Journal
BRAIN BEHAVIOR AND IMMUNITY
Volume 101, Issue -, Pages 214-230Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2022.01.003
Keywords
Rheumatoid arthritis; Dorsal root ganglia; Lipid signaling; Autoantibodies; Inflammation; Neuropathic pain; Autotaxin; Enpp(2)
Categories
Funding
- Swedish Research Council [542-2013-8373]
- Knut and Alice Wallenberg Foundation [2012.0206, 2018.0161]
- European Research Council (ERC) under the Horizon 2020 research and innovation programme [866075]
- Ragnar Soderberg Foundation [M138/12]
- Konung Gustaf V:s 80-year foundation
- International Association for the Study of Pain John J. Bonica Fellowship
- Canadian Institutes of Health Research fellowship [MFE-171299]
- Swedish Brain Foundation
- Region Uppsala (ALF-grant)
- Uppsala University
- Magnus Bergvall Foundation
- United States Department of Defence [W81XWH-17-1-0455]
- Region Uppsala (RD funds)
- European Research Council (ERC) [866075] Funding Source: European Research Council (ERC)
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Lysophosphatidic acid (LPA) plays an important role in rheumatoid arthritis-associated pain, and its signaling affects joint inflammation and neuropathic pain. In a experimental model, neutralizing antibody and receptor inhibitor reverse pain-like behavior without affecting joint inflammation. LPA synthesizing enzyme is upregulated in dorsal root ganglia (DRG) neurons, and Lpar1 receptor deficiency blocks pronociceptive neurochemical changes in DRG. LPA regulates pain-like behavior through LPA(1) receptor on satellite glia cells (SGCs) in DRG.
Inflammatory and neuropathic-like components underlie rheumatoid arthritis (RA)-associated pain, and lysophosphatidic acid (LPA) is linked to both joint inflammation in RA patients and to neuropathic pain. Thus, we investigated a role for LPA signalling using the collagen antibody-induced arthritis (CAIA) model. Pain-like behavior during the inflammatory phase and the late, neuropathic-like phase of CAIA was reversed by a neutralizing antibody generated against LPA and by an LPA(1/3) receptor inhibitor, but joint inflammation was not affected. Autotaxin, an LPA synthesizing enzyme was upregulated in dorsal root ganglia (DRG) neurons during both CAIA phases, but not in joints or spinal cord. Late-phase pronociceptive neurochemical changes in the DRG were blocked in Lpar1 receptor deficient mice and reversed by LPA neutralization. In vitro and in vivo studies indicated that LPA regulates pain-like behavior via the LPA(1) receptor on satellite glia cells (SGCs), which is expressed by both human and mouse SGCs in the DRG. Furthermore, CAIA-induced SGC activity is reversed by phospholipid neutralization and blocked in Lpar1 deficient mice. Our findings suggest that the regulation of CAIA-induced pain-like behavior by LPA signalling is a peripheral event, associated with the DRGs and involving increased pronociceptive activity of SGCs, which in turn act on sensory neurons.
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