Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Article
Materials Science, Biomaterials
Jorge A. Tavares-Negrete, Sara Cristina Pedroza-Gonzalez, Ada I. Frias-Sanchez, Miriam L. Salas-Ramirez, Maria de los Angeles de Santiago-Miramontes, Claudia Maribel Luna-Aguirre, Mario M. Alvarez, Grissel Trujillo-de Santiago
Summary: A simple and cost-effective strategy involving GelMA hydrogels supplemented with minimally processed tissue (MPT) was developed to fabricate densely packed skeletal-muscle-like tissues. Incorporating 0.5-2.0% (w/v) of MPT into GelMA hydrogels enhanced the proliferation of myoblast cells compared to other GelMA hydrogels. The addition of MPT provided biochemical and topological cues, such as growth factors and structurally preserved proteins, and showed suitable rheological properties for extrusion bioprinting.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Analytical
Bo Yang, Tianqi Liu, Ge Gao, Xianglin Zhang, Bin Wu
Summary: This study explores the GelMA/Agarose suspension printing system and defines a suitable printing window. The resulting scaffolds have good water absorption and elasticity but exhibit significant deformation during printing.
Article
Engineering, Manufacturing
Huijun Li, Yu Jun Tan, Raj Kiran, Shu Beng Tor, Kun Zhou
Summary: This study introduces two extrusion-based bioprinting approaches using a combination of cell-laden GelMA and Alg/MC, which effectively produce structures with geometric complexity while maintaining high structural integrity and cell viability. The methods involve alternate layer-by-layer printing and submerging the extrusion nozzle into a bath of Alg/MC to demonstrate improved printability and cell protection during UV exposure.
ADDITIVE MANUFACTURING
(2021)
Article
Engineering, Biomedical
Yuan Sun, Kang Yu, Jing Nie, Miao Sun, Jianzhong Fu, Huiming Wang, Yong He
Summary: This study introduces the research on digital light processing bioprinting, establishing a photo crosslinking theory for ink and adding light absorber to control crosslinking depth. A standardized methodology for quantitatively evaluating printing resolution was developed, and complex biostructures were successfully printed and mechanical properties adjusted.
Article
Nanoscience & Nanotechnology
Shreya Mehrotra, Rishabh Deo Singh, Ashutosh Bandyopadhyay, G. Janani, Souradeep Dey, Biman B. Mandal
Summary: The study utilized a bioink containing carbon nanotubes for bioprinting, showing its regulatory effects on cardiac biomarkers and importance in maintaining cell viability. Through microsphere injection and in vivo implantation experiments, it was found that modulation towards anti-inflammatory M2 phenotype, can extend the time frame for dealing with hostile microenvironments to promote host neovascularization and implant acceptance.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Dermatology
Hongyu Wang, Jiaming Wan, Zhiqiang Zhang, Ruixing Hou
Summary: Advancements in GelMA hydrogels are revolutionizing regenerative medicine and tissue engineering, with its potential applications in wound healing and skin regeneration. Challenges remain in scaling up 3D bioprinting techniques and developing advanced bioinks.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Hao Wang, Yuxin Guo, Yugang Hu, Yanxiang Zhou, Yueying Chen, Xin Huang, Jinling Chen, Qing Deng, Sheng Cao, Bo Hu, Riyue Jiang, Juhong Pan, Tuantuan Tan, Yijia Wang, Yun Chen, Qi Dong, Pu Chen, Qing Zhou
Summary: Heart failure is a critical phase of cardiovascular disease that leads to disability and mortality. Myocardial infarction is a significant cause of heart failure, but its management remains difficult. A 3D bio-printed cardiac patch has emerged as a promising approach to replace damaged cardiomyocytes. In this study, we aimed to improve cell survival inside the bio-3D printed patch by constructing acoustically sensitive nano oxygen carriers. The results showed that the ultrasound-triggered phase transition of nanodroplets and the encapsulation of hemoglobin improved cell survival and cardiac function, making this a potential treatment for myocardial infarction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Kang Yu, Xinjie Zhang, Yuan Sun, Qing Gao, Jianzhong Fu, Xiujun Cai, Yong He
Summary: Projection-based 3D bioprinting (PBP) is suitable for printing delicate structures, but the requirements for printing details with photocurable bioink are not well understood. This study systematically investigates the printing details using GelMA-based bioink as an example, establishes quantized models for evaluating printing errors, and presents better strategies for bioprinting different structures.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Pei Zhuang, Zachary Greenberg, Mei He
Summary: A novel 3D printable nanocomposite starch hydrogel was developed with enhanced biocompatibility for promoting 3D cell growth, utilizing gelatin nanoparticles and collagen. The unique rheological properties of the hydrogel, along with the homogeneous microporous structure containing collagen fibers and gelatin nanoparticles, support cell attachment and proliferation. The cell metabolic activity study confirmed the remarkable enhancement of biological function of the developed starch hydrogel, making it a highly desirable bio-ink for advancing 3D tissue engineering.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biochemistry & Molecular Biology
Hitendra Kumar, Kabilan Sakthivel, Mohamed G. A. Mohamed, Emilie Boras, Su Ryon Shin, Keekyoung Kim
Summary: Optimized synthesis parameters were used to develop GelMA bioinks with slow sol-gel transition at room temperature and visible light crosslinkable functions. Eight GelMA combinations suitable for DLP-SLA bioprinting were identified through systematic physical and rheological property characterizations. RO-GelMA bioinks were found to be most suitable for achieving high resolution in DLP-SLA printing, showing excellent biocompatibility and cell survival rates.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Engineering, Biomedical
Caterine Yesenia Carrasco Montesdeoca, Thiago Domingues Stocco, Fernanda Roberta Marciano, Thomas J. Webster, Anderson Oliveira Lobo
Summary: Three-dimensional bioprinting is a powerful technique for manufacturing improved engineered tissues. Recent advances in the 3D bioprinting of hydrogels, particularly GelMA hydrogels, have significantly advanced the field of cartilage tissue repair. These hydrogels have optimal chemical and physical properties that allow them to respond to external stimuli and release bioactive agents, promoting desired tissue responses.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Pharmacology & Pharmacy
Elisa Marozzi Cruz, Lucas Simoes Machado, Laura Nicoleti Zamproni, Larissa Valdemarin Bim, Paula Scanavez Ferreira, Leonardo Alves Pinto, Luiz Antonio Pessan, Eduardo Henrique Backes, Marimelia Aparecida Porcionatto
Summary: Neuronal loss is a major challenge in regenerative medicine, and providing a suitable microenvironment for neuron replacement is crucial. Gelatin methacrylate (GelMA) is an excellent biomaterial due to its biocompatibility and degradation properties. This study aims to develop GelMA-based bioinks and evaluate their ability to support neurogenesis. Incorporating extracellular matrix components, such as Geltrex (TM), is essential for promoting human neuroprogenitor cell viability. Geltrex (TM): 8% GelMA (1:1) bioink effectively maintains stemness and promotes neuronal differentiation.
Article
Nanoscience & Nanotechnology
Carmen Radeke, Raphael Pons, Marko Mihajlovic, Jonas R. Knudsen, Sarkhan Butdayev, Paul J. Kempen, Charis-Patricia Segeritz, Thomas L. Andresen, Christian K. Pehmoller, Thomas E. Jensen, Johan U. Lind
Summary: In order to achieve the automated fabrication of complex tissue mimicking constructs through 3D bioprinting, it is necessary to develop bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Here, we present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. By tailoring the size and dispersibility of cellulose fibrils, we are able to create nanofibers that match the size and dimensions of natural collagen fibers, allowing for the orientation of cells and the spatial organization of engineered tissues during printing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Chenmin Wang, John Robert Honiball, Junyu Lin, Xingyu Xia, Dzi Shing Aaron Lau, Bo Chen, Lianfu Deng, William Weijia Lu
Summary: In this study, a novel printing technique called infiltration-induced suspension bioprinting (IISBP) based on a hyaluronic acid (HA) suspension system was described. The IISBP technique allows the modulation of 3D printed hydrogel scaffolds properties through the infiltration action. This technique is expected to meet the scaffolds requirements of a wide range of cell culture conditions in bioprinting applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Materials Science, Characterization & Testing
Anne Blair Heidelberg, Michele Lanotte, M. Emin Kutay
Summary: The characteristics of shape, angularity, and surface texture of aggregates significantly impact the long-term performance of pavement materials. Current manual and subjective test methods have limitations, leading engineers to explore digital image analysis techniques. However, cost and differing definitions hinder result comparability. This paper proposes a new methodology using smartphone cameras and spherical harmonic modeling to measure aggregate morphological indices, which could potentially inform new standards for transportation materials.
JOURNAL OF TESTING AND EVALUATION
(2021)
Article
Polymer Science
Luca Desidery, Michele Lanotte
Summary: The study found that the type and form of polyolefin have a significant impact on the mechanical response time of the composite material during the blending process of polyethylene, polypropylene, and bitumen. Polyethylene shows higher affinity with bitumen and produces modified bitumen with acceptable performance after blending for 210 minutes.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Engineering, Civil
Aksel Seitllari, Michele Lanotte, M. Emin Kutay
Summary: This study quantified the effects of testing and calibration variables on asphalt concrete rutting prediction through laboratory tests and mathematical analysis, showing differences in results ranging from 0% to 80% when using different methods of analysis. Additionally, the variability of rutting prediction in warm mix asphalts was found to be higher than in hot mix asphalts.
JOURNAL OF TRANSPORTATION ENGINEERING PART B-PAVEMENTS
(2021)
Article
Polymer Science
Stefano Marini, Michele Lanotte
Summary: The study suggests that rubberized asphalt may not improve rutting performance in 'lean' asphalt mixtures, but it performs better in resisting fatigue cracking. The gradation of aggregates plays a major role in material response, with gap-graded mixtures often outperforming dense-graded ones.
Article
Polymer Science
Luca Desidery, Michele Lanotte
Summary: The practice of replacing traditional polymer modifiers with waste plastics has gained increasing interest in recent years, especially with polyethylene-based plastics showing promising potential in bitumen modification. Additionally, the chain length of waxes, the form of added waste polyethylene, and the blending protocol have critical effects on the properties of the mixture.
Article
Green & Sustainable Science & Technology
Muhammad Jamal, Michele Lanotte, Filippo Giustozzi
Summary: This study investigates the effects of UV radiation on the chemo-mechanical properties of asphalt, comparing it to thermal aging. The results show that thermal aging affects the chemical bonds and rheological properties of asphalt, while UV aging mainly targets hydrocarbon chains. Asphalt modified with crumb rubber shows greater resistance to both UV and thermal aging.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Multidisciplinary
Deema Islayem, Fatima Ba Fakih, Sungmun Lee
Summary: Excessive production of reactive oxygen species (ROS) is implicated in aging-related disorders. However, monitoring ROS in vivo remains challenging due to their short half-lives. In this study, three colorimetric methods were compared for their specificity and sensitivity in detecting malondialdehyde (MDA), a biomarker of lipid oxidation induced by ROS. The results showed that the MPI method was sensitive enough to detect MDA in diseased conditions.
Article
Construction & Building Technology
Seyed Farhad Abdollahi, Michele Lanotte, M. Emin Kutay, Hussain Bahia
Summary: Many road agencies still use the outdated AASHTO 1993 pavement design method, while this study proposes a new AASHTO 93 Plus method to determine asphalt mixture's structural layer coefficient, which aligns better with field data.
INTERNATIONAL JOURNAL OF PAVEMENT ENGINEERING
(2022)
Article
Materials Science, Characterization & Testing
Ezio Santagata, Pier Paolo Riviera, Orazio Baglieri, Michele Lanotte
Summary: The increasing need for environment protection has driven road agencies to adopt sustainable technologies, including the use of recycled materials and wastes. This experimental investigation explores the performance-related properties of rubberized asphalt mixtures containing bottom ashes, showing that they have reduced workability and anti-rutting potential, but improved resistance to crack propagation.
JOURNAL OF TESTING AND EVALUATION
(2023)
Article
Green & Sustainable Science & Technology
Michele Lanotte
Summary: By comparing a machine learning-based approach with a traditional method, the study found that the machine learning method is more suitable for predicting the viscosity of rubberized bitumen. The MGGP algorithm provided a practical equation for initial tuning of the production process.
Article
Materials Science, Biomaterials
Nouran Farid, Aibobek Seitak, Vincent Chan, Sungmun Lee
Summary: Oxidative stress and overproduction of reactive oxygen species (ROS) play a crucial role in the development of inflammatory bowel diseases. Catalase, which can scavenge hydrogen peroxide (one of the ROS produced in cellular metabolism), has great therapeutic potential. However, its in vivo application for ROS scavenging, especially through oral administration, is currently limited. This study introduced an alginate-based oral drug delivery system that effectively protected catalase from the harsh conditions of the gastrointestinal tract, released it in a pH-dependent manner, and enhanced its absorption via specialized epithelial cells in the small intestine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Gounhanul Shin, Kunn Hadinoto, Sungmun Lee, Jin-Won Park
Summary: The binding affinity between TGF-beta 1 and its receptors was measured using an atomic force microscope. The results showed strong and high binding between them, with little dissociation occurring.
Article
Construction & Building Technology
Luca Desidery, Michele Lanotte
Summary: This study identifies undisclosed crystalline modifiers (UMs) in high polymer-modified binders (HiPMBs) and evaluates their effects on aging phenomena using thermo-chemical and microstructural analyses. The properties of several industrial HiPMBs were evaluated through FT-IR, DSC, FM, and AFM. Results show that calorimetric analysis can readily identify crystalline UMs in most binders. Unlike SBS thermoplastic elastomers, UMs drastically alter the surface microstructure of all binders, causing the disappearance of typical bee-like structures and the emergence of undefined topographies due to interactions with bituminous waxes. The homogeneity of the binders' amorphous phases is not significantly affected. Aging processes affect base bitumen, SBS, and UMs, with the oxidation of bitumen and degradation of SBS evaluated through FT-IR absorption bands and Tg values, and variations in DSC endotherms reflecting the influence on the crystallinity of UMs.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Construction & Building Technology
Haider Ibrahim, Luca Desidery, Michele Lanotte
Summary: Recycled and post-consumers waste plastics have the potential to reduce costs and environmental impacts in asphalt production as polymer modifiers. However, poor bitumen-plastic affinity limits their field applications due to storage stability concerns. A new compound obtained through a chemo-mechanical blending process of devulcanized tire rubber (DVR), waste plastics, and proprietary chemicals improves storage stability by mitigating phase separation, with plasticizers and compatibilizers agents playing a crucial role. The use of DVR-LDPE compound is preferred based on calorimetric and FT-IR characterizations.
INTERNATIONAL JOURNAL OF PAVEMENT RESEARCH AND TECHNOLOGY
(2023)
Article
Construction & Building Technology
Angela Farina, M. Emin Kutay, Michele Lanotte
Summary: The laboratory investigation showed that PSCR-modified mixtures improved thermal cracking resistance but increased rutting susceptibility. However, after five years of service life, no rutting was observed in the field test sections constructed with PSCR-modified HMA, and transverse cracks did not propagate into the PSCR modified material.
INTERNATIONAL JOURNAL OF PAVEMENT RESEARCH AND TECHNOLOGY
(2021)