Expanding PAM recognition and enhancing base editing activity of Cas9 variants with non‐PI domain mutations derived from xCas9

Continuous evolution of SpCas9 variants compatible with non-G PAMs

(2020) Shannon M. Miller et al.   NATURE BIOTECHNOLOGY

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation

(2020) Mateusz Legut et al.   Cell Reports

Precision genome engineering in rice using prime editing system

(2020) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope

(2020) Ziying Hu et al.   PLOS BIOLOGY

Structural insights into a high fidelity variant of SpCas9

(2019) Minghui Guo et al.   CELL RESEARCH

Precise therapeutic gene correction by a simple nuclease-induced double-stranded break

(2019) Sukanya Iyer et al.   NATURE

Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors

(2019) Xinyi Liu et al.   Molecular Therapy-Nucleic Acids

Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease

(2019) Weizhong Chen et al.   PLOS BIOLOGY

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Predictable and precise template-free CRISPR editing of pathogenic variants

(2018) Max W. Shen et al.   NATURE

Protospacer Adjacent Motif-Induced Allostery Activates CRISPR-Cas9

(2017) Giulia Palermo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural insights into DNA cleavage activation of CRISPR-Cas9 system

(2017) Cong Huai et al.   Nature Communications

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9

(2016) Carolin Anders et al.   MOLECULAR CELL

Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

(2016) Seiichi Hirano et al.   MOLECULAR CELL

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

(2016) Dominik Paquet et al.   NATURE

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

The structural biology of CRISPR-Cas systems

(2015) Fuguo Jiang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE