Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 16, Pages 18079-18086Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c23209
Keywords
light-activated electrochemistry; cell capture; single-cell isolation; surface chemistry; transparent semiconductor
Funding
- ARC Centre of Excellence in Convergent Bio-Nano Science and Technology [CE140100036]
- ARC Laureate Fellowship [FL150100060]
- Australian Government Research Training Program Scholarship
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In this study, a new photoelectrochemical surface was developed for selectively isolating individual rare cells from complex biological samples. The surface was constructed using a transparent electrode and orthogonal surface chemistry, and the capture and release of single cells were achieved through the co-stimulation of light and electric potential.
Selective isolation of individual target cells from a heterogeneous population is technically challenging; however, the ability to retrieve single cells can have high significance in various aspects of biological research. Here, we present a new photoelectrochemical surface based on a transparent electrode that is compatible with high-resolution fluorescence microscopy for isolating individual rare cells from complex biological samples. This is underpinned by two important factors: (i) careful design of the electrode by patterning discrete Au disks of micron dimension on amorphous silicon-indium tin oxide films and (ii) orthogonal surface chemistry, which modifies the patterned electrode with self-assembly layers of different functionalities, to selectively capture target cells on the Au disks and resist cell binding to the amorphous silicon surface. The co-stimulation of the surface using light from a microscope and an electric potential triggers the reductive desorption of the alkanethiol monolayer from the Au disks to release the single cells of interest from the illuminated regions only. Using circulating tumor cells as a model, we demonstrate the capture of cancer cells on an antibody-coated surface and selective release of single cancer cells with low expression of epithelial cell adhesion molecules.
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