Article
Chemistry, Analytical
Xia Xiao, Chenchen Li, Yuzhi Liu, Yaqian Feng, Kai Han, Haoyue Xiang, Guoyue Shi, Hui Gu
Summary: A ratiometric electrochemical microsensor (REM) was developed for in vivo measurement of cerebral Cl- by utilizing uniform Ag nanoparticles and methylene blue to achieve high sensitivity, selectivity, stability, reproducibility, and anti-biofouling.
Article
Chemistry, Analytical
Fan He, Yudong Shen, Juewen Liu
Summary: Melamine binding to polythymine (poly-T) DNA was studied using SYBR Green I staining and DNA melting experiments, revealing the formation of intramolecular complexes with multiple melamine molecules. A FRET sensor was designed for ratiometric detection with an apparent K-d of 85 μM and a limit of detection of 10.7 μM melamine.
Article
Biophysics
Syed Muhammad Usama, Donald R. Caldwell, Pradeep Shrestha, Michael P. Luciano, Nimit L. Patel, Joseph D. Kalen, Joseph Ivanic, Martin J. Schnermann
Summary: This study details the synthesis and evaluation of a class of norcyanine compounds that enable pH responsive fluorescence imaging in the long wavelength range by installing a hydrophilic substituted quaternary amine, showing promising photophysical properties.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Materials Science, Multidisciplinary
Vinita Bhardwaj, S. K. Ashok Kumar, Suban K. Sahoo
Summary: This study prepared red-emitting rod-type aggregates L and found that the restriction of intramolecular motion and the ESIPT phenomenon played a key role in emitting red fluorescence at 597 nm. The aggregates L showed red-emitting fluorescence between pH 5.4 and 9.1, green-emitting fluorescence above pH 9.9, and non-fluorescent behavior in acidic pH. L in HEPES buffer showed a selective turn-off response towards Cu2+ with a detection limit of 2.7 μM.
Article
Chemistry, Analytical
Junxiang Peng, Hongxia Chen, Mingtai Sun, Huan Yu, Jing Hou, Suhua Wang
Summary: The balance of acid-base levels is crucial for cellular functions and diseases prevention. A newly developed ratiometric fluorescent probe Ox-L based on 3H-indolium dye shows high sensitivity for detecting intracellular pH fluctuations, indicating great potential for biological applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xiao Liu, Xiangyang Gong, Jie Yuan, Xiaopeng Fan, Xingxing Zhang, Tianbing Ren, Sheng Yang, Ronghua Yang, Lin Yuan, Xiao-Bing Zhang
Summary: A new dual-stimulus responsive near-infrared reversible ATP-pH probe has been developed for fluorescence and photoacoustic ratiometric imaging of tumors, effectively reducing cumulative response after intravenous injection and suitable for in vivo imaging.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Hongxia Xu, Shankun Yao, Yuncong Chen, Changli Zhang, Shuren Zhang, Hao Yuan, Zhongyan Chen, Yang Bai, Tao Yang, Zijian Guo, Weijiang He
Summary: A novel ratiometric sensor, BDPS1, was developed to detect labile copper fluctuations in animals, showing an effective design rationale for NIR ratiometric sensors for copper tracking in vivo/ex vivo. The use of BDPS1 allowed for tracking of copper changes in mice and revealed distinct labile copper increases in different organs following injection of the therapeutic agent CUTX-101.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Applied
Murugan Thulasi Meenu, Anila Rose Cherian, Daisy Rajaian Sherin, Aiswarya Raveendran Nair, Thanathu Krishnan Manojkumar, Kokkuvayil Vasu Radhakrishnan, Anitha Varghese
Summary: Berberrubine (BBn) is a ratiometric naturally occurring fluorescent pH probe for the direct detection of diabetic ketoacidosis (DKA) conditions in patients with type I diabetes mellitus. The probe exhibits remarkable changes in absorption spectra and fluorescence enhancement under acidic conditions, showing good linearity with pH variations and potential applications in acidic microenvironments.
Article
Biology
Merry Chen, Julie Vincent, Alexis Ezeanii, Saurabh Wakade, Shobha Yerigenahally, Danielle E. Mor
Summary: This study demonstrates the mechanisms of gut-derived alpha-synuclein propagation and neurodegeneration using Caenorhabditis elegans models, and shows that RNAi knockdown can protect against this process.
LIFE SCIENCE ALLIANCE
(2022)
Article
Chemistry, Multidisciplinary
Fabian Steininger, Niels Peter Revsbech, Klaus Koren
Summary: A dipping probe total dissolved inorganic carbon (DIC) microsensor has been developed based on a localized acidic microenvironment, allowing for conversion of bicarbonate and carbonate to CO2. The sensor shows a linear response over a wide range and has the potential for high spatial and temporal resolution measurements of DIC in environmental sensing.
Article
Chemistry, Analytical
Yuchi Cheng, Huangmei Zhou, Jinming Xu, Yu Zhao, Xihang Chen, Rodolphe Antoine, Meng Ding, Kun Zhang, Sanjun Zhang
Summary: A two-photon excited ratiometric fluorescent pH sensor was developed by combining L-cysteine-protected AuNCs (Cys@AuNCs) with fluorescein isothiocyanate (FITC). The sensor showed a large dynamic range for pH measurement and had potential for sensitive quantitation of pH in living cells under two-photon excitation. In addition, the synthesized Cys@AuNCs exhibited excellent photoactivated peroxidase-like activity, promising for rapid colorimetric biosensing and control of catalytic reactions by photostimulation.
Review
Chemistry, Analytical
Muhanad Al-Jeda, Emmanuel Mena-Morcillo, Aicheng Chen
Summary: This article reviews the design and fabrication technologies of micro-/nano-sized pH sensors, as well as their applications based on SECM. Considerations for selecting sensing probes and different types of pH sensitive probes are discussed. The future trends and emerging applications of micro-/nano-sized pH sensors are also addressed.
Article
Materials Science, Multidisciplinary
S. Bonyadi, Kh Ghanbari
Summary: In this study, a carbon paste electrode modified with PMEL-PASP/ CPE copolymer film was used for selective determination of dopamine, exhibiting high electrocatalytic activity, low detection limit, and satisfactory performance in human blood serum. Various characterization techniques were employed, and the sensor showed acceptable reproducibility, repeatability, and long-term stability.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Analytical
Hao Kang, Wei Shu, Jin Yu, Mengxu Gao, Rubing Han, Jing Jing, Rubo Zhang, Xiaoling Zhang
Summary: Parkinson's disease is a common neurodegenerative disease that causes great pain and economic burden. Peroxynitrite (ONOO-) plays a significant role in the development of Parkinson's disease, but effective detection methods are currently lacking. This study design a highly sensitive and selective near-infrared ratiometric fluorescent probe to track ONOO- and successfully observe its changes in a Parkinson's disease model.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Applied
Guiqian Fang, Rang Wang, Dongxue Zhan, Gaofei Chen, Yujun Huang, Zihan Ma, Qingqiang Yao, Zhongyu Wu
Summary: A facile hemicyanine-naphthalene-based fluorescent sensor was constructed for colorimetric and ratiometric sensing of pH through a simple synthesis process. Sensor 3 exhibited high sensitivity to extremely alkaline solutions and had a notably large Stokes shift. The fluorescence intensity increased significantly in the pH range of 5.0–12.0, making it a promising tool for detecting extremely alkaline solutions.
Article
Metallurgy & Metallurgical Engineering
Xin Su, Can-jun Liu, Yang Liu, Ya-hui Yang, Xuan Liu, Shu Chen
Summary: A versatile phase transformation strategy was proposed to synthesize novel BiVO4 nanosheets@WO3 nanorod and nanoplate arrays films. The prepared WO3/BiVa(4) heterojunction films exhibited excellent PEC activity in water splitting, with photocurrent densities reaching up to about 1.56 and 1.20 mA/cm(2) under visible light illumination without cocatalyst.
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
(2021)
Article
Chemistry, Analytical
Chenchen Li, Yi Zhuo, Xia Xiao, Shuangfu Li, Kai Han, Ming Lu, Jiaxin Zhang, Shu Chen, Hui Gu
Summary: A reliable method for in vivo measurements of chloride ion (Cl-) in the brain using an electrochemical microbiosensor (ECMB) was developed, with high specificity, accuracy, and reproducibility for real-time monitoring of Cl- in Parkinson's disease (PD) mouse brain. It showed promising availability and reliability compared to the standard Volhard's method.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Ting Li, Hui Dong, Yuanqiang Hao, Yintang Zhang, Shu Chen, Maotian Xu, Yanli Zhou
Summary: NIR light-driven PEC sensing is a promising analytical technique for in situ bioanalysis due to its deep penetration capability and minimal invasiveness. Recent developments in NIR PEC sensors focus on light-responsive materials and applications, with potential for significant future advancements.
Article
Chemistry, Analytical
Yuanqiang Hao, Yintang Zhang, Qiuling Sun, Shu Chen, Zilong Tang, Rongjin Zeng, Maotian Xu
Summary: Hypochlorous acid (HOCl) is a potent reactive oxygen species essential in biological processes. A new ratiometric fluorescent probe has been designed and synthesized for sensitive and selective detection of HOCl, with advantages including rapid response, high signal to noise ratio, and utility for visualizing HOCl in cells and organisms.
MICROCHEMICAL JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Canjun Liu, Linmei Chen, Xin Su, Shu Chen, Jie Zhang, Heping Yang, Yong Pei
Summary: Constructing a heterojunction interface in TiO2/BiVO4 photoanode significantly improves its PEC performance, with the in situ transformation method exhibiting better results than the soaking-calcining method. The faster electron transfer rate in the heterojunction interface and higher Fermi energy level on the BiVO4-202 surface contribute to the enhanced photocurrent density under visible light irradiation without a cocatalyst.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Zhi-bei Qu, Yimin Jiang, Jiaxin Zhang, Shu Chen, Rongjin Zeng, Yi Zhuo, Ming Lu, Guoyue Shi, Hui Gu
Summary: The study investigated the changes of ascorbic acid (AA) in Parkinson's disease (PD) using an electrochemical approach and established a new platform for ratiometric electrochemical measurements of AA with high sensitivity, excellent selectivity, and reproducibility. The in vivo determination of AA levels in different regions of mouse brains demonstrated a significant decrease in AA levels in the hippocampus and cortex of PD mice compared to normal mice.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chong-Hua Zhang, Shenglan Wang, Peisheng Zhang, Shuaiwei Xu, Zhi-ling Song, Jian Chen, Shu Chen, Rongjin Zeng
Summary: The study developed a dual-targeting and tumor microenvironment responsive nanoassembly for imaging of HAase and PDT of tumor. The nanoassembly could be efficiently endocytosed into targeted tumor cells and activated both fluorescence signal and enhanced PDT effect by highly expressed HAase.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Ting Li, Yuanqiang Hao, Hui Dong, Chunlan Li, Jiaxiang Liu, Yintang Zhang, Zilong Tang, Rongjin Zeng, Maotian Xu, Shu Chen
Summary: This paper proposes a new strategy for PEC analysis by in situ generation of organic photosensitizers, achieving highly selective turn-on PEC response. The proposed strategy is expected to expand the paradigm of PEC sensing and holds potential for detecting various other analytes.
Article
Electrochemistry
Jin Ma, Wan Wu, Xi Xiao, Yaqian Feng, Yuanqiang Hao, Jie Zhang, Canjun Liu, Peisheng Zhang, Jian Chen, Rongjin Zeng, Shu Chen
Summary: In this study, electrodeposition of polymelamine (pMel) was achieved at relatively low onset potentials in a melamine aqueous solution containing trace bromide ion (Br). The addition of Br greatly promoted the polymerization reaction. Various techniques were used to investigate the effects of Br on the electropolymerization process, and a pMel film was successfully deposited on gold substrates.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yu Luo, Ruizhi Lin, Yimei Zuo, Ziyi Zhang, Yi Zhuo, Ming Lu, Shu Chen, Hui Gu
Summary: In this study, an electrochemical microsensor was designed for the selective and accurate measurement of hydrogen peroxide (H2O2) in the living brain. By tuning the structure of the probe and using a stable internal reference material, the sensor was able to track the concentration of H2O2 in real-time. The sensor was successfully applied to measure H2O2 in the Parkinson's disease mouse brain. This research provides insights into the role and impact of H2O2 in Parkinson's disease.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yimei Zuo, Sisi Wang, Ruizhi Lin, Gelei Xiao, Shu Chen, Rongjin Zeng, Hui Gu
Summary: A portable electrochemical microsensor was developed to simultaneously quantify dissolved oxygen and hydrogen sulfide. Pd@Ni nanoparticles were used for the detection of O2 and H2S, achieving high selectivity. The microsensor showed a measurement range of 36-318 μM for O2 and 0.1-2.5 μM for H2S.
Article
Chemistry, Analytical
Xia Xiao, Chenchen Li, Yuzhi Liu, Yaqian Feng, Kai Han, Haoyue Xiang, Guoyue Shi, Hui Gu
Summary: A ratiometric electrochemical microsensor (REM) was developed for in vivo measurement of cerebral Cl- by utilizing uniform Ag nanoparticles and methylene blue to achieve high sensitivity, selectivity, stability, reproducibility, and anti-biofouling.
Article
Chemistry, Analytical
Shenglan Wang, Chong-Hua Zhang, Peisheng Zhang, Shu Chen, Zhi-ling Song, Jian Chen, Rongjin Zeng
Summary: A novel AIE-dot-based nanoprobe was proposed for hyaluronidase detection, exhibiting high sensitivity, excellent selectivity, and anti-interference capabilities. The nanoprobe offers a practicable turn-on strategy for specific fluorescence imaging of HAase in live tumor cells, showing promise for recognition and imaging of HAase in tumors in biological research.
ANALYTICAL METHODS
(2021)