Circular RNA circFOXP1 promotes angiogenesis by regulating microRNA-127-5p/CDKN2AIP signaling pathway in osteosarcoma

Osteosarcoma is known to have a high metastatic potential, which is closely related to angiogenesis. circRNAs are closely associated with osteosarcoma metastasis. This study aims to investigate the role of Circular RNA circFOXP1 in angiogenesis in osteosarcoma. We detected circFOXP1 expression in osteosarcoma, as well as its prognostic value. Tube formation assay and immunohistochemistry staining were conducted to determine the condition of tube formation. RT-qPCR was performed to explore targeted genes. Luciferase reporter assays were carried out to explore the interaction between miR-127-5p, ircFOXP1, and CDKN2AIP, respectively. In vivo studies further confirmed the relationship between circFOXP1 and tumor angiogenesis in osteosarcoma. We found that circFOXP1 expression was increased in osteosarcoma, and could promote angiogenesis in osteosarcoma through upregulating CDKN2AIP expression. Moreover, circFOXP1 could directly bind to miR-127-5p, which further targets CDKN2AIP directly. In conclusion, circFOXP1 promoted angiogenesis by regulating miR-127-5p/CDKN2AIP signaling pathway in osteosarcoma.

Automated summary

In this study, circFOXP1 was found to promote angiogenesis in osteosarcoma by regulating the miR-127-5p/CDKN2AIP signaling pathway. It upregulated CDKN2AIP expression to enhance tumor angiogenesis, and could directly bind to miR-127-5p to indirectly affect CDKN2AIP expression. These findings highlight the role of circFOXP1 in osteosarcoma metastasis and provide insights into potential therapeutic targets for this aggressive cancer.