Article
Biochemistry & Molecular Biology
Prayag J. Amin, Bhavani S. Shankar
Summary: This study assessed the effect of Tinospora cordifolia polysaccharide G1-4A on natural killer (NK) cells. It was found that G1-4A can directly activate NK cells and also activate them through interactions with dendritic cells (DC). This research suggests that G1-4A has the potential to be used as an immunotherapeutic agent.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Review
Immunology
Nurul Izza Ismail
Summary: As the placenta is semi-allogenic, it is expected that maternal immune cells would attack the invading trophoblast, but studies have shown that they cooperate with the trophoblast to disrupt the arterial wall. uNK cells, unique immune cells, play a role in pregnancy. The interaction between HLA class 1b genes and maternal uNK cells in humans is described in this review.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Shance Li, Chaoting Zhang, Luyan Shen, Xia Teng, Yefei Xiao, Bentong Yu, Zheming Lu
Summary: NK cells can be engineered for TCR therapy in tumor treatment. A chimeric TCR NK cell was designed and showed antigen-specific recognition and cytotoxicity. This approach has potential clinical applications.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Review
Immunology
Xin Chen, Lei Jiang, Xuesong Liu
Summary: This review discusses the contributions of natural killer (NK) cells in cancer treatment, explores the current strategies at harnessing NK cells for cancer treatment, and highlights the importance of NK cell-mediated therapies in the next wave of cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Sara Martin-Iglesias, Lara Herrera, Silvia Santos, Miguel angel Vesga, Cristina Eguizabal, Senentxu Lanceros-Mendez, Unai Silvan
Summary: Natural killer (NK) cells are lymphocytes that play a crucial role in eliminating tumor and virus-infected cells. Their activation is based on direct interaction with target cells through inhibitory and activating receptors. This article critically examines the steps involved in isolating, expanding, and storing NK cells, as well as their response to these manipulations. The manipulation of NK cells is important for improving the effectiveness and applicability of NK cell-based therapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Giorgio Santoni, Consuelo Amantini, Matteo Santoni, Federica Maggi, Maria Beatrice Morelli, Angela Santoni
Summary: NK cells play a crucial role in immune protection against viruses and tumors by killing target cells and secreting cytokines. Mechanotransduction, mediated by actin cytoskeleton, regulates NK cell functions such as adhesion, migration, and tissue infiltration. Further research on mechanotransduction may lead to new immunotherapeutic approaches tailored for NK cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Ye Zhang, Wei Kuang, Danfeng Li, Yu Li, Yi Feng, Xinwei Lyu, Gao-Bo Huang, Jian-Qi Lian, Xiao-Fei Yang, Cheng Hu, Yajuan Xie, Song Xue, Jiali Tan
Summary: NKB cells play a crucial role in periodontitis by secreting IL-18 to promote inflammation and bone resorption. Elevated levels of NKB cells and IL-18 were observed in periodontitis patients and correlated with attachment loss. Additionally, IL-18 stimulation led to proinflammatory cytokine production in periodontal ligament cells, suggesting a potential target for immunotherapies in periodontitis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
An-Liang Guo, Yan-Mei Jiao, Qi-Wen Zhao, Hui-Huang Huang, Jian-Ning Deng, Chao Zhang, Xing Fan, Ruo-Nan Xu, Ji-Yuan Zhang, Cheng Zhen, Zhi-Man Xie, Ying-Mei Qin, Jian-Qing Xu, Yu Yang, Ming Shi, Lei Huang, Jin-Wen Song, Fu-Sheng Wang
Summary: This study discovered the presence of CXCR5(+) NK cells in the lymph nodes of HIV-1 infected individuals, which were inversely related to the viral burden of HIV-1. These cells exhibited anti-HIV-1 characteristics and may serve as a potential target for curing HIV-1.
Article
Immunology
Bailin He, Qiusui Mai, Yunyi Pang, Shikai Deng, Yi He, Rongtao Xue, Na Xu, Hongsheng Zhou, Xiaoli Liu, Li Xuan, Chengyao Li, Qifa Liu
Summary: CD19 CAR engineered memory-like NK cells exhibit enhanced persistence and antitumor activity against CD19(+) tumors, providing a potential approach to treat patients with relapsed or refractory B cell malignancies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Zhenglin Ou, Yebin Lu, Dayong Xu, Zhen Luo
Summary: This study reveals that low oxygen conditions can decrease the expression of miR-1275 and negatively affect NK cell functionality. The miR-1275/AXIN2 axis plays a crucial role in immune escape in pancreatic cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Ana Vuletic, Katarina Mirjacic Martinovic, Nevena Tisma Miletic, Jerome Zoidakis, Sergi Castellvi-Bel, Milena Cavic
Summary: The crosstalk between immune cells and tumor cells in the TME influences EMT, with NK cells playing a significant role in controlling metastasis in CRC. However, the suppressive TME can negatively impact NK cell function through modulation of receptor and ligand expression. Additionally, EMT in CRC is associated with increased expression of immune checkpoint molecules on functionally exhausted NK cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Johanna Bodder, Tasmin Zahan, Rianne van Slooten, Gerty Schreibelt, I. Jolanda M. de Vries, Georgina Florez-Grau
Summary: Immunotherapeutic approaches have revolutionized the treatment of diseases like cancer, with a focus on modulating anti-tumor immune responses. Natural killer (NK) cells and dendritic cells (DCs) play crucial roles in cancer immunotherapy, and their bidirectional cross-talk within the tumor microenvironment is essential for treatment success.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Siqin Duan, Shuwen Liu
Summary: This review highlights the immunotherapy strategies for HIV infection that utilize the beneficial properties of natural killer (NK) cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Songbo Zhao, Jiazhi Duan, Yalin Lou, Ruyun Gao, Shanshan Yang, Piming Wang, Chunhua Wang, Lin Han, Minghuan Li, Chunhong Ma, Xiaohong Liang, Hong Liu, Yuanhua Sang, Lifen Gao
Summary: A novel immunotherapy approach utilizing NK cells conjugated with magnetic nanoparticles has been developed for treating solid tumors, demonstrating improved tumor targeting and inhibition capabilities through magnetic field guidance.
Review
Oncology
Azadeh Rahimi, Zahra Malakoutikhah, Ilnaz Rahimmanesh, Gordon A. Ferns, Reza Nedaeinia, Sayed Mohammad Matin Ishaghi, Nasim Dana, Shaghayegh Haghjooy Javanmard
Summary: This translation describes the importance of melanoma cell metastasis in patients and the role of NK cells in the immune system in combating melanoma and inhibiting metastasis. It also emphasizes the complex relationship between the melanoma tumor microenvironment and NK cells.
CANCER CELL INTERNATIONAL
(2023)
Letter
Biochemistry & Molecular Biology
Larissa Lipskaia, Pauline Maisonnasse, Charles Fouillade, Valentin Sencio, Quentin Pascal, Jean-Michel Flaman, Emmanuelle Born, Arturo London-Vallejo, Roger Le Grand, David Bernard, Francois Trottein, Serge Adnot
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jonathan L. Torres, Gabriel Ozorowski, Emanuele Andreano, Hejun Liu, Jeffrey Copps, Giulia Piccini, Lorena Donnici, Matteo Conti, Cyril Planchais, Delphine Planas, Noemi Manganaro, Elisa Pantano, Ida Paciello, Piero Pileri, Timothee Bruel, Emanuele Montomoli, Hugo Mouquet, Olivier Schwartz, Claudia Sala, Raffaele De Francesco, Ian A. Wilson, Rino Rappuoli, Andrew B. Ward
Summary: As the COVID-19 pandemic continues, highly potent monoclonal antibodies are needed to combat SARS-CoV-2 variants of concern. This study evaluates the efficacy of mAb J08 against these variants and investigates the binding details using cryo-EM and X-ray crystallography. The results demonstrate that mAb J08 has strong affinity against most variants and binds at a specific location on the receptor binding domain, away from the mutation sites. These findings further support the use of mAb J08 as a monoclonal therapy in ongoing clinical trials.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Luis M. Molinos-Albert, Valerie Lorin, Valerie Monceaux, Sylvie Orr, Asma Essat, Jeremy Dufloo, Olivier Schwartz, Christine Rouzioux, Laurence Meyer, Laurent Hocqueloux, Asier Saez-Cirion, Hugo Mouquet
Summary: This study characterized the humoral immune response to HIV-1 in post-treatment controllers and found that they can be divided into two distinct groups based on viral behavior and immune signatures. Virally-exposed post-treatment controllers displayed stronger immune responses, which may contribute to their ability to control infection.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jeremy Dufloo, Cyril Planchais, Stephane Fremont, Valerie Lorin, Florence Guivel-Benhassine, Karl Stefic, Nicoletta Casartelli, Arnaud Echard, Philippe Roingeard, Hugo Mouquet, Olivier Schwartz, Timothee Bruel
Summary: Researchers have found that broadly neutralizing antibodies (bNAbs) can not only neutralize HIV-1, but also block the release of viral particles by trapping them on the surface of infected cells. This study reveals an additional antiviral mechanism of bNAbs.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Maxime Beretta, Hugo Mouquet
Summary: This article summarizes recent findings on the humoral immune response to HBV and explores the potential of human HBV neutralizing monoclonal antibodies as immunotherapeutics, providing new opportunities for achieving a functional cure for HBV.
CURRENT OPINION IN VIROLOGY
(2022)
Article
Immunology
Benjamin Vesin, Pierre Authie, Catherine Blanc, Ingrid Fert, Amandine Noirat, Fabien Le Chevalier, Yu Wei, Min-Wen Ku, Kirill Nemirov, Francois Anna, David Hardy, Cyril Planchais, Hugo Mouquet, Francoise Guinet, Pierre Charneau, Laleh Majlessi, Maryline Bourgine
Summary: In the face of SARS-CoV-2 variants and the limited efficacy of current vaccines, the development of more effective vaccines is necessary. Through intranasal administration, a viral vector encoding a stabilized Spike protein provides full-lung protection and reduces pulmonary inflammation against both the original and Omicron variant of SARS-CoV-2 in animal models. Furthermore, boosting with a viral vector encoding the Spike protein of the Omicron variant enhances cross-neutralization in previously primed animals. These findings shed light on vaccine effectiveness and support the use of intranasal vaccination.
Article
Multidisciplinary Sciences
Delphine Planas, Timothee Bruel, Isabelle Staropoli, Florence Guivel-Benhassine, Francoise Porrot, Piet Maes, Ludivine Grzelak, Matthieu Prot, Said Mougari, Cyril Planchais, Julien Puech, Madelina Saliba, Riwan Sahraoui, Florent Femy, Nathalie Morel, Jeremy Dufloo, Rafael Sanjuan, Hugo Mouquet, Emmanuel Andre, Laurent Hocqueloux, Etienne Simon-Loriere, David Veyer, Thierry Prazuck, Helene Pere, Olivier Schwartz
Summary: Convergent evolution of SARS-CoV-2 Omicron BA.2, BA.4, and BA.5 lineages have led to the emergence of several new subvariants, including BA.2.75.2, BA.4.6, and BQ.1.1. These subvariants carry additional mutations in the spike protein, potentially increasing transmissibility and evading immune responses. The study found that the efficacy of monoclonal antibodies and serum from vaccinated individuals against these subvariants varies.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Nell Saunders, Ignacio Fernandez, Cyril Planchais, Vincent Michel, Maaran Michael Rajah, Eduard Baquero Salazar, Jeanne Postal, Francoise Porrot, Florence Guivel-Benhassine, Catherine Blanc, Gaelle Chauveau-Le Friec, Augustin Martin, Ludivine Grzelak, Rischa Maya Oktavia, Annalisa Meola, Olivia Ahouzi, Hunter Hoover-Watson, Matthieu Prot, Deborah Delaune, Marion Cornelissen, Martin Deijs, Veronique Meriaux, Hugo Mouquet, Etienne Simon-Loriere, Lia van der Hoek, Pierre Lafaye, Felix Rey, Julian Buchrieser, Olivier Schwartz
Summary: In this study, it was found that TMPRSS2 is a functional receptor for coronavirus HKU1, triggering cell-cell fusion and viral entry. Designed anti-TMPRSS2 nanobodies were shown to inhibit HKU1 spike attachment and infection.
Article
Immunology
Nicolas Huot, Cyril Planchais, Pierre Rosenbaum, Vanessa Contreras, Beatrice Jacquelin, Caroline Petitdemange, Marie Lazzerini, Emma Beaumont, Aurelio Orta-Resendiz, Felix A. Rey, R. Keith Reeves, Roger Le Grand, Hugo Mouquet, Michaela Mueller-Trutwin
Summary: The study reveals that interferon-gamma (IFN-gamma) regulates the persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in bronchoalveolar macrophages from cynomolgus macaques up to 18 months postinfection. There is an interplay between NK cells and macrophages, and the production of IFN-gamma plays a regulatory role in the persistence of SARS-CoV-2.
Article
Multidisciplinary Sciences
Karunasinee Suphaphiphat, Delphine Desjardins, Valerie Lorin, Nastasia Dimant, Kawthar Bouchemal, Laetitia Bossevot, Maxence Galpin-Lebreau, Nathalie Dereuddre-Bosquet, Hugo Mouquet, Roger Le Grand, Mariangela Cavarelli
Summary: This study demonstrates that mucosal delivery of the bNAb 10-1074 in a microbicide gel can effectively inhibit cell-associated transmission of HIV-1 in non-human primates. The findings suggest that this approach has potential as a preventive strategy against infection mediated by infected cells.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Camille Chauvin, Laurine Levillayer, Mathilde Roumier, Hubert Nielly, Claude Roth, Anupama Karnam, Srinivasa Reddy Bonam, Anne Bourgarit, Clement Dubost, Aurore Bousquet, Sebastien Le Burel, Raphaele Mestiri, Daminen Sene, Joris Galland, Marc Vasse, Matthieu Groh, Mathilde Le Marchand, Camille Vassord-Dang, Jean-Francois Gautier, Pham-Thi Nhan, Christian Verny, Bruno Pitaro, Cyril Planchais, Hugo Mouquet, Richard Paul, Etienne Simon-Loriere, Jagadeesh Bayry, Laurent Gilardin, Anavaj Sakuntabhai
Summary: Although tocilizumab treatment has shown efficacy in severe COVID-19 patients, its short-term use does not affect B cell sub-populations and cross-neutralization of SARS-CoV-2 variants in convalescent COVID-19 patients. The study found that SARS-CoV-2-specific IgG1 titers were dependent on disease severity rather than tocilizumab treatment. Both treated and non-treated patients had strong neutralizing activity against ancestral and several variants of SARS-CoV-2, but weaker activity against the Gamma and Omicron variants. Overall, tocilizumab therapy did not modify the robustness of cell and IgG responses to Spike antigens.
Review
Immunology
Philippe Rascle, Griffin Woolley, Stephanie Jost, Cordelia Manickam, R. Keith Reeves
Summary: Natural killer (NK) cells play a critical role in defending against viral infections and cancers. The balance between activating and inhibitory NK cell receptors determines the overall NK cell response. NK cell education, which is influenced by factors such as self-major histocompatibility complex class I (MHC-I) molecules and inhibitory KIR expression, is crucial in generating tuned effector NK cell responses in different diseases. This review discusses the relationship between NK cell education and various physiological factors, as well as the diverse effector functions of educated NK cells in different pathologies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Aurelio Orta-Resendiz, Caroline Petitdemange, Sandrine Schmutz, Beatrice Jacquelin, Sophie Novault, Nicolas Huot, Michaela Muller-Trutwin
Summary: In this article, we present a protocol for setting three spectral flow cytometry panels for the characterization of human unconventional CD8+NKG2A/C+ T cells as well as other T and natural killer cell subsets. The protocol includes steps for standardizing, preparing, and staining the cells, as well as the experimental setup and final data analysis. This protocol has various applications, including immunophenotyping of limited samples, immune function evaluation/monitoring, and research in oncology, autoimmune, and infectious diseases.
