Review
Endocrinology & Metabolism
Ziru Li, Clifford J. Rosen
Summary: Bone marrow adipose tissue (BMAT) is a significant component of the bone marrow space in healthy adults, but its expansion is associated with aging, obesity, anorexia nervosa, and irradiation. While traditionally viewed as a negative component, recent studies have revealed that BMAT can serve as an energy reservoir and also plays a role in regulating bone formation and hematopoiesis. This review summarizes the unique characteristics of BMAT and provides an updated understanding of its physiological roles based on a newly established mouse model.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biology
Zengdi Zhang, Zan Huang, Mohamed Awad, Mohammed Elsalanty, James Cray, Lauren E. Ball, Jason C. Maynard, Alma L. Burlingame, Hu Zeng, Kim C. Mansky, Hai-Bin Ruan
Summary: In mammals, the interaction between bone marrow stroma and hematopoietic progenitors contributes to bone-BM homeostasis, but the mechanisms and interactions between the development of skeletal and hematopoietic systems are largely unknown. This study reveals that the intracellular O-GlcNAc modification plays a role in dictating the fate and function of early BM stromal cells (BMSCs). O-GlcNAcylation promotes osteogenic differentiation of BMSCs and supports lymphopoiesis, while inhibiting adipogenesis and myelopoiesis. Impaired bone formation and defective hematopoiesis were observed when O-GlcNAc transferase (OGT) was ablated in BMSCs.
Article
Multidisciplinary Sciences
Jianfang Wang, Qiaoling Zhu, Dandan Cao, Qiqi Peng, Xiaoying Zhang, Chong Li, Chen Zhang, Bo O. Zhou, Rui Yue
Summary: This study identifies bone marrow stromal cells (BMSCs) and megakaryocytes/platelets (MKs/PLTs) as two important sources of IGF-1 that coordinate to maintain and regenerate the adult skeleton, highlighting reciprocal regulation between the hematopoietic and skeletal systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Oncology
Amanpreet Kaur Bains, Lena Behrens Wu, Jennifer Riviere, Sandra Rother, Valentina Magno, Jens Friedrichs, Carsten Werner, Martin Bornhaeuser, Katharina S. Goetze, Michael Cross, Uwe Platzbecker, Manja Wobus
Summary: Myelodysplastic syndromes (MDS) are a heterogeneous group of blood malignancies with specific features and a high risk of progression to acute myeloid leukemia. This study found that MSC-derived extracellular matrix (ECM) plays an important role in MDS, with structural alterations observed in MDS ECM and a potential involvement of glycosaminoglycans (GAGs) in this process.
FRONTIERS IN ONCOLOGY
(2022)
Review
Immunology
Alice Pievani, Roberto Savoldelli, Juliane Poelchen, Elisa Mattioli, Giorgio Anselmi, Alice Girardot, Jochen Utikal, Pierre Bourdely, Marta Serafini, Pierre Guermonprez
Summary: Tissue engineering provides numerous opportunities in regenerative medicine, drug testing, and modeling hematopoiesis. Replicating the physiological microenvironments supporting leukocyte development is crucial for accurately modeling immune cell development. Immune niches are characterized by mesenchymal stromal cells that play unique roles in organizing niche development, maintenance, and function.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Charlotte Hellmich, Edyta E. Wojtowicz
Summary: Normal bone marrow homeostasis relies on a steady supply of nutrients, and changes in metabolite demands can occur in response to infection and immune stress. The right balance of dietary components helps maintain normal homeostasis and cope with systemic stress. Some dietary components can also lead to chronic inflammation, impacting immune function. Optimal nutrition supports immune cell functions to effectively combat pathogens and resolve responses, while avoiding chronic inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Pascal Fichtel, Malte von Bonin, Robert Kuhnert, Kristin Moebus, Martin Bornhaeuser, Manja Wobus
Summary: Aging of the hematopoietic system is characterized by an expansion of hematopoietic stem and progenitor cells (HSPCs) with reduced capacity for engraftment, self-renewal, and lymphoid differentiation, resulting in myeloid-biased hematopoiesis.
The communication between HSPCs and MSCs is mediated by direct cell-cell contacts or extracellular vesicles (EVs) that carry bioactive substances. Aging MSCs secrete fewer miRNAs, but aged MSC-EVs contain higher levels of specific miRNAs such as miR-29a and miR-34a. Young EVs enhance the cell number and viability of HSPCs, while aged MSC-EVs promote the differentiation of HSPCs into erythroid and erythroid-megakaryocytic progenitor cells. The therapeutic relevance of MSC-derived EVs in modulating HSPC characteristics needs further investigation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Ziru Li, Ormond A. MacDougald
Summary: Laboratory mice are a valuable preclinical model for studying common human diseases related to osteopenia or hematopoietic disorders. However, factors such as species, age, sex, skeletal site, and treatment protocol should all be taken into consideration when choosing the appropriate model for future studies.
BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Fulvio Massaro, Florent Corrillon, Basile Stamatopoulos, Nathalie Meuleman, Laurence Lagneaux, Dominique Bron
Summary: The aging process of bone marrow and the alteration of mesenchymal stromal cells' functions play a crucial role in hematopoiesis and tumor progression. Understanding the mechanisms of MSC aging is essential for comprehending hematologic malignancies and developing therapeutic strategies. The dynamic interactions between hematopoietic stem cells and their microenvironment in bone marrow highlight the importance of studying MSC senescence in cancer progression.
Article
Engineering, Biomedical
Meng Li, Jing Wu, Wenbo Geng, Yulu Yang, Xuan Li, Kun Xu, Ke Li, Yan Li, Qiaojian Duan, Pengfei Gao, Kaiyong Cai
Summary: Localized corrosion of 316L stainless steel reduces the viability, adhesion, proliferation, and osteogenic differentiation abilities of BMSCs. It causes cytotoxicity through the release of metallic ions and generates reactive oxygen species (ROS) around the corrosion pits, leading to oxidative stress and cell membrane depolarization. This study provides insights into corrosion-mediated degradation of cell function and material-cell interactions.
Article
Cell Biology
Mary Mohrin, Justin Liu, Jose Zavala-Solorio, Sakshi Bhargava, John Maxwell Trumble, Alyssa Brito, Dorothy Hu, Daniel Brooks, Georgios Koukos, Lama Alabdulaaly, Jonathan S. Paw, Kayley Hake, Ganesh Kolumam, Mary L. Bouxsein, Roland Baron, Yuliya Kutskova, Adam Freund
Summary: PAPP-A deficiency modulates bone marrow homeostasis by regulating the number and activity of mesenchymal stromal cells (MSCs), which are not only the major source of extracellular matrix (ECM) but also play essential roles in the development of adult bone, regulation of marrow adiposity, and formation of the hematopoietic niche. This mechanism may contribute to the extended longevity and healthspan associated with PAPP-A deletion.
Review
Clinical Neurology
Zili Wang, Xudong Wang, Yidong Liao, Guangtang Chen, Kaya Xu
Summary: Stroke is a major cause of death and disability worldwide, and current treatment options are limited. Bone marrow mesenchymal stromal cells (BMSCs) offer a new approach for stroke treatment, but research on the precise inflammatory mechanism is lacking. In this review, we summarize the immune response of BMSCs in stroke treatment and provide a new perspective for stem cell therapy.
FRONTIERS IN NEUROLOGY
(2022)
Article
Engineering, Biomedical
Shuming Sun, Han Liu, Yan Hu, Yanpeng Wang, Mingri Zhao, Yijun Yuan, Yafei Han, Yingying Jing, Jin Cui, Xiaoxiang Ren, Xiao Chen, Jiacan Su
Summary: In this study, human skeletal muscle-specific ssDNA aptamers were selected and identified using SELEX. The aptamers showed preferential interaction with human skeletal muscle cells in vitro and specifically targeted these cells in vivo. The formation of a disulfide bond with nanoliposomes did not affect the ability of the aptamers to target skeletal muscle cells. This newly identified ssDNA aptamer and nanoliposome modification have potential applications in the treatment of human skeletal muscle diseases.
BIOACTIVE MATERIALS
(2023)
Review
Cell Biology
Yuyuan Gu, Yan Hu, Hao Zhang, Sicheng Wang, Ke Xu, Jiacan Su
Summary: This review summarizes the pathogenesis of osteoarthritis and the applications of single-cell RNA sequencing. By describing the microstructural changes in articular cartilage, meniscus, synovium and subchondral bone, it explores the interactions among various cells during disease progression. Additionally, it focuses on the potential applications of single-cell RNA sequencing in targeted drugs and tissue engineering, and reviews the current status of evaluation of bone-related biomaterials. Furthermore, the article discusses the potential combination of single-cell multi-omics technologies and personalized therapy for osteoarthritis.
CELL PROLIFERATION
(2023)
Article
Cell Biology
Jiadong Li, Wencai Zhang, Xinru Liu, Guangfeng Li, Yuyuan Gu, Kun Zhang, Fuming Shen, Xiang Wu, Yingying Jiang, Qin Zhang, Fengjin Zhou, Ke Xu, Jiacan Su
Summary: The balance between subchondral angiogenesis and articular damage in osteoarthritis (OA) remains unknown. The lack of specific drugs limits clinical treatment options, leading to joint destruction. Stat3 activation in subchondral bone H-type vessels induces endothelial cell proliferation and angiogenesis in OA. Inhibition of Stat3 activation alleviates angiogenesis-mediated bone deterioration and cartilage loss, suggesting Stat3 blockade as a promising therapeutic regimen for OA.
CELL PROLIFERATION
(2023)
Review
Engineering, Environmental
Han Liu, Jinru Sun, Mingkai Wang, Sicheng Wang, Jiacan Su, Can Xu
Summary: Inflammatory bowel disease (IBD) is a serious gastrointestinal disease characterized by pain, diarrhea, and bloody stools. Intestinal organoids and organoids extracellular vesicles (OEVs) have shown great potential in IBD treatment. Intestinal organoids have various applications in disease modeling, drug development, and host-microbe interactions, while OEVs have physiological effects and can participate in physiological and pathological processes. This review provides an overview of IBD and its current treatments, as well as the construction and applications of intestinal organoids and OEVs in IBD treatment. The advantages and challenges of these approaches are also discussed.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Multidisciplinary
Xiaochen Sun, Yi Zhang, Jin Cui, Chenxi Zhang, Chunlei Xing, Huihui Bian, Juan Lv, Dagui Chen, Lan Xiao, Jiacan Su, Yuanyuan Liu, Li Su
Summary: In this study, Gs and SF were used to improve burn wound healing by controlling inflammation and promoting fibroblast migration and proliferation. The multilayer composite dressing design achieved sustained release of Gs, inhibiting inflammatory response and promoting cell proliferation and collagen synthesis, resulting in improved wound healing.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Engineering, Multidisciplinary
Han Liu, Hao Zhang, Sicheng Wang, Jin Cui, Weizong Weng, Xinru Liu, Hua Tang, Yan Hu, Xiaoqun Li, Kun Zhang, Fengjin Zhou, Yingying Jing, Jiacan Su
Summary: Osteoporosis is a common bone degenerative disease with conventional treatments having side effects. This study successfully treated osteoporosis using modified probiotics carrying curative molecules, which improved bone structure with good biocompatibility and brought hope to patients.
COMPOSITES PART B-ENGINEERING
(2023)
Review
Pharmacology & Pharmacy
Dongyang Zhou, Fengjin Zhou, Shihao Sheng, Yan Wei, Xiao Chen, Jiacan Su
Summary: Osteoarthritis (OA) is characterized by progressive cartilage degeneration. Drug delivery via intra-articular administration (IA) can be restricted by various challenges. Nanodrug delivery systems, such as liposomes, micelles, and nanoparticles (NPs), have shown great potential to overcome these challenges and improve OA treatment.
DRUG DISCOVERY TODAY
(2023)
Review
Chemistry, Multidisciplinary
Mengmeng Li, Biao Yu, Sicheng Wang, Fengjin Zhou, Jin Cui, Jiacan Su
Summary: Nanomedicines with controlled drug release have gained attention for bone diseases. The design of nanocarriers based on bone microenvironment and pathological changes allows targeted and stimuli-responsive drug release. By exploiting the unique features of the bone pathological microenvironment, targeted and stimuli-responsive nanocarriers can be developed for effective drug enrichment.
Article
Engineering, Biomedical
Long Bai, Mengmeng Li, Jiacan Su
Summary: The design principles of traditional hydrogels hinder their effectiveness in guiding bone regeneration, as they do not consider the biomimetic regulation of multiple stages in bone healing. DNA hydrogels, based on synthetic biology, have the potential to overcome these limitations due to their resistance to enzymatic degradation, programmability, structural controllability, and mechanical properties. However, the 3D printing of DNA hydrogels is not well-defined and is still in its early stages.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Chemistry, Physical
Ruina Han, Yan Wu, Yafei Han, Xiangfei Liu, Han Liu, Jiacan Su
Summary: Autoimmune diseases are characterized by immune system dysregulation, and plant extracellular vesicles (PEVs) show promise in treating these diseases due to their ability to transport bioactive molecules and other advantageous properties. This review summarizes the characteristics, applications, and challenges of PEVs, and proposes engineering approaches and administration methods.
Review
Engineering, Biomedical
Yan Hu, Hao Zhang, Sicheng Wang, Liehu Cao, Fengjin Zhou, Yingying Jing, Jiacan Su
Summary: The necessity of disease models for bone/cartilage related disorders is well recognized, but the barrier between ex-vivo cell culture, animal models and the real human body has been unresolved for decades. The organoid-on-a-chip technique presents an opportunity to revolutionize basic research and drug screening for diseases like osteoporosis and arthritis. The bone/cartilage organoid on-chip (BCoC) system is a novel multi-tissue platform that faithfully emulates essential elements, biological functions, and pathophysiological responses. In this review, we propose the concept of the BCoC platform, summarize the basic modules and current efforts to integrate them on a single microfluidic system. Current disease models, unresolved problems, and future challenges are also discussed, with the aim of deeper understanding diseases and the ultimate realization of generic ex-vivo tools for further therapeutic strategies of pathological conditions.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Yili Wang, Qiushui Lin, Hao Zhang, Sicheng Wang, Jin Cui, Yan Hu, Jinlong Liu, Mengmeng Li, Kun Zhang, Fengjin Zhou, Yingying Jing, Zhen Geng, Jiacan Su
Summary: Diabetes mellitus predisposes to delayed fracture healing due to chronic inflammation. Macrophages polarization into M1 or M2 subtypes plays a crucial role in fracture healing with pro- or anti-inflammatory functions. M2-exosomes, with low immunogenicity and high bioactivity, were extracted in this study and used to intervene diabetic fracture healing. The results showed that M2-exosomes modulate the osteoimmune microenvironment by reducing M1 macrophages proportion, thus accelerating diabetic fracture healing. It was further confirmed that M2-exosomes induce M1 to M2 macrophage conversion through the PI3K/AKT pathway. This study provides a fresh perspective and potential therapeutic approach for M2-exosomes in improving diabetic fracture healing.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Shuyue Hao, Mingkai Wang, Zhifeng Yin, Yingying Jing, Long Bai, Jiacan Su
Summary: The treatment of large bone defects is a huge challenge in orthopedic and craniomaxillofacial surgery. Traditional strategies in bone tissue engineering have focused on mimicking the structure and composition of the bone extracellular matrix, while neglecting the synergistic effects of other cues from the microenvironment during bone regeneration. Microenvironment-targeted strategies are crucial for successful bone regeneration and offer promising solutions for advancing bone tissue engineering.
MATERIALS TODAY BIO
(2023)
Review
Materials Science, Biomaterials
Yutong Chen, Mengru Zhu, Biaotong Huang, Yingying Jiang, Jiacan Su
Summary: Due to the specific structure of natural bone, most therapeutics are unable to effectively reach the targeted site. Nanotechnology provides a solution by using cell membrane mimetic nanoparticles, which combine the advantages of natural cell membranes with synthetic nanoparticles to improve biocompatibility, circulation, and targeted delivery. This article provides an overview of the preparation and properties of cell membrane-coated nanoparticles, as well as their potential applications in bone therapy. The functionalized modification of cell membranes and the prospects and challenges of using cell membrane-coated nanoparticles in translational medicine are also discussed.
BIOMATERIALS ADVANCES
(2023)
Review
Materials Science, Biomaterials
Biaotong Huang, Zhifeng Yin, Fengjin Zhou, Jiacan Su
Summary: Bone tumors, both primary and metastatic, have poor prognosis. Functional biomaterial scaffolds are considered ideal for repairing defective bone tissue and preventing cancer recurrence. Various novel approaches, such as photodynamic and photothermal therapies, drug-conjugated therapies, and immune adjuvant-assisted therapies, have shown efficacy against tumors with low immunogenicity. This review summarizes the progress of research on biomaterial scaffolds in bone tumors and discusses the feasibility and advantages of combining multiple functionalization strategies. The potential obstacles to the clinical translation of anti-tumor bone bioscaffolds are also highlighted. This review provides valuable references for future advanced biomaterial scaffold design and clinical bone tumor therapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
