental quality related to the protection level (AR > A1 > A2).

Automated summary

This study evaluated intestinal biomarkers of Sphoeroides testudineus to develop tools for pollution assessment. The analysis of biochemical, histology, neuronal density, and morphometry data revealed regional differences, with A2 showing severe alterations and A1 mainly characterized by increased neuronal metabolism. The results also demonstrated a gradient of environmental quality related to the protection level.