Article
Ophthalmology
Mimi Liu, Han Wen, Lei Lin, Chaoxiang Ge, Lili Guo, James Chodosh, Qinxiang Zheng, Wei Chen
Summary: This retrospective case series investigated the short-term visual outcomes and complications of a modified Boston Type-II keratoprosthesis (BK2) procedure using autologous auricular cartilage reinforcement. The study found that the modified AACR-BK2 procedure could be considered to restore vision in patients with end-stage corneal blindness.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Materials Science, Biomaterials
Silvia Gonzalez Gomez, Jordi Guillem-Marti, Helena Martin-Gomez, Carlos Mas-Moruno, Maria-Pau Ginebra, Francisco Javier Gil, Rafael Ignasi Barraquer, Jose Maria Manero
Summary: This study aimed to improve the performance of the rough topography used in commercial BKPro by using a dual peptide platform. The findings showed that adding a functionalized coating on the rough surface of titanium samples could enhance cell proliferation, reduce the risk of fibrosis, and exhibit good antibacterial activity.
BIOMATERIALS ADVANCES
(2023)
Article
Ophthalmology
Soumaya Bouhout, Marie-Claude Robert, Mona Harissi-Dagher
Summary: Reimplantation of a second B-KPro is a potentially sight- and globe-saving procedure for eyes with B-KPro failure, but the prognosis is guarded with risks of complications such as corneal melt, retroprosthetic membrane, and retinal detachment. Ambulatory vision can be salvaged in a third of patients, while another third may progress to loss of light perception.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Pukhraj Rishi, Ekta Rishi, Pradeep Manchegowda, Geetha Iyer, Bhaskar Srinivasan, Shweta Agarwal
Summary: This study analyzed the incidence, microbiological profile, risk factors, and outcomes in eyes with osteo-odonto keratoprosthesis (OOKP) developing endophthalmitis. The results showed that endophthalmitis had a significant impact on OOKP eyes, with Steven-Johnson syndrome and laminar resorption being the most important risk factors.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Engineering, Biomedical
Venkata Avadhanam, Ganesh Ingavle, Yishan Zheng, Sandeep Kumar, Christopher Liu, Susan Sandeman
Summary: A hydrogel polymer composite was developed as a synthetic substitute for the OOKP skirt, mimicking the alveo-dental lamina by incorporating nano-crystalline hydroxyapatite (nHAp) coated PLGA microspheres. The IPN hydrogel combinations supported cell adhesion and proliferation, while also showing non-inflammatory potential, making them promising materials for OOKP skirt substitutes.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Ophthalmology
Diana Khair, Roy Daoud, Mona Harissi-Dagher
Summary: The purpose of this study was to evaluate the incidence, rate of formation, and risk factors of retroprosthetic membrane (RPM) after Boston type 1 keratoprosthesis (BKPro) and describe its treatment. The study found that more than one third of patients who undergo BKPro will develop RPM, which is strongly correlated with retinal detachment. Simultaneous intraocular lens extraction is protective against RPM, while simultaneous lens extraction significantly increases the rate of RPM formation. Nd:YAG membranectomy is a commonly used treatment approach.
Article
Ophthalmology
Jonathan El-Khoury, Majd Mustafa, Roy Daoud, Mona Harissi-Dagher
Summary: The study evaluated the time needed for patients with Boston type 1 keratoprosthesis (KPro) to reach their best-corrected visual acuity (BCVA) and identified factors that might affect this time duration. The majority of subjects reached their BCVA between 3 and 6 months after KPro implantation, with postoperative glaucoma, retroprosthetic membrane (RPM) and endophthalmitis prolonging this duration.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Review
Ophthalmology
Steven Bonneau, C. Maya Tong, Yelin Yang, Mona Harissi-Dagher
Summary: This review compares the outcomes of PKP and BKPro in high-risk patients, and suggests that BKPro may provide better treatment outcomes than PKP in most cases. However, further research is needed to validate these conclusions.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Taylor Nayman, Cristina Bostan, Andrei-Alexandru Szigiato, Mona Harissi-Dagher
Summary: A study was conducted comparing the long-term outcomes of primary and secondary KPro implantation, showing that primary KPro resulted in better visual outcomes but had higher complication rates and lower retention rates than secondary KPro. This difference is likely due to preoperative indications.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Sina Sharifi, Hannah Sharifi, Curtis Guild, Mohammad Mirazul Islam, Khoa D. Tran, Corrina Patzer, Claes H. Dohlman, Eleftherios Paschalis, Miguel Gonzalez-Andrades, James Chodosh
Summary: E-beam irradiation enhances the mechanical properties of human donor corneas and maintains stability, showing no detrimental effects on corneal tissues and BK-cornea devices.
Article
Ophthalmology
Li-Qiang Wang, Teng-Yun Wu, Xiao-Niao Chen, Ze-Quan Xu, Min Yang, Ran Xiang, Xiao Ma, Shan Zhang, Yi-Fei Huang
Summary: The study reported long-term outcomes of Boston keratoprosthesis type I (B-KPro type I) in treating severe ocular surface disorders, showing it as a viable option especially for patients with chemical and thermal burns. The visual outcomes and retention rate were found to be improved and maintained in most cases.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Swapna S. Shanbhag, Sirisha Senthil, Ashik Mohamed, Sayan Basu
Summary: This study analyzed the outcomes of keratoprosthesis in eyes with limbal stem cell deficiency and compared two different devices. Results showed that Boston Kpro had slightly better anatomical retention and functional recovery compared to AuroKpro, but there was no significant difference in complication rates between the two groups.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2021)
Review
Neurosciences
Claes Dohlman
Summary: Millions of people worldwide are bilaterally blind due to corneal diseases, corneal transplantation can restore sight but the success rate decreases in eyes with chronic inflammation and corneal vascularization, and donor cornea material may be limited. Development of artificial cornea is the only option for some patients with eye diseases not suitable for transplantation.
ANNUAL REVIEW OF VISION SCIENCE
(2022)
Review
Ophthalmology
Gustavo Ortiz-Morales, Denise Loya-Garcia, Maria F. Colorado-Zavala, Daniela E. Gomez-Elizondo, Matias Soifer, Bhaskar Srinivasan, Shweta Agarwal, Alejandro Rodriguez-Garcia, Victor L. Perez, Guillermo Amescua, Geetha Iyer
Summary: This article provides an analysis of the modified osteo-odonto-keratoprosthesis (MOOKP) using a comprehensive search of published case reports and series. The study examines various factors including demographic profile, indications for surgery, surgical techniques, postoperative management, outcomes, and complications. The findings demonstrate that MOOKP is a reliable option for visual rehabilitation in patients with bilateral corneal blindness and end-stage ocular surface disease, with high long-term success rates in both anatomical and functional aspects.
Article
Materials Science, Biomaterials
Qing Li, Haibin Zhao, Hongmei Wang, Guoqun Zhao
Summary: This study aims to develop an ideal scaffold for Boston Keratoprosthesis type I (B-KPro) using acellular porcine cornea (APC) crosslinked with ultraviolet A (UVA)/riboflavin. Through comprehensive analysis, the optimal crosslinking condition was determined and successful transplantation experiments were conducted in rabbit cornea models.
BIOMATERIALS ADVANCES
(2022)
Article
Cell & Tissue Engineering
Kieran Joyce, Zaklina Buljovcic, Goran Rosic, Marietta Kaszkin-Bettag, Abhay Pandit
Summary: This article evaluates the regulatory landscape of tissue-engineered products (TEPs) and identifies the trends in clinical trial activity in the European Union (EU). The study highlights the most active member states, commercial involvement, a shift toward the use of allogeneic cells, and a renewed interest in TEP development.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Neurosciences
Vaibhav Patil, Raghvendra Bohara, Carla Winter, Michelle Kilcoyne, Siobhan McMahon, Abhay Pandit
Summary: This study investigated the glycosylation modulation in a mixed glial culture model and identified considerable differences in glycosylation from acute to chronic phases. Clustering analysis revealed dense clusters for control and LPS treated groups, while the cytokine combination group was scattered into different clusters. Lectin immunostaining confirmed the presence of various glycans in astrocytes and microglia, including GalNAc, GlcNAc, mannose, fucose, and sialic acid-binding residues. Inhibition of sialyltransferase suppressed the upregulation of sialic acid expression, resulting in modulation of mitochondrial functions.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Yazhong Bu, Wenwen Zhang, Sergio Martin-Saldana, Amir M. Alsharabasy, Mark Da Costa, Luyao Feng, Ze Zhang, Xinlan Ge, Chonghui Li, Shichun Lu, Abhay Pandit
Summary: This study develops a series of multifunctional bioadhesives using succinic anhydride-modified hyaluronic acid (HASA) and plant-derived tannic acid (TA) with centrifuging and freeze-drying. By varying the ratio of HASA to TA, HASATA 1:2, 1:7, and 1:14 are fabricated with tunable adhesive strength. The bioadhesives accelerate wound healing, exhibit antioxidant properties, and have antibacterial activity, making them suitable for noninvasive wound closure in the clinical setting.
ADVANCED MATERIALS INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Sean O'Halloran, Abhay Pandit, Andreas Heise, Andrew Kellett
Summary: Two-photon polymerization (TPP) is a leading method for microscale fabrication of customized polymeric devices and surfaces. It has a wide range of applications in optics, drug delivery, tissue engineering, and microfluidics. TPP has evolved from standard acrylate- and epoxy-based photoresists to custom formulated monomers that can modify the properties of resulting structures. This article provides an overview of the essential principles and advanced chemical modification strategies for TPP materials and introduces commonly used photoresists and their applications.
Article
Nanoscience & Nanotechnology
Cristian Salvador, Patrizia Andreozzi, Gabriela Romero, Iraida Loinaz, Damien Dupin, Sergio E. Moya
Summary: Small interference RNA (siRNA) is a gene modulation tool that can silence genes associated with genetic disorders. However, the instability of siRNA in the blood and its difficulty in reaching the cytosol limit its therapeutic potential. Polyamine-based nanoparticles, particularly polyallylamine hydrochloride (PAH), show promise in gene delivery. In this study, PAH was chemically modified with oleic acid to improve cell viability and siRNA transfection efficiency. The PAH-oleic acid substitutes were effective in silencing the CD47 protein, making them a potential candidate for immunotherapy.
ACS APPLIED BIO MATERIALS
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Robert Frederickson, Thomas Ritter, Kevin V. Morris
Review
Ophthalmology
Arianne J. H. Van Velthoven, Tor P. Utheim, Maria Notara, Dominique Bremond-Gignac, Francisco C. Figueiredo, Heli Skottman, Daniel Aberdam, Julie T. Daniels, Giulio Ferrari, Christina Grupcheva, Carina Koppen, Mohit Parekh, Thomas Ritter, Vito Romano, Stefano Ferrari, Claus Cursiefen, Neil Lagali, Vanessa L. S. Lapointe, Mor M. Dickman
Summary: Congenital aniridia is a panocular disorder characterized by iris hypoplasia and aniridia-associated keratopathy (AAK), leading to loss of vision. The understanding of the molecular pathogenesis of AAK may provide new insights for its management, as there is currently no approved therapy to delay or prevent its progression. This review discusses the biological mechanisms involved in AAK development and explores potential future treatment options including surgical, pharmacological, cell therapies, and gene therapies.
SURVEY OF OPHTHALMOLOGY
(2023)
Article
Multidisciplinary Sciences
Paolo Contessotto, Renza Spelat, Federico Ferro, Vaidas Vysockas, Ausra Krivickiene, Chunsheng Jin, Sandrine Chantepie, Clizia Chinello, Audrys G. Pauza, Camilla Valente, Mindaugas Rackauskas, Alvise Casara, Vilma Zigmantaite, Fulvio Magni, Dulce Papy-Garcia, Niclas G. Karlsson, Egle Ereminiene, Abhay Pandit, Mark Da Costa
Summary: The lack of a reproducible pre-clinical model hinders the study of the pathophysiology and possible interventions for non-ST-segment elevation myocardial infarction (NSTEMI). In this study, authors develop an ovine model to reproduce post-ischemic remodeling in NSTEMI and reveal distinct complex sugar moieties in cellular membranes and extracellular matrix patterns in infarcted tissue.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Hannah Egan, Oliver Treacy, Kevin Lynch, Niamh A. Leonard, Grace O'Malley, Eileen Reidy, Aoise O'Neill, Shania M. Corry, Kim De Veirman, Karin Vanderkerken, Laurence J. Egan, Thomas Ritter, Aisling M. Hogan, Keara Redmond, Li Peng, Jenny Che, Wayne Gatlin, Pushpa Jayaraman, Margaret Sheehan, Aoife Canney, Sean O. Hynes, Emma M. Kerr, Philip D. Dunne, Michael E. O'Dwyer, Aideen E. Ryan
Summary: Immunosuppressive tumor microenvironments (TMEs) in cancer reduce immune responses, and mesenchymal stromal cells (MSCs) promote tumor progression through enhancing immune cell suppression in colorectal cancer (CRC). The hyper-sialylation of glycans promotes immune evasion by binding sialic acids to Siglecs receptors on immune cells, resulting in inhibition of their functions. However, the role of sialylation in MSC/CAF immunosuppression in the TME is not well characterized.
Review
Biotechnology & Applied Microbiology
Aert F. Scheper, Jack Schofield, Raghvendra Bohara, Thomas Ritter, Abhay Pandit
Summary: Glycosylation is a process of modifying proteins and lipids with complex carbohydrates called glycans. It is not template-driven like genetic transcription or protein translation, but rather dynamically regulated by metabolic flux. This review provides an overview of the metabolic pathways involved in glycan synthesis and explains the dysregulation of glycosylation in inflammation. The resulting inflammatory hyperglycosylation acts as a glycosignature of disease, and studies on metabolic inhibitors targeting critical enzymes have identified potential glycotherapeutic approaches to inflammation.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Pharmacology & Pharmacy
Vaibhav Patil, Raghvendra Bohara, Vijaya Krishna Kanala, Siobhan Mcmahon, Abhay Pandit
Summary: Spinal cord injury leads to chronic inflammation and glial scar formation caused by the activation of microglia and astrocytes. Current anti-inflammatory strategies have limitations and do not consider the progression of glial cell-derived inflammation. Understanding this progression is crucial for evaluating therapeutic strategies. New models can be used as high-throughput screening platforms for novel therapeutics.
DRUG DISCOVERY TODAY
(2023)
Meeting Abstract
Cell & Tissue Engineering
Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Abhay Pandit, Aleksandra Klimczak
TISSUE ENGINEERING PART A
(2023)
Article
Nanoscience & Nanotechnology
Cristian Salvador, Patrizia Andreozzi, Gabriela Romero, Iraida Loinaz, Damien Dupin, Sergio E. Moya
Summary: Small interference RNA (siRNA) is a gene modulation tool that can silence genes related to genetic disorders. Polyamine-based nanoparticles, particularly polyallylamine hydrochloride (PAH) covalently functionalized with oleic acid, show promise in gene delivery and siRNA transfection. The substitution of PAH with oleic acid improves cell viability and siRNA transfection efficiency, particularly in silencing the CD47 protein for potential use in immunotherapy.
ACS APPLIED BIO MATERIALS
(2023)