Article
Biochemistry & Molecular Biology
Krisztina Nikovics, Cedric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier
Summary: Understanding bone homeostasis and regeneration is of great clinical and scientific importance for the development of bone therapy. Macrophages play a crucial role in bone regeneration during healing. In situ hybridization and hybridization chain reaction can be used to explore gene expression in bone tissues.
Article
Environmental Sciences
Varsha Dogra, Gurpreet Kaur, Rajeev Kumar, Sandeep Kumar
Summary: In this study, ruthenium and ruthenium oxide nanoparticles derived from different metallosurfactants were synthesized and their nanotoxicity towards Saccharomyces cerevisiae was investigated. Results showed that RuCTAC had the most toxic effect on yeast cells, with RuCTAC and RuHEXA causing the most damage to yeast genomic DNA.
Article
Engineering, Multidisciplinary
Rana Abbas Azeez, Firas Khaleel Ismael Al-Zuhairi
Summary: Bioremediation is an efficient technique for environmental pollution control. In this study, yeast cells were immobilized on the surface of magnetic nanoparticles to remove dye from aqueous solution. The experiments determined that the optimal conditions for removal efficiency were pH 6.5, 50 mg/l dye concentration, 1.5 g/l biosorbent dosage, 110 rpm agitation, and 35 degrees C temperature. The adsorption isotherm analysis indicated that the biosorption process followed the Freundlich model, and it was exothermic and spontaneous.
ALEXANDRIA ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Rohan Dandage, Caroline M. Berger, Isabelle Gagnon-Arsenault, Kyung-Mee Moon, Richard Greg Stacey, Leonard J. Foster, Christian R. Landry
Summary: The study investigated protein-protein interactions in hybrids between yeast species, finding that most interactions are similar to those of the parents but with some altered instances. It suggests that the occurrence of chimeric protein complexes is frequent, potentially due to incompatibilities or imbalances between parental proteomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Marine & Freshwater Biology
Zeyu Jia, Yijing Dong, Heng Xu, Fengping Wang
Summary: The study optimized the HCR-FISH protocol to address the issues of low signal intensity and false-positive signals, successfully identifying HCR initiator/amplifier pairs with high efficiency and specificity. Through the optimization of sample pretreatment methods, HCR-FISH protocol combinations, and image processing, false positive signals were reduced.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zachari Turgeon, Thomas Sierocinski, Cedric A. Brimacombe, Yiqiong Jin, Brittany Goldhawke, Jessica M. Swanson, John Husnik, Matthew S. Dahabieh
Summary: Lager beer is produced by a natural hybrid yeast, and lack of strain diversity has driven research to create new lager hybrids with unique genomic compositions. A novel breeding strategy has successfully produced de novo lager hybrids with fermentation characteristics similar to commercial strains but without undesirable brewing attributes of wild strains. These novel lager hybrids are immediately suitable for industrial lager beer production and may lead to the creation of additional diverse commercial lager strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Huo Xu, Min Lin, Yanhui Zheng, Xiaojun Fang, Xinmei Huang, Qi Huang, Jiawei Xu, Wei Duan, Juan Wei, Lee Jia
Summary: This study designed a multifunctional linear DNA nanostructure (MLN) as a carrier of molecular beacons (MB-21) for detecting and intracellular imaging miRNA-21. The MLN-MB exhibited a detection limit of 320 pM for miRNA-21 and high specificity against other miRNAs. With its outstanding stability, dual recognition, and biocompatibility, MLN-MB holds great potential for early cancer diagnosis.
Article
Biochemistry & Molecular Biology
Jinfan Wang, Byung-Sik Shin, Carlos Alvarado, Joo-Ran Kim, Jonathan Bohlen, Thomas E. Dever, Joseph D. Puglisi
Summary: Through real-time single-molecule fluorescence spectroscopy, it has been found that the eukaryotic 43S preinitiation complex engages with mRNA through a slow, ATP-dependent process and proceeds to scan rapidly and directionally along the 5' untranslated region (5' UTR). Scanning ribosomes can traverse RNA secondary structures, but hairpin structures near the start codons drive them backward in the 5' direction, requiring rescanning.
Article
Chemistry, Analytical
Yu-ki Tanaka, Shunsuke Shimazaki, Yasunori Fukumoto, Yasumitsu Ogra
Summary: A rapid and precise quantification method for a histidine-tagged recombinant protein produced in Escherichia coli (E. coli) has been developed using single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). This method allows for the determination of protein quantity based on the signal of nickel or cobalt ions bound to the His-tag, without the need for extraction and purification processes.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mark Boltengagen, Daan Verhagen, Michael Roland Wolff, Elisa Oberbeckmann, Matthias Hanke, Ulrich Gerland, Philipp Korber, Felix Mueller-Planitz
Summary: In this study, the organization of nucleosomes in Saccharomyces cerevisiae was investigated using the Fiber-Seq technique. The results revealed deviations from the stereotypical nucleosome organization, including long linker DNAs between nucleosomes, missing nucleosomes in gene bodies, cell-to-cell heterogeneity in nucleosome occupancy, heterogeneous phasing of arrays, and irregular nucleosome spacing. These findings provide valuable information for understanding the chromatin structure and function.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Laura Vanderwaeren, Ruveyda Dok, Karin Voordeckers, Sandra Nuyts, Kevin J. Verstrepen
Summary: Yeast is an important enzyme source for bread and beer production and is an ideal model organism for studying eukaryotic cell biology and genetics. This review discusses the characteristics that make yeast such a widely used model organism, with a focus on the DNA damage response pathway as an example of its contribution to elucidating highly conserved biological processes. Differences in the DNA damage response of yeast and humans are also highlighted, along with the challenges of using yeast as a model system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Zhen Zhu, Yingying Wang, Ruobo Peng, Pan Chen, Yangye Geng, Bailiang He, Shuiping Ouyang, Ke Zheng, Yimin Fan, Dejing Pan, Nan Jin, Fabian Rudolf, Andreas Hierlemann
Summary: The MiSCA microfluidic single-cell array allows for immobilizing yeast cells in a precisely controlled microenvironment and conducting comparative culturing under the influence of different media and compounds. This system enables parallel sets of experiments and control experiments, as well as comparative experiments under well-defined laminar-perfusion conditions.
Article
Biochemistry & Molecular Biology
Jessica B. Lee, Leandra M. Caywood, Jennifer Y. Lo, Nicholas Levering, Albert J. Keung
Summary: Biological information can be encoded within the dynamics of signaling components, with experiments showing that gene expression and filtering behaviors can be altered by tuning the pulse frequency, amplitude, and pulse width of light-regulating epigenome editor binding. The study demonstrates a quantification of the limit to the amount of information that can be reliably transferred across a single promoter, as well as the ability of chromatin state to tune mutual information and alter the input-output transfer function of the promoter, unlocking the information-rich content of eukaryotic gene regulation.
Article
Chemistry, Analytical
Zihan Song, Yun Zhou, Minzhe Shen, Dong Zhao, Haihong Hu, Su Zeng, Lianli Sun, Sheng Cai
Summary: In this study, a simple enzyme-free method for detecting and imaging MUC1 was developed. It was successfully used to detect MUC1 in specific cells and perform in situ imaging.
Article
Chemistry, Physical
Min Seok Lee, Hwi Hyun, Inwon Park, Sungho Kim, Dong-Hyun Jang, Seonghye Kim, Jae-Kyeong Im, Hajin Kim, Jae Hyuk Lee, Taejoon Kwon, Joo H. Kang
Summary: This study developed a novel technology called mu FISH, which uses mu FISH probes based on 16S rRNA sequences to quantitatively identify and detect bacteria in blood samples. It is a more sensitive method compared to traditional bacterial detection methods.
Article
Materials Science, Multidisciplinary
J. Fliegans, C. Rado, R. Soulas, L. Guetaz, O. Tosoni, N. M. Dempsey, G. Delette
Summary: Grain boundary diffusion process is widely used in Nd-Fe-B magnets to increase coercivity but can result in a coercivity gradient within the magnet. Using Dy63Co37 intermetallic compound diffusion, it was found that the shape of the demagnetization curve in the magnet is influenced by composition profiles.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Gabriel Gomez Eslava, Bahar Fayyazi, Konstantin Skokov, Yurii Skourski, Denis Gorbunov, Oliver Gutfleisch, Nora M. Dempsey, Dominique Givord
Summary: Anisotropy constants were obtained by analyzing single crystal magnetization curves, taking into account the anisotropy of the 3d transition metal sublattice and the molecular exchange field coupling between rare-earth and transition metal sublattices. The results demonstrated the influence of Co and/or Ce doping on magnetocrystalline anisotropy in Nd2Fe14B single crystals, indicating that co-doping with Ce and Co may improve the performance of NdFeB-based magnets by enhancing intrinsic properties.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2021)
Article
Chemistry, Physical
Gabriel Gomez Eslava, Masaaki Ito, Claire Colin, Masao Yano, Tetsuya Shoji, Akira Kato, Emmanuelle Suard, Nora M. Dempsey, Dominique Givord
Summary: The study revealed that atom substitution in Nd-2(Fe1-xCox)(14)B, Y-2(Fe1-xCox)(14)B and Ce-2(Fe1-xCox)(14)B compounds shows some regularity, depending on the ratio of Co to Fe content. Steric hindrance primarily determines atom substitution, and enthalpy of mixing must also be considered for a more accurate description of Co preferential occupancies.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Materials Science, Multidisciplinary
T. Nguyen Van, I de Moraes, N. M. Dempsey, C. Champeaux, F. Dumas-Bouchiat
Summary: Nd-Fe-B thin films were successfully developed using Pulsed Laser Deposition (PLD) onto silicon substrates. Among different target compositions tested, the thin films made from a target with Nd/Fe = 0.45 exhibited the best hard magnetic properties due to film texturation and partial decoupling of the hard magnetic grains.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2021)
Editorial Material
Materials Science, Multidisciplinary
Nora M. Dempsey, Yuepeng Zhang
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2021)
Article
Physics, Applied
Hawa Latiff, Gabriel Gomez Eslava, Thibaut Devillers, Hideto Yanagihara, Nora M. Dempsey, Dominique Givord
Summary: The coercivity of cubic and tetragonally distorted Co_xCu_1-xFe_2O_4 particles was analyzed using a global model, with activation volumes and microstructure-related parameters obtained. High-resolution transmission electron microscopy images showed that nanograins in the tetragonal sample are connected through a thin amorphous layer, explaining the negative N-eff value and M-r/M-s > 0.5.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Materials Science, Multidisciplinary
Semih Ener, Konstantin P. Skokov, Dhanalakshmi Palanisamy, Thibaut Devillers, Johann Fischbacher, Gabriel Gomez Eslava, Fernando Maccari, Lukas Schafer, Leopold V. B. Diop, Iliya Radulov, Baptiste Gault, Gino Hrkac, Nora M. Dempsey, Thomas Schrefl, Dierk Raabe, Oliver Gutfleisch
Summary: Nd2Fe14B-type materials dominate the high-performance permanent magnet market due to their high energy product around room temperature. However, compounds with a ThMn12-type structure, such as SmFe11 Ti, show comparable or even superior intrinsic properties. The performance of ThMn12-based bulk sintered magnets is limited by the formation of twins inside micro-crystalline grains, impacting coercivity and remanent magnetization.
Article
Biology
David Sala, Vincent Grossi, Helene Agogue, Christophe Leboulanger, Didier Jezequel, Gerard Sarazin, Ingrid Antheaume, Cecile Bernard, Magali Ader, Mylene Hugoni
Summary: Studies on microbial communities in aphotic euxinic waters are limited compared to those in euxinic photic zones. Lake Dziani Dzaha, a tropical, saline, alkaline crater lake, has been identified as a modern analog of Proterozoic Oceans. The presence of aphotic haloclines and euxinia strongly influences the molecular composition of organic matter and microbial diversity in the lake.
Article
Chemistry, Inorganic & Nuclear
Marie Frenea-Robin, Julien Marchalot
Summary: Magnetic cell separation is a key method for isolating target cell populations from biological suspensions. It has a wide range of applications in biomedicine, environmental applications, and biological research. Currently, there are many commercially available separation instruments and reagents, but there is still a need for improved selectivity, yield, sensitivity, and cost-effectiveness in the separation process.
Review
Environmental Sciences
Zelia Bontemps, Lise Alonso, Thomas Pommier, Mylene Hugoni, Yvan Moenne-Loccoz
Summary: Microorganisms extensively colonize caves and may cause damage to cave walls, particularly in caves displaying Paleolithic art. Research has shown that microbial phyla in pristine caves are similar to those in other ecosystems, but more specific studies are needed at lower taxonomic levels to clarify differences. Tourism can impact the microbiota of Paleolithic caves, with larger-scale investigations necessary to understand the extent of these shifts.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Lise Alonso, Thomas Pommier, Danis Abrouk, Mylene Hugoni, Van Tran Van, Guillaume Minard, Claire Valiente Moro, Yvan Moenne-Loccoz
Summary: The dark zones in Lascaux Cave have distinct microbial communities compared to the black stains, although they share some similarities. There is a possibility that insects play a role in promoting both types of microbial alterations on the cave walls.
Article
Genetics & Heredity
Zelia Bontemps, Claire Prigent-Combaret, Alice Guillmot, Mylene Hugoni, Yvan Moenne-Loccoz
Summary: This study examines the microbial community diversity in the Lascaux cave, focusing on the differences between unmarked surfaces and dark zones. The results show distinct microbial communities between the two types of surfaces, with dark zones harboring both cosmopolitan taxa and dark zone-specific taxa. Additionally, microbial proliferation in dark zones is observed.
ENVIRONMENTAL MICROBIOME
(2023)
Article
Biochemistry & Molecular Biology
Maxime Bruto, Philippe M. Oger, Patrice Got, Cecile Bernard, Delphine Melayah, Lilian A. Cloarec, Charlotte Duval, Arthur Escalas, Sebastien Duperron, Ludivine Guigard, Christophe Leboulanger, Magali Ader, Gerard Sarazin, Didier Jezequel, Helene Agogue, Marc Troussellier, Mylene Hugoni
Summary: Microbial diversity in the phycosphere, the region surrounding phytoplankton cells, was explored in a hypersaline, hyperalkaline ecosystem. Contrasting compositions of archaeal communities were found in the phycosphere of different phytoplanktonic species, indicating the influence of phytoplankton on surrounding archaea. Some archaeal groups were enriched in the phycosphere, possibly due to the physical impact of phytoplanktonic species.
