Article
Chemistry, Physical
Ji Hyun Yang, Hyeong Ryeol Kim, Ja Hyun Lee, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study aimed to enhance power output by optimizing electrical communication for GOx on a composite surface and found that immobilized GOx at a concentration of 40:80 mM EDC:NHS exhibited the highest activity with a power density of 2.24 mWcm(-2) under optimal conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Chemistry, Physical
Umran Duru Kamaci, Aysegul Peksel
Summary: Phytase was successfully immobilized into polyvinyl alcohol-sodium alginate (PVA-SA) electrospun nanofiber, leading to enhanced catalytic activity. Characterization of the PVA-SA nanofibers was conducted using SEM, XRD, FT-IR, TG, and DSC techniques, showing a fiber diameter of around 32 nm.
Article
Biophysics
Mottour Vinayagam Chinnamani, Adeela Hanif, Padmanathan Karthick Kannan, Sandeep Kaushal, Muhammad Junaid Sultan, Nae-Eung Lee
Summary: In this study, a hollow microneedle (HMN) based on soft hollow microfibers and a microneedle-integrated microfluidic biosensor patch (MIMBP) capable of integrated blood sampling and electrochemical biosensing of biomolecules are proposed. The HMNs are fabricated by electroplating flexible and mechanically durable hollow microfibers made from a nanocomposite matrix. The MIMBP uses negative pressure to collect blood and deliver it to a flexible electrochemical biosensor modified with gold nanostructures and Pt nanoparticles. This platform has the potential to revolutionize personalized and decentralized healthcare.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Optics
Bian Zhenglan, Qi Yue, Xue Liang, Hu Anduo, Yang Hui, Chu Fenghong
Summary: This study presents a glucose biosensor based on a hydrogel optical fiber that is immobilized with a fluorescence indicator and glucose oxidase (GO). The sensor shows good sensitivity and efficiency, and it performs optimally at a pH range of 4.5-6 and a temperature of 25 degrees C. The glucose concentration can be accurately measured within the range of 20-100 mu g/mL, making it suitable for biomedical applications.
Article
Chemistry, Multidisciplinary
Chuhan Lv, Xuewei Yang, Zongkang Wang, Ming Ying, Qingguo Han, Shuangfei Li
Summary: The research demonstrates that amination modification significantly improves the performance of bioelectrodes, leading to increased enzyme immobilization and activity, enhanced peak current intensity and transfer rate, as well as remarkable electro-activity and favorable reaction at the electrode.
Article
Agricultural Engineering
Mohamed S. Hellal, Enas M. Abou-Taleb, Ahmed M. Rashad, Gamal K. Hassan
Summary: In this study, the immobilization of anaerobic sludge in sodium alginate (SA) with polyaniline (PANI) nanoparticles (NPs) was found to enhance the degradation of organic matter in dairy wastewater (DWW) and increase biohydrogen yield. The concentration of PANI NPs in SA directly affected the degradation in DWW and bio-hydrogen production.
BIOMASS & BIOENERGY
(2022)
Article
Pharmacology & Pharmacy
Zhimin Luo, Guoning Chen, Ke Yang, Lu Wang, Xia Cui, Jiameng Xu, Qiang Fu
Summary: A method without organic solvents and catalysts was developed to synthesize silica-encapsulated glucose-oxidase-coated magnetic nanoparticles through self-assembly. The encapsulated enzyme showed good linear performance and recoveries in detecting glucose in urine samples. This study demonstrates the potential of the self-assembly method and provides a promising tool for glucose detection and diabetes monitoring.
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Physical
Eimantas Ramonas, Atefeh Shafaat, Marius Dagys, Tautgirdas Ruzgas, Dalius Ratautas
Summary: This study investigated the catalytic properties of citrate-capped gold nanoparticles and gold nanoparticle-protein conjugates, showing that gold nanoparticles form stable conjugates with proteins but do not increase enzyme activity. Under certain conditions, gold nanoparticles can mimic enzymatic reactions, emphasizing the need for specific measurements to differentiate between enzyme and gold nanoparticle-catalyzed reactions.
JOURNAL OF CATALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
Tyrell J. Williams, Claudia Daboin, Paul Kim, Fengguang Guo, Paul de Figueiredo, Daniel L. Alge
Summary: Methods for culturing oxygen-sensitive cells and organisms under anaerobic conditions are vital to biotechnology research. This study developed a biomaterial-based platform for anaerobic culture using glucose oxidase (GOX) functionalized alginate microparticles. These microparticles had high enzyme activity and were able to rapidly deplete oxygen, creating a suitable environment for anaerobic culture.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Energy & Fuels
Mahvash Rahdar, Masoud Madadelahi, Mohammadmehdi Namazi
Summary: This study investigates the characteristics of generating microfibers using centrifugal microfluidic devices, and finds that droplets can transition into microfibers within a specific range of rotational speed. The results demonstrate that microfiber diameter can be controlled by adjusting polymer concentration and micronozzle size, and the resulting grooved microfibers can be used as scaffolds for muscle growth and regeneration in tissue engineering.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2023)
Article
Materials Science, Multidisciplinary
Tanusree Sarkar, Nandini Mukherjee, Jayoti Das
Summary: This paper studies a conductive glucose sensor using glucose oxidase immobilized macro porous silicon. The macro porous silicon layer is grown on a p-type silicon substrate through an electrochemical etching process. Two silver electrodes are made on the front side of the macro porous silicon layer. The sensor shows good response, high sensitivity, and excellent repetitive behavior, and different conduction mechanisms dominate at different applied field ranges.
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
(2022)
Article
Biotechnology & Applied Microbiology
Laila Hossain, Marisa De Francesco, Patricia Tedja, Joanne Tanner, Gil Garnier
Summary: This study presents a new generation of nanocellulose coated-paper sensors for measuring glucose concentration in blood. The sensors are rapid, easy to use, and robust, and utilize nanocellulose gel to store and stabilize enzymes. The sensors produce a color change from red to orange in response to changes in blood glucose concentration. The prototype device designed based on these sensors can instantly measure glucose concentration from blood.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Minrui Ou, Wanying Li, Zihui Zhang, Xiaoping Xu
Summary: This study developed a novel biosorbent using sodium alginate, beta-cyclodextrin, and diatomite, which demonstrated low cost, easy availability, simple preparation process, and environmental friendliness. The biosorbent showed significant selectivity for uranium(VI) adsorption, with high adsorption capacity and excellent reusability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biophysics
Changkyu Lee
Summary: In this study, a GOD-gelatin hydrogel was prepared and demonstrated its potential in suppressing tumor recurrence after breast cancer surgery. It showed anticancer effects through H2O2 generation and remained at the surgical site to continuously suppress tumor recurrence.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Materials Science, Multidisciplinary
Wen Zhang, Wenbo Ye, Yunfeng Yan
Summary: 3D bioprinting, integrating advances in engineering, cell biology, and material science, can fabricate artificial tissues and organ-like structures with biology or clinic-relevant size, shape, and structural integrity. Suitable bioink is crucial in modulating the printing process, properties, and functions of printed constructs.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Wenbo Ye, Guangliang Zhang, Xiaomin Liu, Qidi Ren, Fangqian Huang, Yunfeng Yan
Summary: In this study, polysaccharide-stabilized zein nanoparticles were successfully prepared using a novel flash nanoprecipitation method. These nanoparticles exhibited small size, good stability, and excellent drug encapsulation efficiency. Moreover, they showed sustained release of the drug, making them promising for sustainable delivery of hydrophobic drugs.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fengfan Liu, Huahui Su, Mengqian Li, Wanxuan Xie, Yunfeng Yan, Qi Shuai
Summary: Polyethylenimine (PEI) is commonly used for gene delivery, but its high cytotoxicity and non-specific protein adsorption limit its efficacy and practical applications. In this study, hydrophobically modified and zwitterion-modified PEIs were synthesized and demonstrated to be efficient gene carriers. These modified PEIs showed excellent siRNA binding capacity, reduced cytotoxicity, nonspecific protein adsorption, and enhanced stability. The findings suggest that zwitterionic modification is an effective method to improve the performance of polycationic gene carriers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Yunfeng Yan, Guangliang Zhang, Chengfan Wu, Qidi Ren, Xiaomin Liu, Fangqian Huang, Yi Cao, Wenbo Ye
Summary: RNA interference (RNAi) is a promising approach to treat genetic diseases, and functional polymers can be effective vehicles for delivering small interfering RNA (siRNA) required for this therapy. This study synthesized a series of polymers with controllable structures and narrow distributions, and discovered effective carriers for siRNA delivery. The hydrophobicity, charge density, and pKa values of the polymers influence the delivery efficiency.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Hua-Tao Liu, Chun-Yue Weng, Shen-Yuan Xu, Shu-Fang Li, Ya-Jun Wang, Yu-Guo Zheng
Summary: A novel enzyme engineering strategy was developed in this study to modify carbonyl reductase LsCR, resulting in increased catalytic activity and efficiency.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Wenbo Ye, Fangtao Zhu, Yue Cai, Longyu Wang, Guangliang Zhang, Guangkuo Zhao, Xiaohe Chu, Qi Shuai, Yunfeng Yan
Summary: A novel nanoparticle platform was developed to improve the stability and delivery efficacy of paclitaxel-loaded nanoparticles (NPs), showing excellent anti-tumor efficacy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Yunfeng Yan, Fangtao Zhu, Huahui Su, Xiaomin Liu, Qidi Ren, Fangqian Huang, Wenbo Ye, Mengdan Zhao, Yunchun Zhao, Junpeng Zhao, Qi Shuai
Summary: This study constructed a library of 144 functional triblock polymers and discovered three effective carriers for siRNA delivery through high-throughput screening. In vitro evaluation showed that the siRNA-loaded nanoparticles fabricated with these polymers effectively silenced luciferase genes with low cytotoxicity. The chemical structure of the polymers significantly affected the physicochemical properties and in vitro delivery efficacy of the siRNA-loaded nanoparticles. In vivo experiments demonstrated that siRNA nanoparticles based on a specific polymer accumulated at tumor sites, suggesting the potential of this polymer in improving blood circulation and biodistribution of siRNA nanoparticles. This functional triblock polymer platform holds great potential in siRNA-based cancer therapies.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Hua-Tao Liu, Chun-Yue Weng, Lei Zhou, Hao-Bo Xu, Zhen-Yu Liao, Han-Yue Hong, Yuan-Fan Ye, Shu-Fang Li, Ya-Jun Wang, Yu-Guo Zheng
Summary: In this study, protein engineering was used to enhance the catalytic efficiency and thermal stability of CR enzyme. The mutant enzyme LsCR(M4) showed improved performance in bioreduction of carbonyl substrates in both organic phase and neat substrate reaction system.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Qidi Ren, Lu Wang, Wenfei Qian, Baiqiu Chen, Qi Shuai, Yunfeng Yan
Summary: RNA interference (RNAi) holds promise for disease treatments, but the development of safe and effective delivery carriers remains a major challenge. Organic-inorganic hybrid nanoparticles (NPs) integrating functions from distinct materials show great potential in small interfering RNA (siRNA) delivery. In this study, pH responsive amorphous calcium carbonate NPs (ACC NPs) are prepared using flash nanoprecipitation and hybrid NPs are constructed by coating ACC NPs with polyethyleneimine (PEI) for efficient siRNA delivery. The PEI/ACC NPs exhibit robust pH responsiveness and stability, as well as effective siRNA loading and protection, enabling enhanced cellular uptake and efficient endosomal escape for improved siRNA delivery.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Biomedical
Yunfeng Yan, Xiaomin Liu, Longyu Wang, Chengfan Wu, Qi Shuai, Yanmei Zhang, Shuai Liu
Summary: A combinatorial library of 119 novel lipids was constructed to tackle the challenge of mRNA delivery efficiency. Several synthetic lipids with superior mRNA delivery efficacy were identified through in vitro screening. The delivery capability of these lipids was verified in mice models, and the results demonstrated their potential in cancer immunotherapy.
Article
Biotechnology & Applied Microbiology
Chen Dai, Hai-Xing Cao, Jia-Xin Tian, Yan-Chi Gao, Hua-Tao Liu, Shen-Yuan Xu, Ya-Jun Wang, Yu-Guo Zheng
Summary: In this study, the catalytic activity and stereoselectivity of a NADPH-dependent AKR from Kluyveromyces dobzhanskii (KdAKR) towards (5S)-CHOH were improved by mutating its residues in the loop regions around the substrate-binding pocket. The best mutant M6 exhibited a 67-fold higher catalytic efficiency compared to the wild-type KdAKR and improved R-selectivity toward (5S)-CHOH. Moreover, M6 showed a 6.3-fold increase in half-life at 40℃ compared to the wild-type. Under optimal conditions, M6 completely converted 200 g/L (5S)-CHOH to diastereomeric pure (3R, 5S)-CDHH within 8.0 h, with a space-time yield of 300.7 g/L/day.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Shen-Yuan Xu, Lei Zhou, Ying Xu, Han-Yue Hong, Chen Dai, Ya-Jun Wang, Yu-Guo Zheng
Summary: Structural information can assist in engineering enzymes by targeting specific amino acids in particular regions for functional reconstruction. This can improve catalytic performance, including activity, stereoselectivity, and thermostability. Proper selection of target sites based on structure-function relationships is crucial for structure-based design. This article summarizes the effects of residue mutations in different regions on fine-tuning enzyme catalytic performance and discusses the impact of altering the local structural environment on function. Additionally, recent progress in structure-based approaches for enzyme engineering is reviewed to provide guidance on leveraging structural information.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Computer Science, Hardware & Architecture
Junfan Wang, Yi Chen, Yeting Gu, Yunfeng Yan, Qi Li, Mingyu Gao, Zhekang Dong
Summary: In this paper, a lightweight vehicle mounted multi-scale traffic sign detector named AFPN is proposed. The proposed detector utilizes the attention fusion algorithm and improves the feature extraction and information retention capabilities. It also introduces a multi-head detection structure to improve the detection accuracy. Experimental results demonstrate the superior performance of the proposed method in terms of detection accuracy and speed, and it has been successfully deployed on the Jetson Xavier NX for real-time traffic sign detection.
JOURNAL OF SUPERCOMPUTING
(2023)
Article
Biotechnology & Applied Microbiology
Shu-Fang Li, Shen-Yuan Xu, Ya-Jun Wang, Yu-Guo Zheng
Summary: In this study, Pullulanase was modified using a structure-guided consensus approach, resulting in the creation of a quadruple mutant PulAR-A365V/V401C/T504V/H499A. This mutant exhibited enhanced thermostability, pH stability, and catalytic efficiency, making it potentially useful in the starch industry.
BIORESOURCES AND BIOPROCESSING
(2022)