Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms23073661
Keywords
phagocytosis; phagosomes; RPE; methods; mice
Funding
- National Institutes of Health [EY026215]
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This review compares studies on the rhythm and quantity of RPE phagocytosis, using different in vivo model systems and assessment methods. The impact of measurement methodology on the observation and analysis of RPE phagocytosis is discussed. Studies on mice suggest that genetic background and housing conditions may affect the results. Overall, comparing RPE phagocytosis studies using differing methodology and strains of the same species is not as straightforward as previously thought.
In the vertebrate retina, the light-sensitive photoreceptor rods and cones constantly undergo renewal by generating new portions of the outer segment and shedding their distal, spent tips. The neighboring RPE provides the critical function of engulfing the spent material by phagocytosis. RPE phagocytosis of shed rod outer segment fragments is a circadian process that occurs in a burst of activity shortly after daily light onset with low activity at other times, a rhythm that has been reported for many species and over 50 years. In this review, we compare studies on the rhythm and quantity of RPE phagocytosis using different in vivo model systems and assessment methods. We discuss how measurement methodology impacts the observation and analysis of RPE phagocytosis. Published studies on RPE phagocytosis investigating mice further suggest that differences in genetic background and housing conditions may affect results. Altogether, a comparison between RPE phagocytosis studies performed using differing methodology and strains of the same species is not as straightforward as previously thought.
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