Article
Biochemistry & Molecular Biology
Guangliang Liu, Jie Chen, Xiaofang Wang, Yujiao Liu, Yufei Ma, Xiaolin Tu
Summary: Appropriate concentrations of growth factors, such as Wnt3a, were used to pretreat a 3D-bioprinted GelMA/PCL scaffold loaded with ST2 cells, promoting cell proliferation, osteogenic differentiation, and mineralization in vitro as well as enhancing angiogenesis and osteogenesis in vivo. Inhibition of Wnt/beta-catenin signaling reduced the bone repair effect of the scaffold, indicating the importance of growth factor pretreatment in enhancing bone repair biomaterials' activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Xiaocheng Wang, Yunru Yu, Chaoyu Yang, Changmin Shao, Keqing Shi, Luoran Shang, Fangfu Ye, Yuanjin Zhao
Summary: A versatile microfluidic 3D printing strategy was proposed to fabricate fibrous scaffolds with photothermal responsive channels to improve vascularization and bone regeneration. The thermal channeled scaffolds displayed reversible behavior controlled by near-infrared irradiation, facilitating cell penetration and prevascularization, while embedded BP nanosheets promoted in situ biomineralization and cell proliferation. These near-infrared responsive channeled scaffolds showed promising potential for tissue/vascular ingrowth in tissue engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Engineering, Biomedical
Vincent Fitzpatrick, Zaira Martin-Moldes, Anna Deck, Ruben Torres-Sanchez, Anne Valat, Dana Cairns, Chunmei Li, David L. Kaplan
Summary: The study aimed to use functionalized 3D-printed scaffolds for bone regeneration, incorporating silk-hydroxyapatite bone cements and various growth factors. Functionalities were assessed through cellular differentiation, migration, and proliferation, identifying synergistic effects among the growth factors.
Article
Engineering, Biomedical
Jinkyu Lee, Seung Jae Huh, Ji Min Seok, Sangmin Lee, Hayeon Byun, Gyu Nam Jang, Eunhyung Kim, Se-jeong Kim, Su A. Park, Sung Min Kim, Heungsoo Shin
Summary: In this study, we developed 3D printed scaffolds with bioactive surfaces coated with minerals and platelet-derived growth factors. The results showed that the mineral deposition and compressive modulus on the scaffold surface were enhanced, and the immobilization of PDGF further promoted osteogenic differentiation and the secretion of pro-angiogenic factors. In vivo experiments on mice demonstrated that the scaffold significantly improved bone regeneration and neovessel formation.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Multidisciplinary
Chao Dong, Hao Wei, Xiaonan Zhang, Yuxiao Li, Lifei Huang, Qingde Wa, Yongxiang Luo
Summary: Researchers have developed a core-shell fiber scaffold to promote bone repair. The scaffold provides adaptive structural cues and microenvironment for bone formation at different stages. The results show that the scaffold has excellent bone repair capacity, promoting new bone formation and blood vessel growth.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Engineering, Environmental
Yongxiang Luo, Tao Zhang, Xin Lin
Summary: The study describes a method to fabricate hydrogel scaffolds with designed macro pores and fully interconnected microchannel (FIM) networks using 3D printing and surface crosslinking. The FIM scaffold shows improved mechanical properties and the ability for fast perfusion in microchannels, demonstrating strong potential for tissue engineering applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Xiaocheng Wang, Yunru Yu, Chaoyu Yang, Luoran Shang, Yuanjin Zhao, Xian Shen
Summary: In this study, a responsive MX-HF scaffold is proposed to improve skin flap survival by promoting vascularization and skin flap regeneration. The scaffold demonstrates near-infrared responsiveness through the photothermal conversion capacity of MXene nanosheets and the temperature-responsive ability of poly(NIPAM) hydrogels, facilitating cell penetration into scaffold channels. Additionally, controlled delivery of vascular endothelial growth factor (VEGF) in the scaffold enhances angiogenesis.
Review
Engineering, Biomedical
Hongjian Zhang, Chengtie Wu
Summary: Neurovascular networks play important roles in tissue and organ regeneration. Traditional scaffolds cannot meet the requirement of angiogenesis and innervation, but 3D printing provides a versatile technique to fabricate biomimetic scaffolds. This review summarizes the progress in using 3D-printed biomaterials for vascularized and innervated tissue regeneration.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Chen Qin, Hongjian Zhang, Lei Chen, Meng Zhang, Jingge Ma, Hui Zhuang, Zhiguang Huan, Yin Xiao, Chengtie Wu
Summary: To achieve regeneration of highly vascularized and innervated tissues, a pre-angiogenic cell-laden scaffold with durable angiogenic functions is prepared. The scaffold supports long-term cell survival and growth, and exhibits durable angiogenic capability both in vitro and in vivo. The pre-angiogenic scaffolds can induce the neurogenetic differentiation and osteogenic differentiation, and may represent the future direction of biomaterials for complex tissue/organ regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Bo Yuan, Pin Liu, Rui Zhao, Xiao Yang, Zhanwen Xiao, Kai Zhang, Xiangdong Zhu, Xingdong Zhang
Summary: In this study, a silicon-doped nano-hydroxyapatite (nSiHA)/titanium dioxide (TiO2) composite coating with a hierarchical micro/nano-network structure is constructed on the surface of a 3D-printed porous Ti scaffold. The functionalized Ti scaffold not only has excellent osteoinduction ability but can also immobilize and release vascular endothelial growth factor (VEGF), promoting osteogenesis and angiogenesis. The functionalized Ti scaffold shows great potential in bone tissue regeneration and is a promising candidate for load-bearing bone defect repair.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Nazanin Owji, Nandin Mandakhbayar, Jae-Ryung Cha, Andrew R. Padalhin, Zalike Keskin Erdogan, Alaa Aldaadaa, Taleen Shakouri, Prasad Sawadkar, Oliver Frost, Hae-Won Kim, Elena Garcia-Gareta, Jonathan C. Knowles
Summary: At a time when health challenges are unpredictable, there is a high demand for functional implants, especially bone grafts. Bone tissue engineering substitutes have emerged as an alternative to conventional bone grafts. However, current approaches in this field have limitations that hinder their translation into clinical settings. This research aims to investigate the role of calcium phosphate incorporation in a mechanically stable, biologically functional, and 3D printable polymer for the reconstruction of complex craniofacial defects. The study found that such polymeric systems hold promise for designing 3D printed scaffolds for the reconstruction of maxillofacial defects.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Multidisciplinary
Xiaodi Wu, Yingying Huo, Zheng Ci, Yahui Wang, Wei Xu, Baoshuai Bai, Junxiang Hao, Guanhuai Hu, Mengyuan Yu, Wenjie Ren, Yixin Zhang, Yujie Hua, Guangdong Zhou
Summary: The study developed a novel bone-biomimetic porous hydrogel scaffold, which promoted VTEB regeneration and bone defect repair by combining osteogenic components and angiogenic substances.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Multidisciplinary
Hongjian Zhang, Meng Zhang, Dong Zhai, Chen Qin, Yufeng Wang, Jingge Ma, Hui Zhuang, Zhe Shi, Liang Wang, Chengtie Wu
Summary: Inspired by space-filling polyhedra, polyhedron-like scaffolds with spatial topologies are prepared via 3D-printing technology, and they significantly promote osteogenesis, angiogenesis, and neurogenesis in vitro, as well as inducing innervated and vascularized bone regeneration in vivo.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Lei Yang, Lu Fan, Xiang Lin, Yunru Yu, Yuanjin Zhao
Summary: A pearl powder hybrid bioactive scaffold developed by mimicking bone tissue extracellular matrix shows promising potential for bone regeneration. With the help of microfluidic-assisted 3D printing technology, the scaffold composition and structure can be accurately controlled. The scaffold, made of fish gelatin and pearl powder, exhibits good biocompatibility, cell adhesion, and osteogenic differentiation ability. Furthermore, the controlled release of vascular endothelial growth factor (VEGF) from the scaffold promotes angiogenesis, resulting in accelerated bone regeneration.
Article
Polymer Science
Roni Cohen, Ester-Sapir Baruch, Itai Cabilly, Assaf Shapira, Tal Dvir
Summary: Appropriate vascularization is crucial for the survival and function of tissues. Researchers have developed a modified ECM bioink to print large blood vessels with capillary beds using induced pluripotent stem cells derived endothelial cells (iPSCs-ECs).
Article
Engineering, Biomedical
Xin Sun, Jin Yang, Jie Ma, Tianchang Wang, Xue Zhao, Dan Zhu, Wenjie Jin, Kai Zhang, Xuzhou Sun, Yuling Shen, Neng Xie, Fei Yang, Xiushuai Shang, Shuai Li, Xiaojun Zhou, Chuanglong He, Deteng Zhang, Jinwu Wang
Summary: In this study, a modified gelatin-dopamine composite bioink was developed by grafting dopamine onto the molecular chain of gelatin, and it was used for constructing an artificial periosteum through 3D bioprinting. The results showed that the developed bioink exhibited good thermosensitivity and printability, and could be used to fabricate a 3D bioprinted artificial periosteum with high cell viability and adhesion. Additionally, the 3D bioprinted artificial periosteum effectively promoted osteogenesis both in vitro and in vivo. Therefore, the developed 3D bioprinted artificial periosteum holds great promise for bone defect repair.
Article
Materials Science, Biomaterials
Jin Yang, Zhihui Li, Shikai Li, Qianqian Zhang, Xiaojun Zhou, Chuanglong He
Summary: Three-dimensional bioprinting is a powerful technique for studying cell behavior and tissue properties. However, the limited diversity of bioinks is a major challenge in 3D extrusion bioprinting. In this study, a natural biopolymer-based formula with dual crosslinking performance was developed to formulate a cell-laden bioink.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Multidisciplinary
Tongtong Shi, Hanzhi Lu, Jianyong Zhu, Xiaojun Zhou, Chuanglong He, Fulun Li, Guang Yang
Summary: A multifunctional hydrogel dressing composed of keratin, protocatechuic aldehyde (PA) and iron ions was successfully fabricated, which exhibited injectability, self-healing ability, tissue adhesion, antibacterial properties and anti-inflammation effects. The incorporation of phellopterin (PP) further enhanced the anti-inflammatory properties of the hydrogel. In experiments with diabetic mice, the PP-loaded hydrogel significantly shortened inflammation duration, promoted macrophage polarization towards anti-inflammatory phenotype, and accelerated wound repair.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Qianqian Zhang, Xiaojun Zhou, Haibo Du, Yujie Ha, Yao Xu, Rongguang Ao, Chuanglong He
Summary: A dual-drug delivery scaffold system combining a 3D printed scaffold with hydrogel was fabricated for the treatment of infectious bone defects. The scaffold incorporated biodegradable mesoporous silica nanoparticles and vancomycin-loaded hydrogel to provide structural support, promote angiogenesis and osteogenesis, and control infection. In vitro and in vivo experiments showed that the composite scaffold had antimicrobial properties, excellent biocompatibility, and promoted bone regeneration. This bifunctional scaffold has potential application in the treatment of infected bone defects.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Yuan Dai, Weizhong Wang, Xiaojun Zhou, Linli li, Yuyi Tang, Minghao Shao, Feizhou Lyu
Summary: The transplantation of human neural stem cells (hNSCs) combined with bioactive nanofibrous scaffold can promote neural regeneration, reduce inflammation, and restore spinal cord function in rats. This study provides insights into potential treatment strategies for spinal cord injury using NSCs and bioactive biomaterials.
ACS APPLIED NANO MATERIALS
(2023)
Article
Engineering, Biomedical
Xiaojun Zhou, Zunjuan Wang, Tao Li, Zhonglong Liu, Xin Sun, Weizhong Wang, Liang Chen, Chuanglong He
Summary: In this study, a polyelectrolytes modified-biomimetic scaffold with macroporous and nanofibrous structures was successfully prepared for efficient bone defect healing. The scaffold incorporated strontium-substituted hydroxyapatite (SrHA) and exhibited sequential release of BMP-2 protein and Sr ions. The polyelectrolytes modification improved the scaffold's hydrophilicity and protein binding efficiency, promoting cell proliferation and tissue infiltration. Additionally, the dual-factor loaded scaffold enhanced bone regeneration through spatiotemporal delivery of BMP-2 and Sr ions, as evidenced by increased vascularization and new bone formation. This biomimetic scaffold as a dual-factor delivery system shows great potential for bone regeneration applications.
MATERIALS TODAY BIO
(2023)
Article
Microbiology
Shana Chen, Quan Fu, Mandlaa Mandlaa, Qingchun Wang, De Sheng, Saijilahu Saijilahu, Tana Tana, Dezhi Yang
Summary: Brucellosis is rapidly spreading in Inner Mongolia, China, and investigating its genetics may provide insights into bacterial host adaptation mechanisms. This study reports the genome sequence of Brucella melitensis strain BM6144 isolated from a human patient.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Review
Biochemistry & Molecular Biology
Shuo Chen, Lijuan Wang, Lei Yang, Abdus Samad Rana, Chuanglong He
Summary: Organoid is an emerging frontier technology in the field of life science, wherein pluripotent stem cells or tissue-derived differentiated/progenitor cells form 3D structures according to their multi-directional differentiation potential and self-assembly ability. This review summarizes the recent development of engineered biomimetic microenvironments for organoids, including the composition of the matrix for organoid culture and strategies for engineering the microenvironment from biophysical, biochemical, and cellular perspectives. The newly developed monitoring technologies are also reviewed, and a brief conclusion and outlook for future research are presented.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Nanoscience & Nanotechnology
Qian Feng, Xiaojun Zhou, Chuanglong He
Summary: This review provides a comprehensive overview of the application of near-infrared (NIR) light in bone tissue engineering, including the introduction of NIR probes and responsive materials, visualization of bone regeneration, and treatment of bone-related diseases. It also discusses the existing challenges and future development directions of NIR light-based bone tissue engineering.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ziyi Lu, Shan Du, Jiaxun Li, Min Zhang, Huali Nie, Xiaojun Zhou, Fulun Li, Xinwei Wei, Jinqiang Wang, Fuyao Liu, Chuanglong He, Guang Yang, Zhen Gu
Summary: The Langmuir-Blodgett technique is used to deposit antibacterial nanoparticles onto microneedles, providing a fast-acting and long-lasting antibacterial effect without sacrificing their payload capacity, drug release, or mechanical strength.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Ai Yang, Yue Wang, Qian Feng, Kanwal Fatima, Qianqian Zhang, Xiaojun Zhou, Chuanglong He
Summary: This study fabricated a multifunctional scaffold based on a hard-and-soft integration strategy, which can simultaneously promote osteogenesis and allow for non-invasive monitoring of in vivo bone regeneration. The scaffold significantly enhances bone formation through controlled release of simvastatin (SV) and enables visualization of scaffold degradation using alkaline phosphatase-responsive near-infrared II fluorescence imaging and magnetic resonance imaging.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Jianqiu Yang, Zhenzhen Huang, Jiang Tan, Jingye Pan, Shixuan Chen, Wenbing Wan
Summary: By functionalizing a decellularized pomelo peel with an adhesive hydrogel and antibacterial materials, the hybrid wound dressing can effectively inhibit bacterial infection, promote granulation tissue formation and angiogenesis, and reduce scar formation during wound healing.
BIOACTIVE MATERIALS
(2024)
Article
Engineering, Biomedical
Yong Mei, Xiaohua Qin, Zhenyu Yang, Shiyao Song, Xiaoting Liu, Chong Wu, Jieying Qian, Xiaowan Huang, Yunjiao Zhang, Weiling He
Summary: This article presents a novel nanoparticle for treating KRAS-TP53 co-mutation in gastrointestinal cancers. The nanoparticle can degrade mutant p53 proteins (mutp53) and deliver the drug AMG510 to inhibit mutant KRAS and mutp53 signaling pathways. The results show that the nanoparticle can effectively reduce cell proliferation and migration, and demonstrate remarkable therapeutic efficacy in a tumor-bearing mouse model.
BIOACTIVE MATERIALS
(2024)
