Article
Chemistry, Physical
Yun He, Yu Peng, Lishuang Liu, Sha Hou, Junyu Mu, Liang Lan, Lijia Cheng, Zheng Shi
Summary: This study compares the bone induction of five types of calcium phosphate biomaterials implanted in mice and explores the mechanisms of vascularization and particle-size-related osteoinduction. The results show that nanoscale Ca-P biomaterials can induce osteogenesis in mice muscle, with TCP showing higher osteoinductive effects compared to HA. The particle size of the biomaterials affects angiogenesis and osteogenesis, and there is a positive correlation between the number of blood vessels and the area percentage of new bone tissue.
Article
Biochemical Research Methods
Liu Zhongxing, Wu Shaohong, Li Jinlong, Zhang Limin, Wang Yuanzheng, Gao Haipeng, Cao Jian
Summary: The study successfully developed a hydroxyapatite scaffold with efficient osteoinduction and antimicrobial activity, utilizing surface-specific binding of peptides. This scaffold showed improved cell proliferation and adhesion, as well as enhanced expression of key bone formation markers. The scaffold also exhibited strong antibacterial ability against common pathogens.
JOURNAL OF BIOLOGICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Eamon J. Sheehy, Gregory J. Miller, Isabel Amado, Rosanne M. Raftery, Gang Chen, Kai Cortright, Arlyng Gonzalez Vazquez, Fergal J. O'Brien
Summary: By leveraging the differential response of genes to mechanical loading, novel therapeutics can be identified. Placental growth factor (PGF) has been established as a mechanically augmented gene promoting angiogenesis and osteogenesis. A mechanobiology-informed approach was executed to design a scaffold for controlled delivery of PGF, demonstrating promising results in promoting regeneration of critically-sized bone defects.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biotechnology & Applied Microbiology
Elizaveta Kon, Francesca Salamanna, Giuseppe Filardo, Berardo Di Matteo, Nogah Shabshin, Jonathan Shani, Milena Fini, Francesco Perdisa, Annapaola Parrilli, Simone Sprio, Andrea Ruffini, Maurilio Marcacci, Anna Tampieri
Summary: The study investigated the use of biomorphic calcium phosphate bone scaffolds for the regeneration of load-bearing segmental bone defects, showing that the scaffolds promoted new bone formation and quality, with GB-1 scaffold exhibiting higher fracture strength and stability. The scaffolds also demonstrated increased levels of osteoid surface, osteoblast surface, and vessels/microvessels numbers compared to allograft, highlighting their potential for orthopedic applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Huajun Wu, Ya Gao, Lu Xiao, Qin Wei, Naiyin Zhang, Zhi Su, Chuang Ma, Tingjun Ye, Yingbo Wang
Summary: A bioactive PPy@HA/Dex nanocomposite coating with sustained osteoinductivity was successfully constructed on the titanium surface using pulse electrochemical method. The coating showed promising osteogenic ability and angiogenic property during osseointegration of implants.
MATERIALS & DESIGN
(2021)
Article
Nanoscience & Nanotechnology
Mei Fu, Jun Li, Mingchong Liu, Chensong Yang, Qidong Wang, Hongrui Wang, Bingdi Chen, Qingge Fu, Guixin Sun
Summary: We designed a nanocomposite hydrogel with osteoimmunomodulatory and osteoinductive activities to promote osteogenesis of bone marrow stem cells. The hydrogel exhibited desirable mechanical strength, stability, porosity, and biocompatibility. In addition, the combination of sericin and nanohydroxyapatite showed synergistic effects on bone regeneration, enhancing osteogenic differentiation in rat BMSCs.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Shuting Guo, Chuanglong He
Summary: A 3D bioprinted scaffold containing CGRP and PRN is proposed as a new method for effective repair of bone defects. The addition of CGRP and PRN loaded mesoporous silica nanoparticles into a hybrid bio-ink promotes migration and tube formation of HUVECs, aiding in the formation of a new capillary network during bone repair. The release of CGRP simulates the secretion of neuropeptides by sensory nerves, and PRN inhibits the binding process of catecholamine to beta-adrenergic receptor, co-promoting osteogenic differentiation of BMSCs and enhancing bone repair in a rat model.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Hao Huang, Anchun Yang, Jinsheng Li, Tong Sun, Shangke Yu, Xiong Lu, Tailin Guo, Ke Duan, Pengfei Zheng, Jie Weng
Summary: This study improved the porosity and crystallinity of porous hydroxyapatite scaffolds by adjusting the solid content and sintering temperature. Further mineralization significantly enhanced the biological activity of the composite scaffold. In vivo results showed that the multigradient scaffold filled with mineralized hydroxyapatite spheres exhibited higher vascularization ability and a continuous decrease in osteogenic capability over time.
REGENERATIVE BIOMATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Qin Zhao, Miusi Shi, Chengcheng Yin, Zifan Zhao, Jinglun Zhang, Jinyang Wang, Kailun Shen, Lingling Zhang, Hua Tang, Yin Xiao, Yufeng Zhang
Summary: The study reveals that biomaterials, such as biphasic calcium phosphate scaffolds, can promote osteogenesis by modulating the immune responses of macrophages and dendritic cells. This demonstrates that specific regulatory capacities for immune cells in biomaterials can control early inflammatory responses of implanted materials and induce bone formation.
NANO-MICRO LETTERS
(2021)
Article
Materials Science, Biomaterials
Ketki Holkar, Vaijayanti Kale, Ganesh Ingavle
Summary: One of the objectives of bone tissue engineering is to produce scaffolds that mimic the structure and function of natural bone extracellular matrix, and attract and differentiate mesenchymal stem cells into osteoblasts. This study investigated the use of mineralized microenvironments in hydrogel platforms to guide stem cell fate. Results showed that both direct encapsulation and sustained release of nano-hydroxyapatite (nHAp) enhanced osteogenesis, but direct loading of nHAp into the hydrogel increased its mechanical strength and swelling ratio. Biochemical and molecular studies also demonstrated improved osteoinductive and osteoconductive potential. This cost-effective and simple approach has potential applications in clinical settings.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Bing Guo, Xiaodong Feng, Yun Wang, Xiansong Wang, Yue He
Summary: The study evaluated a biomimetic baicalin-incorporating graphene oxide-demineralized bone matrix hybrid scaffold for bone regeneration. It demonstrated that the scaffold enhanced beneficial cross-talk among bone cells and inflammatory cells, which could be utilized as an effective strategy for bone regeneration. Additionally, the scaffold showed potential in transforming inflammatory M1 macrophages into pro-healing M2 macrophages, promoting in vitro angiogenesis and osteogenesis.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Physical
Xiao Li, Ning He, Xiaojing Li, Xu Wang, Lei Zhan, Jialin Song, Wei-En Yuan, Yuanming Ouyang
Summary: A conductive GDY nanomaterial-loaded PCL scaffold was prepared and evaluated for its biocompatibility and effectiveness in peripheral nerve regeneration. The GDY/PCL scaffold significantly improved the proliferation, adhesion, and glial expression of Schwann cells, as well as promoted myelination and axonal growth in a rat sciatic nerve defect model. Additionally, upregulation of vascular factor expression suggested a potential role in angiogenesis for nerve repair.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Engineering, Biomedical
Ketki Holkar, Vaijayanti Kale, Prasad Pethe, Ganesh Ingavle
Summary: This study developed a cell-free and mechanically strong scaffold for bone tissue engineering by combining osteoinductive extracellular vesicles (EVs) with a mineralized microenvironment. The use of MC3T3-EVs at a specific concentration was shown to enhance osteogenesis in target cells, and this effect was further enhanced by combining with a mineralized microenvironment.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Ileana Ielo, Giovanna Calabrese, Giovanna De Luca, Sabrina Conoci
Summary: This review summarizes recent advances in HA-based biocomposites for bone regeneration, including the commonly used inorganic matrices, polymers, and crosslinkers. Research shows that HA composites are generally biocompatible, although results from clinical studies on humans sometimes remain controversial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
