Article
Biotechnology & Applied Microbiology
Yi-Xuan Wang, Zhu-Li Peng, Zhi-Wen Sun, Yan-Jun Pan, Hong Ai, Zhi-Hui Mai
Summary: This study investigates the role of miR-20 in promoting osteogenic differentiation in BMSCs and accelerating bone formation in a rabbit maxillary sinus bone defect model. Overexpression of miR-20a increased the levels of osteogenic markers and enhanced calcified nodule deposition in BMSCs, while anti-miR-20a treatment had the opposite effect. In the rabbit model, miR-20a delivery significantly improved bone density, osteoblast numbers, and collagen type 1 secretion compared to Bio-Oss bone powder.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Chih-Yi Yang, Rita Jui-Hsien Lu, Ming-Kang Lee, Felix Shih-Hsian Hsiao, Ya-Ping Yen, Chun-Chun Cheng, Pu-Sheng Hsu, Yi-Tzang Tsai, Shih-Kuo Chen, I-Hsuan Liu, Pao-Yang Chen, Shau-Ping Lin
Summary: MSCs lacking DNMT3L exhibit reduced colony forming ability and impaired osteogenesis in vitro, with differentially regulated genes associated with bone morphology-related phenotypes. The observed defects are hypothesized to result from pre-deposited epigenetic signatures from DNMT3L-expressing progenitors, rather than a direct effect of missing DNMT3L in MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Sarah A. Schoonraad, Alan A. Jaimes, Arjun J. X. Singh, Kiera J. Croland, Stephanie J. Bryant
Summary: This study investigates the osteogenic effects of tethered BMP-2 and BMP-9 on human MSCs encapsulated in a hydrogel. The results show that tethered BMP-2 and BMP-9 both effectively enhance osteogenesis of MSCs, leading to a mature osteoblast phenotype.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Hyun-Jin Lee, Sae-Kyung Min, Yoon-Hee Park, Jun-Beom Park
Summary: The study demonstrates that BMP-7 enhances alkaline phosphatase activity and mineralization of stem cells cultured on deproteinized bovine bone mineral, with higher effects observed at a concentration of 100 ng/mL. Furthermore, BMP-7 is associated with increased expression of RUNX2 and its connection to the mitogen-activated protein kinase pathway.
Article
Immunology
Chiara Tomasoni, Corinne Arsuffi, Samantha Donsante, Alessandro Corsi, Mara Riminucci, Andrea Biondi, Alice Pievani, Marta Serafini
Summary: Acute myeloid leukemia (AML) cells have a negative impact on the osteogenic differentiation of normal bone marrow stromal cells (BMSCs), and direct interaction between AML cells and BMSCs plays a vital role in this process. AML-mediated activation of Notch signaling is involved in the ineffective osteogenic commitment of BMSCs, resulting in the formation of a pre-osteoblast-rich niche supportive of leukemia development in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Cheng Su, Xiao Zheng, Yanjin He, Li Long, Wenchuan Chen
Summary: This study identified aberrant expression of circRNAs in hUCMSCs during osteogenic differentiation, constructed competing endogenous RNA networks using bioinformatics tools, elucidated underlying mechanisms, and validated the osteogenic-promoting effect of a specific circRNA.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Feng Wang, Wenbo Wang, Lingchi Kong, Li Shi, Mengwei Wang, Yimin Chai, Jia Xu, Qinglin Kang
Summary: This study found that ADM2 accelerates bone regeneration by enhancing the osteogenic differentiation and pro-angiogenic potential of BMSCs, possibly through the NF-κB/β-catenin and AKT/β-catenin pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Junjie Shen, Yi Sun, Xuanzhe Liu, Yu Zhu, Bingbo Bao, Tao Gao, Yimin Chai, Jia Xu, Xianyou Zheng
Summary: EGFL6 was found to stimulate angiogenesis and osteogenic differentiation through the Wnt/beta-catenin signaling pathway in vitro, and promoted CD31(hi)EMCN(hi) type H-positive capillary formation in a rat tibia distraction osteogenesis model. It enhanced the osteogenic differentiation potential of BMSCs and accelerated bone regeneration by stimulating angiogenesis.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Feng Wang, Huijuan Qian, Lingchi Kong, Wenbo Wang, Xiaoyu Wang, Ze Xu, Yimin Chai, Jia Xu, Qinglin Kang
Summary: Astragaloside IV (AS-IV) accelerates bone regeneration during distraction osteogenesis by enhancing osteogenesis and preosteoclast-induced angiogenesis simultaneously, partially through AKT/GSK-3 beta/beta-catenin signaling. These findings suggest that AS-IV may serve as a potential bioactive molecule for promoting the coupling of osteogenesis and angiogenesis, and imply that AKT/GSK-3 beta/beta-catenin signaling may be a promising therapeutic target for patients during distraction osteogenesis treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biophysics
Zhibiao Bai, Kai Hu, Zeyu Shou, Han Zhou, Jiahuan Yu, Hongming Meng, Liangyan Chen, Tiantian Yu, Ruofei Lu, Na Li, Chun Chen
Summary: This study investigated the effect of PC/Mucin multilayers on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). The results showed that PC/Mucin multilayers promoted the differentiation of BMSCs into osteoblasts and this effect was mediated by the activation of the Wnt/β-catenin pathway. These findings have important implications for the development of materials and coatings for bone regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Pharmacology & Pharmacy
Jinwu Bai, Jianxiang Xu, Kai Hang, Zhihui Kuang, Li Ying, Chenwei Zhou, Licheng Ni, Yibo Wang, Deting Xue
Summary: This study found that glycyrrhizic acid (GA) promotes osteogenic differentiation and enhances calcium deposition by modulating the Wnt/β-catenin signaling pathway. Experimental results demonstrated that GA-GelMA hydrogels accelerate bone healing, showing potential as a cost-effective treatment for bone defects.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Engineering, Biomedical
Meng Li, Jing Wu, Wenbo Geng, Yulu Yang, Xuan Li, Kun Xu, Ke Li, Yan Li, Qiaojian Duan, Pengfei Gao, Kaiyong Cai
Summary: Localized corrosion of 316L stainless steel reduces the viability, adhesion, proliferation, and osteogenic differentiation abilities of BMSCs. It causes cytotoxicity through the release of metallic ions and generates reactive oxygen species (ROS) around the corrosion pits, leading to oxidative stress and cell membrane depolarization. This study provides insights into corrosion-mediated degradation of cell function and material-cell interactions.
Article
Biotechnology & Applied Microbiology
Mi Eun Kim, Jong Keun Seon, Ju Yeon Kang, Taek Rim Yoon, Jun Sik Lee, Hyung Keun Kim
Summary: BFP-4 induces stronger osteogenic differentiation of BMSCs compared to BMP-7, as well as enhancing ALP activity, calcium concentration, and osteogenic factor production. This suggests that BFP-4-stimulated cell therapy may be a more efficient and cost-effective approach to bone regeneration and formation than BMP-7-based therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Cell Biology
Jun Li, Ting Zhang, Chenglong Huang, Min Xu, Wenhua Xie, Qilin Pei, Xinxin Xie, Bin Wang, Xi Li
Summary: The study revealed the role of chemerin in bone metabolism, demonstrating its ability to promote osteogenic differentiation and bone formation. This provides a new theoretical basis for chemerin as a therapeutic strategy for osteoporosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)