Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.

Automated summary

Cellular heterogeneity is important in cell-based assays, and electrical impedance sensing technology allows for rapid, non-invasive acquisition of single cell electrical parameters. These parameters are closely related to cellular properties and activities, and devices such as impedance flow cytometry and electrical impedance spectroscopy are used for cell recognition, counting, viability detection, phenotypic assay, and other cell detection. Impedance sensing technology shows promise in single-cell analysis.