Review
Environmental Sciences
Sajjad Hussain, Ping Ouyang, Yingkun Zhu, Abdul Khalique, Changliang He, Xiaoxia Liang, Gang Shu, Lizi Yin
Summary: The failure of traditional vaccination against Salmonella necessitates the development of novel therapeutics. Understanding the role of T3SS in bacterial pathogenesis and the development of anti-virulence agents is crucial, and the inhibition of T3SS at different stages of infection can help in controlling the disease progression. Insights into T3SS inhibitors of Salmonella can mitigate antibiotic resistance and lead to the development of new therapeutics.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Immunology
Duc-Cuong Bui, Tian Luo, Jere W. McBride
Summary: Bacteria in the Rickettsiales order, which are transmitted by arthropod vectors, cause life-threatening infections in humans and animals. The Type 1 Secretion System (T1SS) and associated effectors play important roles in evading host immune responses and promoting intracellular survival. This review summarizes current knowledge on the roles of T1SS effectors in Rickettsiales infections and explores newly identified immunoreactive proteins as potential targets for a protective immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Zongchao Chen, Wenlong Han, Min Zhu, Dan Liu, Pengfei Niu, Ruyu Shen, Chan Ding, Shengqing Yu
Summary: Riemerella anatipestifer secretes proteins through the type IX secretion system (T9SS), and GldM and GldK are found to be crucial virulence factors. This study reveals that the gldG gene encodes the GldG protein, which plays a key role in bacterial virulence and protein secretion. The mutant strain Yb2 Delta gldG shows reduced gliding motility and deficient protein secretion.
VETERINARY MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Yonghui Wang, Meihui Hou, Zhaodong Kan, Guanghui Zhang, Yunxia Li, Lei Zhou, Changfa Wang
Summary: Three type III secretion system inhibitors were identified through virtual screening and biological evaluation, showing potential as lead molecules for anti-Salmonella drug development. These compounds exhibited comparable activity to the positive control gatifloxacin against Salmonella abortus equi strains.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Naama Wagner, Michael Alburquerque, Noa Ecker, Edo Dotan, Ben Zerah, Michelle Mendonca Pena, Neha Potnis, Tal Pupko
Summary: In this study, novel machine-learning algorithms were developed to characterize the secretion signal of Type III effectors in both plant and animal pathogens. A benchmark dataset was curated and the results showed that the novel approach had substantially better classification accuracy compared to previous methodologies. The study also revealed that plant and animal pathogen effectors may have different secretion signals.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Nicole C. Thunes, Rachel A. Conrad, Haitham H. Mohammed, Yongtao Zhu, Paul Barbier, Jason P. Evenhuis, David Perez-Pascual, Jean-Marc Ghigo, Ryan S. Lipscomb, John R. Schneider, Nan Li, Devon H. Erbes, Clayton Birkett, Benjamin R. LaFrentz, Timothy J. Welch, Mark J. McBride
Summary: Flavobacterium columnare, a pathogen that causes columnaris disease in freshwater fish, poses a major threat to sustainable aquaculture. Researchers have identified a genetically manipulable strain, MS-FC-4, which has facilitated the identification of virulence factors. Deletion of core genes eliminates virulence, and several secreted proteins have been identified as important for pathogenicity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biology
Moirangthem Kiran Singh, Parisa Zangoui, Yuki Yamanaka, Linda J. Kenney
Summary: Genetic code expansion was successfully used to site-specifically label previously intractable targets SsaP and SifA, demonstrating the superiority of this method for imaging purposes.
Review
Biochemistry & Molecular Biology
Dukas Jurenas, Laure Journet
Summary: The bacterial T6SS system secretes a variety of toxic effectors that can affect other bacteria or cells through different mechanisms. These effectors can disrupt the cell envelope, affect DNA integrity, cell division, or deplete energy resources, helping bacteria dominate their niche.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Megan Y. Nas, Jeffrey Gabell, Nicholas P. Cianciotto
Summary: Previously, it was found that Stenotrophomonas maltophilia possesses a T4SS that can kill other bacteria, with two effectors, RS14245 and RS14255, identified as key components in killing pathogenic Pseudomonas aeruginosa. These findings represent a significant advancement in understanding S. maltophilia, T4SS function, clinically relevant interactions, and bactericidal activities.
Article
Plant Sciences
Xiaoli Liu, Jun Cai, Xiaoxu Li, Feng Yu, Dousheng Wu
Summary: The article hypothesizes that type III effectors manipulate the microbiota composition by mediating ternary pathogen-plant-microbiota interactions, providing new insights for studying the biological functions of type III effectors and developing new strategies for plant disease control.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Microbiology
Brian C. Russo, Jeffrey K. Duncan-Lowey, Poyin Chen, Marcia B. Goldberg
Summary: This study investigates the role of actin polymerization in T3SS-mediated translocation, showing that it is essential for the assembly of translocon pore in an open conformation and effector translocation. The activation of T3SS involves a complex process where host signals are sensed by the translocon pore, leading to effector translocation.
Article
Fisheries
Youting Zhu, Xingkun Jin, Ziyan Fang, Xing Zhang, Dan Li, Weiwei Li, Qun Wang
Article
Fisheries
Zi-Yan Fang, Dan Li, Xue-Jie Li, Xing Zhang, You-Ting Zhu, Wei-Wei Li, Qun Wang
FISH & SHELLFISH IMMUNOLOGY
(2016)
Article
Cell Biology
Lucrecia Alberdi, Alexandra Vergnes, Jean-Baptiste Manneville, Dumizulu L. Tembo, Ziyan Fang, Yaya Zhao, Nina Schroeder, Audrey Dumont, Margaux Lagier, Patricia Bassereau, Lorena Redondo-Morata, Jean-Pierre Gorvel, Stephane Meresse
JOURNAL OF CELL SCIENCE
(2020)
Article
Cell Biology
Ziyan Fang, Mathieu Fallet, Thomas Moest, Jean-Pierre Gorvel, Stephane Meresse
Summary: In this study, it was found that the microtubule molecular motor KIF1B beta plays a key role in the establishment of the Salmonella replication niche, and its absence affects the stability of bacterial vacuoles and the formation of associated tubules. Kinesin-3 interacts with the Salmonella effector SifA and the host protein complex SKIP. The presence of SifA establishes a recruitment pathway for kinesin-1 and kinesin-3 in infected cells.
JOURNAL OF CELL SCIENCE
(2022)