Article
Multidisciplinary Sciences
Dina Abdelaziz, Amr Hefnawy, Essam Al-Wakeel, Abeer El-Fallal, Ibrahim M. El-Sherbiny
Summary: A new series of nanofibrous scaffolds were prepared in this study for GTR/GBR applications, with enhanced cell viability and antibacterial activity through the incorporation of hydroxyapatite nanoparticles and green-synthesized silver nanoparticles, respectively. The scaffolds showed a desirable degradation profile, losing around 40-70% of their mass in 8 weeks, making them promising for both GTR and GBR applications.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Jing Xiang, Yuzhou Li, Mingxing Ren, Ping He, Fengyi Liu, Zheng Jing, Yong Li, He Zhang, Ping Jia, Sheng Yang
Summary: The novel nanocomposite multifunctionalized sandwich-like GBR membrane (SGM) shows enhanced osteogenic properties and antimicrobial activities, holding promise for guided bone regeneration applications.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Physical
Helena Zelikman, Gil Slutzkey, Ofir Rosner, Shifra Levartovsky, Shlomo Matalon, Ilan Beitlitum
Summary: This in vitro study evaluated the antibacterial effect of three commercial non-resorbable polytetrafluoroethylene membranes and found that two of them significantly reduced the growth of a specific bacterial species.
Review
Biochemistry & Molecular Biology
Irina-Georgeta Sufaru, Georgiana Macovei, Simona Stoleriu, Maria-Alexandra Martu, Ionut Luchian, Diana-Cristala Kappenberg-Nitescu, Sorina Mihaela Solomon
Summary: Periodontal guided bone regeneration (GBR) and guided tissue regeneration (GTR) involve the use of a membrane to create a protected area for tissue regeneration. Recent advancements in 3D printing technology have led to innovations in periodontal regeneration methods, including bioprinting and tissue engineering.
Article
Dentistry, Oral Surgery & Medicine
Balazs Feher, Karol Ali Apaza Alccayhuaman, Franz Josef Strauss, Jung-Seok Lee, Stefan Tangl, Ulrike Kuchler, Reinhard Gruber
Summary: The alignment of bilayer collagen membranes does not affect bone regeneration, as placing the membranes with the dense layer towards the bone defect or towards the soft tissues were both effective in promoting bone regeneration. The study supports the osteoconductive properties of collagen membranes regardless of their alignment.
INTERNATIONAL JOURNAL OF IMPLANT DENTISTRY
(2021)
Article
Chemistry, Medicinal
Stefanos Kikionis, Konstantina Iliou, Aikaterini G. Karra, Georgios Polychronis, Ioannis Choinopoulos, Hermis Iatrou, George Eliades, Efthymia Kitraki, Ioulia Tseti, Spiros Zinelis, Efstathia Ioannou, Vassilios Roussis
Summary: Nanofibrous GTR/GBR membranes based on carrageenans and other biocompatible polymers were developed for the regeneration of periodontal tissue. The membranes exhibited favorable morphological, thermal, and mechanical properties, and demonstrated the potential for periodontal tissue regeneration through evaluation of cell attachment, biocompatibility, and osteogenic differentiation.
Article
Biochemical Research Methods
Jiayang Li, Dongming He, Longwei Hu, Siyi Li, Chenping Zhang, Xuelai Yin, Zhen Zhang
Summary: Decellularized periosteum has been found to have immunomodulatory properties, facilitating bone regeneration by modulating macrophage polarization and promoting migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. In vivo experiments on a rat cranial critical-size defect model confirmed the beneficial effects of decellularized periosteum on the local immune microenvironment and bone regeneration.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Multidisciplinary
Behnaz Malekahmadi, Vahid Esfahanian, Fatemeh Ejeian, Maziar Ebrahimi Dastgurdi, Maria Agheb, Faranak Kaveian, Mohammad Rafienia, Mohammad Hossein Nasr-Esfahani
Summary: Developing new barrier membranes with improved biomechanical characteristics has gained attention in the field of periodontal tissue regeneration. In this study, the electrospun polycaprolactone/gelatin membranes were enriched with bioglass or Cu-doped bioglass, and the cellular adhesion and proliferation potential were examined. The results showed that all modified membranes had reasonable attachment and proliferation levels of alveolar bone marrow-derived mesenchymal stem cells. The addition of copper provided superiority for periodontal tissue regeneration in one of the modified membranes.
Article
Biochemistry & Molecular Biology
Ilya Likhachev, Nikolay K. Balabaev, Oxana Galzitskaya
Summary: This study investigates the mechanism of AMP peptide penetration through the membrane bilayer, finding a strong correlation between the instability index and membrane reaction force. It is interesting to note that peptides with different compositions exhibit varying resistance to the bilayer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Sedef Tamburaci, Funda Tihminlioglu
Summary: The study aims to fabricate a novel bilayer nanocomposite membrane for enhanced guided bone regeneration, with Si-nHap incorporation improving the biocompatibility and supporting osteogenic cell bioactivity at the defect site.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Geng Liu, Jianbo Li, Xue Shi
Summary: The use of nanofiber membranes with antibacterial properties and the incorporation of doxycycline (DOX) can promote tissue regeneration and inhibit bacterial growth. The synthesized DOX-loaded nanofibers have a smooth surface and uniform diameter distribution. The release of DOX from the membranes shows sustained release and the inhibitory effect on bacterial growth increases with higher drug loading. This study suggests the potential application of these nanofiber membranes in the treatment of peri-implantitis.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Xuanhao Wu, Sisi Cao, Deoukchen Ghim, Qisheng Jiang, Srikanth Singamaneni, Young-Shin Jun
Summary: The newly developed solar-driven photothermal membrane distillation (PMD) system offers efficient vapor generation, condensation, and collection, utilizing a bilayer structure to enhance optical/photothermal activities and increase membrane porosity. This system shows high permeate flux and solar energy-to-collected water efficiency, providing a promising solution for decentralized desalination in remote areas.
Article
Dentistry, Oral Surgery & Medicine
Anna Maria Tanneberger, Sarah Al-Maawi, Carlos Herrera-Vizcaino, Anna Orlowska, Alica Kubesch, Robert Sader, C. J. Kirkpatrick, Shahram Ghanaati
Summary: The study characterized the cellular reaction to a xenogeneic resorbable collagen membrane of porcine origin in Wistar rats over 30 days. It was found that the membrane induced a large number of mononuclear cells, including lymphocytes, macrophages, and fibroblasts, as well as multinucleated giant cells (MNGCs) expressing specific markers. The significant increase in MNGC number correlated with a high rate of vascularization compared to the control group, suggesting a potential role for MNGCs in biomaterial degradation.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Chemistry, Physical
Yong Ju Yun, Ok Ja Yoon, Dong Ick Son, Yongseok Jun
Summary: A novel metal-coated bacteria cellulose nanofiber bilayer membrane (MBCBM) was developed for high-performance hydrovoltaic power-generation devices, with a Schottky barrier incorporated to enhance electric power output.
Article
Engineering, Biomedical
Ximena Vidal-Gutierrez, Gina Prado-Prone, Sandra E. Rodil, Cristina Velasquillo, Ibarra Clemente, Phaedra Silva-Bermudez, Argelia Almaguer-Flores
Summary: Periodontitis is a chronic inflammatory disease characterized by the destruction of periodontal tissues, and Guided tissue regeneration (GTR) is an effective clinical procedure for periodontal therapy. The integration of non-antibiotic, antimicrobial materials such as Bismuth subsalicylate (BSS) into membranes has emerged as a novel strategy to combat bacterial infections. The study successfully developed PCL-G-BSS membranes with antibacterial properties for potential use in periodontal GTR treatments.
BIOMEDICAL MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Pasiree Thongthai, Haruaki Kitagawa, Susita Noree, Yasuhiko Iwasaki, Yuhan Liu, Gabriela Laranjeira Abe, Satoshi Yamaguchi, Satoshi Imazato
Summary: The copolymer combining protein-repellent and antibacterial agents showed long-lasting effects in terms of protein repulsion, antibacterial activity, and anti-biofilm formation.
DENTAL MATERIALS JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
A. Li, J. Sasaki, T. Inubushi, G. L. Abe, J. E. Nor, T. Yamashiro, S. Imazato
Summary: This study reveals the crucial role of heparan sulfate (HS) in endothelial differentiation and vasculogenesis of dental pulp stem cells (DPSCs). The results show that inhibiting HS activity decreases the proliferation and sprouting ability of DPSCs and reduces the expression of proangiogenic markers. Silencing the HS biosynthesis key enzyme EXT1 alters the gene expression profile and vascular formation capacity of DPSCs. The findings provide new perspectives for the application of HS in tissue engineering and dental pulp regeneration.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Fan Deng, Hirohiko Sakai, Haruaki Kitagawa, Tomoki Kohno, Pasiree Thongthai, Yuhan Liu, Ranna Kitagawa, Gabriela L. Abe, Jun-ichi Sasaki, Satoshi Imazato
Summary: This study fabricated pH-responsive Zn2+-releasing glass particles and evaluated their solubilities, ion-releasing characteristics, and antibacterial properties. The solubility and concentration of Zn2+ released in the medium increased with a decrease in medium pH. Glass particles with higher zinc content demonstrated higher concentrations of released Zn2+ and exhibited good antibacterial effects even under acidic conditions, which may contribute to the prevention of diseases with lower acid resistance.
Article
Biotechnology & Applied Microbiology
Natnicha Jiravejchakul, Gabriela L. Abe, Martin Loza, Soyoung Park, Ponpan Matangkasombut, Jun-Ichi Sasaki, Satoshi Imazato, Diego Diez, Daron M. Standley
Summary: This study provides insights into the cellular and molecular composition of dental pulp and the crosstalk between different cell types. Fibroblasts in dental pulp have a unique gene expression profile and play a crucial role in maintaining tissue homeostasis through their interaction with other resident cells. Fibroblast-derived growth factors may have potential therapeutic applications in dental treatments.
Article
Cell & Tissue Engineering
Aonan Li, Jun-Ichi Sasaki, Gabriela L. Abe, Chihiro Katata, Hirohiko Sakai, Satoshi Imazato
Summary: By incorporating dental pulp stem cells (DPSCs) as a vascular source into bone marrow-derived mesenchymal stem cell (BMSC) constructs, vascularized scaffold-free bone organoids were successfully fabricated. This study demonstrates the promising potential of this biomimetic biomaterial for bone regenerative medicine and drug development.
STEM CELLS INTERNATIONAL
(2023)
Review
Engineering, Biomedical
Satoshi Imazato, Toshiyuki Nakatsuka, Haruaki Kitagawa, Jun-Ichi Sasaki, Satoshi Yamaguchi, Shuichi Ito, Hiroki Takeuchi, Ryota Nomura, Kazuhiko Nakano
Summary: Surface Pre-Reacted Glass-ionomer (S-PRG) filler can release multiple ions and exhibit various bioactivities, making it beneficial for various dental treatments and care, such as restorative treatment, caries prevention/management, vital pulp therapy, endodontic treatment, and prevention/treatment of periodontal disease.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Masaki Kinoshita, Satoru Yamada, Junichi Sasaki, Shigeki Suzuki, Tetsuhiro Kajikawa, Tomoaki Iwayama, Chiharu Fujihara, Satoshi Imazato, Shinya Murakami
Summary: PLAP-1 plays a crucial role in the periodontal ligament by inhibiting inflammatory responses and maintaining periodontal tissue homeostasis. Knockout of PLAP-1 leads to changes in periodontal ligament structures and accelerated bone loss in periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
P. Nag, T. Inubushi, J. I. Sasaki, T. Murotani, S. Kusano, Y. Nakanishi, Y. Shiraishi, H. Kurosaka, S. Imazato, Y. Yamaguchi, T. Yamashiro
Summary: Teeth consist of enamel, dentin, and cementum, and tooth malformation can be caused by genetic and environmental factors. The cell surface hyaluronidase TMEM2 plays a critical role in enamel formation and mineralization. TMEM2 deficiency leads to reduced enamel thickness, enamel deficiency, abnormal enamel structure, and impaired ameloblast differentiation and mineralization. TMEM2 is also involved in cell adhesion, tight junction gate function, and actin filament assembly in ameloblasts.
JOURNAL OF DENTAL RESEARCH
(2023)