Article
Fisheries
Rui Xia, Qiang Hao, Yadong Xie, Qingshuang Zhang, Chao Ran, Yalin Yang, Wenhao Zhou, Fuwei Chu, Xiaomei Zhang, Yu Wang, Zhen Zhang, Zhigang Zhou
Summary: Saccharomyces cerevisiae supplementation improves the growth, intestinal and liver health, and disease resistance of channel catfish.
AQUACULTURE REPORTS
(2022)
Review
Immunology
Olga Dremova, Maximilian Mimmler, Nadja Paeslack, My Phung Khuu, Zhenling Gao, Markus Bosmann, Lucien P. Garo, Nathalie Schoen, Alexa Mechler, Yunes Beneich, Vivian Rebling, Amrit Mann, Giulia Pontarollo, Klytaimnistra Kiouptsi, Christoph Reinhardt
Summary: In this article, the basic principles of germ-free isolator technology, various sterilization methods, and sterility testing methods for monitoring germ-free mouse colonies are reviewed. The effectiveness and limitations of these methods are discussed, along with the possible sources of isolator contamination and an overview of reported contaminants.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hua Zhou, Jing Sun, Bing Yu, Zuohua Liu, Hong Chen, Jun He, Xiangbing Mao, Ping Zheng, Jie Yu, Junqiu Luo, Yuheng Luo, Hui Yan, Liangpeng Ge, Daiwen Chen
Summary: This study investigated the interactions between host and microbiota in a germ-free (GF) pig model, and explored the effects of maternal gut microbiota transplantation on the growth and intestinal functions of newborns. The results showed that the absence of gut microbes impaired growth and nutrient digestibility, while healthy sow gut microbiota transplantation improved growth and nutrient digestibility, indicating the importance of intestinal microbiota for intestinal development and functions.
Article
Fisheries
Yuan-Yuan Yao, Rui Xia, Ya-Lin Yang, Chen-Chen Gao, Feng-Li Zhang, Qiang Hao, Chao Ran, Zhen Zhang, Zhi-Gang Zhou
Summary: The study demonstrated that AiiO-AIO6 could significantly enhance the growth performance of zebrafish, decrease feed conversion ratio, and increase weight and growth rate. Additionally, AiiO-AIO6 was able to alter intestinal microbiota structure and enhance the content of beneficial short-chain fatty acids.
AQUACULTURE REPORTS
(2022)
Article
Immunology
Yapeng Yang, Jinhui He, Yuqing Wang, Lifeng Liang, Zeyue Zhang, Xiang Tan, Shiyu Tao, Zhifeng Wu, Miaomiao Dong, Jixia Zheng, Hang Zhang, Shuaifei Feng, Wei Cheng, Qiyi Chen, Hong Wei
Summary: Whole intestinal microbiota transplantation (WIMT) is more effective than fecal microbiota transplantation (FMT) in the treatment of inflammatory bowel disease (IBD). WIMT can replicate the community structure more accurately and reduce the inflammatory response of the host, alleviating the symptoms of IBD.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Environmental Sciences
Jing Wang, Chen Liu, Shu-Ping Wang, Tian-Xu Zhang, Jing-Yi Chen, Quan Zhou, Yin Hou, Zhen-Guang Yan
Summary: This study investigated the toxic effects and underlying mechanisms of decabromodiphenyl ether (BDE-209) exposure on the intestinal tract using adult male zebrafish as a model. The results showed that BDE-209 exposure caused damage to intestinal structure and barrier function, and promoted oxidative stress, inflammatory response, apoptosis, and disorders in steroid and lipid metabolism. Mechanistically, BDE-209 induced intestinal inflammation through the NF kappa B signaling pathway, and might induce apoptosis through the p53-Bax/Bcl2-Caspase3 pathway. BDE-209 also disrupted cholesterol synthesis and caused lipid metabolism disorders. Furthermore, BDE-209 stress reduced the richness and diversity of intestinal microbiota and decreased the abundance of probiotics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Wenhui Qiu, Tang Liu, Xinjie Liu, Honghong Chen, Shusheng Luo, Qiqing Chen, Jason T. Magnuson, Chunmiao Zheng, Elvis Genbo Xu, Daniel Schlenk
Summary: The immunosuppressive effects of antibiotics and their association with the intestinal microbiota have been studied in zebrafish. The results showed that treatment with enrofloxacin resulted in a decrease in the diversity, richness, and evenness of the intestinal flora in zebrafish. The abundance of certain gut bacteria was significantly correlated with immunosuppressive biomarkers. The study also found that the immunosuppressive effects of enrofloxacin were mediated through alterations of the intestinal microbiome in zebrafish.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Isabelle E. Logan, Natalia Shulzhenko, Thomas J. Sharpton, Gerd Bobe, Kitty Liu, Stephanie Nuss, Megan L. Jones, Cristobal L. Miranda, Stephany Vasquez-Perez, Jamie M. Pennington, Scott W. Leonard, Jaewoo Choi, Wenbin Wu, Manoj Gurung, Joyce P. Kim, Malcolm B. Lowry, Andrey Morgun, Claudia S. Maier, Jan F. Stevens, Adrian F. Gombart
Summary: Xanthohumol (XN) requires the intestinal microbiota to mediate its benefits in improving glucose and lipid metabolism, with changes in both microbial composition and functional capacity. The study found that XN supplementation decreased plasma insulin concentrations and improved insulin resistance in conventional mice, but not in germ-free mice. Furthermore, analysis indicated that XN supplementation altered microbial composition and led to the production of biologically active compounds by the intestinal microbiota.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Dang Quang Hieu, Bui Thi Bich Hang, Jep Lokesh, Mutien-Marie Garigliany, Do Thi Thanh Huong, Duong Thuy Yen, Pham Thanh Liem, Bui Minh Tam, Dao Minh Hai, Vo Nam Son, Nguyen Thanh Phuong, Frederic Farnir, Patrick Kestemont
Summary: In this study, the intestinal microbiota of juvenile striped catfish exposed to a salinity gradient was investigated. It was found that the diversity of the intestinal microbiota decreased with increasing salinity, and certain microbial genera were affected differently. Furthermore, the expression of certain genes associated with osmoregulation and stress responses was modified in the fish intestine. These findings indicate that the intestinal microbiota and gene expression play important roles in the adaptation of fish to hyperosmotic environments.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Yongjie Xu, Yongpeng Yu, Qin Zhou, Yang Xiang, Lixuan Chen, Yaxian Meng, Xudong She, Fei Zou, Xiaojing Meng
Summary: Cadmium is a harmful environmental pollutant that damages the nervous system and disrupts the gut microbiota. However, its relationship with neurotoxicity and microbiota alterations is not well understood. This study used a germ-free zebrafish model and found that Cd-induced neurotoxic effects were weaker in germ-free zebrafish. RNA sequencing revealed that Cd-treated conventionally reared zebrafish exhibited decreased expression levels of V-ATPase family genes, which was avoided in germ-free zebrafish. Overexpression of atp6v0cb partially rescued Cd-induced neurotoxicity. These findings suggest that gut microbiota disturbance exacerbates Cd-induced neurotoxicity through genes in the V-ATPase family.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Biochemistry & Molecular Biology
Hui Xia, Huimin Chen, Xue Cheng, Mingzhu Yin, Xiaowei Yao, Jun Ma, Mengzhen Huang, Gang Chen, Hongtao Liu
Summary: Zebrafish is a low-cost and genetically tractable vertebrate model that can be used to investigate the interaction between gut microbiota and host. It has features such as high fecundity, external fertilization, and early optical transparency, making it a valuable tool for addressing research questions not easily addressed in other animal models.
MOLECULAR MEDICINE
(2022)
Article
Immunology
Hiyori Sakaguchi, Yuna Sato, Ryo Matsumoto, Joe Gomikawa, Namie Yoshida, Tomohiro Suzuki, Masaru Matsuda, Norimasa Iwanami
Summary: This study investigated the interaction between the host immune system and intestinal microorganisms in medaka, an established fish model. The results showed that immunodeficiency affected the composition of the microbiota, while gut bacteria influenced intestinal epithelial development and immune responses in medaka. These findings highlight the importance of reciprocal interactions between the microbiome and the intestinal tract for the maturation of the immune system in medaka.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hua Zhou, Bing Yu, Jing Sun, Hong Chen, Zuohua Liu, Liangpeng Ge, Daiwen Chen
Summary: This experiment investigated the impact of gut microbiota on neonatal diarrhea using a germ-free pig model. The results showed that the absence of gut microbiota may cause diarrhea, while the transplantation of maternal fecal microbiota can reverse this condition.
ANIMAL BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Pan-Pan Jia, Yi-Fan Yang, Muhammad Junaid, Huang-Jie Jia, Wei-Guo Li, De-Sheng Pei
Summary: Phages are unique viruses that can specifically infect bacteria and play important roles in microbial regulation. This review explores the methodology and functions of intestinal microbiota in the host, proposing the use of phages to infect and reduce/eliminate gut bacteria in zebrafish models. It also highlights the potential of phage therapy to control intestinal microbiota and prevent bacteria-related human diseases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Anran Wang, Zhen Zhang, Qianwen Ding, Yalin Yang, Jerome Bindelle, Chao Ran, Zhigang Zhou
Summary: Fish have limited capability to utilize carbohydrates compared to mammals. In this study, zebrafish fed with omnivorous and herbivorous diets showed better glucose homeostasis due to enrichment of the commensal bacterium Cetobacterium somerae, which produces acetate. Dietary supplementation of acetate promotes glucose utilization in zebrafish, and the beneficial effect of acetate and C. somerae on glucose homeostasis is mediated through parasympathetic activation. These findings suggest potential strategies for improving fish carbohydrate utilization.
