Article
Surgery
Takayuki Yamamoto, Srilakshmi Atthota, Divyansh Agarwal, Kerry Crisalli, Malcolm Macconmara, Tsukasa Nakamura, Richard Teo, Leigh Anne Dageforde, Shoko Kimura, Nahel Elias, Heidi Yeh, Adel Bozorgzadeh, Tatsuo Kawai, James F. Markmann
Summary: This study compares the outcomes of liver allografts preserved using portable normothermic machine perfusion (NMP) and ischemic cold storage (ICS) in liver transplantation procedures. It concludes that NMP can significantly reduce the incidence of early allograft dysfunction, reperfusion syndrome, and ischemic cholangiopathy after donation after circulatory death (DCD) liver transplantation.
Article
Medicine, General & Internal
R. Van Rijn, I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. Vanlander, J. de Jonge, R. J. Porte
Summary: This study demonstrated that hypothermic oxygenated machine perfusion can reduce the risk of nonanastomotic biliary strictures following liver transplantation from donors after circulatory death, with lower rates of complications compared to conventional static cold storage.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Medicine, General & Internal
Andras T. Meszaros, Julia Hofmann, Madita L. Buch, Benno Cardini, Theresia Dunzendorfer-Matt, Florian Nardin, Michael J. Blumer, Margot Fodor, Martin Hermann, Bettina Zelger, Giorgi Otarashvili, Melanie Schartner, Annemarie Weissenbacher, Rupert Oberhuber, Thomas Resch, Jakob Troppmair, Dietmar Oefner, Heinz Zoller, Herbert Tilg, Erich Gnaiger, Theresa Hautz, Stefan Schneeberger
Summary: Detailed analysis of mitochondrial function and tissue damage can predict the clinical outcome of liver transplantation.
Article
Biotechnology & Applied Microbiology
Pierre-Alain Clavien, Philipp Dutkowski, Matteo Mueller, Dilmurodjon Eshmuminov, Lucia Bautista Borrego, Achim Weber, Beat Muellhaupt, Richard X. Sousa Da Silva, Brian R. Burg, Philipp Rudolf von Rohr, Martin J. Schuler, Dustin Becker, Max Hefti, Mark W. Tibbitt
Summary: A human liver has been successfully transplanted using ex situ normothermic machine perfusion, which extends the time window for evaluating the viability of donor organs and transforms the urgency of the surgery to an elective procedure.
NATURE BIOTECHNOLOGY
(2022)
Article
Surgery
Cristiano Quintini, Luca Del Prete, Andrea Simioni, Laurent Del Angel, Teresa Diago Uso, Giuseppe D'Amico, Koji Hashimoto, Federico Aucejo, Masato Fujiki, Bijan Eghtesad, Kazunari Sasaki, Choon Hyuck David Kwon, Jacek Cywinski, Ana Bennett, Mary Bilancini, Charles Miller, Qiang Liu
Summary: This study demonstrated the successful transplantation of 71.5% of discarded livers after ex vivo normothermic machine perfusion and assessment using an institutionally built device. The findings challenge the current viability criteria and call for standardization of viability marker collection.
Article
Surgery
Zoltan Czigany, Johann Pratschke, Jiri Fronek, Markus Guba, Wenzel Schoening, Dimitri Aristotle Raptis, Joachim Andrassy, Matthijs Kramer, Pavel Strnad, Rene Hany Tolba, Wenjia Liu, Theresa Keller, Hannah Miller, Sandra Pavicevic, Deniz Uluk, Matej Kocik, Isabella Lurje, Christian Trautwein, Arianeb Mehrabi, Irinel Popescu, Florian Wolfgang Rudolf Vondran, Cynthia Ju, Frank Tacke, Ulf Peter Neumann, Georg Lurje
Summary: This study demonstrates that HOPE significantly reduces early allograft injury and improves post-transplant outcomes in ECD-DBD liver transplantation compared to SCS.
Article
Immunology
Andy Chao Hsuan Lee, Arianna Edobor, Maria Lysandrou, Vikranth Mirle, Amir Sadek, Laura Johnston, Ryan Piech, Rebecca Rose, John Hart, Beth Amundsen, Martin Jendrisak, James Michael Millis, Jessica Donington, Maria Lucia Madariaga, Rolf N. Barth, Diego di Sabato, Kumaran Shanmugarajah, John Fung
Summary: NMP can alter the immune profile of donor livers, providing a potential strategy for immune modulation prior to transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Melanie Kruger, Alicia Ruppelt, Benjamin Kappler, Elke Van Soest, Roos Anne Samsom, Guy C. M. Grinwis, Niels Geijsen, J. Bernd Helms, Marco Stijnen, Linda M. Kock, Marco Rasponi, Hans S. Kooistra, Bart Spee
Summary: This study established a feasible large animal model for studying liver diseases by collecting porcine livers in a slaughterhouse and perfusing them in vitro. The model allows for the investigation of metabolic and toxic liver disorders in the early stages and the observation of biochemical markers and functional impairment of liver damage. It provides a reliable alternative to experimental animals for studying liver diseases and potential treatments.
BIOENGINEERING-BASEL
(2022)
Review
Gastroenterology & Hepatology
Jiahao Li, Haoyang Lu, Jian Zhang, Yixuan Li, Qiang Zhao
Summary: This review summarizes the criteria, indicators, and methods used to evaluate liver viability during normothermic machine perfusion (NMP) in liver transplantation. It discusses the analysis of liver shape, appearance, and hemodynamics at a macroscopic level and biochemical analysis of liver injury markers, liver and bile duct function indicators, and other relevant indicators. It also introduces novel methods for assessing liver viability and provides suggestions for future clinical practice considering the limitations of evaluating liver viability during NMP.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2023)
Review
Medicine, General & Internal
Joseph Mugaanyi, Lei Dai, Changjiang Lu, Shuqi Mao, Jing Huang, Caide Lu
Summary: Machine perfusion, particularly HOPE/D-HOPE and NMP, shows promise as alternatives to static cold storage for the preservation of donor livers. These methods improve graft and patient survival rates and reduce the incidence of biliary complications. They have the potential to address the gap between demand and availability of donor livers by enabling the utilization of marginal livers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, General & Internal
Fabio Melandro, Riccardo De Carlis, Francesco Torri, Andrea Lauterio, Paolo De Simone, Luciano De Carlis, Davide Ghinolfi
Summary: This review summarizes the current literature on viability criteria during normothermic and hypothermic machine perfusion, and discusses the future steps and evolution of these technologies.
MEDICINA-LITHUANIA
(2022)
Article
Engineering, Biomedical
M. Magbagbeola, Z. L. Rai, K. Doyle, L. Lindenroth, G. Dwyer, A. Gander, A. Stilli, B. R. Davidson, D. Stoyanov
Summary: This paper presents a low-cost organ perfusion machine that is modular and versatile, allowing for the addition of specific sensors for different research applications. The machine was assessed for perfusion efficacy, functionality, and viability using various measurements and assays. The results show that the system is capable of successfully perfusing porcine livers for up to three hours, maintaining their viability and functionality.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2023)
Article
Surgery
Qiang Liu, Luca Del Prete, Khaled Ali, Patrick Grady, Mary Bilancini, John Etterling, Giuseppe D'Amico, Teresa Diago Uso, Koji Hashimoto, Federico Aucejo, Masato Fujiki, Bijan Eghtesad, Kazunari Sasaki, Choon Hyuck David Kwon, Sulemon Chaudhry, Junshi Doi, Alejandro Pita, Brandon New, Ana Bennett, Jacek Cywinski, Charles Miller, Cristiano Quintini
Summary: The purpose of this study was to assess the safety and feasibility of sequential hypothermic oxygenated perfusion and normothermic machine perfusion and the potential benefits of graft viability preservation and assessment before liver transplantation. 17 expanded criteria donor livers underwent sequential hypothermic oxygenated perfusion and normothermic machine perfusion. The results showed that sequential hypothermic oxygenated perfusion and normothermic machine perfusion preservation is safe and feasible and has the potential benefits of preserving and evaluating expanded criteria donor livers.
Article
Surgery
William C. Chapman, Andrew S. Barbas, Anthony M. D'Alessandro, Rodrigo Vianna, Chandrashekhar A. Kubal, Peter Abt, Christopher Sonnenday, Rolf Barth, Josue Alvarez-Casas, Hasan Yersiz, Devin Eckhoff, Robert Cannon, Yuri Genyk, Linda Sher, Andrew Singer, Sandy Feng, Garrett Roll, Ari Cohen, Maria B. Doyle, Debra L. Sudan, David Al-Adra, Adeel Khan, Vijay Subramanian, Nader Abraham, Kim Olthoff, Akin Tekin, Lynn Berg, Constantin Coussios, Chris Morris, Lucy Randle, Peter Friend, Stuart J. Knechtle
Summary: This study compared the effects of conventional low-temperature storage and storage at physiological body temperature on liver transplantation. The results showed that normothermic machine perfusion (NMP) did not significantly reduce early posttransplant liver injury and function, but it may have greater benefits for high-risk donor livers.
Review
Medicine, General & Internal
Charles W. G. Risbey, Carlo Pulitano
Summary: Liver transplantation is a life-saving procedure for patients with end-stage liver disease, but the shortage of donors prevents many patients from receiving transplants. This paper investigates the clinical progress of ex vivo normothermic machine perfusion (NMP) as an alternative preservation technique for liver transplantation in humans.
JOURNAL OF CLINICAL MEDICINE
(2023)
Letter
Surgery
Shaheed Merani, Marian Urban, John Y. Um, Alexander Maskin, Luciano M. Vargas, Anthony W. Castleberry, David F. Mercer, Wendy Grant, Alan N. Langnas
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Surgery
Marian Urban, Anthony W. Castleberry, Nicholas W. Markin, Megan M. Chacon, Heather M. Strah, John Y. Um, David Berkheim, Shaheed Merani, Aleem Siddique
Summary: Lung transplantation from donors after circulatory death is now seen as a viable alternative to traditional brain death donation. The emergence of TA-NRP as a technique for cardiac allograft procurement from circulatory death cases allows for in situ organ assessment, potentially impacting lung allograft quality. However, successful cases of transplantation using this method have been reported.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Cardiac & Cardiovascular Systems
Marian Urban, Anthony W. Castleberry, Kim F. Duncan, Nicholas W. Markin, M. Megan Chacon, Aleem Siddique, Shaheed Merani, Ryan Robertson, Lance Fristoe, John Y. Um
Summary: Donation after circulatory death is a viable option for expanding the pool of cardiac organ donors. This article describes the surgical technique and circuit configuration for in-situ organ reperfusion using portable venoarterial extracorporeal membrane oxygenation.
ANNALS OF THORACIC SURGERY
(2022)
Article
Anatomy & Morphology
Travis L. McCumber, Justin L. Mott, Shaheed Merani, Fedja A. Rochling
Summary: This report examines the integration of teaching anatomical science with clinical implications in minimally invasive surgery. The authors implemented laparoscopic simulation during undergraduate medical education and found that it improved students' learning of anatomical structures and their preparation for clinical rotations.
Article
Surgery
Jon S. Thompson, Fedja A. Rochling, Elizabeth Lyden, Shaheed Merani, Luciano M. Vargas, Wendy J. Grant, Alan N. Langnas, David F. Mercer
Summary: Previous cholecystectomy in SBS patients does not affect the nutritional prognosis of SBS, regardless of the intestinal remnant length and anatomy type.
AMERICAN JOURNAL OF SURGERY
(2022)
Article
Surgery
Eva Breuer, Matteo Mueller, Majella B. Doyle, Liu Yang, Sarwa Darwish Murad, Imran J. Anwar, Shaheed Merani, Ashley Limkemann, Heithem Jeddou, Steven C. Kim, Victor Lopez-Lopez, Ahmed Nassar, Frederik J. H. Hoogwater, Eric Vibert, Michelle L. De Oliveira, Daniel Cherqui, Robert J. Porte, Joseph F. Magliocca, Lutz Fischer, Constantino Fondevila, Krzysztof Zieniewicz, Pablo Ramirez, David P. Foley, Karim Boudjema, Austin D. Schenk, Alan N. Langnas, Stuart Knechtle, Wojciech G. Polak, C. Burcin Taner, William C. Chapman, Charles B. Rosen, Gregory J. Gores, Philipp Dutkowski, Julie K. Heimbach, Pierre-Alain Clavien
Summary: This multicenter benchmark study demonstrates that liver transplantation offers excellent outcomes with superior oncological results in early stage perihilar cholangiocarcinoma patients, even in candidates for surgery. This provocative observation should lead to a change in available therapeutic algorithms for perihilar cholangiocarcinoma.
Editorial Material
Oncology
Mehmet Sitki Copur, Soe Min Tun, Luciano Vargas, Shaheed Merani, Whitney Wedel, Randy Duckert, Adam Horn, Nicholas Lintel, Daniel Herold, Swathi Lavudi
CLINICAL COLORECTAL CANCER
(2023)
Article
Surgery
Daniel J. Cloonan, Madeline R. Cloonan, Scott G. Westphal, Leigh Anne Dageforde, Shaheed Merani
Summary: Patients with acute liver failure (ALF) who have kidney injury (KI) at the time of liver transplantation (LT) are at risk for persistent post-transplant kidney injury, lower survival rate, and increased need for kidney transplantation (KT).
CLINICAL TRANSPLANTATION
(2023)
Meeting Abstract
Surgery
David Reich, Andrea Schlegel, Michael Rizzari, Koji Hashimoto, Sander Florman, Michael De Vera, David Foley, Shennen Mao, William Chapman, Garrett Roll, Antonio Pinna, Ashish Saharia, Shaheed Merani, Humberto Bohorquez, Mohamed Akoad, Shekhar Kubal, Sophoclis Alexopoulos
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Pediatrics
Kelsey Klein, Megan Keck, Eric Langewisch, Shaheed Merani, Kelley Hitchman, Mary Leick
Summary: This study aimed to assess the appearance, clearance, specificity, and impact of anti-HLA donor-specific antibodies (DSA) in intestinal/multivisceral (MV) transplant and the value of serial monitoring. The results showed that DSA may lead to increased biopsy-proven acute rejection (BPAR) or shorter time to BPAR, although the differences were not statistically significant. DSA were usually identified within the first month after transplant and could serve as early rejection biomarkers. The study also identified a wide range of HLA alleles targeted by DSA in intestinal/MV patients.
PEDIATRIC TRANSPLANTATION
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Nathalie Khoury, Tomoki Sempokuya, Chaeli Fortina, Abbey Christensen, Katherine Brodahl, Robyn Thorson, Reagan Huber, Marco A. Olivera-Martinez, Timothy M. McCashland, Alan N. Langnas, Shaheed Merani
Meeting Abstract
Oncology
Erin Oeltjen, Makalya Schissel, Kelly Erickson, Shaheed Merani, James Ford
PEDIATRIC BLOOD & CANCER
(2022)
Meeting Abstract
Surgery
Shaheed Merani, Marian Urban, John Um, Alexander Maskin, Luciano Vargas, Anthony Castleberry, David Mercer, Wendy Grant, Alan Langnas
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Surgery
Jacob Moore, Melaine Kaiser, Arika Hoff Man, Alexander Maskin, Clifford Miles, Scott Westphal, Eric Langewisch, Shaheed Merani
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)