Article
Cell Biology
Rosario Sanchez-Gonzalez, Christina Koupourtidou, Tjasa Lepko, Alessandro Zambusi, Klara Tereza Novoselc, Tamara Durovic, Sven Aschenbroich, Veronika Schwarz, Christopher T. Breunig, Hans Straka, Hagen B. Huttner, Martin Irmler, Johannes Beckers, Wolfgang Wurst, Andreas Zwergal, Tamas Schauer, Tobias Straub, Tim Czopka, Dietrich Truembach, Magdalena Goetz, Stefan H. Stricker, Jovica Ninkovic
Summary: The influx of cerebrospinal fluid after brain injury simultaneously activates toll-like receptor 2 (Tlr2) and chemokine receptor 3 (Cxcr3) pathways, leading to increased OPC proliferation and exacerbated glial reactivity. Interference with these pathways alleviated reactive gliosis, increased new neuron recruitment, and improved tissue restoration after injury.
Article
Developmental Biology
Adam J. Isabella, Jason A. Stonick, Julien Dubrulle, Cecilia B. Moens
Summary: Regeneration after peripheral nerve damage requires axons to grow to correct target tissues in a process called target-specific regeneration. Research on zebrafish vagus motor nerve reveals strong target-specific regrowth capabilities, with regeneration relying on neurons' intrinsic memory of their position and pre-existing innervation.
Article
Neurosciences
Kadidia Pemba Adula, Matthew Shorey, Vasudha Chauhan, Khaled Nassman, Shu-Fan Chen, Melissa M. Rolls, Alvaro Sagasti
Summary: DLK and LZK play different roles in zebrafish, promoting axon regeneration in motor neurons while inhibiting excessive growth in sensory neurons.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Wen-Yuan Shen, Xuan-Hao Fu, Jun Cai, Wen-Chang Li, Bao-You Fan, Yi-Lin Pang, Chen-Xi Zhao, Muhtidir Abula, Xiao-Hong Kong, Xue Yao, Shi-Qing Feng
Summary: Zebrafish are an effective model for studying recovery after spinal cord injury. This study identified differentially expressed genes involved in tissue bridging and axon regeneration during the subacute phase of recovery. The up-regulated gene clasp2 functions similarly to microtubule-associated protein, while the down-regulated gene h1m controls endogenous stem cell differentiation after spinal cord injury. These findings provide new potential therapeutic targets for spinal cord injury repair.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Zulvikar Syambani Ulhaq, William Ka Fai Tse
Summary: Unlike mammals, zebrafish have the ability to regenerate various organs. Analyzing the proteome profiles of regenerated zebrafish tissues revealed the importance of proteins related to cytoskeleton, protein synthesis and degradation, cell cycle control, and energy metabolism. However, proteins responsible for inflammation and immune response, crucial for initiating the regeneration process, were less frequently detected. This highlights the need for more sensitive proteomic analysis to unravel the mechanism.
Review
Cell Biology
Vasiliki Tsata, Daniel Wehner
Summary: The capacity for long-distance axon regeneration and functional recovery after spinal cord injury is poor in mammals but remarkable in some vertebrates, like fish and salamanders. The cellular and molecular basis of this difference is starting to emerge, including the identification of target cells reacting to injury and cues directing pro-regenerative responses. Zebrafish is one of the most understood models in terms of successful spinal cord regeneration, with neuron-intrinsic and extrinsic factors playing pivotal roles in axon regeneration and function recovery.
Article
Cell Biology
Ashley C. Kramer, Katherine Gurdziel, Ryan Thummel
Summary: The study revealed that adult zebrafish have the ability to completely regenerate their retinas through the re-entry of Muller glia into the cell cycle and differentiation into new photoreceptors after intense light exposure. Additionally, the research identified two peaks of MG gliosis, a distinct transcriptional shift between 5- and 10-days post lesion, and different patterns of transcriptional recovery of photoreceptor opsins at 28 days post lesion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yu Xia, Sierra Duca, Bjorn Perder, Friederike Dundar, Paul Zumbo, Miaoyan Qiu, Jun Yao, Yingxi Cao, Michael R. M. Harrison, Lior Zangi, Doron Betel, Jingli Cao
Summary: This study identified a transiently activated epicardial progenitor cell (aEPC) subpopulation marked by ptx3a and col12a1b expression in adult zebrafish hearts. Upon cardiac injury, aEPCs emerged from the epithelial epicardium, underwent epithelial-mesenchymal transition (EMT), and differentiated into different cell types, crucial for heart regeneration.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Eva E. Stefanova, Angela L. Scott
Summary: In recent decades, significant progress has been made in understanding the pathophysiology of spinal cord injury. Although conventional mammalian models have contributed to advances, the regeneration of cells and axons following spinal cord injury remains a challenge. Non-mammalian regenerative species offer a unique opportunity to study the pro-regenerative cues and spinal cord microenvironment. Purinergic signaling, which is conserved across species, has been implicated in secondary processes related to spinal cord injury, including cell death, inflammation, reactive gliosis, and neural regeneration.
NEURAL REGENERATION RESEARCH
(2023)
Review
Cell Biology
Eva E. Stefanova, Angela L. Scott
Summary: The scientific community has made significant progress in understanding the complex pathophysiology of spinal cord injury, but cellular and axonal regeneration still remains a challenge. Therefore, studying regenerative species that are not mammals can provide insights into promoting regeneration in the spinal cord. The purinergic signaling system has been hypothesized to play a role in spinal cord injury, affecting secondary processes such as cell death, inflammation, gliosis, and neural regeneration. Comparing the roles of purinergic signaling in different species can give us important information about conditions that lead to successful recovery.
NEURAL REGENERATION RESEARCH
(2022)
Article
Cell Biology
Sean Mccracken, Michael J. Fitzpatrick, Allison L. Hall, Zelun Wang, Daniel Kerschensteiner, Josh L. Morgan, Philip R. Williams
Summary: Retinal ganglion cell (RGC) degeneration, caused by axon degeneration, is the main reason for vision loss in blinding conditions. Axonal Ca2+ elevations from optic nerve injury do not reach RGC somas, and baseline Ca2+ levels of RGCs predict their survival after axon injury.
Article
Cell Biology
Yuki Shimizu, Takashi Kawasaki
Summary: The study compared the neural stem cell responses and regenerative capacity in the optic tectum of adult medaka and zebrafish after injury, finding limited neuronal generation and scar formation in injured medaka. This suggests that adult medaka brain has low regenerative capacity and represents an attractive model for investigating critical factors for brain regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Chun Cui, Lin-Fang Wang, Shu-Bing Huang, Peng Zhao, Yong-Quan Chen, Yi-Bo Wu, Chen-Meng Qiao, Wei-Jiang Zhao, Yan-Qin Shen
Summary: The study found that the expression of NPY decreased in adult zebrafish spinal cord after injury, and inhibiting NPY expression impeded descend axon regeneration and locomotor recovery. This suggests that NPY in motoneurons may promote recovery by regulating motoneuron proliferation through activation of NPY1R.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Pharmacology & Pharmacy
Laura Gence, Danielle Fernezelian, Matthieu Bringart, Bryan Veeren, Armelle Christophe, Francois Brion, Olivier Meilhac, Jean-Loup Bascands, Nicolas Diotel
Summary: This study investigates the biological properties of the traditional medicinal plant Hypericum lanceolatum Lam., revealing its antioxidant, pro-regenerative, anti-lipid accumulation, and pro-neurogenic effects. The results support the potential use of this plant as an alternative treatment for metabolic disorders.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Hui Xia, Huimin Chen, Xue Cheng, Mingzhu Yin, Xiaowei Yao, Jun Ma, Mengzhen Huang, Gang Chen, Hongtao Liu
Summary: Zebrafish is a low-cost and genetically tractable vertebrate model that can be used to investigate the interaction between gut microbiota and host. It has features such as high fecundity, external fertilization, and early optical transparency, making it a valuable tool for addressing research questions not easily addressed in other animal models.
MOLECULAR MEDICINE
(2022)