Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 64, Issue 4, Pages 831-839Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05429
Keywords
dipeptidyl peptidase IV; silver carp protein; peptide inhibitor; in silico analysis; Pro-motif peptide; LC-MS/MS identification
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The dipeptidyl peptidase N (DPP-IV)inhibitory bioactivity of silver carp protein (SCP) hydrolysates were investigated, and their containing efficacious DPP-IV-inhibitory peptides were explored by in silico hydrolysis analysis, peptide separation combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, and chemical synthesis. SCP hydrolysates generated by six proteases all showed efficient DPP-IV-inhibitory activities, and Neutrase-generated hydrolysates had the greatest DPP-IV inhibition (IC50 of 1.12 mg/mL). In silico Neutrase hydrolysis revealed hundreds of fragments released from myosin, actin, and collagen of SCPs, which include different Pro-motif peptides but only three reported peptidic DPP-IV inhibitors with moderate or weak bioactivity, In addition, three new DPP-IV-inhibitory peptides were identified using LC-MS/MS; in particular, LPHDI and APGPAGP showed high DPP-IV-inhibitory activity with IC50 of 105.44 and 229.14 mu M, respectively, and behaved in competitive/non-competitive mixed-type DPP-IV inhibition mode. The results indicate that the SCP-derived DPP-IV-inhibitory peptides could be potential functional ingredients in the diabetic diet.
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