Giant Oyster Mushroom Pleurotus giganteus (Agaricomycetes) Enhances Adipocyte Differentiation and Glucose Uptake via Activation of PPARy and Glucose Transporters 1 and 4 in 3T3-L1 Cells

The edible mushroom Pleurotus giganteus was tested for its effect on adipocyte differentiation and glucose uptake activity in 3T3-L I cells. The basidiocarps of P giganteus were soaked in methanol to obtain a crude methanol extract and then fractionated to obtain an ethyl acetate extract. In this study, cell proliferation was measured using an MTT assay, lipid accumulation using an Oil Red 0 assay, and glucose uptake using a fluorescence glucose uptake assay. Gene expression was measured via real-time polymerase chain reaction analysis with TagMan primer. Ethyl acetate extract significantly enhanced adipogenic differentiation and glucose uptake in 3T3-1,1 adipocytes via the expression of sterol regulatory element-binding protein, peroxisome proliferator-activated receptor gamma, and phosphatidylinositol 3-kinase/Akt. Glucose uptake was facilitated by the highly expressed glucose transporters Glutl and Glut4. Taken together, these results suggest that P. giganteus ethyl acetate extract has an insulin-sensitizing effect on adipocytes and has potential as an adjuvant for the management of type 2 diabetes.