Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 226, Issue -, Pages 13-19Publisher
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2016.03.008
Keywords
Lactobacillus sanfranciscensis; Sourdough; Multilocus sequence typing; Pulsed-field gel electrophoresis
Categories
Funding
- Oniris
- ANR Bakery [13-ALID-0005-09]
Ask authors/readers for more resources
Lactobacillus sanfranciscensis is the predominant key lactic acid bacterium in traditionally fermented sourdoughs. Despite its prevalence, sourdough and their related breads could be different regarding their physicochemical and sensorial characteristics. The intraspecific diversity of L. sanfranciscensis might explain these observations. Fifty-nine strains isolated from French sourdoughs were typed by a polyphasic approach including Multilocus Sequence Typing (MIST) and Pulsed-field Gel Electrophoresis (PFGE), in order to study their genotypic diversity. MIST scheme can be reduced from six to four gene fragments (gdh, gyrA, nox and pta) without a major loss of discrimination between strains. The genes mapA and pgmA are not good candidates for inclusion in an MIST scheme to type L sanfranciscensis strains, as they could not be amplified for a set of 18 strains among the 59 studied. This method revealed 20 sequence types (STs). Of these, 19 STs were grouped in one clonal complex, showing a strong relatedness between these strains. PFGE using SmaI discriminated 41 pulsotypes and so distinguished isolates better than the MLST scheme. Both genotypic methods indicate a low diversity between strains isolated from the same sourdough and a higher diversity between strains isolated from different sourdoughs, suggesting an influence of baker practices and/or environmental conditions on the selection of strains. The use of these two methods targeting genetic variations gives an optimal genotypic characterization of L. sanfranciscensis strains. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available