Journal
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
Volume 13, Issue 4, Pages 292-297Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijms.14187
Keywords
HSCR; LncRNA; Molecular diagnosis
Categories
Funding
- Natural Science Foundation of China [NSFC 81370473, NSFC 81400574, NSFC 81570467]
- Natural Science Foundation of Jiangsu Province of China [BK20131388]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Background: Long non-coding RNAs (lncRNAs) have been reported to participate in various diseases. Hirschsprung disease (HSCR) is a common digestive disease in the new born. However, the relationship between lncRNAs and HSCR remains unclarified. Methods: We used qRT-PCR to detect the relative expression of LOC101926975 in 80 pairs of HSCR bowel tissues and matched normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were then used to evaluate the function in vitro by knocking down the LOC101926975 in SK-N-BE(2) cells. Receiver operating characteristic (ROC) curve was used to evaluate the potential diagnostic value of LOC101926975. Results: LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1. Dysregulation of LOC101926975 suppressed cell proliferation and induced G0/G1 arrest without impact on cell apoptosis or migration. Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value. Conclusions: Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.
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