Article
Endocrinology & Metabolism
Camille E. Powe, Joseph J. Locascio, Larraine Huston Gordesky, Jose C. Florez, Patrick M. Catalano
Summary: The study found that Stumvoll and AUC(ins)/AUC(glu) are valid OGTT-based measures of insulin secretory response during pregnancy, and can effectively reflect the changes in insulin secretion.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Nanoscience & Nanotechnology
Xia Huang, Shangcong Han, Zuxian Chen, Lei Zhao, Changduo Wang, Qingyang Guo, Yanfeng Li, Yong Sun
Summary: LDH nanoparticles modified by DCA and HA improved the absorption efficiency of insulin by opening the TJs of cells and interacting with the cholic acid transporter receptor protein.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Huda S. AlSalem, Nisreen M. Abdulsalam, Najla A. Khateeb, Mona S. Binkadem, Nahlah A. Alhadhrami, Abdalla M. Khedr, Rehab Abdelmonem, Kamel R. Shoueir, Eman Hassan Nadwa
Summary: Chitosan was modified and functionalized to improve oral insulin delivery. The Cs-DNS NPs had a particle size of 33.29 ± 5.08 nm and positive zeta potential. In vitro experiments showed high insulin entrapment efficiency and gradual release at different pH levels. In vivo experiments demonstrated that insulin-incorporated Cs-DNS NPs effectively lowered serum glucose levels, with a bioavailability of 17.5 ± 0.31% and pharmacological availability of 8.6 ± 0.8%.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Weizhi Wang, Chenggong Yu, Fangfang Zhang, Yuxuan Li, Bo Zhang, Jie Huang, Zhijun Zhang, Liang Jin
Summary: A drug delivery system, GC-CA@PLGA NPs, has been developed to improve the oral administration of insulin with excellent stability, good biocompatibility, and significantly increased bioavailability for potential application in the treatment of diabetes.
BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Xiomara Calderon-Colon, Yichuan Zhang, Olivia Tiburzi, Jialu Wang, Shenda Hou, Giorgio Raimondi, Julia Patrone
Summary: A nanostructured lipid carrier (NLC) containing insulin peptide was designed and formulated for oral delivery using the design of experiments (DOE) statistical method. The study found that the particles had good biocompatibility, were readily taken up by immune cells in vitro, and accumulated in regional lymph nodes after oral administration. This platform technology has potential for the development of oral delivery-based antigen specific immunotherapies for autoimmune diseases.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Pharmacology & Pharmacy
Mona M. Elkhatib, Amir Ali, Ali S. Al-Badrawy
Summary: An advanced gut iontophoresis technology has been developed for delivering insulin nanoparticles across the gut wall, showing promising results in enhancing insulin transport and inducing intense hypoglycemic effects. Cell viability assays confirmed the non-toxic effects of nanoparticles on intestinal cells, suggesting that nanoparticle gut iontophoresis could be a potential non-invasive technique for oral insulin delivery.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Pharmacology & Pharmacy
Li Shangyong, Zhang Hui, Chen Kaiwei, Jin Mengfei, Vu Son Hai, Samil Jung, He Ningning, Zheng Zhou, Myeong-Sok Lee
Summary: Oral drug delivery systems have advantages of simplicity and minimal side effects, making them suitable for colon-targeted therapy. Chitosan/alginate nanoparticles (CANPs) show potential in the treatment of intestinal diseases due to their strong electrostatic interaction and intestinal mucosal adhesion.
Article
Chemistry, Multidisciplinary
Kyoung Sub Kim, Kun Na, You Han Bae
Summary: Oral administration of pharmaceuticals is challenging due to solubility, size, enzymes, and transporters. Efforts have been made to enhance oral bioavailability using nanoparticles with specific and nonspecific uptake pathways. However, translating results from animal models to humans is difficult and inconsistent. Parameters for translational potential in designing oral nanoparticles are discussed in this review.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Endocrinology & Metabolism
Bo Ahren, Yuichiro Yamada, Yutaka Seino
Summary: The insulin response to oral glucose is influenced by incretin hormones such as GLP-1 and GIP. Studies on GIP and GLP-1 receptor knockout mice have shown varying results regarding glucose tolerance following oral glucose administration. Further investigations on mice with genetic deletions of both receptors suggest a potential dependence of incretin hormone relevance on glucose load, with GIP receptor KO mice exhibiting glucose intolerance after large glucose loads but not smaller ones, while GLP-1 receptor KO mice show normal glucose tolerance with an increased insulin response.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Patrik Lundquist, Georgiy Khodus, Zhigao Niu, Lungile Nomcebo Thwala, Fiona McCartney, Ivailo Simoff, Ellen Andersson, Ana Beloqui, Aloise Mabondzo, Sandra Robla, Dominic-Luc Webb, Per M. Hellstroem, asa Keita, Eduardo Sima, Noemi Csaba, Magnus Sundbom, Veronique Preat, David J. Brayden, Maria Jose Alonso, Per Artursson
Summary: Peptide drugs are not orally bioavailable due to their poor stability and permeability, but nanoparticle formulations may provide a solution. In this study, the absorption mechanisms of four insulin-loaded arginine-rich nanoparticles were investigated. One nanoparticle showed significant uptake and is proposed for further investigation for local delivery.
Article
Chemistry, Multidisciplinary
Lisa R. Volpatti, Delaney M. Burns, Arijit Basu, Robert Langer, Daniel G. Anderson
Summary: This study presents the development of electrostatic complexes for glucose-responsive insulin delivery, demonstrating the potential to mimic healthy mice's glycemic profile in diabetic animals.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Melissa Kanzelberger Coolich, Olivia L. Lanier, Ethan Cisneros, Nicholas A. Peppas
Summary: This work demonstrates the great potential of H-bonded, pH-sensitive P(MAA-g-EG) gels as protein carriers. The use of poly(ethylene glycol) (PEG) chains promotes mucoadhesion in the small intestine, increasing the likelihood of drug release within the absorptive intestinal wall. PEGylation of insulin protects the drug from degradation and improves its stability. Cellular transport and changes in transepithelial resistance caused by PEGylated insulin (PI) were analyzed using a co-culture of Caco-2 and HT-29-MTX cells. The in vivo absorption of insulin in rats confirmed the biological activity of PEGylated insulin conjugates.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Yanfang Wang, Pengbo Chen, Wei Liu, Xiangqian Wei, Juan Zhang, Xinwei Wei, Yun Liu, Luxuan Rao, Shiming Zhang, Jicheng Yu, Xiao Ye, Jinqiang Wang, Zhen Gu
Summary: Researchers have developed a novel insulin nanocarrier system that can effectively and safely control blood glucose levels in type 1 diabetes patients for an extended period of time.
Article
Pharmacology & Pharmacy
Amani Elsayed, Mayyas Al-Remawi, Nisrein Jaber, Khalid M. Abu-Salah
Summary: Diabetes mellitus is a metabolic endocrine disease characterized by chronic hyperglycemia. Insulin therapy is crucial for its management, with subcutaneous administration being the most common route. To improve oral insulin absorption, novel approaches in the field of nanoscience and nanotechnology have been explored, including the use of nanoparticles, nano-platforms, and nanocarriers.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Lijun Cai, Guopu Chen, Lingyu Sun, Shuangshuang Miao, Luoran Shang, Yuanjin Zhao, Lingyun Sun
Summary: A novel micromotor delivery system with rocket-like architecture and effervescent-tablets-derived fuel was developed for efficient oral macromolecule delivery. The micromotors, inspired by rockets, feature sharp needle tips for loading and efficient penetrating, and tail wings for loading effervescent powders and avoiding perforation. When exposed to a water environment, the effervescent fuel generates CO2 bubbles to propel the micromotors, allowing them to inject into the surrounding mucosa for drug release. These micromotors showed efficacy in regulating blood sugar glucose in a diabetic rabbit model, indicating their potential for clinical oral delivery of macromolecules.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Meng-Fan Li, Lang Chen, Ming-Ze Xu, Jing-Lin Zhang, Qin Wang, Qing-Zhu Zeng, Xing-Chuan Wei, Yang Yuan
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Engineering, Environmental
Yi Zhang, Jian Li, Zhen Wang, Ming-Ze Xu, Zhi Zeng, Jin-peng Huang, Yan-Qing Guan
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Materials Science, Biomaterials
Li Liu, Mingchao Li, Mingze Xu, Zhen Wang, Zhi Zeng, Yunqing Li, Yi Zhang, Rong You, Chu-Hua Li, Yan-Qing Guan
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Materials Science, Biomaterials
Mingchao Li, Kaikai Hu, Danmin Lin, Zhen Wang, Mingze Xu, Jinpeng Huang, Zhan Chen, Yi Zhang, Liang Yin, Rong You, Chu-Hua Li, Yan-Qing Guan
Summary: The study developed a degradable nano-MgO micelle composite with double interference using RNAi and mRNA to target the alpha-synuclein gene, showing significantly improved inhibitory effects in treating Parkinson's disease. Cell experiments demonstrated increased viability in the PD cell model and the ability of the composite to penetrate the blood brain barrier and deliver genes and mRNA to neurons.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)