Article
Chemistry, Analytical
Maroua Hamami, Abdelmoneim Mars, Noureddine Raouafi
Summary: A new nanometric aptasensing platform was developed for detecting aflatoxin M1 in milk, showing good sensitivity, low LOD, and good metrological performances. The platform was applied to detect AFM1 in pasteurized cow milk with promising results.
MICROCHEMICAL JOURNAL
(2021)
Article
Spectroscopy
Seyed Hamid Jalalian, Parirokh Lavaee, Mohammad Ramezani, Noor Mohammad Danesh, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Aflatoxins pose significant risks to human health and the economy. Developing a rapid, precise, and inexpensive detection method is crucial. The colorimetric aptasensor based on gold nanoparticles can detect AFM(1) with high sensitivity and has practical applications in milk samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Hongyan Zhang, Weiwei Mao, Yijin Hu, Xiaohong Wei, Lishan Huang, Shen Fan, Mingqing Huang, Yu Song, Yuyan Yu, FengFu Fu
Summary: A highly sensitive and specific visual detection method for aflatoxin B1 (AFB1) has been developed based on the combination of aptamer, rolling circle amplification (RCA), and enzyme catalysis. The method can achieve a wide linear detection range and low detection limit, and exhibits excellent specificity in complex sample matrices, showing great potential for practical AFB1 detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Mariam Saad, F. Rafael Castiello, Sebastien P. Faucher, Maryam Tabrizian
Summary: This study introduces a novel SPRi-based titration assay using L. pneumophila aptamers for Legionella detection. High salt buffer and 500 nM of immobilized cApt resulted in optimal signal response, with only SSC buffer facilitating incremental binding of aptamers to L-p. The assay showed the potential for SPRi-based L-p detection with specific and negligible detection of other water system microbes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Kaixuan Wang, Nuanfei Zhu, Yumo Li, Hu Zhang, Beibei Wu, Jian Cui, Jun Tang, Zhugen Yang, Fang Zhu, Zhen Zhang
Summary: In this study, a universal detection system based on a novel polyadenine-based tetrahedral DNA nanostructure was developed, which can rapidly and sensitively detect various targets in different fields.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Ying Lan, Baixun He, Cherie S. Tan, Dong Ming
Summary: Aptamers are functional recognition ligands with high specificity and affinity, which can be utilized in biosensors for sensing biomolecules. Aptasensors, based on aptamers, are specific, sensitive, and cost-effective, and have shown advantages when combined with smartphone devices for point-of-care testing.
Article
Biophysics
Neil Adrian P. Ondevilla, Tak-Wah Wong, Nan-Yao Lee, Hsien-Chang Chang
Summary: Traditional immunosensors are often limited by low sensitivity and long detection times, but a rapid electrochemical aptasensor integrated with an AC electroosmotic (ACEO) flow phenomenon has been developed, showing enhanced target hybridization of miR-155. By optimizing electrokinetic conditions, the sensor achieved highly sensitive detection of miR-155 within a short time frame, enabling rapid and real-time sensing of target biomarkers for early infection detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Food Science & Technology
Hai Chi, Guoqin Liu
Summary: A fluorometric sandwich biosensor combining molecularly imprinted polymer (MIP) with an aptamer was developed for the detection of Aflatoxin B1 (AFB1). The combination of porous carbon (PC) and MIP layer provided abundant sites for efficient recognition of AFB1. The aptamer-modified CdTe/ZnS achieved efficient sandwich analysis of AFB1. The sandwich biosensor showed excellent selectivity and high sensitivity, with a dynamic linear range of 0.01-20 ng/mL and a low detection limit of 4.0 pg/mL in spiked edible oil samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Saipriya Ramalingam, Christopher M. Collier, Ashutosh Singh
Summary: Antibiotics are widely used in veterinary science to promote animal health and feed efficiency, but concerns arise due to the potential residues in milk. Therefore, quick and reliable testing methods are essential to ensure the safety of the produced milk.
Article
Agriculture, Dairy & Animal Science
Yuanyuan Hui, Haishuai Peng, Fuxin Zhang, Lei Zhang, Liu Yufang, Aiqing Zhao, Rong Jia, Bini Wang, Yuxuan Song
Summary: This study developed a novel electrochemical aptasensor for sensitive and specific detection of AFM(1) in dairy products. The method showed a wide detection range and low detection limit, and it performed well in the detection of AFM(1) in cow, goat, and sheep milk samples.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biophysics
Xiaoqin Liu, Ting Wang, Yuwei Wu, Yifu Tan, Ting Jiang, Ke Li, Beibei Lou, Liwei Chen, Yanfei Liu, Zhenbao Liu
Summary: This article summarizes fluorescence detection methods based on aptamers for intracellular molecular detection. By combining different nanostructures, these biosensors can enter living cells for analysis and enable in situ monitoring. The principles, limit of detection, advantages, and disadvantages of different platforms for intracellular molecular fluorescence response are reviewed, and the current challenges and future developments are discussed.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Rui Li, Linzhi Li, Tianzeng Huang, Xing Liu, Qi Chen, Guiying Jin, Hongmei Cao
Summary: A novel colorimetric aptasensor was developed using unmodified gold nanoparticles for rapid and sensitive detection of multiple mycotoxins. The sensor showed high specificity and low limits of detection for OTA and AFB(1), making it suitable for on-site analysis in rice samples.
ANALYTICAL METHODS
(2021)
Article
Engineering, Environmental
Asma Verdian, Zeinab Rouhbakhsh, Ebrahim Fooladi
Summary: The novel liquid crystal aptasensor platform utilizes a triple-helix molecular conformational switch for PCB77 detection with a limit of quantification of 1.5 x 10 (-5) mu g/L and comparable selectivity. This system can detect PCB77 in tap water, environmental water, and milk, and has the potential for label-free and portable detection of different targets.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Lijun Zhao, Jianfei Mao, Li Hu, Shu Zhang, Xiaofeng Yang
Summary: A rapid signal amplified AFB1 detection system based on SRCHA has been developed, which utilizes a catalyzed hairpin assembly reaction to significantly enhance the fluorescence signal for AFB1 detection within 15 minutes with a low detection limit. This system shows great potential for on-site rapid detection of AFB1.
ANALYTICAL METHODS
(2021)
Review
Chemistry, Analytical
Donato Calabria, Martina Zangheri, Seyedeh Rojin Shariati Pour, Ilaria Trozzi, Andrea Pace, Elisa Lazzarini, Maria Maddalena Calabretta, Mara Mirasoli, Massimo Guardigli
Summary: The presence of hidden allergens in food products has led to the urgent need for rapid and reliable analytical methods. Portable biosensors based on immunoassays and aptamers have shown promise as alternative detection methods for food allergens.
