Article
Chemistry, Analytical
Kseniya S. Shin, Shuaiqian Men, Angel Wong, Colburn Cobb-Bruno, Eleanor Y. Chen, Dan Fu
Summary: A multimodal quantitative imaging approach combining SRS, TPF, and SHG microscopy can provide useful diagnostic information for bone tissue, with the potential to separate bone cancer-associated diagnostic categories with an average accuracy of 92%.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yuying Tan, Haonan Lin, Ji-Xin Cheng
Summary: This study demonstrates a high-content hyperspectral stimulated Raman scattering (h(2)SRS) imaging approach that allows for simultaneous mapping of multiple biomolecules at the single-cell level, revealing chemotherapy-induced metabolic reprogramming and unique metabolic features of chemoresistance. This approach could aid in the discovery of therapeutic targets to combat chemoresistance.
Article
Biochemistry & Molecular Biology
Jiajun Du, Haomin Wang, Lu Wei
Summary: As an emerging optical imaging modality, stimulated Raman scattering (SRS) microscopy provides valuable opportunities for chemical biology studies due to its rich chemical information. The development of Raman probes using the chemical biology toolbox has played a crucial role in advancing SRS microscopy and expanding biological applications. This perspective discusses the development of biorthogonal SRS imaging using small tagging and isotopes, highlighting their unique advantages for metabolic pathway analysis and microbiology investigations. It also proposes potential opportunities for chemical biology studies integrating small tagging with SRS imaging. Additionally, it summarizes the current designs of highly sensitive and super-multiplexed SRS probes and provides future directions and considerations for next-generation functional probe design, aiming to bridge the gap between SRS microscopy and chemical biology research.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yongqing Zhang, Yihui Zhou, Wen Fang, Hanlin Zhu, Cunqi Ye, Delong Zhang, Hyeon Jeong Lee
Summary: The spatiotemporal dynamics of small-molecule metabolites play essential roles in understanding the fundamental machinery of life. However, the subcellular-level regulatory mechanisms are still poorly understood due to a lack of tools for tracking small-molecule metabolites. In this study, we developed high-resolution stimulated Raman scattering (SRS) imaging of a genetically engineered model (GEM) to map metabolites at subcellular resolution. Using this technique, we discovered an unexpected regulatory mechanism of a critical metabolite, sterol, in yeast. Amplifying the strength of vibrational imaging through genetic modulation, we found that isozymes of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) play a role in promoting ergosterol distribution to specific subcellular locations and enriching it through local HMGR-directed synthesis. These findings provide new insights into sterol metabolism and disease treatment strategies, and demonstrate the potential of SRS-GEM as a platform for studying metabolic regulation and biopharmaceutical research.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemical Research Methods
Rajeev Ranjan, Giovanni Costa, Maria Antonietta Ferrara, Mario Sansone, Luigi Sirleto
Summary: In this study, the relationship between hardware characteristics and image processing in SRS microscopy literature was explored, with a focus on statistical analysis of image noise. The results demonstrated an improvement in image denoising with the adapted singular spectrum analysis method.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Physics, Multidisciplinary
Luca Genchi, Sergey P. Laptenok, Carlo Liberale
Summary: Stimulated Raman scattering (SRS) microscopy is a popular technique due to its linearity and lack of nonresonant background. However, there are other optical processes, such as nonlinear transient scattering and absorption, that can compromise the contrast and sensitivity of SRS.
ADVANCES IN PHYSICS-X
(2023)
Article
Biochemical Research Methods
Moritz Floess, Tobias Steinle, Florian Werner, Yunshan Wang, Willi L. Wagner, Verena Steinle, Betty S. Liu, Yifan Zheng, Zi Chen, Maximilian Ackermann, Steven J. Mentzer, Harald Giessen
Summary: Pectin is a heteropolysaccharide that plays a role in maintaining the structure of plant cell walls. When applied to the surface of mammalian organs, pectin films form a strong bond with the surface glycocalyx. Understanding the water transport dynamics in pectin hydrogels is important for medical applications such as surgical wound sealing.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Analytical
Kideog Bae, Le Xin, Wei Zheng, Carol Tang, Beng-Ti Ang, Zhiwei Huang
Summary: Recent genomic studies have paved the way for effective clinical treatments of glioblastoma (GBM) subtypes, but intratumoral heterogeneity complicates subtype identification. The use of hyperspectral stimulated Raman scattering (SRS) microscopy allows for rapid, label-free molecular assessment of GBM intratumoral heterogeneity. By developing a unique SRS imaging diagnostic platform, it is possible to accurately determine tumor heterogeneity in GBM tissues, thus potentially improving targeted therapy for GBM in the future.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Neville Murphy, William J. Tipping, Henry J. Braddick, Liam T. Wilson, Nicholas C. O. Tomkinson, Karen Faulds, Duncan Graham, Pau Farras
Summary: This study reports the application of spectral phasor analysis to unmix three bioorthogonal Raman probes in live cells. Triplex detection of a metallacarborane, a bis-alkyne, and a deuterated fatty acid can be achieved within the cell-silent region of the Raman spectrum. Additionally, nine regions of interest can be spectrally unmixed from the hyperspectral dataset, offering a new capability for multiplexed Raman imaging of live cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Haonan Lin, Hyeon Jeong Lee, Nathan Tague, Jean-Baptiste Lugagne, Cheng Zong, Fengyuan Deng, Jonghyeon Shin, Lei Tian, Wilson Wong, Mary J. Dunlop, Ji-Xin Cheng
Summary: The study presents a system for acquiring high-resolution SRS spectra using a polygon scanner, while significantly improving signal-to-noise ratio through the use of a spatial-spectral residual learning network. The authors demonstrate high-speed imaging and tracking of multiple biomolecules in the fingerprint region using a polygon-based ultrafast delay scanner and deep learning framework.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Weifeng Zhang, Yun Li, Lin Yao, Fan Wu, Chen Chen, Liqun Zhou, Bo Zheng, Pu Wang, Weili Hong
Summary: A new method based on SRS microscopy was reported to rapidly determine the antimicrobial susceptibility of beta-lactam antibiotics by measuring both the metabolic activity and morphological deformation of bacteria. The evaluation method achieved a 93.2% categorical agreement and a 93.2% essential agreement with the standard reference method when performing AST of cefotaxime on 103 E. coli strains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Minghua Zhuge, Kai-Chih Huang, Hyeon Jeong Lee, Ying Jiang, Yuying Tan, Haonan Lin, Pu-Ting Dong, Guangyuan Zhao, Daniela Matei, Qing Yang, Ji-Xin Cheng
Summary: Stimulated Raman scattering microscopy significantly boosts imaging sensitivity for retinoid molecules, allowing their distribution in various biological systems to be mapped. This label-free chemical imaging tool opens new opportunities for understanding the function of retinoids in biological systems.
Article
Spectroscopy
Christian T. Graefe, David Punihaole, Michael J. Lynch, W. Ruchira Silva, Renee R. Frontiera
Summary: The research team successfully implemented super-resolution techniques based on Raman spectroscopy, achieving signal depletion and spatial resolution enhancement. By adapting the laser system for soft matter samples with increased repetition rate, they demonstrated improved imaging capabilities. However, despite lowering pulse powers, significant improvements in resolution were not achieved.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Hongli Ni, Peng Lin, Yifan Zhu, Meng Zhang, Yuying Tan, Yuewei Zhan, Zian Wang, Ji-Xin Cheng
Summary: This study introduces a compact fiber laser-based multiwindow SRS imaging system that addresses issues with bulky and environmentally sensitive solid-state OPO, and slow wavelength tuning speed, demonstrating high-quality SRS metabolic imaging across various Raman windows.
ANALYTICAL CHEMISTRY
(2021)