Journal
BIOACTIVE MATERIALS
Volume 8, Issue -, Pages 296-308Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.06.030
Keywords
In vivo printing; Handheld bioprinter; Adhesive scaffolds; GelMA; VEGF; Wound healing
Funding
- National Institutes of Health [GM126831, AR073822]
- Stepping Strong Innovator Award
Ask authors/readers for more resources
This study demonstrates the benefits of in vivo printed adhesive scaffolds for the treatment of full-thickness wounds. The scaffold is able to adhere to curved surfaces of wet tissues and release growth factors to enhance wound healing. The introduced in vivo printing strategy is convenient and does not require expensive equipment.
Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues, without the need for any sutures. The scaffold is further shown to offer a sustained release of VEGF, enhancing the migration of endothelial cells in vitro. Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds. The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place, such as an operating room, and does not require expensive bioprinters or imaging modalities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available