Anticataractogenesis Mechanisms of Curcumin and a Comparison of Its Degradation Products: An in Vitro Study

Curcumin (Cur) exhibits anticataractogenesis activity. This study aimed to compare the activities of Cur with those of its degradation products in a series of in vitro lens protein turbidity assays. The results show that Cur (200 mu M) ameliorates selenite-induced crystallin aggregation, and the mean OD value was 0.10 +/- 0.02 (p < 0.05), which was significantly different from controls (0.15 +/- 0.01) after incubating for 3 days. However, Cur did not significantly inhibit calcium-induced proteolysis after incubating for 3 days. Such results were supported by isothermal titration calorimetry observation that Cur binds with selenite but not with calcium. Presence of Cur and the degradation products examined (ferulic acid, cinnamic acid, vanillin, and vanillic acid) indicates significantly protective activities on lens gamma-crystallins after UVC exposure for 3 h. Among the compounds examined, only ferulic acid exhibited a significant inhibitory effect against UVB-induced turbidity with a mean OD of 0.32 +/- 0.01 (p < 0.05), which was significantly different from controls (0.49 +/- 0.02). The previously reported anticataract effects of Cur may stem not only from Cur but also from its degradation products through various cataractogenesis mechanisms in vitro.