Article
Cell Biology
Emily S. Wilson, Karen Litwa
Summary: Hyaluronan synthase-2 is crucial in the development of neural circuits, with increased hyaluronan production reducing excitatory synapses, promoting inhibitory synaptogenesis, and suppressing action potential formation. The hyaluronan receptor, CD44, plays a role in hyaluronan retention and regulation of excitatory synaptogenesis through RhoGTPase signaling.
Article
Biochemical Research Methods
Rebecca MacLeod, Fok Vun Chan, Han Yuan, Xin Ye, Yun Jin Ashley Sin, Teraesa M. Vitelli, Tudor Cucu, Annie Leung, Irene Baljak, Samantha Osinski, Yuhong Fu, Gyu Ik Daniel Jung, Anant Amar, Paul L. DeAngelis, Urban Hellman, Mary K. Cowman
Summary: A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ~9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zhengliang L. Wu, James M. Ertelt
Summary: The study focused on assays for endoglycosidases and demonstrated the substrate specificities and activities of HYALs under different pH conditions. It was also found that the activities of HYALs must be strictly regulated by pH levels. The research suggests the potential of designing novel therapeutics by targeting these important enzymes.
Article
Polymer Science
Annalisa La Gatta, Emiliano Bedini, Maria Aschettino, Rosario Finamore, Chiara Schiraldi
Summary: This study investigated the rheological properties and stability of four available HA-BDDE hydrogels and their relationship to the type and degree of HA structural modification. The results showed that most of the HA in the gels was branched, and there was no correlation between the conventionally measured rheological properties and the chemical data. The hydration process and the amount of crosslinked HA significantly affected the rheology of the gels. These findings contribute to a better understanding of these gels and provide valuable information for their design and characterization.
Article
Chemistry, Applied
Lin Pan, Xuze Ai, Tianyu Fu, Li Ren, Qingsen Shang, Guoyun Li, Guangli Yu
Summary: Hyaluronan can be readily fermented by human gut microbiota, boosting certain bacterial species and producing significant amounts of short-chain fatty acids. The fermentation products indicate that hyaluronan can be degraded into specific oligosaccharides.
CARBOHYDRATE POLYMERS
(2021)
Review
Pharmacology & Pharmacy
Stavros Garantziotis
Summary: The extracellular matrix plays a vital role in maintaining homeostasis and disease development. Recent research has shown the therapeutic potential of targeting hyaluronan, a key component of the extracellular matrix, in treating inflammatory and immune diseases. This review summarizes the current state of the art and explores the challenges and opportunities in developing matrix biologics as a new class of therapeutic agents.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Ana Obaha, Marko Novinec
Summary: This comprehensive review explores the intricate regulation of peptidase activity in human health and disease, focusing on mechanisms such as exosites, allosteric sites, and zymogen activation. The review also discusses the influence of glycosaminoglycans and non-inhibitory binding proteins on enzyme activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Petra Zadnikova, Romana Sinova, Vojtech Pavlik, Matej Simek, Barbora Safrankova, Martina Hermannova, Kristina Nesporova, Vladimir Velebny
Summary: This study characterizes the distribution and activity of hyaluronidases and other proteins involved in the degradation of hyaluronan (HA) in healthy human skin. It also identifies a novel hyaluronidase TMEM2 and a protein CEMIP involved in HA degradation, which were localized in the human epidermis and dermis.
Article
Chemistry, Applied
Radovan Buffa, Tomas Klejch, Martina Hermannova, Lenka Hejlova, Vit Svozil, Hana Vagnerova, Hana Skubalova, Kristina Nesporova, Vladimir Velebny
Summary: A mild and efficient reduction method was developed to convert the negatively charged glucuronate units of hyaluronic acid (HA) into less polar glucose units without severe polymer degradation. The resulting derivative, HA-Red, showed higher stability compared to HA, with stability dependent on the degree of modification. Preliminary in vitro studies demonstrated no negative effects of HA-Red on fibroblast growth, suggesting potential applications for biodegradable and biocompatible HA derivatives with increased resistance to degradation.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Yun Jin Ashley Sin, Rebecca MacLeod, Adam P. Tanguay, Andrew Wang, Olivia Braender-Carr, Teraesa M. Vitelli, Gregory D. Jay, Tannin A. Schmidt, Mary K. Cowman
Summary: The size, conformation, and organization of glycosaminoglycan hyaluronan affect its interactions with proteins. AlphaLISA assay technology can be used to study protein-mediated networking of hyaluronan. The study identified several substances, including heparin and proteoglycan 4, as inhibitors of HA crosslinking.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, Research & Experimental
Shengyuan Zheng, Senbo An, Yan Luo, Djandan Tadum Arthur Vithran, Shaoqu Yang, Bangbao Lu, Zhenhan Deng, Yusheng Li
Summary: HYBID is a new enzyme that degrades hyaluronan and is present in various cells of the human body. Recent research has found that HYBID is over-expressed in chondrocytes and synoviocytes associated with osteoarthritis. High levels of HYBID are significantly correlated with cartilage degeneration and hyaluronic acid degradation in the joints. Additionally, HYBID can contribute to inflammatory responses and tissue fibrosis in the joints, exacerbating osteoarthritis. Understanding the role of HYBID in osteoarthritis could provide new insights for treatment strategies.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Endocrinology & Metabolism
Mohd Shazli Draman, Lei Zhang, Colin Dayan, Marian Ludgate
Summary: Graves' orbitopathy (GO) is a complex disease characterized by extensive remodeling of orbital tissue, with potential involvement of autoantigens such as TSHR and IGF1R. Research in the past decade has provided further insights into the pathogenesis of GO.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Engineering, Biomedical
Austen L. Michalak, Greg W. Trieger, Kelsey A. Trieger, Kamil Godula
Summary: Extracellular glycans, such as GAGs, play a crucial role in tissue development and maintenance by regulating growth factor gradients and promoting the formation of signaling complexes at the cell surface. The study focuses on utilizing a stem cell array platform to analyze the contributions of chemically modified GAG polysaccharides to signaling pathways in mouse embryonic stem cells. Advances in synthetic and recombinant technologies provide new opportunities to study growth factor binding and signaling in response to the presence of biologically active components like GAGs.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Li Chen, Kaikai Shi, Nicholas Ditzel, Weimin Qiu, Florence Figeac, Louise Himmelstrup Dreyer Nielsen, Michaela Tencerova, Justyna Magdalena Kowal, Ming Ding, Christina Moller Andreasen, Thomas Levin Andersen, Moustapha Kassem
Summary: The levels of KIAA1199, a factor secreted by bone marrow stromal cells, are associated with the risk of osteoporotic fracture. KIAA1199 deficiency enhances bone formation, accelerates bone healing, and protects against ovariectomy-induced bone loss. Transplanted skeletal stem cells generate secreted factors that regulate bone regeneration, and KIAA1199 is identified as one of these factors. Patients with higher plasma levels of KIAA1199 have a higher risk of osteoporotic fracture, while lower expression levels of KIAA1199 in patient bone marrow stromal cells are associated with reduced osteogenic differentiation potential. In vitro and in vivo experiments show that KIAA1199-deficient bone marrow stromal cells exhibit enhanced osteoblast differentiation and ectopic bone formation. KIAA1199 knockout mice have increased bone mass, biomechanical strength, bone formation rate, and accelerated healing of surgically generated bone defects, and are protected from ovariectomy-induced bone loss. Mechanistically, KIAA1199 regulates osteogenesis by inhibiting the production of osteopontin by osteoblasts through integrin-mediated AKT and ERK-MAPK signaling pathways. Therefore, KIAA1199 is a regulator of osteoblast differentiation and bone regeneration, and could be a potential target for treating or managing low bone mass conditions.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Sean P. Giblin, Sashini Ranawana, Shyreen Hassibi, Holly L. Birchenough, Kyle T. Mincham, Robert J. Snelgrove, Tomoko Tsuchiya, Shiro Kanegasaki, Douglas Dyer, James E. Pease
Summary: In this study, the structural and chemoattractant credentials of CXCL17 were evaluated, revealing its difference from chemokines but its ability to bind GAGs and modulate chemotactic responses to another chemokine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
M. Gronbach, F. Mitrach, V. Lidzba, B. Mueller, S. Moeller, S. Rother, J. Salbach-Hirsch, L. C. Hofbauer, M. Schnabelrauch, V. Hintze, M. C. Hacker, M. Schulz-Siegmund
ACTA BIOMATERIALIA
(2020)
Article
Biochemistry & Molecular Biology
Christiane Heinemann, Josephine Adam, Benjamin Kruppke, Vera Hintze, Hans-Peter Wiesmann, Thomas Hanke
Summary: Different conventional and novel methods of detachment in combination with different culture surfaces were investigated to obtain optimal osteoclast differentiation, yield, and vitality rates without loss of function. The innovative method-using thermoresponsive surfaces for cultivation and detachment-was found to be best suited for maintaining osteoclast activity. This provides biomaterial researchers with a novel tool for material characterization, showing that osteoclasts can be predifferentiated and transferred under preservation of osteoclastic resorption activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Matthias Schnabelrauch, Juergen Schiller, Stephanie Moeller, Dieter Scharnweber, Vera Hintze
Summary: The review discusses recent achievements in chemically modified GAG and collagen/GAG-based coatings and hydrogels, emphasizing the regulatory role of GAG in tissue regeneration and their potential as promising candidates for controlling cellular responses.
BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Kanagasabai Balamurugan, Linda Koehler, Jan-Niklas Duerig, Ute Hempel, Joerg Rademann, Vera Hintze, M. Teresa Pisabarro
Summary: Angiogenesis is a crucial physiological process involving VEGF and PDGF, with hyaluronan (HA) based GAG interacting with PDGF and its receptor PDGFR-beta. Computational and experimental analyses suggest that HA derivatives may play a role in modulating the PDGF/PDGFR-beta signaling system in angiogenesis and related diseases.
BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Ulf Anderegg, Norbert Halfter, Matthias Schnabelrauch, Vera Hintze
Summary: This review discusses the enhancement of skin regeneration processes by collagen (coll)/glycosaminoglycan (GAG) matrices containing sulfated GAG (sGAG) in various in vitro models, through regulating cell migration and growth, stimulating melanogenesis, and enhancing epithelial differentiation. These matrices have the potential to deliver growth factors and act as bioinspired microenvironments for skin regeneration.
BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lysann M. Kroschwald, Felix Allerdt, Anne Bernhardt, Sandra Rother, Kai Zheng, Iram Maqsood, Norbert Halfter, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Michael C. Hacker, Stefan Rammelt, Aldo R. Boccaccini, Vera Hintze
Summary: The study demonstrates that aECM containing BGN can promote osteogenic differentiation of hMSC while delaying cellular growth, with a stimulating effect on ALP activity and calcium deposition. Some donors show a synergistic effect between sGAG and BGN, contributing to bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Sandra Rother, Norbert Halfter, Karen M. Fiebig, Juliane Moritz, Stephanie Moeller, Matthias Schnabelrauch, Charles James Kirkpatrick, Robert Sader, Hans-Peter Wiesmann, Dieter Scharnweber, Vera Hintze, Shahram Ghanaati
Summary: This study aimed to functionalize a collagen-based scaffold with high-sulfated hyaluronan and investigate its properties in vitro and in vivo. The results showed that covalent binding of high-sulfated hyaluronan to collagen released only a marginal amount of the hyaluronan, while electrostatic binding released more. The covalent binding scaffolds showed reduced release of vascular endothelial growth factor and transforming growth factor beta 1, but increased release of interleukin-8 and epithelial growth factor compared to other scaffolds. In vivo, the covalent binding scaffolds reduced the proinflammatory macrophage response and did not induce multinucleated giant cell formation, while maintaining a stable structure and integration into the implantation region. This functionalized collagen scaffold has potential as a stable biomaterial for full-thickness skin regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Poh Soo Lee, Christiane Heinemann, Kai Zheng, Revathi Appali, Franziska Alt, Jan Krieghoff, Anne Bernhardt, Aldo R. Boccaccini, Ursula van Rienen, Vera Hintze
Summary: This study investigates the effects of bioactive glass nanoparticles (BGN) and electric field (EF) stimulation on osteogenic differentiation of human mesenchymal stem cells (hMSC). The results demonstrate that BGN and EF have synergistic effects on collagen structure and cell proliferation, and they activate different signaling pathways to promote osteogenic differentiation.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Gloria Ruiz-Gomez, Juliane Salbach-Hirsch, Jan-Niklas Duerig, Linda Koehler, Kanagasabai Balamurugan, Sandra Rother, Sophie-Luise Heidig, Stephanie Moeller, Matthias Schnabelrauch, Giulia Furesi, Sophie Paehlig, Pedro M. Guillem-Gloria, Christine Hofbauer, Vera Hintze, M. Teresa Pisabarro, Joerg Rademann, Lorenz C. Hofbauer
Summary: The WNT signaling pathway is important for bone development and regeneration, and abnormalities in WNT ligands and inhibitors are associated with various bone diseases. This study focused on glycosaminoglycan (GAG) recognition by DKK1, a WNT inhibitor, and aimed to develop WNT signaling regulators. Through a multidisciplinary approach, researchers designed and synthesized GAG derivatives with improved neutralizing properties for DKK1. These derivatives showed increased WNT pathway activity and improved bone regeneration in experimental models. The findings suggest that rationally engineered GAG variants could be used as novel therapeutic approaches.
Article
Materials Science, Biomaterials
David Kilian, Aayush Poddar, Vanessa Desrochers, Christiane Heinemann, Norbert Halfter, Suihong Liu, Sandra Rother, Michael Gelinsky, Vera Hintze, Anja Lode
Summary: The combination of a highly viscous alginate-methylcellulose (algMC) bioink with collagen-based artificial extracellular matrix (aECM) is a controllable and tailorable system that enhances adhesion of stem cells and differentiation of chondrocytes. The addition of the stabilizer, tannic acid (TA), improves interaction with the microenvironment and promotes chondrogenic matrix production. However, cytotoxicity is observed when TA concentrations exceed a certain amount.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Norbert Halfter, Eva Espinosa-Cano, Gloria Maria Pontes-Quero, Rosa Ana Ramirez-Jimenez, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Hans-Peter Wiesmann, Vera Hintze, Maria Rosa Aguilar
Summary: The limitations of current wound dressings for chronic wounds require the development of new approaches. One promising approach is the immune-centered approach, which aims to restore the regenerative and anti-inflammatory characteristics of macrophages. Ketoprofen nanoparticles (KT NPs) have shown potential in reducing inflammation and increasing anti-inflammatory cytokines in macrophages. This study investigated the incorporation of KT NPs into hyaluronan/collagen-based gels and their effects on cell viability, gel morphology, and inflammatory markers. The results suggest that HA/collagen-based gels containing KT NPs could be a novel therapeutic approach for chronic wounds, but further testing is needed to determine their efficacy in skin regeneration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Christiane Heinemann, Frauke Buchner, Poh Soo Lee, Anne Bernhardt, Benjamin Kruppke, Hans-Peter Wiesmann, Vera Hintze
Summary: Biopolymer hydrogels, including hyaluronan (HA) and/or gelatin (GEL)-based hydrogels, are sensitive to sterilization and their physicochemical properties can be affected by sterilization methods. gamma irradiation and supercritical carbon dioxide (scCO(2)) treatment were compared in this study. Gamma irradiation increased the degradation of methacrylated HA and decreased the elastic modulus of the hydrogels, while scCO(2) treatment had a negative effect on cell proliferation and osteogenic differentiation. Gamma-irradiated methacrylated GEL/HA hydrogels showed promise as multi-component bone substitute materials.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Sandra Rother, Gloria Ruiz-Gomez, Kanagasabai Balamurugan, Linda Koehler, Karen M. Fiebig, Vanessa D. Galiazzo, Ute Hempel, Stephanie Moeller, Matthias Schnabelrauch, Johannes Waltenberger, M. Teresa Pisabarro, Dieter Scharnweber, Vera Hintze
Summary: Innovative biomaterial concepts are required to restore the regeneration capacity of large-size vascularized tissue defects, with chemically modified sGAG derivatives playing a key role in controlling and tuning VEGF(165) function. Hydrogels containing sHA-AC showed significant effects on stimulating endothelial cell proliferation and morphology extension, potentially improving the healing of injured vascularized tissues.
ACS APPLIED BIO MATERIALS
(2021)
Article
Materials Science, Biomaterials
Yvonne Foerster, Sabine Schulze, Anja Penk, Christin Neuber, Stephanie Moeller, Vera Hintze, Dieter Scharnweber, Matthias Schnabelrauch, Jens Pietzsch, Daniel Huster, Stefan Rammelt
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
