Denitrification performance of nitrate-dependent ferrous (Fe2+) oxidizing Aquabacterium sp. XL4: Adsorption mechanisms of bio-precipitation of phenol and estradiol

In this study, a nitrate-dependent ferrous (Fe2+) oxidizing strain under anaerobic conditions was selected and identified as XL4, which belongs to Aquabacterium. The Box-Behnken design (BBD) was used to optimize the growth conditions of strain XL4, and the nitrate removal efficiency of strain XL4 (with 10% inoculation dosage, v/v) could reach 91.41% under the conditions of 30.34 degrees C, pH of 6.91, and Fe2+ concentration of 19.69 mg L-1. The results of Fluorescence excitation-emission matrix spectra (EEM) revealed that the intensity of soluble microbial products (SMP), aromatic proteins and the fulvic-like materials were obvious difference under different Fe2+ concentration, pH, and temperature. X-ray diffraction (XRD) data confirmed that the main components of bio-precipitation were Fe3O4 and FeO(OH), which were believed to be responsible for the adsorption of phenol and estradiol. Furthermore, the maximum adsorption capacity of bio-precipitation for phenol and estradiol under the optimal conditions were 192.6 and 65.4 mg g(-1), respectively. On the other hand, the adsorption process of phenol and estradiol by bio-precipitation confirmed to the pseudo-second-order and Langmuir model, which shows that the adsorption process is chemical adsorption and occurs on the uniform surface.

Automated summary

In this study, a nitrate-dependent ferrous oxidizing strain XL4 belonging to Aquabacterium was selected and optimized using the Box-Behnken design. The results showed that strain XL4 had a nitrate removal efficiency of 91.41% under specific conditions. Fluorescence excitation-emission matrix spectra and X-ray diffraction analysis revealed that the bio-precipitation produced by strain XL4 mainly consisted of Fe3O4 and FeO(OH), and had a high adsorption capacity for phenol and estradiol.