Article
Microbiology
Swapnil Kajale, Neelima Deshpande, Tushar Lodha, Yogesh Shouche, Avinash Sharma
Summary: A new species of halophilic archaeon, Natronococcus pandeyae, was isolated from Sambhar Salt Lake in India. The strain LS1_42(T) showed unique characteristics in its morphology, growth conditions, phylogenetic relationship, and genome properties.
CURRENT MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ya-Ping Sun, Bei-Bei Wang, Zhang-Ping Wu, Xi-Wen Zheng, Jing Hou, Heng-Lin Cui
Summary: Five novel halophilic archaeal strains were isolated from coastal saline sediments and sea salt in China. They demonstrated typical morphology and growth characteristics and showed high similarities with existing species. However, based on genetic and phylogenetic analyses, it is confirmed that these strains represent five new species. This is the first report of a novel haloarchaeon isolated from a marine intertidal zone.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xin-Xin Li, Shun Tan, Mu Cheng, Yao Hu, Xue Ma, Jing Hou, Heng-Lin Cui
Summary: Four halophilic archaea strains were isolated from salt and soda lakes in China. These strains were genetically distinct from existing genera and may represent new species and genera within the family Natrialbaceae. Phenotypic characteristics and lipid profiles also differentiated these strains from related genera.
Article
Biotechnology & Applied Microbiology
Fabian Graessle, Caroline Plugge, Paolo Franchini, Bernhard Schink, David Schleheck, Nicolai Mueller
Summary: In this study, a rhamnose-degrading bacterium isolated from profundal sediment of Lake Constance was identified as a new genus Pelorhabdus. The strain exhibited growth at pH 4.0-7.5 and temperature range of 15-30 degrees C, with fermentation products including acetate, propionate, ethanol, butyrate, and 1-propanol. Genomic comparisons suggested a distinct identity warranting the proposal of the new genus Pelorhabdus and species Pelorhabdus rhamnosifermentans gen. nov., sp. nov.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Afaf Hamame, Reham Magdy Wasfy, Cheikh Ibrahima Lo, Florence Fenollar, Didier Raoult, Pierre-Edouard Fournier, Linda Houhamdi
Summary: Two new bacterial strains, Marseille-P2698(T) and Marseille-P2260(T), were isolated from human stools using the culturomic method. These two strains were fully described using the taxonogenomic approach. Marseille-P2698(T) was identified as a Gram-negative, motile, non-spore-forming, rod-shaped bacterium, while Marseille-P2260(T) was identified as a Gram-positive, motile, spore-forming rod-shaped bacterium.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Janusz Blaszkowski, Khadija Jobim, Piotr Niezgoda, Edward Meller, Ryszard Malinowski, Pawel Milczarski, Szymon Zubek, Franco Magurno, Leonardo Casieri, Wojciech Bierza, Tomasz Blaszkowski, Thomas Crossay, Bruno Tomio Goto
Summary: Examination of fungal specimens collected in the Atlantic rain forest ecosystems of Northeast Brazil revealed many potentially new species of Glomeromycota, with two closely related fungi forming glomerocarps. Phylogenetic analyses indicated that these two fungi represent two divergent undescribed species in the family Glomeraceae, with one placed in the genus Dominikia and the other in a sister clade to the genus Kamienskia.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Zhenqiang Zuo, Dahe Zhao, Jian Zhou, Jing Han, Hua Xiang
Summary: Strain N1521(T) is a novel extremely halophilic archaeon with resistance to salt stress, exhibiting a wide range of adaptability in terms of temperature, pH, and salinity, and capable of producing red pigments.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Danillo Oliveira Alvarenga, Ana Paula Dini Andreote, Luis Henrique Zanini Branco, Endrews Delbaje, Renata Beatriz Cruz, Alessandro de Mello Varani, Marli Fatima Fiore
Summary: The genus Nostoc contributes significantly to carbon and nitrogen availability in terrestrial ecosystems and forms symbiotic relationships with non-diazotrophic organisms. A study identified new Nostoc-like cyanobacterial genera and species in South American ecosystems, highlighting the importance of these regions for harboring unknown microbial diversity in the face of increasing anthropogenic threats.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Hye Seon Song, Juseok Kim, Yeon Bee Kim, Se Hee Lee, Tae Woong Whon, Seong Woon Roh
Summary: Two novel halophilic archaeal strains were isolated from solar salt in South Korea. Through genetic, physiological, and biochemical analyses, they were found to represent a new genus and species in the family Halobacteriaceae.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Microbiology
Tomohiro Watanabe, Tatsuya Yabe, Jackson M. Tsuji, Manabu Fukui
Summary: A novel sulfate-reducing bacterium was isolated from sediment sampled in a brackish lake in Japan, capable of respiratory growth with various electron donors and acceptors. Based on gene sequence similarity, it is closely related to Desulfarculus baarsii 2st14(T). It belongs to the family Desulfarculaceae but does not affiliate with any existing genus.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Microbiology
Heng Zhou, Ming Yang, Qiong Xue, Sumit Kumar, Shengjie Zhang, Jian Zhou, Dahe Zhao, Hua Xiang
Summary: A novel Gram-stain-negative bacterium, named Rhabdonatronobacter sediminivivens, was isolated from the sediment of Hutong Qagan Lake in Inner Mongolia Autonomous Region, China. It showed distinct phylogenetic differences from other species in the family Rhodobacteraceae. Based on its fatty acid composition, polar lipid profiles, and genomic characteristics, it can be classified as a new genus and species.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ahmed Saeedi, Nicola J. Cummings, Denise McLean, Ian F. Connerton, Phillippa L. Connerton
Summary: ASxL5(T) is a novel Gram-stain negative bacterium with unique growth characteristics and morphology, closely related to members of the Oceanospirillaceae.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Shenao Zhang, Feilong Chen, Juntao Ke, Yuling Hao, Ruru Pan, Tao Hong, Yongpei Dai, Shaoxing Chen
Summary: Extracellular proteases of haloarchaea have the ability to adapt to high salt concentrations and can be utilized in industrial or biotechnology applications under hypersaline conditions. However, the diversity of these proteases is largely unknown despite the availability of many sequenced genomes. In this study, the gene encoding the extracellular protease Hly176B from Haloarchaeobius sp. FL176 was cloned and expressed in Escherichia coli, revealing its catalytic triad and unique properties. This research enhances our understanding of extracellular proteases and broadens their potential industrial uses.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Microbiology
Heng- Lin Cui, Xiao- Wei Shi, Xue- Meng Yin, Xiao- Yan Yang, Jing Hou, Lin Zhu
Summary: Two halophilic archaeal strains, Gai3-2(T) and NJ-3-1(T), isolated from salt lake and saline soil samples in PR China, represent novel species of Halobaculum genus. The 16S rRNA gene sequences of these strains showed high similarity but lower than recommended threshold for species delimitation.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Dimitry Y. Sorokin, Michail Yakimov, Enzo Messina, Alexander Y. Merkel, Michel Koenen, Nicole J. Bale, Jaap S. Sinninghe Damste
Summary: A pure culture of alkaliphilic haloarchaeon strain AArc-ST capable of anaerobic growth by carbohydratedependent sulfur respiration was obtained from hypersaline lakes in southwestern Siberia. According to phylogenetic analysis, AArc-ST formed a new genus level branch most related to the genus Natronoarchaeum in the order Halobacteriales. The strain is facultatively anaerobic with strictly respiratory metabolism growing either by anaerobic respiration with elemental sulfur and thiosulfate as the electron acceptors or by aerobic respiration at microoxic conditions. Thiosulfate is reduced partially to sulfide and sulfite. It is a first sulfur-reducing alkaliphilic haloarchaeon utilizing sugars, starch and glycerol as substrates for anaerobic growth. It is extremely halophilic (optimum at 3.5 M total Na+) and obligately alkaliphilic (optimum at pH 9.5). The dominant polar lipids include PG and PGP-Me with the archaeol (C20-C20) or extended archaeol (C20-C25) cores. The dominant respiratory lipoquinone is MK8:8. On the basis of unique physiological properties and results of phylogenetic analysis, the soda lake isolate is suggested to be classified into a novel genus and species Natranaeroarchaeum sulfidigenes gen. nov., sp. nov. (=JCM 34033T = UNIQEM U1000T). Furthermore, on the bases of phylogenomic reconstruction, a new family Natronoarchaeaceae fam. nov. is proposed within the order Halobacteriales incorporating Natranaeroarchaeum and three related genera: Natronoarchaeum, Salinarchaeum and Halostella.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Sara Szuroczki, Gorkhmaz Abbaszade, Karoly Boka, Peter Schumann, Meina Neumann-Schaal, Erika Toth
Summary: A new bacterial strain, named Szabonella alba gen. nov., sp. nov., was isolated from a water sample in Hungary. Phylogenetic analysis revealed that it is distinct from its closest relatives in the family Rhodobacteraceae based on 16S rRNA gene sequences and genome-related indices.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Roohollah Kheiri, Maliheh Mehrshad, Ahmad Ali Pourbabaee, Antonio Ventosa, Mohammad Ali Amoozegar
Summary: Lake Urmia in Iran is a hypersaline environment with a salinity of about 27%. Metagenomic analysis revealed that the microbial community in the lake is dominated by Haloferacaceae family (69.8%), mainly Haloquadratum (59.3%) and Halonotius (9.1%). The bacterial community is mainly composed of Salinibacter ruber (23.3%). Genomic variation analysis showed a high level of SNVs and insertions in Lake Urmia's community, possibly due to transformation caused by the extreme conditions and high ionic concentrations.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Vinicius S. S. Kavagutti, Paul-Adrian Bulzu, Cecilia M. M. Chiriac, Michaela M. M. Salcher, Indranil Mukherjee, Tanja Shabarova, Vesna Grujcic, Maliheh Mehrshad, Vojtech Kasalicky, Adrian-Stefan Andrei, Jitka Jezberova, Jaromir Seda, Pavel Rychtecky, Petr Znachor, Karel Simek, Rohit Ghai
Summary: This study performed high-resolution sampling of the spring bloom in a freshwater reservoir and identified previously unknown microbial taxa using metagenome-assembled genomes. The recovered genomes provide insights into the distributional dynamics and doubling times of microbial lineages during the bloom. Additionally, the study uncovered the presence of aplastidic cryptophytes and giant viruses, offering new understanding of plankton dynamics.
Article
Plant Sciences
Somayeh Rasoli-dogaheh, Mostafa Noroozi, Javad Khansha, Mohammad Ali Amoozegar, Rezvan Rahimi, Mahdi Moshtaghi Nikou, Tomas Hauer
Summary: A study on the diversity of cyanobacteria in the rainwater basin of the Persian Gulf region found a novel taxon, Khargia iranica, on Kharg Island. Similar strains were also found in South Italy and India, leading to the recognition of the new genus Khargia. Different analytical methods were used to identify and classify the new species.
Correction
Multidisciplinary Sciences
Roohollah Kheiri, Maliheh Mehrshad, Ahmad Ali Pourbabaee, Antonio Ventosa, Mohammad Ali Amoozegar
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Maryam Yavari-Bafghi, Maryam Rezaei Somee, Mohammad Ali Amoozegar, Seyed Mohammad Mehdi Dastgheib, Mahmoud Shavandi
Summary: This study investigated the removal of phenol using Small Bioreactor Chambers and CaO2 nanoparticles in continuous-flow sand-packed columns. The results showed that simultaneous biostimulation and bioaugmentation could completely eliminate phenol within 42 days, while natural bioremediation columns still had 80.2% remaining at the end of the experiment. CaO2 injection decreased microbial diversity, with order-level groups such as Rhodobacterales and Xanthomonadales dominating in biostimulation columns. Long-term exposure to phenol shifted the microbial population towards heterotrophic members with the potential for phenol and hydrocarbon degradation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Krzysztof T. Jurdzinski, Maliheh Mehrshad, Luis Fernando Delgado, Ziling Deng, Stefan Bertilsson, Anders F. Andersson
Summary: The crossing of environmental barriers poses adaptive challenges and bacterial communities in freshwater-marine transitions are rarely related to brackish counterparts. This study conducted a phylogenomic analysis of metagenome-assembled genomes to investigate the molecular adaptations facilitating cross-biome transitions. The results showed that cross-biome transitions were rare, ancient, and mostly directed toward the brackish biome, accompanied by systematic changes in proteomes and gene functions.
Review
Microbiology
Elham Lashani, Mohammad Ali Amoozegar, Raymond J. Turner, Hamid Moghimi
Summary: Microorganisms play a crucial role in reducing metalloid contamination risks through their ability to tolerate and detoxify these elements.
Article
Microbiology
Alicia Garcia-Roldan, Rafael R. de la Haba, Blanca Vera-Gargallo, Cristina Sanchez-Porro, Antonio Ventosa
Summary: Metagenomic sequences of prokaryotic microbiota from brine of a crystallizer pond in a saltern in Isla Cristina, southwest Spain, were obtained using Illumina. Haloarchaea and Salinibacter were the most abundant prokaryotes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Environmental Sciences
Elham Lashani, Hamid Moghimi, Raymond J. Turner, Mohammad Ali Amoozegar
Summary: Selenium oxyanions are released into environments from various activities and are present in wastewater in many locations worldwide. Excessive amounts of selenium have negative impacts on living organisms. Halophilic and halotolerant microorganisms were used for selenium oxyanion remediation in wastewater containing salt. Factors such as aeration, carbon sources, competitive electron acceptors, and reductase inhibitors were investigated for their effects on SeO32- removal. The study also explored the remediation of SeO32- in agricultural effluents containing NO3-. The results showed that aerobic conditions with succinate as a carbon source were most effective for SeO32- removal. Certain ions, such as WO42- and TeO32-, decreased the removal percentage, while SO42- and PO43- had minimal interference. NO3- had an adverse effect on SeO32- biotransformation. Consortia of halophilic/halotolerant bacteria and yeasts were able to reduce SeO32- in synthetic agricultural wastewater by 45-53% within 120 hours. This study suggests that these consortia could be used for the bioremediation of selenium-contaminated drainage water.
ENVIRONMENTAL POLLUTION
(2023)
Article
Multidisciplinary Sciences
Atefeh Safarpour, Marzieh Ebrahimi, Seyed Abolhassan Shahzadeh Fazeli, Mohammad Ali Amoozegar
Summary: In recent years, there has been growing interest in the anticancer properties of metabolites from halophilic microorganisms. This study investigated the effects of supernatant metabolites from 29 halophilic bacterial strains on the stem cell-like properties of six human cancer cell lines. The metabolites from Salinivenus iranica demonstrated the most potent cytotoxic effect on breast cancer cell lines, with no effect on normal cells. The purified cytotoxic molecule, a phenol amine, downregulated the expression of the pluripotency gene SOX-2 in breast cancer cells.
SCIENTIFIC REPORTS
(2023)
Article
Ecology
Carlotta Meriggi, Maliheh Mehrshad, Richard K. Johnson, Ane T. Laugen, Stina Drakare
Summary: Species distribution models calibrated with bioclimatic variables suggest a high probability of range expansion of the invasive toxin producing cyanobacterium Raphidiopsis raciborskii to Sweden. However, field-based surveys and in-silico screening did not fully support this prediction, indicating the need for proactive monitoring with high temporal and spatial frequency. The inconsistencies between the models and monitoring approaches highlight the uncertainties in solely focusing on climate drivers and the potential limitations of early invasion detection.
ISME COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Yeganeh Farrokhi, Zeinab Neshati, Parastoo Saniee, Ali Makhdoumi
Summary: This study found that two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, have the ability to form biofilm and combat H. pylori biofilm formation. Compared to reference probiotics, Lactobacillus casei and Bifidobacterium bifidum, Bacillus and Enterococcus strains showed higher biofilm rates. They were also more effective in inhibiting H. pylori biofilm formation on polystyrene and AGS cells. Bacillus and Enterococcus strains achieved inhibition by promoting the expression of luxS and decreasing the expression of ropD.
INTERNATIONAL MICROBIOLOGY
(2023)
