Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Biotechnology & Applied Microbiology
Fei Xu, Chloe Dawson, Makenzie Lamb, Eva Mueller, Evan Stefanek, Mohsen Akbari, Todd Hoare
Summary: This review addresses the key design criteria and emerging fabrication techniques for translationally-relevant tissue engineering hydrogels. It suggests combining integrated fabrication approaches with successful translational experiences to accelerate the implementation of hydrogels in tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
E. Fornetti, F. De Paolis, C. Fuoco, S. Bernardini, S. M. Giannitelli, A. Rainer, D. Seliktar, F. Magdinier, J. Baldi, R. Biagini, S. Cannata, S. Testa, C. Gargioli
Summary: Three-dimensional (3D) bioprinting has emerged as an optimal tool for tissue engineering. The extrusion-based approach is the most suitable for skeletal muscle tissue engineering due to its ability to produce parallel printing fibers that mimic native tissue architecture. The development of a novel extrusion-based 3D bioprinting system using PEG-Fibrinogen has shown promising results for muscle tissue regeneration, both in vitro and in vivo.
Review
Chemistry, Multidisciplinary
Tamara Birman, Dror Seliktar
Summary: Balancing between chemical and biological functionality is crucial to achieve injectability in hydrogels through specific crosslinking pathways, while maintaining cell compatibility. With the emergence of new injectable biosynthetic materials, their impact in cell therapy and bioprinting is becoming increasingly significant.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Kyoungryong Kim, Subin Jin, Mikyung Shin
Summary: The use of fibrous hydrogel constructs is a promising strategy in tissue engineering due to their ability to mimic the structure of biological tissues. In this study, 3D-printed gelatin methacryloyl (GelMA) hydrogel microfibers were prepared and evaluated for their effect on myoblast differentiation. The presence of random microfibers in the 3D-printed constructs promoted myotubule-like cell formation and anisotropic myotubule formation.
ADVANCED THERAPEUTICS
(2023)
Review
Polymer Science
Jianghong Huang, Jianyi Xiong, Daping Wang, Jun Zhang, Lei Yang, Shuqing Sun, Yujie Liang
Summary: 3D bioprinting is an emerging technology that allows for personalized therapy strategies in regenerative medicine. Over the last two decades, 3D bioprinting hydrogels have significantly advanced the field of cartilage and bone tissue engineering. This review discusses the development and application of 3D bioprinting, as well as future directions for optimization.
Review
Chemistry, Multidisciplinary
Mohamadmahdi Samandari, Jacob Quint, Alejandra Rodriguez-delaRosa, Indranil Sinha, Olivier Pourquie, Ali Tamayol
Summary: Skeletal muscle injuries can lead to mobility limitations and loss of independence, with current treatments showing variable effectiveness. Skeletal muscle tissue engineering and bioprinting are considered promising solutions for reconstructing complex muscle structures and improving functional recovery.
ADVANCED MATERIALS
(2022)
Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Article
Engineering, Manufacturing
Frederik Wulle, Oliver Gorke, Sarah Schmidt, Maximilian Nistler, Gunter E. Tover, Oliver Riedel, Alexanser Verl, Achim Weber, Alexanser Southan
Summary: This paper introduces the potential and advantages of multi-axis 3D printing of hydrogels, and demonstrates through experiments the relative flexibility and water absorbency of 3D printed hydrogels compared to conventional molded hydrogels. The research also highlights related research questions, such as cross-linking chemistry and formulation of hydrogel inks.
ADDITIVE MANUFACTURING
(2022)
Review
Chemistry, Physical
Surendrasingh Y. Sonaye, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Prabaha Sikder
Summary: Volumetric muscle loss (VML) is a severe acute skeletal muscle injury that can be treated using bioprinting methods. In this study, alginate-gelatin composite inks were developed as bioinks, and high-resolution scaffolds for VML treatment were successfully printed using extrusion printing. The optimal parameters for ink formulation and printing were determined, and the printed scaffolds showed potential for treating skeletal muscle defects.
Review
Pharmacology & Pharmacy
Zhen Wang, Yichuan Wang, Jiaqi Yan, Keshi Zhang, Feng Lin, Lei Xiang, Lianfu Deng, Zhenpeng Guan, Wenguo Cui, Hongbo Zhang
Summary: Bone regenerative engineering encompasses scaffold design and applications based on electrospinning and 3D bioprinting technologies, aiming to achieve bone tissue regeneration. Electrospun nanofibrous scaffolds and 3D bioprinted scaffolds play crucial roles in bone regeneration, with promising prospects for future development.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Engineering, Biomedical
Tingting Fan, Shuo Wang, Zongmin Jiang, Shen Ji, Wenhua Cao, Wenli Liu, Yun Ji, Yujing Li, Ng Shyh-Chang, Qi Gu
Summary: 3D printing is an effective method for recreating skeletal muscle tissue, but obtaining large volumes of highly aligned muscle cells remains a challenge. This study demonstrates a feasible strategy for engineering skeletal muscle tissue using sequential culture processes and investigates the role of physical factors in muscle fiber alignment and maturity.
Article
Engineering, Biomedical
Miriam Filippi, Oncay Yasa, Jan Giachino, Reto Graf, Aiste Balciunaite, Lisa Stefani, Robert K. Katzschmann
Summary: Engineered centimeter-scale skeletal muscle tissue is successfully created through bioprinting, allowing the study of muscle pathophysiology and various applications in biomedicine. The design includes perfusable channels for cell survival, mechanical cell stimulation, and myofiber formation. The biohybrid structures coprinted with synthetic elements demonstrate coherent interfaces with living tissue, and the perfusable designs ensure cell viability and enable mechanical tension during matrix remodeling for drug distribution studies.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Andrea Garcia-Lizarribar, Aranzazu Villasante, Jose Antonio Lopez-Martin, Marta Flandez, M. Carmen Soler-Vazquez, Dolors Serra, Laura Herrero, Ana Sagrera, Alejo Efeyan, Josep Samitier
Summary: Acquired muscle diseases, like cancer cachexia, have a negative impact on the prognosis of cancer patients. In vitro models are necessary for studying the underlying mechanisms of these diseases. Extrusion bioprinting is an emerging technique for mimicking the aligned structure of muscle fibers in tissue engineering, but finding a suitable bioink that meets both the rheological and biological requirements of muscle tissue is challenging.
BIOMATERIALS ADVANCES
(2023)
Review
Chemistry, Physical
Dario Presutti, Tarun Agarwal, Atefeh Zarepour, Nehar Celikkin, Sara Hooshmand, Chinmay Nayak, Matineh Ghomi, Ali Zarrabi, Marco Costantini, Birendra Behera, Tapas Kumar Maiti
Summary: Nanozymes, particularly transition metal dichalcogenides (TMDCs)-based nanozymes, show great potential in healthcare and diagnostics due to their unique nano-architecture, tunable semiconducting properties, and compatibility with modification strategies. Recent advances in TMDC-based nanozymes for biosensing and therapeutic applications are discussed, along with synthesis strategies, enzymatic properties, current challenges, and future prospects.
Review
Materials Science, Biomaterials
Alessia Paradiso, Marina Volpi, Chiara Rinoldi, Nehar Celikkin, Nicola Contessi Negrini, Muge Bilgen, Giorgio Dallera, Filippo Pierini, Marco Costantini, Wojciech Swieszkowski, Silvia Fare
Summary: Liver is a vital organ with complex functions and structures. Understanding the molecular mechanisms of liver diseases is crucial for developing effective treatments. In the past decade, 3D human liver in vitro models have emerged as an alternative to animal models, showing promising results in disease research and drug screening.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Martina Marcotulli, Maria Celeste Tirelli, Marina Volpi, Jakub Jaroszewicz, Chiara Scognamiglio, Piotr Kasprzycki, Karol Karnowski, Wojciech Swieszkowski, Giancarlo Ruocco, Marco Costantini, Gianluca Cidonio, Andrea Barbetta
Summary: 3D printing systems have evolved greatly in the last decade, becoming a top technology for academic and industrial applications. However, manufacturing polymeric materials with trabecular porosity and functionally graded architecture through additive platforms remains a technical challenge. This study presents a 3D extrusion printing strategy that enables the construction of high-accuracy polymeric foams with varying density and composition.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Angelique Van Ombergen, Franziska Chalupa-Gantner, Parth Chansoria, Bianca Maria Colosimo, Marco Costantini, Marco Domingos, Alexandre Dufour, Carmelo De Maria, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jos Malda, Alessandro Margarita, Christophe Marquette, Aleksandr Ovsianikov, Emma Petiot, Sophia Read, Leonardo Surdo, Wojciech Swieszkowski, Giovanni Vozzi, Johannes Windisch, Marcy Zenobi-Wong, Michael Gelinsky
Summary: 3D bioprinting has made significant progress in recent years, allowing for the creation of both simple and complex tissue models. The international space agencies recognize the potential of this technology for manufacturing cell and tissue models for space research, particularly in studying the effects of microgravity and cosmic radiation on human tissues. Additionally, bioprinting can produce tissue grafts for clinical applications, which can support medical treatment for astronauts in long-term space missions. The article discusses the opportunities and challenges of using bioprinters in space, particularly in microgravity. While certain steps may be challenging in microgravity, such as handling liquids, this environment can also help overcome issues like cell sedimentation in low-viscosity bioinks. The article hopes to inspire more researchers to explore this field, especially with the availability of bioprinting opportunities at the International Space Station (ISS) in the near future.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Polymer Science
Yagmur Arslan, Alessia Paradiso, Naz Celiktas, Tolga Erdogan, Ozlem Yesil-Celiktas, Wojciech Swieszkowski
Summary: Various patterns exist in nature and can be utilized in tissue engineering through self-organization. The decellularization of plant tissues provides a promising method for tissue regeneration. However, the functionalization and biofabrication strategies of these structures still need improvement.
EUROPEAN POLYMER JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Tarun Agarwal, Irene Chiesa, Marco Costantini, Anna Lopamarda, Maria Celeste Tirelli, Om Prakash Borra, Subhash Venkata Sai Varshapally, Yanamadala Ashwin Vijay Kumar, G. Koteswara Reddy, Carmelo De Maria, Lijie Grace Zhang, Tapas Kumar Maiti
Summary: Tissue engineering research has been greatly improved by the introduction of bioprinting technologies, which allow the development of customized biomaterial inks with excellent printability and cell-promoting abilities. This review focuses on chitosan-based materials as suitable inks for 3D/4D bioprinting, and their applications in drug delivery platforms and tissue engineering constructs. Strategies to enhance the mechanical and biological performances of chitosan biomaterials are also highlighted.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Francesco Nalin, Maria Celeste Tirelli, Piotr Garstecki, Witold Postek, Marco Costantini
Summary: tuna-step is a novel microfluidic module based on step emulsification that allows for reliable generation of droplets of different sizes. By incorporating a tunable nozzle and a new surface modification method, we achieved variation in droplet volume and produced functionally graded soft materials.
Meeting Abstract
Cell & Tissue Engineering
Martina Marcotulli, Maria Celeste Tirelli, Arianna Iacomino, Marco Costantini, Andrea Barbetta, Gianluca Cidonio
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Alessia Paradiso, Marina Volpi, Ewa Walejewska, Diana Martinez Guerrero, Maciej Lojkowski, Marco Costantini, Wojciech Swieszkowski
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Pasquale Posabella, Marcin Heljaki, Marco Costantini, Wojciech Swiqszkowskil
TISSUE ENGINEERING PART A
(2023)
Review
Materials Science, Biomaterials
Ganma, Tarun Agarwal, Marco Costantini, Sampriti Pal, Arun Kumar
Summary: The degree of oxygenation is a crucial factor in wound healing, affecting various processes and ultimately determining the success of tissue recovery. This article provides an overview of the role of oxygen and the different oxygenation therapies used in healing dermal wounds. Additionally, it critically assesses the challenges and opportunities for delivering oxygen effectively and safely at the wound site.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Meeting Abstract
Cell & Tissue Engineering
Marco Costantini, Nehar Celikkin, Dario Presutti, Cesare Gargioli
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Jan Guzowski, Katarzyna Rojek, Marco Costantini, Monika Cwiklinska, Robert Buda
TISSUE ENGINEERING PART A
(2022)