Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 510, Issue 1, Pages 42-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2016.06.017
Keywords
Doxorubicin; Cellular drug delivery; Shear stress; Biomimetic dynamic conditions; Liposomal formulation; Cell-killing efficiency; Cell microenvironment
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Funding
- Ministry of Science, ICT 300 and Future Planning, Patient centric RD Project [2013M3A9B5075841]
- National Research Foundation (Korea-China Cooperation Project), Korea
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Shear stress could be considered in the context of cellular uptake and cell-killing efficiency. Thus, mimicking the dynamic characteristics of in vivo environment is important in targeted drug delivery. We investigated the intracellular uptake and cell-killing efficiency of doxorubicin (DOX) in a free and liposomal form (Doxil (R)) under biomimetic shear stress to mimic in vivo environment. In this dynamic environment, cells demonstrated significantly higher fluorescence intensity than that of the static environment, and fluorescence microscopy images indicated increased intracellular uptake of DOX in the presence of fluidic shear stress. In cells treated with free DOX and liposomal Doxil (R), cell-killing efficiency was affected by shear stress. Taken together, shear stress, affecting drug uptake and cell-killing efficiency, is important in intracellular drug targeting. (C) 2016 Elsevier B.V. All rights reserved.
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